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Article ; Online: Evaluation of semitendinosus muscle transposition for treatment of perineal hernias in 33 small-breed dogs.

Hashimoto, Yuko / Nakagawa, Takayuki / Nishimura, Ryohei

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire

2023 Volume 87, Issue 4, Page(s) 282–289

Abstract: Little is known about the perioperative complications and treatment outcomes of repairing perineal hernias using semitendinosus muscle transposition (SMT) in small-breed dogs. The objective of this study was to retrospectively evaluate the complications ... ...

Abstract	Little is known about the perioperative complications and treatment outcomes of repairing perineal hernias using semitendinosus muscle transposition (SMT) in small-breed dogs. The objective of this study was to retrospectively evaluate the complications and prognosis of perineal hernia repair using SMT in 33 small, male dogs (weighing < 15 kg). Before the study began, the medical records of all small-breed dogs that underwent SMT from March 2013 to June 2022 at the Veterinary Medical Center, University of Tokyo were analyzed, and telephone interviews were conducted with their owners or referring physicians. Short-term complications were observed in 42.4% of the patients. Lameness was the most common complication, although all cases were resolved within a few days. The recurrence rate during the study period (median: 265 d) was 9.1%. The results of this study showed that transposition of the semitendinosus muscle is more invasive than other surgical procedures for perineal hernias. It is, however, an effective alternative when repair with internal obturator muscle transposition alone is expected to be difficult, such as severe atrophy of the internal obturator muscle or herniation in the ventral direction, and in cases with a history of surgery by transposition of the internal obturator muscle.
MeSH term(s)	Animals ; Dogs ; Male ; Dog Diseases/surgery ; Hamstring Muscles ; Hernia/veterinary ; Perineum/surgery ; Retrospective Studies
Language	English
Publishing date	2023-10-01
Publishing country	Canada
Document type	Journal Article
ZDB-ID	632814-3
ISSN	1928-9022 ; 0830-9000
ISSN (online)	1928-9022
ISSN	0830-9000
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Article ; Online: Effects of remifentanil on the noxiously stimulated somatosensory evoked potentials recorded at the spinal cord in dogs and cats

Nagakubo, Dai / Muta, Kanako / Kamata, Masatoshi / Iizuka, Tomoya / Fujita, Naoki / Nakagawa, Takayuki / Nishimura, Ryohei

Research in Veterinary Science. 2023 May, v. 158 p.13-16

2023

Abstract: This study assessed the somatosensory evoked potentials (SEPs) in dogs and cats to compare the effect of remifentanil on the action potentials evoked by peripheral noxious stimulation in the spinal cord. Five healthy dogs and five healthy cats underwent ... ...

Abstract	This study assessed the somatosensory evoked potentials (SEPs) in dogs and cats to compare the effect of remifentanil on the action potentials evoked by peripheral noxious stimulation in the spinal cord. Five healthy dogs and five healthy cats underwent general anaesthesia induced with propofol and maintained with isoflurane. Each animals received all dosage of a constant-rate infusion of remifentanil at 0 (control), 0.25, 0.5, 1.0 or 2.0 μg/kg/min. The hair of the dorsal foot of a hind limb was clipped and an intraepidermal stimulation electrode that could selectively stimulate the nociceptive Aδ and C fibres was attached. An electrical stimulus was generated by a portable peripheral nerve testing device. The evoked potentials were recorded by two needle electrodes inserted subcutaneously in the dorsal midline between the lumbar vertebra: L3-L4 and L4-L5. Bimodal waveforms were obtained by electrical stimulation in control dogs and cats. The inhibitory effect of remifentanil was evaluated by comparing the changes in the N1P2 and P2N2 amplitudes. The N1P2 amplitude was depressed by remifentanil in a dose-dependent manner in dogs, but it showed no remifentanil-induced changes in cats. While the P2N2 amplitude was also depressed in a dose-dependent manner in dogs, it showed milder remifentanil-induced effects in cats. The N1P2 and P2N2 amplitudes observed herein are assumed to represent the evoked potentials derived from the Aδ and C fibres, respectively. Thus, the inhibitory effect of remifentanil on nociceptive transmission at the spinal cord was much weaker in cats, especially for transmissions possibly derived from Aδ fibres.
Keywords	anesthesia ; dose response ; electrical treatment ; electrodes ; hindlimbs ; isoflurane ; nerve tissue ; research ; spinal cord ; vertebrae ; veterinary medicine ; Analgesia ; Canine ; Feline ; μ-Opioid ; Pain ; Somatosensory evoked potential
Language	English
Dates of publication	2023-05
Size	p. 13-16.
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	840961-4
ISSN	1532-2661 ; 0034-5288
ISSN (online)	1532-2661
ISSN	0034-5288
DOI	10.1016/j.rvsc.2023.03.001
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Article ; Online: Assessment of changes in blood pancreatic lipase activities using FDC-v-LIP in dogs that underwent various surgical procedures.

