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Article ; Online: The free energy landscape of the oncogene protein E7 of human papillomavirus type 16 reveals a complex interplay between ordered and disordered regions

Predrag Kukic / Giuseppe Mattia Lo Piccolo / Marcela O. Nogueira / Dmitri I. Svergun / Michele Vendruscolo / Isabella C. Felli / Roberta Pierattelli

Scientific Reports, Vol 9, Iss 1, Pp 1-

2019 Volume 12

Abstract: Abstract When present, structural disorder makes it very challenging to characterise the conformational properties of proteins. This is particularly the case of proteins, such as the oncogene protein E7 of human papillomavirus type 16, which contain both ...

Abstract	Abstract When present, structural disorder makes it very challenging to characterise the conformational properties of proteins. This is particularly the case of proteins, such as the oncogene protein E7 of human papillomavirus type 16, which contain both ordered and disordered domains, and that can populate monomeric and oligomeric states under physiological conditions. Nuclear magnetic resonance (NMR) spectroscopy is emerging as a powerful method to study these complex systems, most notably in combination with molecular dynamics simulations. Here we use NMR chemical shifts and residual dipolar couplings as structural restraints in replica-averaged molecular dynamics simulations to determine the free energy landscape of E7. This landscape reveals a complex interplay between a folded but highly dynamical C-terminal domain and a disordered N-terminal domain that forms transient secondary and tertiary structures, as well as an equilibrium between a high-populated (98%) dimeric state and a low-populated (2%) monomeric state. These results provide compelling evidence of the complex conformational heterogeneity associated with the behaviour and interactions of this disordered protein associated with disease.
Keywords	Medicine ; R ; Science ; Q
Subject code	612
Language	English
Publishing date	2019-04-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Superação da dormência e o uso de fungicida em diásporos de teca

Elza C. Coimbra / Gisele H. Vazquez / Thaissa O. Nogueira

Revista Brasileira de Engenharia Agrícola e Ambiental - Agriambi, Vol 18, Iss 12, Pp 1281-

2014 Volume 1286

Abstract: Objetivou-se, nesta pesquisa, testar métodos de superação da dormência além de avaliar a eficiência do uso de fungicida no armazenamento de diásporos de teca que já sofreram tratamentos pré-germinativos. O delineamento experimental foi o inteiramente ... ...

Abstract	Objetivou-se, nesta pesquisa, testar métodos de superação da dormência além de avaliar a eficiência do uso de fungicida no armazenamento de diásporos de teca que já sofreram tratamentos pré-germinativos. O delineamento experimental foi o inteiramente casualizado em esquema fatorial 7 x 2 x 2 (sete métodos de superação; com e sem fungicida e dois tempos de avaliação, zero e após 120 dias). Os métodos utilizados foram: imersão em água corrente (24 h) e em água quente (85-90 ºC por 8 min); escarificação com lixa + imersão em água parada (48 h); escarificação com ácido sulfúrico por 15 min + água corrente (72 h); aquecimento em estufa a 80 ºC (4 h por 3 dias) + imersão em água parada (12 h); solarização por 48 h + imersão em água parada (12 h) além da testemunha. Os métodos de superação com aquecimento a seco dos diásporos de teca são capazes de abreviar, aumentar e uniformizar a germinação sendo o uso do solarizador uma boa opção por não consumir energia e ser de baixo custo. O uso do fungicida Fludioxonil + Metalaxyl-M e o armazenamento de diásporos de teca que já sofreram tratamento de quebra da dormência, não são recomendados.
Keywords	Tectona grandis ; escarificação ; solarizador ; Agriculture (General) ; S1-972
Language	English
Publishing date	2014-12-01T00:00:00Z
Publisher	Universidade Federal de Campina Grande
Document type	Article ; Online
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Article ; Online: Exposure to anti-smoking advertising across six European countries, findings from the international tobacco control six european countries (ITC-E6) survey of the EUREST-PLUS study

Sarah O Nogueira / Sara C Hitchman / Ann McNeill / on behalf of the EUREST-PLUS consortium

Tobacco Prevention and Cessation, Vol 4, Iss Supplement (2018)

2018

Abstract: Introduction Exposure to anti-smoking advertising and its effects differ across countries. This study examines: 1) reported exposure to anti-smoking advertising on traditional mass media and internet/social media; 2) the relationship between exposure to ... ...

