Article ; Online: Cluster J mycobacteriophages: intron splicing in capsid and tail genes.
PloS one
2013 Volume 8, Issue 7, Page(s) e69273
Abstract: Bacteriophages isolated on Mycobacterium smegmatis mc(2)155 represent many distinct genomes sharing little or no DNA sequence similarity. The genomes are architecturally mosaic and are replete with genes of unknown function. A new group of genomes ... ...
| Abstract | Bacteriophages isolated on Mycobacterium smegmatis mc(2)155 represent many distinct genomes sharing little or no DNA sequence similarity. The genomes are architecturally mosaic and are replete with genes of unknown function. A new group of genomes sharing substantial nucleotide sequences constitute Cluster J. The six mycobacteriophages forming Cluster J are morphologically members of the Siphoviridae, but have unusually long genomes ranging from 106.3 to 117 kbp. Reconstruction of the capsid by cryo-electron microscopy of mycobacteriophage BAKA reveals an icosahedral structure with a triangulation number of 13. All six phages are temperate and homoimmune, and prophage establishment involves integration into a tRNA-Leu gene not previously identified as a mycobacterial attB site for phage integration. The Cluster J genomes provide two examples of intron splicing within the virion structural genes, one in a major capsid subunit gene, and one in a tail gene. These genomes also contain numerous free-standing HNH homing endonuclease, and comparative analysis reveals how these could contribute to genome mosaicism. The unusual Cluster J genomes provide new insights into phage genome architecture, gene function, capsid structure, gene mobility, intron splicing, and evolution. |
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| MeSH term(s) | Amino Acid Sequence ; Bacteriolysis/genetics ; Base Composition ; Base Sequence ; Capsid Proteins/chemistry ; Capsid Proteins/genetics ; Cluster Analysis ; DNA Transposable Elements ; Gene Order ; Genome Size ; Genome, Viral ; Introns ; Molecular Sequence Data ; Mycobacteriophages/classification ; Mycobacteriophages/genetics ; Mycobacteriophages/ultrastructure ; Open Reading Frames ; Phylogeny ; RNA Splicing ; Viral Tail Proteins/chemistry ; Viral Tail Proteins/genetics ; Virion/ultrastructure ; Virus Integration/genetics |
| Chemical Substances | Capsid Proteins ; DNA Transposable Elements ; Viral Tail Proteins |
| Language | English |
| Publishing date | 2013-07-09 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0069273 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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