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  1. Article ; Online: Ultrasensitive qPCR-Based Detection of Plasmodium falciparum in Pregnant Women Using Dried Blood or Whole Blood Pellet Samples Processed through Different DNA Extraction Methods.

    Saidi, Queen / Minja, Daniel / Njau, Judith / Hansson, Helle / Kavishe, Reginald / Alifrangis, Michael

    The American journal of tropical medicine and hygiene

    2021  Volume 106, Issue 3, Page(s) 846–849

    Abstract: Highly sensitive molecular techniques for the detection of low-level Plasmodium falciparum parasitemia are highly useful for various clinical and epidemiological studies. However, differences in how blood samples are preserved, the quantity of blood ... ...

    Abstract Highly sensitive molecular techniques for the detection of low-level Plasmodium falciparum parasitemia are highly useful for various clinical and epidemiological studies. However, differences in how blood samples are preserved, the quantity of blood stored, as well as genomic DNA extraction methods used may compromise the potential usefulness of these methodologies. This study compared diagnostic sensitivity based on microscopy and malaria rapid diagnostic tests (mRDTs), with quantitative polymerase chain reaction (qPCR) P. falciparum positivity of dried blood spots (DBS) or whole blood pellets (WBP) from pregnant women using different DNA extraction protocols (Chelex-saponin or a commercial kit). Samples from 129 pregnant women were analyzed, of which 13 were P. falciparum positive by mRDT and 5 by microscopy. By using extraction kit on WBP and on DBS, qPCR positivity was 27 (20.9%) and 16 (12.4%), respectively, whereas Chelex extraction on DBS only resulted in 4 (3.1%) P. falciparum positive samples. Thus, extraction using commercial kits greatly improve the likelihood of detecting P. falciparum infections.
    MeSH term(s) DNA ; Female ; Humans ; Malaria/diagnosis ; Malaria, Falciparum/diagnosis ; Plasmodium falciparum/genetics ; Pregnancy ; Pregnant Women ; Sensitivity and Specificity
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2021-12-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2942-7
    ISSN 1476-1645 ; 0002-9637
    ISSN (online) 1476-1645
    ISSN 0002-9637
    DOI 10.4269/ajtmh.21-0496
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: Complete genome sequence of

    Mbwambo, Gershom A / van Zwetselaar, Marco / Sonda, Tolbert / Lukambagire, AbdulHamid S / Njau, Judith S / Wadugu, Boaz / Ignass, Ignass P / Amani, Nelson B / Hugho, Ephrasia A / Rubach, Matthew P / Sakasaka, Philoteus / Oisso, Rose S / Mkenda, Nestory / Shirima, Gabriel / Ashford, Roland T / Haydon, Daniel T / Maro, Venance P / Kazwala, Rudovick R / Kumburu, Happiness H /
    Mmbaga, Blandina T / Halliday, Jo E B

    Microbiology resource announcements

    2024  Volume 13, Issue 2, Page(s) e0093023

    Abstract: ... Brucella ... ...

    Abstract Brucella abortus
    Language English
    Publishing date 2024-01-30
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.00930-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Nanopore sequencing technology for clinical diagnosis of infectious diseases where laboratory capacity is meager: A case report.

    Kumburu, Happiness H / Shayo, Mariana / van Zwetslaar, Marco / Njau, Judith / Kuchaka, Davis J / Ignas, Ignas P / Wadugu, Boaz / Kasworm, Robert / Masaki, Lazaro J / Hallgren, Malte B / Clausen, Philip T L C / Mmbaga, Blandina Theophil / Aarestrup, Frank M / Sonda, Tolbert B

    Heliyon

    2023  Volume 9, Issue 7, Page(s) e17439

    Abstract: In resource-limited settings, patients are often first presented to clinical settings when seriously ill and access to proper clinical microbial diagnostics is often very limited or non-existing. On February ... ...

    Abstract In resource-limited settings, patients are often first presented to clinical settings when seriously ill and access to proper clinical microbial diagnostics is often very limited or non-existing. On February 16th
    Language English
    Publishing date 2023-06-28
    Publishing country England
    Document type Case Reports
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2023.e17439
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Genetic and nongenetic drivers of platelet reactivity in healthy Tanzanian individuals.

    Kullaya, Vesla I / Temba, Godfrey S / Vadaq, Nadira / Njau, Judith / Boahen, Collins K / Nkambule, Bongani B / Thibord, Florian / Chen, Ming-Huei / Pecht, Tal / Lyamuya, Furaha / Kumar, Vinod / Netea, Mihai G / Mmbaga, Blandina T / van der Ven, Andre / Johnson, Andrew D / de Mast, Quirijn

    Journal of thrombosis and haemostasis : JTH

    2023  Volume 22, Issue 3, Page(s) 805–817

    Abstract: Background: Platelets play a key role in hemostasis, inflammation, and cardiovascular diseases. Platelet reactivity is highly variable between individuals. The drivers of this variability in populations from Sub-Saharan Africa remain largely unknown.: ...

    Abstract Background: Platelets play a key role in hemostasis, inflammation, and cardiovascular diseases. Platelet reactivity is highly variable between individuals. The drivers of this variability in populations from Sub-Saharan Africa remain largely unknown.
    Objectives: We aimed to investigate the nongenetic and genetic determinants of platelet reactivity in healthy adults living in a rapidly urbanizing area in Northern Tanzania.
    Methods: Platelet activation and reactivity were measured by platelet P-selectin expression and the binding of fibrinogen in unstimulated blood and after ex vivo stimulation with adenosine diphosphate and PAR-1 and PAR-4 ligands. We then analyzed the associations of platelet parameters with host genetic and nongenetic factors, environmental factors, plasma inflammatory markers, and plasma metabolites.
    Results: Only a few associations were found between platelet reactivity parameters and plasma inflammatory markers and nongenetic host and environmental factors. In contrast, untargeted plasma metabolomics revealed a large number of associations with food-derived metabolites, including phytochemicals that were previously reported to inhibit platelet reactivity. Genome-wide single-nucleotide polymorphism genotyping identified 2 novel single-nucleotide polymorphisms (rs903650 and rs4789332) that were associated with platelet reactivity at the genome-wide level (P < 5 × 10
    Conclusion: Our study uncovered factors that determine variation in platelet reactivity in a population in East Africa that is rapidly transitioning to an urban lifestyle, including the importance of genetic ancestry and the gradual abandoning of the traditional East African diet.
    MeSH term(s) Adult ; Humans ; Platelet Aggregation/physiology ; Tanzania ; Blood Platelets/metabolism ; Platelet Activation ; Receptor, PAR-1/metabolism
    Chemical Substances Receptor, PAR-1
    Language English
    Publishing date 2023-11-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2112661-6
    ISSN 1538-7836 ; 1538-7933
    ISSN (online) 1538-7836
    ISSN 1538-7933
    DOI 10.1016/j.jtha.2023.11.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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