Saeki, Kanna / Saeki, Kohei / Yokoyama, Nozomu / Ohno, Koichi / Nishimura, Ryohei

Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)

2022 Volume 32, Issue 4, Page(s) 471–478

Abstract: Objective: To describe the perioperative changes in blood pancreatic lipase activity and explore the contributing clinical factors associated with these changes.: Design: Prospective observational study.: Setting: University teaching hospital.: ... ...

Abstract	Objective: To describe the perioperative changes in blood pancreatic lipase activity and explore the contributing clinical factors associated with these changes. Design: Prospective observational study. Setting: University teaching hospital. Animals: One hundred and four dogs underwent various surgical procedures under general anesthesia. Interventions: None. Measurements and main results: Blood pancreatic lipase activities, which were measured using FUJI DRI-CHEM v-Lip-P (FDC-v-Lip), significantly increased postoperatively compared to preoperative measurements (premedian 58.5 U/L [range, 23-157] vs. postmedian 80 U/L [range, 22-1000], P < 0.0001). The patient with a postoperative increase in FDC-v-Lip over the normal range (35 dogs [33.6%]) had significantly higher preoperative FDC-v-Lip values. Conclusions: In this study, dogs had significantly increased pancreas-specific lipase activities after surgical procedures under general anesthesia. Direct contributors to the increase and its relevance to clinical and histological pancreatitis should be determined in the future.
MeSH term(s)	Animals ; Dogs ; Dog Diseases/surgery ; Lipase ; Pancreas/surgery ; Pancreatitis/veterinary ; Reference Values
Chemical Substances	Lipase (EC 3.1.1.3)
Language	English
Publishing date	2022-04-20
Publishing country	United States
Document type	Journal Article ; Observational Study, Veterinary
ZDB-ID	2077212-9
ISSN	1476-4431 ; 1479-3261
ISSN (online)	1476-4431
ISSN	1479-3261
DOI	10.1111/vec.13195
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Article ; Online: Detection of domestic cat hepadnavirus by next-generation sequencing and epidemiological survey in Japan.

Sakamoto, Haruka / Ito, Genta / Goto-Koshino, Yuko / Sakamoto, Megumi / Nishimura, Ryohei / Momoi, Yasuyuki

The Journal of veterinary medical science

2023 Volume 85, Issue 6, Page(s) 642–646

Abstract: The novel domestic cat hepadnavirus (DCH), a member of the Hepadnaviridae, was first detected in Australia and has recently been identified in more countries. In this study, we explored the DCH genome using next-generation sequencing of a plasma sample ... ...

Abstract	The novel domestic cat hepadnavirus (DCH), a member of the Hepadnaviridae, was first detected in Australia and has recently been identified in more countries. In this study, we explored the DCH genome using next-generation sequencing of a plasma sample from a cat with a fever of unknown cause. Nucleotide sequence analysis showed the virus to be relatively genetically distant from the first reported DCH in Australia, showing 89% homology. Then we conducted an epidemiological survey by PCR of plasma samples collected from 203 cats that visited a veterinary hospital for diagnosis and treatment. Two of the 203 surveyed cats a were positive for DCH. One of the two positive cases had elevated liver enzymes of unknown etiology, and the other had hepatocellular adenoma. Our study indicated that DCH infection was observed in domestic cats in the Tokyo area of Japan as well as other reported areas in the world. Further investigations are needed to define the clinical importance of DCH.
MeSH term(s)	Animals ; Cats ; Japan/epidemiology ; Hepadnaviridae/genetics ; Tokyo ; High-Throughput Nucleotide Sequencing/veterinary ; Cat Diseases/diagnosis ; Cat Diseases/epidemiology
Language	English
Publishing date	2023-05-15
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.22-0439
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Article ; Online: Effects of remifentanil on the noxiously stimulated somatosensory evoked potentials recorded at the spinal cord in dogs and cats.

