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  1. Article: The evolutionary diversification of the

    Ojiakor, Adaobi / Gibbs, Rachel N / Chen, Zhe / Gao, Xiang / Fowler, Casey C

    Frontiers in microbiology

    2022  Volume 13, Page(s) 1016438

    Abstract: Salmonella ... ...

    Abstract Salmonella enterica
    Language English
    Publishing date 2022-11-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.1016438
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Mechanistic insight into bacterial entrapment by septin cage reconstitution.

    Lobato-Márquez, Damián / Xu, Jingwei / Güler, Gizem Özbaykal / Ojiakor, Adaobi / Pilhofer, Martin / Mostowy, Serge

    Nature communications

    2021  Volume 12, Issue 1, Page(s) 4511

    Abstract: Septins are cytoskeletal proteins that assemble into hetero-oligomeric complexes and sense micron-scale membrane curvature. During infection with Shigella flexneri, an invasive enteropathogen, septins restrict actin tail formation by entrapping bacteria ... ...

    Abstract Septins are cytoskeletal proteins that assemble into hetero-oligomeric complexes and sense micron-scale membrane curvature. During infection with Shigella flexneri, an invasive enteropathogen, septins restrict actin tail formation by entrapping bacteria in cage-like structures. Here, we reconstitute septin cages in vitro using purified recombinant septin complexes (SEPT2-SEPT6-SEPT7), and study how these recognize bacterial cells and assemble on their surface. We show that septin complexes recognize the pole of growing Shigella cells. An amphipathic helix domain in human SEPT6 enables septins to sense positively curved membranes and entrap bacterial cells. Shigella strains lacking lipopolysaccharide components are more efficiently entrapped in septin cages. Finally, cryo-electron tomography of in vitro cages reveals how septins assemble as filaments on the bacterial cell surface.
    MeSH term(s) Actins/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cell Membrane/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Dysentery, Bacillary/metabolism ; Dysentery, Bacillary/microbiology ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; HeLa Cells ; Humans ; Lipopolysaccharides/metabolism ; Microscopy, Fluorescence ; Mutation ; Protein Binding ; Recombinant Proteins/metabolism ; Septins/genetics ; Septins/metabolism ; Shigella flexneri/genetics ; Shigella flexneri/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism
    Chemical Substances Actins ; Bacterial Proteins ; DNA-Binding Proteins ; Lipopolysaccharides ; Recombinant Proteins ; Transcription Factors ; virG protein, Shigella flexneri ; Green Fluorescent Proteins (147336-22-9) ; SEPTIN6 protein, human (EC 3.6.1.-) ; Septins (EC 3.6.1.-)
    Language English
    Publishing date 2021-07-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-021-24721-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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