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  1. Article ; Online: Anthrax toxins-producing Bacillus spp. isolated from handwashing stations during COVID-19 pandemic in Lagos, Nigeria.

    Bamidele, Tajudeen A / Odumosu, Bamidele T / Adenola, Princess T / Ameh, James / Kareem, Olaide K / Osoba, Babatunde / Ezechi, Oliver C / Salako, Babatunde L

    Journal of infection in developing countries

    2023  Volume 17, Issue 8, Page(s) 1076–1080

    Abstract: Introduction: The virulence binding factor, protective antigen (pag) and poly-D-γ-glutamate capsular (cap) genes, peculiar to Bacillus anthracis are located in the pXO1 and pXO2 plasmids which are transferable horizontally to related species called " ... ...

    Abstract Introduction: The virulence binding factor, protective antigen (pag) and poly-D-γ-glutamate capsular (cap) genes, peculiar to Bacillus anthracis are located in the pXO1 and pXO2 plasmids which are transferable horizontally to related species called "cereus group". The cereus group are usually isolated from the environmental/food samples and have been implicated in debilitating human and animal anthrax-like diseases. This study was designed to investigate the presence of the anthrax virulence genes in different Bacillus spp. isolated from handwashing facilities during COVID-19 pandemic in Lagos, Nigeria.
    Methodology: The Bacillus anthracis (OK316847), B. thuringiensis (OK316855), B. amyloliquefaciens (OK316857), B. cereus (OK316858) and B. thuringiensis (OK316859) previously isolated from rinsates and bowl water in two local government areas (LGAs) of Lagos state were further investigated by the polymerase chain reaction (PCR) amplification of the pag and cap genes using specific primers.
    Results: Bacillus anthracis and B. cereus co-harboured the two 578 bp cap and 364 bp pag genes while B. thuringiensis only harboured the cap gene. Similarly, the non-cereus B. amyloliquefaciens was found to habour the pag gene.
    Conclusions: The two anthrax toxin genes were amplified in the Bacillus spp isolated from rinsates and bowl water used in hand washing in the two study LGAs. Given that these virulence genes have a global consequence and are a potential threat to life, this study calls for an extensive surveillance, and reassessment of gene regulators and plasmid distribution among these strains in our environment.
    MeSH term(s) Animals ; Humans ; Bacillus ; Hand Disinfection ; Anthrax/epidemiology ; Anthrax/prevention & control ; Nigeria/epidemiology ; Pandemics/prevention & control ; COVID-19/epidemiology ; COVID-19/prevention & control
    Chemical Substances anthrax toxin
    Language English
    Publishing date 2023-08-31
    Publishing country Italy
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2394024-4
    ISSN 1972-2680 ; 2036-6590
    ISSN (online) 1972-2680
    ISSN 2036-6590
    DOI 10.3855/jidc.18228
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: First Detection of OXA-10 Extended-Spectrum Beta-Lactamases and the Occurrence of mexR and nfxB in Clinical Isolates of Pseudomonas aeruginosa from Nigeria.

    Odumosu, Bamidele T / Adeniyi, Bola A / Chandra, Ram

    Chemotherapy

    2016  Volume 61, Issue 2, Page(s) 87–92

    Abstract: Background: The characterization of β-lactamase production in Pseudomonasaeruginosa is rarely reported in Nigeria. The objective of this study was to investigate the occurrence and characterize the different β-lactamases as well as mechanisms of ... ...

