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Article ; Online: Carbon footprint of low-energy buildings in the United Kingdom: Effects of mitigating technological pathways and decarbonization strategies

Norouzi, Masoud / Haddad, Assed N. / Jiménez, Laureano / Hoseinzadeh, Siamak / Boer, Dieter

Science of the Total Environment. 2023 July, v. 882 p.163490-

2023

Abstract: There is a limited comprehensive analysis of the effectiveness of adopted carbon mitigation strategies for buildings over their life cycle, that are concerned with temporal perspectives of emissions. Accordingly, this paper explores a life cycle ... ...

Abstract	There is a limited comprehensive analysis of the effectiveness of adopted carbon mitigation strategies for buildings over their life cycle, that are concerned with temporal perspectives of emissions. Accordingly, this paper explores a life cycle assessment (LCA) to address the concerns regarding mitigating the carbon footprint of a UK timber-frame low-energy dwelling. In particular, it aims to investigate the potential greenhouse gas (GHG) emission reduction in terms of three different heating and ventilation options, and to analyze the influence of decarbonization of electricity production as well as the technological progress of the waste treatment of timber on the building's environmental performance. Thus, the whole life‑carbon of the building case studies was evaluated for a total of eight investigated prospective scenarios, and they were compared to the LCA results of the baseline scenario, where the existing technology and context remained constant over time. Results show that using a compact heat pump would lead to a significant whole life-cycle emission reduction of the dwelling, by 19 %; while GHG emission savings can be reinforced if the assessed systems are employed simultaneously with grid decarbonization, exhibiting a 25 %-60 % reduction compared to the baseline scenario. Moreover, technological changes in the waste treatments of timber products could substantially reduce the buildings' embodied emissions, representing 3 %-23 %. From these emission-saving measures, the contribution of material efficiency strategies to achieve more embodied carbon savings should be highlighted in future construction practices.
Keywords	carbon ; carbon footprint ; electricity generation ; environment ; environmental performance ; greenhouse gas emissions ; greenhouse gases ; heat ; heat pumps ; life cycle assessment ; waste treatment ; United Kingdom ; ASHRAE ; BECCS ; BIM ; BIPV ; BIPVT ; CCC ; CCS ; CO2 ; CO2eq ; CH4 ; DEFRA ; DB ; EC ; EoL ; EPD ; EPS ; ESL ; FES ; FSC ; FU ; gbXML ; GGBFS ; GHG ; GIA ; GWP ; HVAC ; ISO ; LCA ; LCI ; LCIA ; LETI ; MVHR ; nZEB ; OC ; PCRs ; PEFC ; PV ; RES ; RICS ; RSP ; SAP ; SCOP ; SCMs ; U-value ; UKGBC ; WRAP ; XPS ; Decarbonization strategy ; Timber ; Low-energy building
Language	English
Dates of publication	2023-07
Publishing place	Elsevier B.V.
Document type	Article ; Online
Note	Pre-press version ; Use and reproduction
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2023.163490
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: High-Efficiency Protection of Linear DNA in Cell-Free Extracts from

Norouzi, Masoud / Panfilov, Sabina / Pardee, Keith

ACS synthetic biology

2021 Volume 10, Issue 7, Page(s) 1615–1624

Abstract: The field of cell-free synthetic biology is an emerging branch of engineered biology that allows for rapid prototyping of biological designs and, in its own right, is becoming a venue for ... ...

Abstract	The field of cell-free synthetic biology is an emerging branch of engineered biology that allows for rapid prototyping of biological designs and, in its own right, is becoming a venue for the
MeSH term(s)	Cell-Free Nucleic Acids/chemistry ; Cell-Free Nucleic Acids/genetics ; Escherichia coli/genetics ; Nucleic Acid Conformation ; Proof of Concept Study ; Synthetic Biology/methods ; Vibrio/genetics
Chemical Substances	Cell-Free Nucleic Acids
Language	English
Publishing date	2021-06-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2161-5063
ISSN (online)	2161-5063
DOI	10.1021/acssynbio.1c00110
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Article ; Online: Carbon footprint of low-energy buildings in the United Kingdom: Effects of mitigating technological pathways and decarbonization strategies.