Abstract	Introduction Exposure to anti-smoking advertising and its effects differ across countries. This study examines: 1) reported exposure to anti-smoking advertising on traditional mass media and internet/social media; 2) the relationship between exposure to anti-smoking advertising and two other variables: knowledge of the harms of smoking/SHS and quit attempts in six European countries. Methods Data come from the first wave of the International Tobacco Control Policy Evaluation Project 6 European Country Survey (ITC 6E) (Germany [DE], Greece [GR], Hungary [HU], Poland [PL], Romania [RO] and Spain [ES]) carried out between June 2016 and September 2016 (n=5805). Key measures included whether in the last six months participants had noticed anti-smoking advertising, their knowledge of 13 adverse smoking/SHS health effects and if they have made at least one quit attempt in the last 12 months. Results RO and PL participants had significant higher odds of reporting exposure to anti-smoking advertising than DE, GR, HU and ES participants in 4 of the 6 of the media venues. Reported exposure to anti-smoking advertising was not associated with higher health knowledge in the six countries. There was a significant and positive association between exposure to anti-smoking advertising and quit attempts in the last 12 months. Conclusions Participants in countries where an anti-smoking advertising campaign happened in the last 2 years, had the highest odds to report exposure to anti-smoking advertising. Participants exposed to anti-smoking advertising were more likely to make a quit attempt than those not exposed. However, exposure to anti-smoking advertising was not associated with health knowledge. Funding Travel expenses for the conference will be funded by La Caixa Fellowship programme through the European Union’s Horizon 2020 research and innovation programme under the Marie Sklowdowska-Curie grant agreement. This work was supported by a grant from the European Commission (Horizon2020 HCO-6-2015; EUREST-PLUS: 681109)
Keywords	tobacco control ; Europe ; Public aspects of medicine ; RA1-1270
Subject code	300
Language	English
Publishing date	2018-06-01T00:00:00Z
Publisher	European Publishing
Document type	Article ; Online
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Article ; Online: Maternal physical activity prevents the overexpression of hypoxia-inducible factor 1-α and cardiorespiratory dysfunction in protein malnourished rats

Viviane O. Nogueira / Luana D. S. Andrade / Reginaldo L. Rocha-Júnior / Palloma E. D. Melo / Elisama Helvécio / Danilo A. F. Fontes / Tatiany P. Romão / Carol G. Leandro / João H. Costa-Silva

Scientific Reports, Vol 9, Iss 1, Pp 1-

2019 Volume 12

Abstract: Abstract Maternal physical activity attenuates cardiorespiratory dysfunctions and transcriptional alterations presented by the carotid body (CB) of rats. Rats performed physical activity and were classified as inactive/active. During gestation and ... ...

Abstract	Abstract Maternal physical activity attenuates cardiorespiratory dysfunctions and transcriptional alterations presented by the carotid body (CB) of rats. Rats performed physical activity and were classified as inactive/active. During gestation and lactation, mothers received either normoprotein (NP-17% protein) or low-protein diet (LP-8% protein). In offspring, biochemical serum levels, respiratory parameters, cardiovascular parameters and the mRNA expression of hypoxia-inducible factor 1-alpha (HIF-1α), tyrosine hydroxylase (TH) and purinergic receptors were evaluate. LP-inactive pups presented lower RF from 1st to 14th days old, and higher RF at 30 days than did NP-inactive and NP-active pups. LP-inactive pups presented with reduced serum protein, albumin, cholesterol and triglycerides levels and an increased fasting glucose level compared to those of NP-inactive and NP-active groups. LP and LP-inactive animals showed an increase in the cardiac variability at the Low-Frequency bands, suggesting a major influence of sympathetic nervous activity. In mRNA analyses, LP-inactive animals showed increased HIF-1α expression and similar expression of TH and purinergic receptors in the CB compared to those of NP groups. All these changes observed in LP-inactive pups were reversed in the pups of active mothers (LP-active). Maternal physical activity is able to attenuate the metabolic, cardiorespiratory and HIF-1α transcription changes induced by protein malnutrition.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2019-10-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Molecular epidemiology of Mycobacterium tuberculosis strains from prison populations in Santa Catarina, Southern Brazil

