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  1. Article: A mask-based diagnostic platform for point-of-care screening of Covid-19

    Daniels, John / Wadekar, Shekhar / DeCubellis, Ken / Jackson, George W. / Chiu, Alexander S. / Pagneux, Quentin / Saada, Hiba / Engelmann, Ilka / Ogiez, Judith / Loze-Warot, Delphine / Boukherroub, Rabah / Szunerits, Sabine

    Biosensors & bioelectronics. 2021 Nov. 15, v. 192

    2021  

    Abstract: Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self- ... ...

    Abstract Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200–500 μL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL⁻¹ in cultured SARS-CoV-2 suspensions. Using a “turn off” assay via ferrocenemethanol redox mediator, results about the infectivity state of the patient are obtained in 10 min.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; biosensors ; condensates ; diagnostic techniques ; electrochemistry ; face masks ; oligonucleotides ; pathogenicity ; patients ; point-of-care systems ; reverse transcriptase polymerase chain reaction ; vapors
    Language English
    Dates of publication 2021-1115
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2021.113486
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Preanalytical Issues and Cycle Threshold Values in SARS-CoV-2 Real-Time RT-PCR Testing: Should Test Results Include These?

    Engelmann, Ilka / Alidjinou, Enagnon Kazali / Ogiez, Judith / Pagneux, Quentin / Miloudi, Sana / Benhalima, Ilyes / Ouafi, Mahdi / Sane, Famara / Hober, Didier / Roussel, Alain / Cambillau, Christian / Devos, David / Boukherroub, Rabah / Szunerits, Sabine

    ACS omega

    2021  Volume 6, Issue 10, Page(s) 6528–6536

    Abstract: Since the emergence of SARS-CoV-2 pandemic, clinical laboratories worldwide are overwhelmed with SARS-CoV-2 testing using the current gold standard: real-time reverse-transcription polymerase chain reaction (RT-PCR) assays. The large numbers of suspected ...

    Abstract Since the emergence of SARS-CoV-2 pandemic, clinical laboratories worldwide are overwhelmed with SARS-CoV-2 testing using the current gold standard: real-time reverse-transcription polymerase chain reaction (RT-PCR) assays. The large numbers of suspected cases led to shortages in numerous reagents such as specimen transport and RNA extraction buffers. We try to provide some answers on how strongly preanalytical issues affect RT-PCR results by reviewing the utility of different transport buffer media and virus inactivation procedures and comparing the literature data with our own recent findings. We show that various viral inactivation procedures and transport buffers are available and are less of a bottleneck for PCR-based methods. However, efficient alternative lysis buffers remain more difficult to find, and several fast RT-PCR assays are not compatible with guanidine-containing media, making this aspect more of a challenge in the current crisis. Furthermore, the availability of different SARS-CoV-2-specific RT-PCR kits with different sensitivities makes the definition of a general cutoff level for the cycle threshold (Ct) value challenging. Only a few studies have considered how Ct values relate to viral infectivity and how preanalytical issues might affect viral infectivity and RNA detection. We review the current data on the correlation between Ct values and viral infectivity. The presence of the SARS-CoV-2 viral genome in its own is not sufficient proof of infectivity and caution is needed in evaluation of the infectivity of samples. The correlation between Ct values and viral infectivity revealed an RT-PCR cutoff value of 34 cycles for SARS-CoV-2 infectivity using a laboratory-developed RT-PCR assay targeting the RdRp gene. While ideally each clinical laboratory should perform its own correlation, we believe this perspective article could be a reference point for others, in particular medical doctors and researchers interested in COVID-19 diagnostics, and a first step toward harmonization.
    Language English
    Publishing date 2021-03-06
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.1c00166
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: SARS-CoV-2 detection using a nanobody-functionalized voltammetric device.

    Pagneux, Quentin / Roussel, Alain / Saada, Hiba / Cambillau, Christian / Amigues, Béatrice / Delauzun, Vincent / Engelmann, Ilka / Alidjinou, Enagnon Kazali / Ogiez, Judith / Rolland, Anne Sophie / Faure, Emmanuel / Poissy, Julien / Duhamel, Alain / Boukherroub, Rabah / Devos, David / Szunerits, Sabine

    Communications medicine

    2022  Volume 2, Page(s) 56

    Abstract: Background: An ongoing need during the COVID-19 pandemic has been the requirement for accurate and efficient point-of-care testing platforms to distinguish infected from non-infected people, and to differentiate SARS-CoV-2 infections from other viruses. ...