Norouzi, Masoud / Haddad, Assed N / Jiménez, Laureano / Hoseinzadeh, Siamak / Boer, Dieter

The Science of the total environment

2023 Volume 882, Page(s) 163490

Abstract	There is a limited comprehensive analysis of the effectiveness of adopted carbon mitigation strategies for buildings over their life cycle, that are concerned with temporal perspectives of emissions. Accordingly, this paper explores a life cycle assessment (LCA) to address the concerns regarding mitigating the carbon footprint of a UK timber-frame low-energy dwelling. In particular, it aims to investigate the potential greenhouse gas (GHG) emission reduction in terms of three different heating and ventilation options, and to analyze the influence of decarbonization of electricity production as well as the technological progress of the waste treatment of timber on the building's environmental performance. Thus, the whole life‑carbon of the building case studies was evaluated for a total of eight investigated prospective scenarios, and they were compared to the LCA results of the baseline scenario, where the existing technology and context remained constant over time. Results show that using a compact heat pump would lead to a significant whole life-cycle emission reduction of the dwelling, by 19 %; while GHG emission savings can be reinforced if the assessed systems are employed simultaneously with grid decarbonization, exhibiting a 25 %-60 % reduction compared to the baseline scenario. Moreover, technological changes in the waste treatments of timber products could substantially reduce the buildings' embodied emissions, representing 3 %-23 %. From these emission-saving measures, the contribution of material efficiency strategies to achieve more embodied carbon savings should be highlighted in future construction practices.
Language	English
Publishing date	2023-04-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2023.163490
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Article: Impact of Vimentin on Regulation of Cell Signaling and Matrix Remodeling.

Ostrowska-Podhorodecka, Zofia / Ding, Isabel / Norouzi, Masoud / McCulloch, Christopher A

Frontiers in cell and developmental biology

2022 Volume 10, Page(s) 869069

Abstract: Vimentin expression contributes to cellular mechanoprotection and is a widely recognized marker of fibroblasts and of epithelial-mesenchymal transition. But it is not understood how vimentin affects signaling that controls cell migration and ... ...

Abstract	Vimentin expression contributes to cellular mechanoprotection and is a widely recognized marker of fibroblasts and of epithelial-mesenchymal transition. But it is not understood how vimentin affects signaling that controls cell migration and extracellular matrix (ECM) remodeling. Recent data indicate that vimentin controls collagen deposition and ECM structure by regulating contractile force application to the ECM and through post-transcriptional regulation of ECM related genes. Binding of cells to the ECM promotes the association of vimentin with cytoplasmic domains of adhesion receptors such as integrins. After initial adhesion, cell-generated, myosin-dependent forces and signals that impact vimentin structure can affect cell migration. Post-translational modifications of vimentin determine its adaptor functions, including binding to cell adhesion proteins like paxillin and talin. Accordingly, vimentin regulates the growth, maturation and adhesive strength of integrin-dependent adhesions, which enables cells to tune their attachment to collagen, regulate the formation of cell extensions and control cell migration through connective tissues. Thus, vimentin tunes signaling cascades that regulate cell migration and ECM remodeling. Here we consider how specific properties of vimentin serve to control cell attachment to the underlying ECM and to regulate mesenchymal cell migration and remodeling of the ECM by resident fibroblasts.
Language	English
Publishing date	2022-03-11
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2022.869069
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Article ; Online: Vimentin-mediated myosin 10 aggregation at tips of cell extensions drives MT1-MMP-dependent collagen degradation in colorectal cancer.

Ostrowska-Podhorodecka, Zofia / Ali, Aiman / Norouzi, Masoud / Ding, Isabel / Abbasi, Sevil / Arora, Pamma D / Wong, Timothy H F / Magalhaes, Marco / McCulloch, Christopher A

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

2024 Volume 37, Issue 8, Page(s) e23097

Abstract: Colorectal cancer (CRC) is a high prevalence adenocarcinoma with progressive increases in metastasis-related mortality, but the mechanisms governing the extracellular matrix (ECM) degradation important for metastasis in CRC are not well-defined. We ... ...

Abstract	Colorectal cancer (CRC) is a high prevalence adenocarcinoma with progressive increases in metastasis-related mortality, but the mechanisms governing the extracellular matrix (ECM) degradation important for metastasis in CRC are not well-defined. We investigated a functional relationship between vimentin (Vim) and myosin 10 (Myo10), and whether this relationship is associated with cancer progression. We tested the hypothesis that Vim regulates the aggregation of Myo10 at the tips of cell extensions, which increases membrane-type 1 matrix metalloproteinase (MT1-MMP)-associated local collagen proteolysis and ECM degradation. Analysis of CRC samples revealed colocalization of Vim with Myo10 and MT1-MMP in cell extensions adjacent to sites of collagen degradation, suggesting an association with local cell invasion. We analyzed cultured CRC cells and fibroblasts and found that Vim accelerates aggregation of Myo10 at cell tips, which increases the cell extension rate. Vim stabilizes the interaction of Myo10 with MT1-MMP, which in turn increases collagenolysis. Vim depletion reduced the aggregation of Myo10 at the cell extension tips and MT1-MMP-dependent collagenolysis. We propose that Vim interacts with Myo10, which in turn associates with MT1-MMP to facilitate the transport of these molecules to the termini of cell extensions and there enhance cancer invasion of soft connective tissues.
MeSH term(s)	Humans ; Matrix Metalloproteinase 14/metabolism ; Vimentin/metabolism ; Collagen ; Myosins ; Colorectal Neoplasms
Chemical Substances	Matrix Metalloproteinase 14 (EC 3.4.24.80) ; Vimentin ; Collagen (9007-34-5) ; Myosins (EC 3.6.4.1)
Language	English
Publishing date	2024-01-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639186-2
ISSN	1530-6860 ; 0892-6638
ISSN (online)	1530-6860
ISSN	0892-6638
DOI	10.1096/fj.202300672R
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Article ; Online: Adaptive, diverse and de-centralized diagnostics are key to the future of outbreak response.

