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Article: Increasing prevalence of celiac disease - where to look for answers?

Šťastná, M / Norek, A / Řádková, J / Sluková, M / Hrunka, M / Jabandžiev, P / Janda, L

Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne

2023 Volume 72, Issue 3, Page(s) 172–183

Abstract: Celiac disease or gluten-sensitive enteropathy is a relevant health concern in today's world. Three prerequisites need to be met to trigger the disease, namely a genetic predisposition, gluten consumption, and environmental factors. Retrospective studies ...

Title translation	Nárůst prevalence celiakie – kde hledat odpovědi?
Abstract	Celiac disease or gluten-sensitive enteropathy is a relevant health concern in today's world. Three prerequisites need to be met to trigger the disease, namely a genetic predisposition, gluten consumption, and environmental factors. Retrospective studies conducted across all age groups have ruled out the possibility that improved diagnostic methods were behind the increased prevalence. Since the genetic predisposition is more or less constant in the population, it is assumed that external factors may play a major role in this increase. Although it is generally believed that modern wheat varieties are to be blamed for the increase in gluten intolerance-related diseases, this assumption is refuted based on the analysis of the current and 100-year-old varieties. However, the increased prevalence could be related to modern lifestyles, changes in food preparation technology or composition, disruption of the intestinal barrier in viral disease, and other factors leading to intestinal dysbiosis. A possible preventive strategy in predisposed individuals could be the avoidance of gluten from the diet when ill, especially with a viral infection. This article openup a new perspective on the currently common autoimmune disease.
MeSH term(s)	Humans ; Celiac Disease/epidemiology ; Celiac Disease/diagnosis ; Genetic Predisposition to Disease ; Prevalence ; Retrospective Studies ; Glutens/genetics
Chemical Substances	Glutens (8002-80-0)
Language	English
Publishing date	2023-10-23
Publishing country	Czech Republic
Document type	Journal Article
ZDB-ID	1188216-5
ISSN	1805-451X ; 1210-7913 ; 0009-0522
ISSN (online)	1805-451X
ISSN	1210-7913 ; 0009-0522
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Article ; Online: Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold.

Norek, Adam / Janda, Lubomír

Protein science : a publication of the Protein Society

2017 Volume 26, Issue 4, Page(s) 796–806

Abstract: In current work, we used recombinant OspC protein derived from B. afzelii strain BRZ31 in the native homodimeric fold for mice immunization and following selection process to produce three mouse monoclonal antibodies able to bind to variable parts of up ... ...

Abstract	In current work, we used recombinant OspC protein derived from B. afzelii strain BRZ31 in the native homodimeric fold for mice immunization and following selection process to produce three mouse monoclonal antibodies able to bind to variable parts of up to five different OspC proteins. Applying the combination of mass spectrometry assisted epitope mapping and affinity based theoretical prediction we have localized regions responsible for antigen-antibody interactions and approximate epitopes' amino acid composition. Two mAbs (3F4 and 2A9) binds to linear epitopes located in previously described immunogenic regions in the exposed part of OspC protein. The third mAb (2D1) recognises highly conserved discontinuous epitope close to the ligand binding domain 1.
MeSH term(s)	Animals ; Antibodies, Monoclonal, Murine-Derived/chemistry ; Antibodies, Monoclonal, Murine-Derived/immunology ; Antigens, Bacterial/chemistry ; Antigens, Bacterial/genetics ; Antigens, Bacterial/immunology ; Bacterial Outer Membrane Proteins/chemistry ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/immunology ; Borrelia burgdorferi/chemistry ; Borrelia burgdorferi/genetics ; Borrelia burgdorferi/immunology ; Epitope Mapping ; Female ; Mice ; Mice, Inbred BALB C ; Protein Folding ; Protein Multimerization
Chemical Substances	Antibodies, Monoclonal, Murine-Derived ; Antigens, Bacterial ; Bacterial Outer Membrane Proteins ; OspC protein
Language	English
Publishing date	2017-02-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1106283-6
ISSN	1469-896X ; 0961-8368
ISSN (online)	1469-896X
ISSN	0961-8368
DOI	10.1002/pro.3125
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Article ; Online: Corrigendum to: DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic).