Nagakubo, Dai / Muta, Kanako / Kamata, Masatoshi / Iizuka, Tomoya / Fujita, Naoki / Nakagawa, Takayuki / Nishimura, Ryohei

Research in veterinary science

2023 Volume 158, Page(s) 13–16

Abstract	This study assessed the somatosensory evoked potentials (SEPs) in dogs and cats to compare the effect of remifentanil on the action potentials evoked by peripheral noxious stimulation in the spinal cord. Five healthy dogs and five healthy cats underwent general anaesthesia induced with propofol and maintained with isoflurane. Each animals received all dosage of a constant-rate infusion of remifentanil at 0 (control), 0.25, 0.5, 1.0 or 2.0 μg/kg/min. The hair of the dorsal foot of a hind limb was clipped and an intraepidermal stimulation electrode that could selectively stimulate the nociceptive Aδ and C fibres was attached. An electrical stimulus was generated by a portable peripheral nerve testing device. The evoked potentials were recorded by two needle electrodes inserted subcutaneously in the dorsal midline between the lumbar vertebra: L3-L4 and L4-L5. Bimodal waveforms were obtained by electrical stimulation in control dogs and cats. The inhibitory effect of remifentanil was evaluated by comparing the changes in the N1P2 and P2N2 amplitudes. The N1P2 amplitude was depressed by remifentanil in a dose-dependent manner in dogs, but it showed no remifentanil-induced changes in cats. While the P2N2 amplitude was also depressed in a dose-dependent manner in dogs, it showed milder remifentanil-induced effects in cats. The N1P2 and P2N2 amplitudes observed herein are assumed to represent the evoked potentials derived from the Aδ and C fibres, respectively. Thus, the inhibitory effect of remifentanil on nociceptive transmission at the spinal cord was much weaker in cats, especially for transmissions possibly derived from Aδ fibres.
MeSH term(s)	Cats ; Dogs ; Animals ; Remifentanil/pharmacology ; Cat Diseases ; Dog Diseases ; Evoked Potentials, Somatosensory/physiology ; Spinal Cord ; Evoked Potentials
Chemical Substances	Remifentanil (P10582JYYK)
Language	English
Publishing date	2023-03-05
Publishing country	England
Document type	Journal Article
ZDB-ID	840961-4
ISSN	1532-2661 ; 0034-5288
ISSN (online)	1532-2661
ISSN	0034-5288
DOI	10.1016/j.rvsc.2023.03.001
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Article: Assessment of changes in blood pancreatic lipase activities using FDC‐v‐LIP in dogs that underwent various surgical procedures

Saeki, Kanna / Saeki, Kohei / Yokoyama, Nozomu / Ohno, Koichi / Nishimura, Ryohei

Journal of veterinary emergency and critical care. 2022 July, v. 32, no. 4

2022

Abstract: OBJECTIVE: To describe the perioperative changes in blood pancreatic lipase activity and explore the contributing clinical factors associated with these changes. DESIGN: Prospective observational study. SETTING: University teaching hospital. ANIMALS: One ...

Abstract	OBJECTIVE: To describe the perioperative changes in blood pancreatic lipase activity and explore the contributing clinical factors associated with these changes. DESIGN: Prospective observational study. SETTING: University teaching hospital. ANIMALS: One hundred and four dogs underwent various surgical procedures under general anesthesia. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Blood pancreatic lipase activities, which were measured using FUJI DRI‐CHEM v‐Lip‐P (FDC‐v‐Lip), significantly increased postoperatively compared to preoperative measurements (premedian 58.5 U/L [range, 23–157] vs. postmedian 80 U/L [range, 22–1000], P < 0.0001). The patient with a postoperative increase in FDC‐v‐Lip over the normal range (35 dogs [33.6%]) had significantly higher preoperative FDC‐v‐Lip values. CONCLUSIONS: In this study, dogs had significantly increased pancreas‐specific lipase activities after surgical procedures under general anesthesia. Direct contributors to the increase and its relevance to clinical and histological pancreatitis should be determined in the future.
Keywords	anesthesia ; blood ; histology ; hospitals ; observational studies ; pancreatitis ; patients ; triacylglycerol lipase
Language	English
Dates of publication	2022-07
Size	p. 471-478.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2077212-9
ISSN	1476-4431 ; 1479-3261
ISSN (online)	1476-4431
ISSN	1479-3261
DOI	10.1111/vec.13195
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The profile of urinary lipid metabolites in healthy dogs.

Kida, Taiki / Yamazaki, Arisa / Kobayashi, Koji / Nakamura, Tatsuro / Nakagawa, Takayuki / Nishimura, Ryohei / Murata, Takahisa

The Journal of veterinary medical science

2022 Volume 84, Issue 5, Page(s) 644–647

Abstract: Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through ... ...

