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  1. Article ; Online: Role of c‐Jun NH2‐terminal kinase‐mediated mitogen‐activated protein kinase pathway in response to pesticides in Apis cerana cerana

    Niu, Xiao‐Jing / Wang, Li‐Jun / Meng, Hui / Wang, Hong‐Fang / Xu, Bao‐Hua / Wang, Chen

    Insect Science. 2023 Feb., v. 30, no. 1 p.47-64

    2023  

    Abstract: The mitogen‐activated protein kinase (MAPK) cascade pathway plays an important role in regulating stress responses. The function of the c‐Jun NH₂‐terminal kinase (JNK), a component of the MAPK cascade pathway, in Apis cerana cerana (Acc) remains unclear. ...

    Abstract The mitogen‐activated protein kinase (MAPK) cascade pathway plays an important role in regulating stress responses. The function of the c‐Jun NH₂‐terminal kinase (JNK), a component of the MAPK cascade pathway, in Apis cerana cerana (Acc) remains unclear. Here, JNK was isolated and identified from Acc. Bioinformatics analyses revealed there is a typical serine/threonine protein kinase catalytic domain in the AccJNK protein. An expression profile analysis showed that AccJNK was significantly induced by pesticide treatments. To further explore the functional mechanisms of AccJNK, a yeast 2‐hybrid screen was performed, activator protein‐1 (AP‐1) was screened as the interaction partner of AccJNK, and the interaction relationship was further verified by pull‐down assay. Quantitative real‐time polymerase chain reaction showed the expression pattern of AccAP‐1 was similar to that of AccJNK. After a knockdown of AccJNK or AccAP‐1 by RNA interference, the survival rate of Acc after pesticide treatments increased. Additionally, the expression levels of antioxidant‐related genes and the activities of antioxidant enzymes increased, suggesting that the knockdown of AccJNK or AccAP‐1 increased the antioxidant capacity of bees. Our study revealed that the JNK‐mediated MAPK pathway responds to pesticide stress by altering the antioxidant capacity of Acc.
    Keywords Apis cerana ; RNA interference ; active sites ; antioxidant activity ; bioinformatics ; insects ; mitogen-activated protein kinase ; pesticides ; quantitative polymerase chain reaction ; serine ; survival rate ; threonine ; yeasts
    Language English
    Dates of publication 2023-02
    Size p. 47-64.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 2179775-4
    ISSN 1744-7917 ; 1672-9609
    ISSN (online) 1744-7917
    ISSN 1672-9609
    DOI 10.1111/1744-7917.13053
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  2. Article ; Online: Role of c-Jun NH

    Niu, Xiao-Jing / Wang, Li-Jun / Meng, Hui / Wang, Hong-Fang / Xu, Bao-Hua / Wang, Chen

    Insect science

    2022  Volume 30, Issue 1, Page(s) 47–64

    Abstract: The mitogen-activated protein kinase (MAPK) cascade pathway plays an important role in regulating stress responses. The function of the c-Jun ... ...

    Abstract The mitogen-activated protein kinase (MAPK) cascade pathway plays an important role in regulating stress responses. The function of the c-Jun NH
    MeSH term(s) Bees ; Animals ; Antioxidants/metabolism ; Transcription Factor AP-1 ; Mitogen-Activated Protein Kinases ; Pesticides ; Insect Proteins/genetics
    Chemical Substances Antioxidants ; Transcription Factor AP-1 ; Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; Pesticides ; Insect Proteins
    Language English
    Publishing date 2022-06-01
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2179775-4
    ISSN 1744-7917 ; 1672-9609
    ISSN (online) 1744-7917
    ISSN 1672-9609
    DOI 10.1111/1744-7917.13053
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Fox transcription factor AccGRF1 in response to glyphosate stress in Apis cerana cerana.

    Niu, Xiao-Jing / Sun, Yun-Hao / Wang, Li-Jun / Huang, Yuan-Yuan / Wang, Ying / Guo, Xing-Qi / Xu, Bao-Hua / Wang, Chen

    Pesticide biochemistry and physiology

    2023  Volume 192, Page(s) 105419

    Abstract: Glyphosate is an herbicide commonly used in agriculture, and its widespread use has adversely affected the survival of nontarget organisms. Among these organisms, bees in particular are important pollinators, and declining bee populations have severely ... ...