    Abstract Background: The characterization of β-lactamase production in Pseudomonasaeruginosa is rarely reported in Nigeria. The objective of this study was to investigate the occurrence and characterize the different β-lactamases as well as mechanisms of fluoroquinolones resistance among P. aeruginosa isolated from various clinical sources from Nigeria.
    Materials and method: Isolates were investigated using PCR, RFLP and sequencing for the detection of various β-lactamases and efflux pump regulator genes.
    Result: The prevalence of OXA-10, AmpC, CTX-M and SHV in P. aeruginosa was 80, 70, 5 and 5%, respectively. The coexistence of blaOXA-10 with blaAmpC, blaSHV and blaCTX-M was reported in 40, 5 and 5% of isolates, respectively. The efflux pump regulator genes mexR and nfxB were both amplified in 45% of the OXA-10-positive isolates.
    Conclusion: This is the first report of the characterization of OXA-10 extended-spectrum β-lactamases and occurrence of mexR and nfxB efflux regulator genes in clinical isolates of P. aeruginosa in Nigeria.
    MeSH term(s) Bacterial Proteins/genetics ; DNA-Binding Proteins/genetics ; Drug Resistance, Bacterial/drug effects ; Fluoroquinolones/pharmacology ; Gene Expression Regulation, Bacterial/drug effects ; Humans ; Nigeria ; Polymorphism, Restriction Fragment Length ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/isolation & purification ; Repressor Proteins/genetics ; Transcription Factors/genetics ; beta-Lactamases/genetics
    Chemical Substances Bacterial Proteins ; DNA-Binding Proteins ; Fluoroquinolones ; MexR protein, Pseudomonas aeruginosa ; NfxB protein, Pseudomonas aeruginosa ; Repressor Proteins ; Transcription Factors ; beta-lactamase PSE-2 (EC 3.5.2.-) ; AmpC beta-lactamases (EC 3.5.2.6) ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2016
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 6708-8
    ISSN 1421-9794 ; 0009-3157
    ISSN (online) 1421-9794
    ISSN 0009-3157
    DOI 10.1159/000441712
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  3. Article ; Online: Analysis of integrons and associated gene cassettes in clinical isolates of multidrug resistant Pseudomonas aeruginosa from Southwest Nigeria.

    Odumosu, Bamidele T / Adeniyi, Bolanle A / Chandra, Ram

    Annals of clinical microbiology and antimicrobials

    2013  Volume 12, Page(s) 29

    Abstract: Background: Multidrug resistant Pseudomonas aeruginosa harbours integrons and other mobile genetic elements such as plasmids and transposons, which easily disseminate antibiotic resistance genes among clinical strains of P. aeruginosa.: Methodology: ... ...

    Abstract Background: Multidrug resistant Pseudomonas aeruginosa harbours integrons and other mobile genetic elements such as plasmids and transposons, which easily disseminate antibiotic resistance genes among clinical strains of P. aeruginosa.
    Methodology: Plasmid extraction of 54 clinical isolates of P. aeruginosa was carried out by alkaline lysis method; and plasmid size estimation was done by using E. coli V517 standard plasmid marker. Fifty-four clinical strains of P. aeruginosa were isolated from 5 hospitals in 3 Southwestern states of Nigeria between March and September 2010. Plasmid extraction of isolates was carried out by alkaline lysis method; and plasmid size estimation was done by using E. coli V517 standard plasmid marker. PCR amplification for the 3 classes of resistance integrons, and gene cassette characterization were carried out using specific primers and by sequencing of PCR products. Conjugal mating of the integron positive P. aeruginosa strains with E. coli DH5α was performed to demonstrate transferability of integrons and gene cassettes.
    Result: Agarose gel electrophoresis of plasmid DNA revealed that all the 54 P. aeruginosa harboured 1-4 plasmids with sizes ranging from 2.2 - >58 kb. Class 1 integron was identified in 31 (57%) strains; but none of them carried class 2 and class 3 integrons. High prevalence of aadA gene conferring resistance to streptomycin/spectinomycin was detected in the strains positive for class 1 integron. Sequencing of the 1.6 kb and 1.2 kb amplified band of gene cassettes revealed the presence of aadA6-orfD and aadA13 respectively.
    Conclusion: This study demonstrates the presence of plasmids and integrons harbouring resistance gene cassettes, which may collectively constitute an efficient system for dissemination of resistance genes in P. aeruginosa. Disturbingly, the rapid and unabated spread of class 1 integron-associated multidrug resistant P. aeruginosa in Southwest Nigeria may greatly hamper successful treatment of infections caused by such strains. This necessitates the establishment of functional antimicrobial resistance surveillance programmes in Nigeria.
    MeSH term(s) Conjugation, Genetic ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Drug Resistance, Multiple, Bacterial ; Hospitals ; Humans ; Integrons ; Molecular Sequence Data ; Nigeria ; Plasmids ; Polymerase Chain Reaction ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/isolation & purification ; Sequence Analysis, DNA
    Chemical Substances DNA, Bacterial
    Language English
    Publishing date 2013-10-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2097873-X
    ISSN 1476-0711 ; 1476-0711
    ISSN (online) 1476-0711
    ISSN 1476-0711
    DOI 10.1186/1476-0711-12-29
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Antimicrobial activities of streptomyces ceolicolor strain aob kf977550 isolated from a tropical estuary

    Odumosu, Bamidele T / Chiamaka J. Okeke / Frederick C. Michel / James O. Ogah / Olanike M. Buraimoh

    Journal of Taibah University for Science. 2017,

    2017  

    Abstract: The aim of this study is to screen for antibiotically important Streptomyces from a tropical estuary. Five bacterial strains identified by 16S rDNA gene sequencing as Streptomyces albogriseolus, S. aureus, S. coelicolor, S. albus, and S. pseudogriseolus ... ...