Abstract	Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases.
MeSH term(s)	Animals ; Arachidonic Acid ; Chromatography, Liquid/veterinary ; Cytochrome P-450 Enzyme System ; Docosahexaenoic Acids/metabolism ; Dogs ; Eicosanoids ; Eicosapentaenoic Acid/metabolism ; Prostaglandin-Endoperoxide Synthases ; Tandem Mass Spectrometry/veterinary
Chemical Substances	Eicosanoids ; Docosahexaenoic Acids (25167-62-8) ; Arachidonic Acid (27YG812J1I) ; Cytochrome P-450 Enzyme System (9035-51-2) ; Eicosapentaenoic Acid (AAN7QOV9EA) ; Prostaglandin-Endoperoxide Synthases (EC 1.14.99.1)
Language	English
Publishing date	2022-03-23
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.22-0020
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Article ; Online: Canine bone marrow peri-adipocyte cells could therapeutically benefit acute spinal cord injury through migration and secretion of hepatocyte growth factor to inflammatory milieu.

Chen, Junyan / Fujita, Naoki / Takeda, Tae / Hanyu, Wataru / Takatani, Hirohide / Nakagawa, Takayuki / Nishimura, Ryohei

Experimental animals

2022 Volume 72, Issue 1, Page(s) 19–29

Abstract: Spinal cord injury (SCI) is a common neurological disorder in dogs. A secondary injury that occurs in the acute phase causes expansion of inflammation, resulting in lesion extension and further loss of function. Mesenchymal stem cells (MSCs) have trophic ...

Abstract	Spinal cord injury (SCI) is a common neurological disorder in dogs. A secondary injury that occurs in the acute phase causes expansion of inflammation, resulting in lesion extension and further loss of function. Mesenchymal stem cells (MSCs) have trophic effects and the ability to migrate toward injured tissues; therefore, MSC-based therapy is considered promising for the treatment of canine SCI. We recently reported that bone marrow peri-adipocyte cells (BM-PACs) can be obtained from canine bone marrow and have stem cell potential superior to that of conventional bone marrow MSCs (BMMSCs). However, their therapeutic potential for SCI have been still unknow. Here, we first evaluated the ability of BM-PACs to secrete hepatocyte growth factor (HGF) and their migration ability toward inflammatory milieu in vitro. BM-PACs can secrete HGF in response to pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and IL-1β, and exhibit migration ability toward these cytokines. Next, BM-PACs were intravenously administered into nude mice with acute SCI to analyze the homing ability and therapeutic effects of HGF secreted by BM-PACs. BM-PACs homed to the injured spinal cord, where the HGF expression level increased 7 days after administration. Intravenous administration of BM-PACs induced functional recovery and pathological improvement, indicated by less demyelinating area, more preserved axons, and less glial scar formation compared with the mice only received vehicle. These findings suggest that the intravenous administration of BM-PACs can be a novel therapeutic intervention for acute canine SCI.
MeSH term(s)	Animals ; Dogs ; Mice ; Hepatocyte Growth Factor ; Bone Marrow ; Mice, Nude ; Spinal Cord Injuries ; Spinal Cord/pathology ; Adipocytes ; Bone Marrow Cells/metabolism
Chemical Substances	Hepatocyte Growth Factor (67256-21-7)
Language	English
Publishing date	2022-08-15
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2088228-2
ISSN	1881-7122 ; 1341-1357 ; 0007-5124
ISSN (online)	1881-7122
ISSN	1341-1357 ; 0007-5124
DOI	10.1538/expanim.22-0026
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Article ; Online: Effect of Fibroblast Growth Factor-2 and Serum on Canine Mesenchymal Stem Cell Chondrogenesis.

Endo, Kentaro / Fujita, Naoki / Nakagawa, Takayuki / Nishimura, Ryohei

Tissue engineering. Part A

2019 Volume 25, Issue 11-12, Page(s) 901–910

Abstract: Impact statement: Tissue engineering using the chondrogenic potential of mesenchymal stem cells (MSCs) is a promising approach for cartilage regenerative therapy. Although dogs are widely used as an animal model for cartilage regeneration, chondrogenic ... ...