    Abstract Glyphosate is an herbicide commonly used in agriculture, and its widespread use has adversely affected the survival of nontarget organisms. Among these organisms, bees in particular are important pollinators, and declining bee populations have severely affected crop yields around the world. However, the molecular mechanism by which glyphosate harms bees remains unclear. In our experiment, we screened and cloned a glyphosate-induced gene in Apis cerana cerana (A. c. cerana) and named glyphosate response factor 1 (AccGRF1). Sequence analysis showed that AccGRF1 contains a winged-helix DNA binding domain, which suggests that it belongs to the Forkhead box (Fox) protein family. qRT-PCR and heterologous expression in Escherichia coli and yeast showed that AccGRF1 can respond to glyphosate and oxidative stress. After AccGRF1 knockdown by means of RNA interference (RNAi), the resistance of A. c. cerana to glyphosate stress improved. The results suggested that AccGRF1 is involved in A. c. cerana glyphosate stress tolerance. This study reveals the functions of Fox transcription factors in response to glyphosate stress and provides molecular insights into the regulation of glyphosate responses in honeybees.
    MeSH term(s) Bees/genetics ; Animals ; Oxidative Stress/genetics ; RNA Interference ; Glycine/toxicity ; Insect Proteins/metabolism ; Glyphosate
    Chemical Substances Glycine (TE7660XO1C) ; Insect Proteins
    Language English
    Publishing date 2023-04-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 184819-7
    ISSN 1095-9939 ; 0048-3583 ; 0048-3575
    ISSN (online) 1095-9939
    ISSN 0048-3583 ; 0048-3575
    DOI 10.1016/j.pestbp.2023.105419
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Fox transcription factor AccGRF1 in response to glyphosate stress in Apis cerana cerana

    Niu, Xiao-Jing / Sun, Yun-Hao / Wang, Li-Jun / Huang, Yuan-Yuan / Wang, Ying / Guo, Xing-Qi / Xu, Bao-Hua / Wang, Chen

    Pesticide Biochemistry and Physiology. 2023 May, v. 192 p.105419-

    2023  

    Abstract: Glyphosate is an herbicide commonly used in agriculture, and its widespread use has adversely affected the survival of nontarget organisms. Among these organisms, bees in particular are important pollinators, and declining bee populations have severely ... ...

    Abstract Glyphosate is an herbicide commonly used in agriculture, and its widespread use has adversely affected the survival of nontarget organisms. Among these organisms, bees in particular are important pollinators, and declining bee populations have severely affected crop yields around the world. However, the molecular mechanism by which glyphosate harms bees remains unclear. In our experiment, we screened and cloned a glyphosate-induced gene in Apis cerana cerana (A. c. cerana) and named glyphosate response factor 1 (AccGRF1). Sequence analysis showed that AccGRF1 contains a winged-helix DNA binding domain, which suggests that it belongs to the Forkhead box (Fox) protein family. qRT–PCR and heterologous expression in Escherichia coli and yeast showed that AccGRF1 can respond to glyphosate and oxidative stress. After AccGRF1 knockdown by means of RNA interference (RNAi), the resistance of A. c. cerana to glyphosate stress improved. The results suggested that AccGRF1 is involved in A. c. cerana glyphosate stress tolerance. This study reveals the functions of Fox transcription factors in response to glyphosate stress and provides molecular insights into the regulation of glyphosate responses in honeybees.
    Keywords Apis cerana ; DNA ; Escherichia coli ; RNA interference ; bees ; genes ; glyphosate ; heterologous gene expression ; oxidative stress ; physiology ; sequence analysis ; stress tolerance ; transcription factors ; yeasts ; Apis cerana cerana ; Fox transcription factor
    Language English
    Dates of publication 2023-05
    Publishing place Elsevier Inc.
    Document type Article ; Online
    ZDB-ID 184819-7
    ISSN 1095-9939 ; 0048-3583 ; 0048-3575
    ISSN (online) 1095-9939
    ISSN 0048-3583 ; 0048-3575
    DOI 10.1016/j.pestbp.2023.105419
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3585: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article: Large eddy simliation wave breaking over muddy seabed

    HU, Yi / NIU, Xiao-jing / YU, Xi-ping

    Journal of Hydrodynamics, Ser.B. 2012 Apr., v. 24, no. 2

    2012  

    Abstract: The Large Eddy Simulation (LES) of the wave breaking over a muddy seabed is carried out with a Coupled Level Set and Volume Of Fluid (CLSVOF) method to capture the interfaces. The effects of the mud on the wave breaking are studied. The existence of a ... ...

    Abstract The Large Eddy Simulation (LES) of the wave breaking over a muddy seabed is carried out with a Coupled Level Set and Volume Of Fluid (CLSVOF) method to capture the interfaces. The effects of the mud on the wave breaking are studied. The existence of a mud layer beneath an otherwise rigid bottom is found to have a similar effect as an increase of the water depth. As compared with the case of a simple rigid bottom, the inception of the wave breaking is evidently delayed and the breaking intensity is much reduced. The dissipation of the wave energy is shown to have very different rates before, during and after the breaking. Before and after the breaking, the mud plays an important role. During the breaking, however, the turbulence as well as the entrainment of the air also dissipate a large amount of energy.
    Keywords air ; energy ; turbulent flow ; water power
    Language English
    Dates of publication 2012-04
    Size p. 298-304.
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 1001-6058
    DOI 10.1016/S1001-6058(11)60248-5
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