    Abstract The aim of this study is to screen for antibiotically important Streptomyces from a tropical estuary. Five bacterial strains identified by 16S rDNA gene sequencing as Streptomyces albogriseolus, S. aureus, S. coelicolor, S. albus, and S. pseudogriseolus were isolated from the Lagos Lagoon. Ethyl acetate extracts of the test Streptomyces spp fermented broths were evaluated against laboratory strains of MRSA Staphylococcus aureus 144m, Bacillus coagulans UL001, Escherichia coli and standard strains Klebsiella pneumonia ATCC 8308, Gardnerella vaginalis ATCC 27853 and Salmonella typhi ATCC 13311 using the well diffusion method. The presence of secondary metabolite was determined and analyzed using gas chromatography-mass spectrometer (GC-MS). Broad spectrum of activity was observed for only S. coelicolor on all the tested bacteria except S. typhi, GC-MS analysis revealed the presence of 16 secondary metabolites with antibiotically relevant properties. The result of this study suggest Lagos lagoon is a potential source and reservoir of novel antibiotics.
    Keywords antibiotics ; antimicrobial properties ; Bacillus coagulans ; bacteria ; culture media ; Escherichia coli ; estuaries ; ethyl acetate ; Gardnerella vaginalis ; gas chromatography-mass spectrometry ; Klebsiella pneumoniae ; methicillin-resistant Staphylococcus aureus ; Salmonella Typhi ; secondary metabolites ; sequence analysis ; spectrometers ; Streptomyces albogriseolus
    Language English
    Size p. .
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 2834710-9
    ISSN 1658-3655
    ISSN 1658-3655
    DOI 10.1016/j.jtusci.2017.01.006
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: First Detection of OXA-10 Extended-Spectrum Beta-Lactamases and the Occurrence of ; and ; in Clinical Isolates of ; from Nigeria

    Odumosu, Bamidele T. / Adeniyi, Bola A. / Chandra, Ram

    Chemotherapy

    2015  Volume 61, Issue 2, Page(s) 87–92

    Abstract: Background: The characterization of β-lactamase production in Pseudomonasaeruginosa is rarely reported in Nigeria. The objective of this study was to investigate the occurrence and characterize the different β-lactamases as well as mechanisms of ... ...

    Institution Department of Microbiology, University of Lagos, Lagos, and Department of Pharmaceutical Microbiology, University of Ibadan, Ibadan, Nigeria Environmental Microbiology Section, CSIR-Indian Institute of Toxicology Research, Lucknow, India
    Abstract Background: The characterization of β-lactamase production in Pseudomonasaeruginosa is rarely reported in Nigeria. The objective of this study was to investigate the occurrence and characterize the different β-lactamases as well as mechanisms of fluoroquinolones resistance among P. aeruginosa isolated from various clinical sources from Nigeria. Materials and Method: Isolates were investigated using PCR, RFLP and sequencing for the detection of various β-lactamases and efflux pump regulator genes. Result: The prevalence of OXA-10, AmpC, CTX-M and SHV in P. aeruginosa was 80, 70, 5 and 5%, respectively. The coexistence of blaOXA-10 with blaAmpCblaSHV and blaCTX-M was reported in 40, 5 and 5% of isolates, respectively. The efflux pump regulator genes mexR and nfxB were both amplified in 45% of the OXA-10-positive isolates. Conclusion: This is the first report of the characterization of OXA-10 extended-spectrum β-lactamases and occurrence of mexR and nfxB efflux regulator genes in clinical isolates of P. aeruginosa in Nigeria.
    Keywords β-Lactamases ; Resistance ; Efflux pumps
    Language English
    Publishing date 2015-11-26
    Publisher S. Karger AG
    Publishing place Basel, Switzerland
    Document type Article
    Note Antimicrobial Section / Original Paper
    ZDB-ID 6708-8
    ISSN 1421-9794 ; 0009-3157
    ISSN (online) 1421-9794
    ISSN 0009-3157
    DOI 10.1159/000441712
    Database Karger publisher's database

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    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.174: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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