Matthews, Quinn / da Silva, Severino Jefferson Ribeiro / Norouzi, Masoud / Pena, Lindomar José / Pardee, Keith

BMC biology

2020 Volume 18, Issue 1, Page(s) 153

Abstract: The global spread of SARS-CoV-2 has shaken our health care and economic systems, prompting re-evaluation of long-held views on how best to deliver care. This is especially the case for our global diagnostic strategy. While current laboratory-based ... ...

Abstract	The global spread of SARS-CoV-2 has shaken our health care and economic systems, prompting re-evaluation of long-held views on how best to deliver care. This is especially the case for our global diagnostic strategy. While current laboratory-based centralized RT-qPCR will continue to serve as a gold standard diagnostic into the foreseeable future, the shortcomings of our dependence on this method have been laid bare. It is now clear that a robust diagnostics pandemic response strategy, like any disaster planning, must include adaptive, diverse and de-centralized solutions. Here we look at how the COVID-19 pandemic, and previous outbreaks, have set the stage for a new innovative phase in diagnostics and a re-thinking of pandemic preparedness.
MeSH term(s)	Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Coronavirus Infections/epidemiology ; Disease Outbreaks ; Humans ; Mass Screening ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/epidemiology ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2
Keywords	covid19
Language	English
Publishing date	2020-10-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1741-7007
ISSN (online)	1741-7007
DOI	10.1186/s12915-020-00891-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Cell-Free Dot Blot: an Ultra-Low-Cost and Practical Immunoassay Platform for Detection of Anti-SARS-CoV-2 Antibodies in Human and Animal Sera.

Norouzi, Masoud / Truong, Thang / Jaenes, Katariina / Warner, Bryce M / Vendramelli, Robert / Tierney, Kevin / Kobasa, Darwyn / Tailor, Nikesh / Plant, Pamela / Dos Santos, Claudia / Babiuk, Shawn / Ambagala, Aruna / Pardee, Keith

Microbiology spectrum

2023 , Page(s) e0245722

Abstract: Since its emergence in late 2019, the coronavirus disease 2019 (COVID-19) pandemic has caused severe disruption to key aspects of human life globally and highlighted the need for timely, adaptive, and accessible pandemic response strategies. Here, we ... ...

Abstract	Since its emergence in late 2019, the coronavirus disease 2019 (COVID-19) pandemic has caused severe disruption to key aspects of human life globally and highlighted the need for timely, adaptive, and accessible pandemic response strategies. Here, we introduce the cell-free dot blot (CFDB) method, a practical and ultra-low-cost immune diagnostic platform capable of rapid response and mass immunity screening for the current and future pandemics. Similar in mechanism to the widely used enzyme-linked immunosorbent assays (ELISAs), our method is novel and advantageous in that (i) it uses linear DNA to produce the target viral antigen fused to a SpyTag peptide in a cell-free expression system without the need for traditional cloning and antigen purification, (ii) it uses SpyCatcher2-Apex2, an Escherichia coli-produced peroxidase conjugate as a universal secondary detection reagent, obviating the need for commercial or sophisticated enzyme conjugates, and (iii) sera are spotted directly on a nitrocellulose membrane, enabling a simple "dipping" mechanism for downstream incubation and washing steps, as opposed to individual processing of wells in a multiwell plate. To demonstrate the utility of our method, we performed CFDB to detect anti-severe acute respiratory syndrome coronavirus 2 nucleocapsid protein antibodies in precharacterized human sera (23 negative and 36 positive for COVID-19) and hamster sera (16 negative and 36 positive for COVID-19), including independent testing at a collaborating laboratory, and we show assay performance comparable to that of conventional ELISAs. At a similar capacity to 96-well plate ELISA kits, one CFDB assay costs only ~$3 USD. We believe that CFDB can become a valuable pandemic response tool for adaptive and accessible sero-surveillance in human and animal populations.
Language	English
Publishing date	2023-01-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.02457-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Application of mRNA Arrays for the Production of mCherry Reporter-Protein Arrays for Quantitative Gene Expression Analysis.