    Abstract Background: An ongoing need during the COVID-19 pandemic has been the requirement for accurate and efficient point-of-care testing platforms to distinguish infected from non-infected people, and to differentiate SARS-CoV-2 infections from other viruses. Electrochemical platforms can detect the virus via its envelope spike protein by recording changes in voltammetric signals between samples. However, this remains challenging due to the limited sensitivity of these sensing platforms.
    Methods: Here, we report on a nanobody-functionalized electrochemical platform for the rapid detection of whole SARS-CoV-2 viral particles in complex media such as saliva and nasopharyngeal swab samples. The sensor relies on the functionalization of gold electrode surface with highly-oriented Llama nanobodies specific to the spike protein receptor binding domain (RBD). The device provides results in 10 min of exposure to 200 µL of unprocessed samples with high specificity to SARS-CoV-2 viral particles in human saliva and nasopharyngeal swab samples.
    Results: The developed sensor could discriminate between different human coronavirus strains and other respiratory viruses, with 90% positive and 90% negative percentage agreement on 80 clinical samples, as compared to RT-qPCR.
    Conclusions: We believe this diagnostic concept, also validated for RBD mutants and successfully tested on Delta variant samples, to be a powerful tool to detect patients' infection status, easily extendable to other viruses and capable of overcoming sensing-related mutation effects.
    Language English
    Publishing date 2022-05-23
    Publishing country England
    Document type Journal Article
    ISSN 2730-664X
    ISSN (online) 2730-664X
    DOI 10.1038/s43856-022-00113-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A mask-based diagnostic platform for point-of-care screening of Covid-19.

    Daniels, John / Wadekar, Shekhar / DeCubellis, Ken / Jackson, George W / Chiu, Alexander S / Pagneux, Quentin / Saada, Hiba / Engelmann, Ilka / Ogiez, Judith / Loze-Warot, Delphine / Boukherroub, Rabah / Szunerits, Sabine

    Biosensors & bioelectronics

    2021  Volume 192, Page(s) 113486

    Abstract: Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self- ... ...

    Abstract Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200-500 μL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL
    Language English
    Publishing date 2021-07-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2021.113486
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Relapsing Pityriasis Rosea With HHV-7 Reactivation in an 11-Year-Old Girl.

    Engelmann, Ilka / Ogiez, Judith / Ogiez, Lucie / Alidjinou, Enagnon Kazali / Lazrek, Mouna / Dewilde, Anny / Hober, Didier

    Pediatrics

    2018  Volume 141, Issue 5

    Abstract: Pityriasis rosea (PR) usually presents as acute exanthema with oval erythematous-squamous lesions localized on the trunk, arms, and legs with spontaneous remission. We present an unusual case of PR with frequent relapses during a period of 7 years. An 11- ...

    Abstract Pityriasis rosea (PR) usually presents as acute exanthema with oval erythematous-squamous lesions localized on the trunk, arms, and legs with spontaneous remission. We present an unusual case of PR with frequent relapses during a period of 7 years. An 11-year-old white female patient presented with many pruritic erythematous oval lesions on her trunk. A second episode followed 2 years later with several pruritic erythematous lesions on her lower limbs. During the following 5 years, the patient had several relapses per year, with 1 to 3 lesions on changing localizations. PR was diagnosed on the basis of the clinical presentation and detection of human herpesvirus 7 DNA. Spontaneous remission occurred without treatment in each episode. Relapsing PR is a rare form of PR characterized by a lower number of lesions and smaller sized lesions compared with the classic form of PR. Pediatricians should consider the diagnosis of relapsing PR even if only a single or few erythematous lesions are present.
    MeSH term(s) Child ; DNA, Viral/analysis ; Female ; Herpesvirus 7, Human/genetics ; Herpesvirus 7, Human/physiology ; Humans ; Pityriasis Rosea/pathology ; Pityriasis Rosea/psychology ; Pityriasis Rosea/virology ; Recurrence ; Remission, Spontaneous ; Stress, Psychological ; Virus Activation
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2018-04-19
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 207677-9
    ISSN 1098-4275 ; 0031-4005
    ISSN (online) 1098-4275
    ISSN 0031-4005
    DOI 10.1542/peds.2017-3179
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    Zs.MO 357: Show issues

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  6. Article ; Online: Comparison of two commercial quantitative PCR assays for EBV DNA detection and their correlation with the first WHO International Standard for EBV.