Abstract	Impact statement: Tissue engineering using the chondrogenic potential of mesenchymal stem cells (MSCs) is a promising approach for cartilage regenerative therapy. Although dogs are widely used as an animal model for cartilage regeneration, chondrogenic differentiation of canine MSCs is still challenging. In this study, we aimed at establishing the optimal conditions for canine MSC chondrogenesis. Our results demonstrated that preconditioning with fibroblast growth factor-2 and serum-free induction medium enabled robust chondrogenesis of canine MSCs. These findings will allow effective generation of cartilage tissue from canine MSCs and advance research of cartilage regeneration in both dogs and humans.
MeSH term(s)	Animals ; Cattle ; Cell Differentiation/drug effects ; Chondrogenesis/drug effects ; Dogs ; Female ; Fibroblast Growth Factor 2/pharmacology ; Male ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Serum ; Spheroids, Cellular/cytology ; Spheroids, Cellular/metabolism
Chemical Substances	Fibroblast Growth Factor 2 (103107-01-3)
Language	English
Publishing date	2019-05-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2420582-5
ISSN	1937-335X ; 1937-3341
ISSN (online)	1937-335X
ISSN	1937-3341
DOI	10.1089/ten.TEA.2018.0177
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Article ; Online: Comparison of the effect of growth factors on chondrogenesis of canine mesenchymal stem cells.

Endo, Kentaro / Fujita, Naoki / Nakagawa, Takayuki / Nishimura, Ryohei

The Journal of veterinary medical science

2019 Volume 81, Issue 8, Page(s) 1211–1218

Abstract: Mesenchymal stem cells (MSCs) are proposed to be useful in cartilage regenerative medicine, however, canine MSCs have been reported to show poor chondrogenic capacity. Therefore, optimal conditions for chondrogenic differentiation should be determined by ...

Abstract	Mesenchymal stem cells (MSCs) are proposed to be useful in cartilage regenerative medicine, however, canine MSCs have been reported to show poor chondrogenic capacity. Therefore, optimal conditions for chondrogenic differentiation should be determined by mimicking the developmental process. We have previously established novel and superior canine MSCs named bone marrow peri-adipocyte cells (BM-PACs) and the objective of this study was to evaluate the effects of growth factors required for in vivo chondrogenesis using canine BM-PACs. Spheroids of BM-PACs were cultured in chondrogenic medium containing 10 ng/ml transforming growth factor-β1 (TGF-β1) with or without 100 ng/ml bone morphogenetic protein-2 (BMP-2), 100 ng/ml growth differentiation factor-5 (GDF-5) or 100 ng/ml insulin-like growth factor-1 (IGF-1). Chondrogenic differentiation was evaluated by the quantification of glycosaminoglycan and Safranin O staining for proteoglycan production. The expression of cartilage matrix or hypertrophic gene/protein was also evaluated by qPCR and immunohistochemistry. Spheroids in all groups were strongly stained with Safranin O. Although BMP-2 significantly increased glycosaminoglycan production, Safranin O-negative outer layer was formed and the mRNA expression of COL10 relating to cartilage hypertrophy was also significantly upregulated (P<0.05). GDF-5 promoted the production of glycosaminoglycan and type II collagen without increasing COL10 mRNA expression. The supplementation of IGF-1 did not significantly affect cartilaginous and hypertrophic differentiation. Our results indicate that GDF-5 is a useful growth factor for the generation of articular cartilage from canine MSCs.
MeSH term(s)	Animals ; Bone Morphogenetic Proteins/metabolism ; Bone Morphogenetic Proteins/pharmacology ; Cartilage, Articular/drug effects ; Cartilage, Articular/metabolism ; Cells, Cultured/metabolism ; Chondrogenesis/drug effects ; Collagen Type II/drug effects ; Collagen Type II/metabolism ; Dogs ; Glycosaminoglycans/metabolism ; Growth Differentiation Factors/metabolism ; Growth Differentiation Factors/pharmacology ; Insulin-Like Growth Factor I/analogs & derivatives ; Insulin-Like Growth Factor I/metabolism ; Insulin-Like Growth Factor I/pharmacology ; Intercellular Signaling Peptides and Proteins/metabolism ; Intercellular Signaling Peptides and Proteins/pharmacology ; Mesenchymal Stem Cells/drug effects ; Mesenchymal Stem Cells/metabolism ; Tissue Engineering ; Transforming Growth Factor beta/metabolism ; Transforming Growth Factor beta/pharmacology
Chemical Substances	Bone Morphogenetic Proteins ; Collagen Type II ; Glycosaminoglycans ; Growth Differentiation Factors ; Intercellular Signaling Peptides and Proteins ; Transforming Growth Factor beta ; insulin-like growth factor-1 D peptide ; Insulin-Like Growth Factor I (67763-96-6)
Language	English
Publishing date	2019-06-04
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.18-0551
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