Medeiros, Taiane Freitas / Christiane LourenÃ§o Nogueira / Darcita BÃ¼erger Rovaris / Eduardo VenÃ¢ncio Alves / Mara Cristina Scheffer / Maria Luiza Bazzo / Nalin Rastogi / Rodrigo Ivan Prim / Thierry Zozio

Infection, genetics, and evolution. 2018 Mar., v. 58

2018

Abstract: The Tuberculosis (TB) notification rates are 5 to 81 times higher in prisons worldwide when compared to the general population. The state of Santa Catarina (SC) has few epidemiological data regarding TB in prisons. The aim of this study was to evaluate ... ...

Abstract	The Tuberculosis (TB) notification rates are 5 to 81 times higher in prisons worldwide when compared to the general population. The state of Santa Catarina (SC) has few epidemiological data regarding TB in prisons. The aim of this study was to evaluate the molecular epidemiology of circulating strains in prisons of SC. The study comprised 95 clinical samples from six prisons. Among the cases included, all subjects were male, predominantly caucasians, and young adults, with low education level. The positive smear in the TB diagnosis comprised 62.0% of cases. About 50% of subjects had some condition associated with TB. The Spoligotyping results showed that the most frequent lineages were LAM (50.7%), T (22.2%) and S (11.6%). The 12-loci MIRU generated 62 different genotypes. The MSTs showed evolutionary relationships between Mycobacterium tuberculosis spoligotypes from SC and evolutionary relationships between the prison isolates and studied parameters. This first study on TB in prison units of SC highlighted the predominance of SIT216/LAM5, and SIT34/S. Interestingly, his profile was found to be different from that observed in a previous study performed with the state's general population. This data shows the need for continued surveillance of episodes of TB occurring among prison inmates in an emerging country like Brazil.
Keywords	correctional institutions ; developing countries ; genotype ; low educational status ; males ; molecular epidemiology ; monitoring ; Mycobacterium tuberculosis ; prison inmates ; tuberculosis ; young adults ; Brazil
Language	English
Dates of publication	2018-03
Size	p. 34-39.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2037068-4
ISSN	1567-1348
ISSN	1567-1348
DOI	10.1016/j.meegid.2017.12.010
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Induction and growth pattern of callus from Piper permucronatum leaves

M.R.A. SANTOS / M.C.M. GUIMARÃES / E.S. PAZ / G.M.O. MAGALHÃES / C.A. SOUZA / C.V. SMOZINSKI / W.O. NOGUEIRA

Revista Brasileira de Plantas Medicinais , Vol 18, Iss 1, Pp 142-

2016 Volume 148

Abstract: ABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and ... ...

Abstract	ABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.
Keywords	Piperaceae ; reguladores de crescimento ; calogênese ; Pharmacy and materia medica ; RS1-441 ; Medicine ; R ; Botany ; QK1-989 ; Science ; Q
Subject code	580
Language	Portuguese
Publishing date	2016-03-01T00:00:00Z
Publisher	UNESP
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Maternal low-protein diet induces changes in the cardiovascular autonomic modulation in male rat offspring

Barros, M.A.V / A.G. Wanderley / J.H. Costa-Silva / J.L. De Brito Alves / V.O. Nogueira

Nutrition, metabolism, and cardiovascular diseases. 2015 Jan., v. 25, no. 1

2015

Abstract: Maternal undernutrition induces development of the arterial hypertension. We investigated the effects of a maternal low-protein diet on cardiovascular autonomic control in the offspring.Male Wistar rats were divided into two groups according to the diets ...