Norek, Adam / Janda, Lubomír / Žákovská, Alena

Journal of vector ecology : journal of the Society for Vector Ecology

2017 Volume 42, Issue 1, Page(s) 207

Language	English
Publishing date	2017-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	2212806-2
ISSN	1948-7134 ; 1081-1710
ISSN (online)	1948-7134
ISSN	1081-1710
DOI	10.1111/jvec.12260
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Article ; Online: DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic).

Norek, Adam / Janda, Lubomír / Žákovská, Alena

Journal of vector ecology : journal of the Society for Vector Ecology

2016 Volume 41, Issue 1, Page(s) 172–178

Abstract: Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in Pisárky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three ... ...

Abstract	Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in Pisárky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii, and B. valaisiana). Three samples from the collection contained more than one genospecies. In the other 15 strains, nucleotide sequences of flaB and ospC were determined. The following phylogenetic analysis assigned 12 isolates to genospecies B. garinii and three to B. afzelii. These isolates were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, and G5 (B. garinii) and A3 and A8 (B. afzelii).
MeSH term(s)	Animals ; Antigens, Bacterial/genetics ; Bacterial Outer Membrane Proteins/genetics ; Borrelia burgdorferi Group/classification ; Czech Republic ; DNA, Bacterial/genetics ; Flagellin/genetics ; Ixodes/microbiology ; Lyme Disease ; Phylogeny ; Polymorphism, Restriction Fragment Length ; Serotyping
Chemical Substances	Antigens, Bacterial ; Bacterial Outer Membrane Proteins ; DNA, Bacterial ; OspC protein ; flaB1 protein, bacteria ; Flagellin (12777-81-0)
Language	English
Publishing date	2016-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	2212806-2
ISSN	1948-7134 ; 1081-1710
ISSN (online)	1948-7134
ISSN	1081-1710
DOI	10.1111/jvec.12209
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Article: Vaccine against

Kralova, Natalie / Stepanova, Hana / Gebauer, Jan / Norek, Adam / Matiaskova, Katarina / Zouharova, Monika / Nedbalcova, Katerina / Babak, Vladimir / Jarosova, Rea / Makovicky, Peter / Kucharovicova, Ivana / Simek, Bronislav / Plodkova, Hana / Pecka, Tomas / Matiasovic, Jan

Vaccines

2022 Volume 10, Issue 10

Abstract: Streptococcus ... ...

Abstract	Streptococcus suis
Language	English
Publishing date	2022-09-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines10101620
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Article ; Online: Activity of the tick Ixodes ricinus monitored in a suburban park in Brno, Czech Republic, in association with the evaluation of selected repellents.

Žákovská, A / Nejezchlebová, H / Bartoňková, N / Rašovská, T / Kučerová, H / Norek, A / Ovesná, P

Journal of vector ecology : journal of the Society for Vector Ecology

2013 Volume 38, Issue 2, Page(s) 295–300

Abstract: The ever-increasing number of Lyme borreliosis patients led us to consider more effective procedures for disease prevention. The aim of our study was to monitor the annual activity and infectivity of Ixodes ricinus ticks in the Pisárky region, City of ... ...

Abstract	The ever-increasing number of Lyme borreliosis patients led us to consider more effective procedures for disease prevention. The aim of our study was to monitor the annual activity and infectivity of Ixodes ricinus ticks in the Pisárky region, City of Brno, CR, and to test the responses of the locally-captured ticks to selected repellents. The result of regular one-hour-per-week monitoring in 2011 was the collection of ticks that directly reflected the highest number of Lyme disease patients (4,835) detected throughout the period of recording in the Czech Republic. The ticks were examined for spirochaetes by dark field microscopy. The positive samples were identified by PCR analysis, confirming that 76% of these were infected with Borrelia burgdorferi sensu lato. Ticks were most abundant in May and June, with August having the highest risk for spirochaetal infection. Tick activity was statistically correlated with temperature. The moving-object-bioassay was used to study repellent efficiency on the Ixodes ricinus nymphs captured in the above-mentioned suburban park. Five selected commercial repellents based on DEET (N, N-diethyl-3-thylbenzamide) showed statistically different effects on the non-repellent control group.
MeSH term(s)	Animals ; Czech Republic ; Insect Repellents/pharmacology ; Ixodes/drug effects ; Ixodes/genetics ; Ixodes/physiology ; Lyme Disease/prevention & control ; Lyme Disease/transmission ; Temperature
Chemical Substances	Insect Repellents
Language	English
Publishing date	2013-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2212806-2
ISSN	1948-7134 ; 1081-1710
ISSN (online)	1948-7134
ISSN	1081-1710
DOI	10.1111/j.1948-7134.2013.12043.x
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Article: DNA-Based Identification and OspC Serotyping in Cultures of Borrelia burgdorferi s.l. Isolated from Ticks Collected in the Moravia (Czech Republic)

