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  1. Article ; Online: CSF-contacting neurons regulate locomotion by relaying mechanical stimuli to spinal circuits.

    Böhm, Urs Lucas / Prendergast, Andrew / Djenoune, Lydia / Nunes Figueiredo, Sophie / Gomez, Johanna / Stokes, Caleb / Kaiser, Sonya / Suster, Maximilliano / Kawakami, Koichi / Charpentier, Marine / Concordet, Jean-Paul / Rio, Jean-Paul / Del Bene, Filippo / Wyart, Claire

    Nature communications

    2016  Volume 7, Page(s) 10866

    Abstract: Throughout vertebrates, cerebrospinal fluid-contacting neurons (CSF-cNs) are ciliated cells surrounding the central canal in the ventral spinal cord. Their contribution to modulate locomotion remains undetermined. Recently, we have shown CSF-cNs modulate ...

    Abstract Throughout vertebrates, cerebrospinal fluid-contacting neurons (CSF-cNs) are ciliated cells surrounding the central canal in the ventral spinal cord. Their contribution to modulate locomotion remains undetermined. Recently, we have shown CSF-cNs modulate locomotion by directly projecting onto the locomotor central pattern generators (CPGs), but the sensory modality these cells convey to spinal circuits and their relevance to innate locomotion remain elusive. Here, we demonstrate in vivo that CSF-cNs form an intraspinal mechanosensory organ that detects spinal bending. By performing calcium imaging in moving animals, we show that CSF-cNs respond to both passive and active bending of the spinal cord. In mutants for the channel Pkd2l1, CSF-cNs lose their response to bending and animals show a selective reduction of tail beat frequency, confirming the central role of this feedback loop for optimizing locomotion. Altogether, our study reveals that CSF-cNs constitute a mechanosensory organ operating during locomotion to modulate spinal CPGs.
    MeSH term(s) Animals ; Biomechanical Phenomena ; Cell Movement ; Cerebrospinal Fluid/cytology ; Cerebrospinal Fluid/metabolism ; Female ; Male ; Mechanoreceptors/cytology ; Mechanoreceptors/metabolism ; Neurons/cytology ; Neurons/metabolism ; Spinal Cord/chemistry ; Spinal Cord/cytology ; Spinal Cord/metabolism ; Transient Receptor Potential Channels/genetics ; Transient Receptor Potential Channels/metabolism ; Zebrafish/genetics ; Zebrafish/metabolism ; Zebrafish Proteins/genetics ; Zebrafish Proteins/metabolism
    Chemical Substances Transient Receptor Potential Channels ; Zebrafish Proteins
    Language English
    Publishing date 2016-03-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/ncomms10866
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Investigation of spinal cerebrospinal fluid-contacting neurons expressing PKD2L1: evidence for a conserved system from fish to primates.

    Djenoune, Lydia / Khabou, Hanen / Joubert, Fanny / Quan, Feng B / Nunes Figueiredo, Sophie / Bodineau, Laurence / Del Bene, Filippo / Burcklé, Céline / Tostivint, Hervé / Wyart, Claire

    Frontiers in neuroanatomy

    2014  Volume 8, Page(s) 26

    Abstract: Over 90 years ago, Kolmer and Agduhr identified spinal cerebrospinal fluid-contacting neurons (CSF-cNs) based on their morphology and location within the spinal cord. In more than 200 vertebrate species, they observed ciliated neurons around the central ... ...

    Abstract Over 90 years ago, Kolmer and Agduhr identified spinal cerebrospinal fluid-contacting neurons (CSF-cNs) based on their morphology and location within the spinal cord. In more than 200 vertebrate species, they observed ciliated neurons around the central canal that extended a brush of microvilli into the cerebrospinal fluid (CSF). Although their morphology is suggestive of a primitive sensory cell, their function within the vertebrate spinal cord remains unknown. The identification of specific molecular markers for these neurons in vertebrates would benefit the investigation of their physiological roles. PKD2L1, a transient receptor potential channel that could play a role as a sensory receptor, has been found in cells contacting the central canal in mouse. In this study, we demonstrate that PKD2L1 is a specific marker for CSF-cNs in the spinal cord of mouse (Mus musculus), macaque (Macaca fascicularis) and zebrafish (Danio rerio). In these species, the somata of spinal PKD2L1(+) CSF-cNs were located below or within the ependymal layer and extended an apical bulbous extension into the central canal. We found GABAergic PKD2L1-expressing CSF-cNs in all three species. We took advantage of the zebrafish embryo for its transparency and rapid development to identify the progenitor domains from which pkd2l1 (+) CSF-cNs originate. pkd2l1 (+) CSF-cNs were all GABAergic and organized in two rows-one ventral and one dorsal to the central canal. Their location and marker expression is consistent with previously described Kolmer-Agduhr cells. Accordingly, pkd2l1 (+) CSF-cNs were derived from the progenitor domains p3 and pMN defined by the expression of nkx2.2a and olig2 transcription factors, respectively. Altogether our results suggest that a system of CSF-cNs expressing the PKD2L1 channel is conserved in the spinal cord across bony vertebrate species.
    Language English
    Publishing date 2014-05-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2452969-2
    ISSN 1662-5129
    ISSN 1662-5129
    DOI 10.3389/fnana.2014.00026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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