Article ; Online: Alterations of DNA methylation and expression of genes related to thyroid hormone metabolism in colon epithelium of obese patients.
2022 Volume 15, Issue 1, Page(s) 229
Abstract: Background: Colorectal cancer is common among obese individuals. The purpose of the current study was to determine changes in DNA methylation status and mRNA expression of thyroid hormone receptor beta (THRB), as a tumor suppressor, and thyroid hormone ... ...
| Abstract | Background: Colorectal cancer is common among obese individuals. The purpose of the current study was to determine changes in DNA methylation status and mRNA expression of thyroid hormone receptor beta (THRB), as a tumor suppressor, and thyroid hormone inactivating enzyme, type 3 deiodinase (DIO3) genes, in human epithelial colon tissues of healthy obese individuals. Methods: Colon biopsies were analyzed by methylation sensitive-high resolution melting (MS-HRM) to investigate promoter methylation of DIO3 and THRB, and by quantitative real-time polymerase chain reaction to assay expression of DIO3 and THRB mRNA on eighteen obese and twenty-one normal-weight healthy men. Results: There was no significant difference in mean methylation levels at the THRB promoter region between the two groups. Nevertheless, obesity decreased THRB expression levels, significantly (P < 0.05; fold change: 0.19). Furthermore, obesity attenuated DNA methylation (P < 0.001) and enhanced mRNA expression of DIO3 (P < 0.05; fold change: 3). Conclusions: Our findings suggest that obesity may alter expression of THRB and DIO3 genes through epigenetic mechanism. Alterations of THRB and DIO3 expressions may predispose colon epithelium of obese patients to neoplastic transformation. |
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| MeSH term(s) | Male ; Humans ; DNA Methylation ; Thyroid Hormone Receptors beta/genetics ; Thyroid Hormone Receptors beta/metabolism ; RNA, Messenger/genetics ; Thyroid Hormones/metabolism ; Obesity/genetics ; Colon/metabolism ; Epithelium/metabolism |
| Chemical Substances | Thyroid Hormone Receptors beta ; RNA, Messenger ; Thyroid Hormones |
| Language | English |
| Publishing date | 2022-11-01 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2411865-5 |
| ISSN | 1755-8794 ; 1755-8794 |
| ISSN (online) | 1755-8794 |
| ISSN | 1755-8794 |
| DOI | 10.1186/s12920-022-01387-6 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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