Norek, Adam / Å½Ã¡kovskÃ¡ Alena / Janda LubomÃr

Journal of vector ecology. 2016 June, v. 41, no. 1

2016

Abstract: Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in PisÃ¡rky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three ... ...

Abstract	Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in PisÃ¡rky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii, and B. valaisiana). Three samples from the collection contained more than one genospecies. In the other 15 strains, nucleotide sequences of flaB and ospC were determined. The following phylogenetic analysis assigned 12 isolates to genospecies B. garinii and three to B. afzelii. These isolates were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, and G5 (B. garinii) and A3 and A8 (B. afzelii).
Keywords	Borrelia burgdorferi ; loci ; nucleotide sequences ; phylogeny ; restriction fragment length polymorphism ; ticks ; Czech Republic
Language	English
Dates of publication	2016-06
Size	p. 171-177.
Publishing place	Society for Vector Ecology
Document type	Article
ZDB-ID	2212806-2
ISSN	1948-7134 ; 1081-1710
ISSN (online)	1948-7134
ISSN	1081-1710
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The occurrence of Borrelia burgdorferi sensu lato in certain ectoparasites (Mesostigmata, Siphonaptera) of Apodemus flavicollis and Myodes glareolus in chosen localities in the Czech Republic.

Netušil, Jakub / Žákovská, Alena / Vostal, Karel / Norek, Adam / Stanko, Michal

Acta parasitologica

2013 Volume 58, Issue 3, Page(s) 337–341

Abstract: Ticks represent the primary vectors of several serious diseases, including the Lyme disease caused by Borrelia burgdorferi sensu lato (Bbsl). In this study two dominant ectoparasitic groups of arthropods (Mesostigmata, Siphonaptera) were investigated for ...

Abstract	Ticks represent the primary vectors of several serious diseases, including the Lyme disease caused by Borrelia burgdorferi sensu lato (Bbsl). In this study two dominant ectoparasitic groups of arthropods (Mesostigmata, Siphonaptera) were investigated for the presence of borrelian DNA in order to determine their potential role of vectors (or carriers) of this bacterium. All individuals (235) were collected from wild-living rodents obtained in three localities in the Czech Republic (Bažantula, Baba and Křižovice). The majority of parasites were members of the families Parasitidae and Dermanyssidae (Mesostigmata) and families Hystrichopsyllidae and Ceratophyllidae (Siphonaptera). The rodent host species was almost exclusively the yellow-necked mouse (Apodemus flavicollis). Bbsl was detected by the PCR method in the following ectoparasite species: Euryparasitus emarginatus (1), Eulaelaps stabularis (1), Haemogamassus nidi (1), Laelaps agilis (5), Myonyssus gigas (1) (Mesostigmata) and Ctenophthalmus agyrtes (1), C. solutus (3) (Siphonaptera).
MeSH term(s)	Acari/microbiology ; Animals ; Arthropod Vectors ; Arvicolinae/parasitology ; Borrelia burgdorferi Group/genetics ; Borrelia burgdorferi Group/isolation & purification ; Czech Republic ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Murinae/parasitology ; Polymerase Chain Reaction ; Siphonaptera/microbiology
Chemical Substances	DNA, Bacterial
Language	English
Publishing date	2013-08-29
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1132735-2
ISSN	1896-1851 ; 0065-1478 ; 1230-2821
ISSN (online)	1896-1851
ISSN	0065-1478 ; 1230-2821
DOI	10.2478/s11686-013-0147-5
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Article: Na drodze do terapii genowej mukowiscydozy.