Abstract	Maternal undernutrition induces development of the arterial hypertension. We investigated the effects of a maternal low-protein diet on cardiovascular autonomic control in the offspring.Male Wistar rats were divided into two groups according to the diets of their mothers during gestation and lactation: the control (normal protein, NP, 17% casein; n = 14) and low-protein (LP, 8% casein; n = 14) groups. Direct measurements of arterial pressure (AP) were recorded from wakeful 90-day-old male offspring. The LP offspring presented higher mean AP than did the NP rats (NP: 93 ± 4 vs. LP: 113 ± 2 mmHg; p < 0.05), whereas the heart rate (HR) was similar in the two groups. In the spectral analysis, the LP group showed higher power at low (NP: 1.98 ± 0.25 vs. LP: 3.7 ± 0.3 mmHg2; p < 0.05) and high (NP: 1.28 ± 0.18 vs. LP: 2.13 ± 0.42 mmHg2; p < 0.05) frequencies of systolic arterial pressure (SAP). In the pulse interval, the LP group presented an increase in the LF/HF ratio (NP: 0.32 vs. LP: 0.56; p < 0.05). After propranolol (4 mg/kg, intravenous (iv)), the bradycardia was higher in the LP group (NP: −36 ± 8 vs. LP: −94 ± 12 bpm; p < 0.05), after methylatropine (2 mg/kg, iv), the tachycardia was similar to NP group. After administration of the ganglionic blocker (hexamethonium; 25 mg/kg, iv), the LP animals showed larger delta variation in the AP (NP: −33.7 ± 5 vs. LP: −53.6 ± 4 mmHg; p < 0.05).The rats subjected to protein malnutrition presented an increase in the cardiovascular sympathetic tone, which contributed to the elevated AP observed in these animals.
Keywords	casein ; heart rate ; hypertension ; intravenous injection ; kwashiorkor ; lactation ; low protein diet ; males ; metabolism ; mothers ; pregnancy ; progeny ; propranolol ; rats ; spectral analysis ; systolic blood pressure ; tachycardia
Language	English
Dates of publication	2015-01
Size	p. 123-130.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1067704-5
ISSN	0939-4753
ISSN	0939-4753
DOI	10.1016/j.numecd.2014.07.011
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A VP3/VP1 gene polymerase chain reaction assay for detection of chicken anemia virus in broiler samples Teste de reação em cadeia de polimerase dos genes VP3 e VP1 para detecção do vírus da anemia das galinhas em amostras de campo

E.O. Nogueira / L. Brentano / A.J.P. Ferreira

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Vol 57, Pp 131-

2005 Volume 140

Abstract: A PCR assay was designed for amplification of the highly conserved VP3 gene and a 5' region of the VP1 gene, for the diagnosis of CAV in organ samples of broiler flocks suspected of chicken infectious anemia. A comparison of the VP3/VP1 PCR with in vivo ... ...

Abstract	A PCR assay was designed for amplification of the highly conserved VP3 gene and a 5' region of the VP1 gene, for the diagnosis of CAV in organ samples of broiler flocks suspected of chicken infectious anemia. A comparison of the VP3/VP1 PCR with in vivo virus isolation revealed 100% agreement of the results, with 13 positive and 3 negative samples in both assays, indicating that the VP3/VP1 PCR is a specific diagnostic method. Tissues from additional 24 broiler chicken flocks, with CAV-like lesions and clinical history were then tested only by the VP3/VP1 PCR and a reference PCR with published primers for the VP1 gene. Nineteen samples resulted positive and one negative in both PCR, while another 4 samples were positive only in the VP3/VP1 PCR. These results indicate that the VP3/VP1 PCR is a sensitive, specific diagnostic test, suitable as an alternative to the expensive and time consuming in vivo virus isolation method, specially considering the difficult diagnosis of CAV strains not readily adaptable to MSB-1 cell culture. Desenvolveu-se uma reação em cadeia de polimerase (PCR) para amplificação do altamente conservado gene VP3 e da região 5' do gene VP1, para o diagnóstico do vírus da anemia das galinhas (CAV), diretamente em amostras de campo de órgãos de frangos de corte com suspeita clínica da doença. A comparação entre o PCR VP3/VP1 com isolamento viral in vivo indicou 100% de concordância dos resultados, com 13 amostras positivas e três negativas em ambos os testes. Órgãos de outros 24 lotes de frangos com lesões e história clínica compatível com CAV foram testados com o PCR VP3/VP1 e com um PCR de referência com primers conhecidos para o gene VP1. Dezenove amostras resultaram positivas e uma negativa em ambos os PCR e quatro foram positivas apenas no PCR VP3/VP1. Estes resultados indicam que o PCR VP3/VP1 é um teste de diagnóstico sensível e específico, aplicável como alternativa ao método caro e demorado de isolamento viral in vivo e especialmente considerando-se amostras do CAV não adaptáveis a cultivos de células MSB-1.
Keywords	frango de corte ; vírus da anemia das galinhas ; CAV ; PCR ; teste de diagnóstico ; chicken ; chicken anemia virus ; diagnostic test ; Zoology ; QL1-991 ; Science ; Q ; DOAJ:Zoology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Animal culture ; SF1-1100 ; Agriculture ; S ; DOAJ:Animal Sciences ; DOAJ:Agriculture and Food Sciences
Language	English
Publishing date	2005-09-01T00:00:00Z
Publisher	Universidade Federal de Minas Gerais
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats

M.A.V. Barros / E.B. Andrade / R.G.N. Barros / I.K.M. Costa / I.C.L. Costa / G.F.A. Vitorino / J.J.C. Andrade / K.M. Paulino-Silva / V.O. Nogueira / J.L. de Brito Alves / J.H. Costa-Silva

Brazilian Journal of Medical and Biological Research, Vol 51, Iss

2018 Volume 6

Abstract: The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, ...

Abstract	The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.
Keywords	Development ; Epigenetics ; Fertility ; Gestation ; Intrauterine growth ; Nutrition ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	630
Language	English
Publishing date	2018-05-01T00:00:00Z
Publisher	Associação Brasileira de Divulgação Científica
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Serotonin modulation in neonatal age does not impair cardiovascular physiology in adult female rats: Hemodynamics and oxidative stress analysis

Braz, Glauber Ruda F / Aline Isabel da Silva / Anderson Apolonio Pedroza / Claudia J. Lagranha / Cristiane de Moura Freitas / João Henrique Costa-Silva / José Luiz de Brito Alves / Monique Assis de Vasconcelos Barros / Viviane O. Nogueira

Life sciences. 2016 Jan. 15, v. 145

2016

Abstract: The present study investigates the effects of neonatal serotonin modulation in female rats on cardiac parameters related to hemodynamics and oxidative metabolism in the mature animal.Female Wistar rat pups were administered daily subcutaneous injections ... ...

Abstract	The present study investigates the effects of neonatal serotonin modulation in female rats on cardiac parameters related to hemodynamics and oxidative metabolism in the mature animal.Female Wistar rat pups were administered daily subcutaneous injections of fluoxetine (Fx-treated group) or vehicle solution (Ct-group) from the 1st to 21st day of life. At 60days of age, animals from both groups were either used for cardiovascular evaluation or sacrificed for tissue collection for biochemical assays.We found that body weight in the Fx-treated group was less than that in the control. When analyzing hemodynamic parameters (i.e., arterial blood pressure, heart rate—HR, sympathetic and vagal tonus, or intrinsic HR), we did not observe significant difference in the Fx-treated group. Evaluating oxidative stress in brainstem and heart by measuring carbonyl content and malondialdehyde—MDA formation, we observe a decrease in carbonyl content only in the Fx-treated group (60.3%, in brainstem; 58.2%, in heart), without difference in the MDA levels. This observation is consonant with an increase in superoxide dismutase—SOD and catalase—CAT activity in brainstem and heart in the Fx-treated group (SOD: 82.7% and CAT: 23.7 in brainstem; SOD: 60.6%, and CAT: 40.7 in heart), with no changes in glutathione S-transferase activity and reduced glutathione levels. With regard to oxidative metabolism markers, citrate synthase activity was higher in brainstem in the Fx-treated group (20%).Our data suggest that serotonin modulation by Fx-treatment at an early age does not induce hemodynamic alteration, although it modulates oxidative metabolism in cardiac-related tissues.
Keywords	adults ; aerobiosis ; blood pressure ; body weight ; brain stem ; citrate (si)-synthase ; enzyme activity ; females ; glutathione ; glutathione transferase ; heart ; oxidative stress ; pups ; rats ; serotonin ; superoxide dismutase ; tissues
Language	English
Dates of publication	2016-0115
Size	p. 42-50.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	3378-9
ISSN	1879-0631 ; 0024-3205
ISSN (online)	1879-0631
ISSN	0024-3205
DOI	10.1016/j.lfs.2015.12.024
Database	NAL-Catalogue (AGRICOLA)

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