    Engelmann, Ilka / Alidjinou, Enagnon Kazali / Lazrek, Mouna / Pouillaude, Jean-Marie / Ogiez, Judith / Rose, François / Duhamel, Alain / Dewilde, Anny / Hober, Didier

    Journal of medical microbiology

    2018  Volume 67, Issue 4, Page(s) 529–536

    Abstract: Purpose: There are few data on the performance of automated Epstein-Barr virus (EBV) PCR assays. This study compared EBV quantification for the kPCR PLX EBV DNA (kPCR; Siemens, France) and the EBV R-gene (R-gene; Argene, Biomerieux, France) assays and ... ...

    Abstract Purpose: There are few data on the performance of automated Epstein-Barr virus (EBV) PCR assays. This study compared EBV quantification for the kPCR PLX EBV DNA (kPCR; Siemens, France) and the EBV R-gene (R-gene; Argene, Biomerieux, France) assays and their correlation with the World Health Organization (WHO) standard.
    Methodology: WHO International Standard for EBV (WHO standard) dilution panels in different matrices were submitted to nucleic acid extraction with Versant kPCR Molecular Systems SP followed by the kPCR assay, or to nucleic acid extraction with the MagNA Pure LC System or NucliSENS easyMag followed by the R-gene assay. Seventy-four clinical specimens were tested in both assays. Bland-Altman analysis and linear regression analysis were performed.
    Results: The correlation between the WHO standard diluted in different matrices and the R-gene and kPCR assays was good (R
    Conclusions: The quantitative results of both assays showed reasonable correlation with each other and a good correlation with the WHO standard.
    MeSH term(s) DNA, Viral/genetics ; Epstein-Barr Virus Infections/diagnosis ; Epstein-Barr Virus Infections/virology ; Herpesvirus 4, Human/classification ; Herpesvirus 4, Human/genetics ; Herpesvirus 4, Human/isolation & purification ; Humans ; Real-Time Polymerase Chain Reaction/economics ; Real-Time Polymerase Chain Reaction/methods ; Real-Time Polymerase Chain Reaction/standards ; World Health Organization
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2018-02-26
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 218356-0
    ISSN 1473-5644 ; 0022-2615
    ISSN (online) 1473-5644
    ISSN 0022-2615
    DOI 10.1099/jmm.0.000702
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 634: Show issues Location:
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  7. Article ; Online: Comparison of two commercial quantitative PCR assays and correlation with the first WHO International Standard for human CMV.

    Engelmann, Ilka / Alidjinou, Enagnon Kazali / Lazrek, Mouna / Ogiez, Judith / Pouillaude, Jean-Marie / Chazard, Emmanuel / Dewilde, Anny / Hober, Didier

    Diagnostic microbiology and infectious disease

    2018  Volume 91, Issue 1, Page(s) 27–33

    Abstract: Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard). Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP or MagNA Pure LC System followed ... ...

    Abstract Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard). Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP or MagNA Pure LC System followed by the kPCR PLX™ CMV DNA (kPCR) or the CMV R-gene™ assay (R-gene), respectively; 139 clinical specimens were tested. Both assays correlated well with the standard (R
    MeSH term(s) Cytomegalovirus/genetics ; Cytomegalovirus/isolation & purification ; Cytomegalovirus Infections/virology ; DNA, Viral/blood ; DNA, Viral/urine ; Humans ; Linear Models ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Viral Load ; World Health Organization
    Chemical Substances DNA, Viral ; Reagent Kits, Diagnostic
    Language English
    Publishing date 2018-01-07
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2017.12.021
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