Norek, Aleksandra / Bal, Jerzy

Medycyna wieku rozwojowego

2006 Volume 10, Issue 1 Pt 2, Page(s) 247–274

Abstract: Cystic fibrosis (CF) is the most common recessively inherited lethal disease among the Caucasian population. CF is caused by mutations in the CFTR gene. Although several organs and tracts are affected, severe lung disease is the cause of the most of the ... ...

Title translation	On the way to gene therapy in cystic fibrosis.
Abstract	Cystic fibrosis (CF) is the most common recessively inherited lethal disease among the Caucasian population. CF is caused by mutations in the CFTR gene. Although several organs and tracts are affected, severe lung disease is the cause of the most of the morbidity and mortality in CF individuals. Current treatment is aimed at slowing the inevitable progression of lung disease, rather than halting it, or preventing its onset. The isolation of the gene responsible for CF suggested the feasibility of new therapeutic possibilities based on the CFTR gene transfer to CF patients. At present, somatic CF gene therapy clinical trials, using mostly animals but also CF patients, are being conducted. Gene therapy development is restricted by the lack of the appropriate gene vector systems, which could be successfully used to transfer in vivo and protect the therapeutic gene. This is because of the many extracellular, intracellular and immunological barriers, which protect living organisms against invasion of foreign genetic material. Future improvement in gene therapy depends on the more effective ways of the gene transfer methods, creation animal models of the human diseases and development of strategies involved in the new gene construct formulation, which facilitate to control gene transcription activity.
MeSH term(s)	Cell Nucleus/metabolism ; Cystic Fibrosis/genetics ; Cystic Fibrosis/metabolism ; Cystic Fibrosis/therapy ; Cystic Fibrosis Transmembrane Conductance Regulator/genetics ; Cystic Fibrosis Transmembrane Conductance Regulator/metabolism ; Epithelial Cells/metabolism ; Gene Transfer Techniques ; Genetic Therapy/methods ; Genetic Vectors/metabolism ; Humans ; Lung Diseases/genetics ; Lung Diseases/metabolism ; Lung Diseases/therapy ; Transduction, Genetic ; Treatment Outcome
Chemical Substances	Cystic Fibrosis Transmembrane Conductance Regulator (126880-72-6)
Language	Polish
Publishing date	2006-01
Publishing country	Poland
Document type	English Abstract ; Journal Article ; Review
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Article ; Online: What is the percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae?

Nejedla, Petra / Norek, Adam / Vostal, Karel / Zakovska, Alena

Annals of agricultural and environmental medicine : AAEM

2009 Volume 16, Issue 2, Page(s) 273–276

Abstract: In this study, larvae (1,179 ex.) of mosquito genera Culex were examined for the presence of spirochaetes by Dark Field Microscopy (DFM) at the locality of Blansko (Czech Republic) in of 2004-2008. DFM spirochaete positive samples (25.4 %) were ... ...

Abstract	In this study, larvae (1,179 ex.) of mosquito genera Culex were examined for the presence of spirochaetes by Dark Field Microscopy (DFM) at the locality of Blansko (Czech Republic) in of 2004-2008. DFM spirochaete positive samples (25.4 %) were investigated by nested PCR; only 4 samples were positive for the presence of Borrelia burgdorferi sensu lato, which is 0.3 % of the total examined samples. We can conclude that only a low percentage of pathogenic borreliae are presented in mosquito larvae, while the spirochaete of undefined genera infect larvae in high amounts.
MeSH term(s)	Aedes/microbiology ; Animals ; Borrelia burgdorferi/isolation & purification ; Culex/microbiology ; Larva/microbiology ; Time Factors
Language	English
Publishing date	2009
Publishing country	Poland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1215115-4
ISSN	1898-2263 ; 1232-1966
ISSN (online)	1898-2263
ISSN	1232-1966
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