Norouzi, Masoud / Pickford, Andrew R / Butt, Louise E / Vincent, Helen A / Callaghan, Anastasia J

ACS synthetic biology

2019 Volume 8, Issue 2, Page(s) 207–215

Abstract: The development of programmable regulators that precisely and predictably control gene expression is a major goal of synthetic biology. Consequently, rapid high-throughput biochemical methods capable of quantitatively analyzing all components of gene ... ...

Abstract	The development of programmable regulators that precisely and predictably control gene expression is a major goal of synthetic biology. Consequently, rapid high-throughput biochemical methods capable of quantitatively analyzing all components of gene expression would be of value in the characterization and optimization of regulator performance. In this study we demonstrate a novel application of RNA arrays, involving the production of reporter-protein arrays, to gene expression analysis. This method enables simultaneous quantification of both the transcription and post-transcription/translation components of gene expression, and it also allows the assessment of the orthogonality of multiple regulators. We use our method to directly compare the performance of a series of previously characterized synthetic post-transcriptional riboregulators, thus demonstrating its utility in the development of synthetic regulatory modules and evaluation of gene expression regulation in general.
MeSH term(s)	Nucleic Acid Conformation ; Nucleic Acid Hybridization/methods ; Protein Array Analysis/methods ; RNA, Messenger/metabolism ; Synthetic Biology
Chemical Substances	RNA, Messenger
Language	English
Publishing date	2019-01-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2161-5063
ISSN (online)	2161-5063
DOI	10.1021/acssynbio.8b00266
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics.

Jaenes, Katariina / Ribeiro da Silva, Severino Jefferson / Vigar, Justin R J / Wu, Kaiyue / Norouzi, Masoud / Bayat, Pouriya / Karlikow, Margot / Cicek, Seray / Guo, Yuxiu / Green, Alexander A / Pena, Lindomar / Pardee, Keith

Journal of visualized experiments : JoVE

2022 , Issue 184

Abstract: Access to low-burden molecular diagnostics that can be deployed into the community for testing is increasingly important and has meaningful wider implications for the well-being of societies and economic stability. Recent years have seen several new ... ...

Abstract	Access to low-burden molecular diagnostics that can be deployed into the community for testing is increasingly important and has meaningful wider implications for the well-being of societies and economic stability. Recent years have seen several new isothermal diagnostic modalities emerge to meet the need for rapid, low-cost molecular diagnostics. We have contributed to this effort through the development and patient validation of toehold switch-based diagnostics, including diagnostics for the mosquito-borne Zika and chikungunya viruses, which provided performance comparable to gold-standard reverse transcription-quantitative polymerase chain reaction (RT-qPCR) based assays. These diagnostics are inexpensive to develop and manufacture, and they have the potential to provide diagnostic capacity to low-resource environments. Here the protocol provides all the steps necessary for the development of a switch-based assay for Zika virus detection. The article takes readers through the stepwise diagnostic development process. First, genomic sequences of Zika virus serve as inputs for the computational design of candidate switches using open-source software. Next, the assembly of the sensors for empirical screening with synthetic RNA sequences and optimization of diagnostic sensitivity is shown. Once complete, validation is performed with patient samples in parallel with RT-qPCR, and a purpose-built optical reader, PLUM. This work provides a technical roadmap to researchers for the development of low-cost toehold switch-based sensors for applications in human health, agriculture, and environmental monitoring.
MeSH term(s)	Animals ; Chikungunya virus ; Humans ; RNA, Viral/analysis ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/methods ; Zika Virus/genetics ; Zika Virus Infection/diagnosis
Chemical Substances	RNA, Viral
Language	English
Publishing date	2022-06-17
Publishing country	United States
Document type	Journal Article ; Video-Audio Media ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/63223
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Adaptive, diverse and de-centralized diagnostics are key to the future of outbreak response

Matthews, Quinn / da Silva, Severino Jefferson Ribeiro / Norouzi, Masoud / Pena, Lindomar José / Pardee, Keith

BMC Biol

Abstract	The global spread of SARS-CoV-2 has shaken our health care and economic systems, prompting re-evaluation of long-held views on how best to deliver care. This is especially the case for our global diagnostic strategy. While current laboratory-based centralized RT-qPCR will continue to serve as a gold standard diagnostic into the foreseeable future, the shortcomings of our dependence on this method have been laid bare. It is now clear that a robust diagnostics pandemic response strategy, like any disaster planning, must include adaptive, diverse and de-centralized solutions. Here we look at how the COVID-19 pandemic, and previous outbreaks, have set the stage for a new innovative phase in diagnostics and a re-thinking of pandemic preparedness.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #895003
Database	COVID19

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