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Article ; Online: Erratum: MIREILLE BELLE MBOU OKASSA, DOLLON MBAMA NTABI amp; ARSENE LENGA (2020) Morphological and molecular identification of specimens in the genus Euseius (Acari: Phytoseiidae) from the Republic of Congo. Zootaxa, 4768: 479-498.

Okassa, Mireille Belle Mbou / Ntabi, Dollon Mbama / Lenga, Arsene

Zootaxa

2021 Volume 4920, Issue 4, Page(s) zootaxa.4920.4.10

Language	English
Publishing date	2021-02-02
Publishing country	New Zealand
Document type	Journal Article ; Published Erratum
ISSN	1175-5334
ISSN (online)	1175-5334
DOI	10.11646/zootaxa.4920.4.10
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Article: Staging's determinants in early intervention youth: a retrospective study.

Hamdan, Mirvat / Lecardeur, Laurent / Habert, Marine / Okassa, Mireille / Lacroix, Aurélie / Calvet, Benjamin

L'Encephale

2023

Abstract: Objectives: Since 2019 our early intervention unit has assessed help-seekers, mainly referred by psychiatric departments, and we have conducted a descriptive retrospective study. Our objective was to identify clinical determinants associated to staging ... ...

Abstract	Objectives: Since 2019 our early intervention unit has assessed help-seekers, mainly referred by psychiatric departments, and we have conducted a descriptive retrospective study. Our objective was to identify clinical determinants associated to staging at assessment for our three groups: "no psychosis", "ultra-high risk" and "first episode psychosis". Methods: One hundred and thirteen participants (mean age 20.05±3.28) were enrolled, mainly referred by adult psychiatry (81.4%). We tested the association of each group with the following determinants: age, gender, family history of psychosis, referral (adolescent or adult psychiatry), cognitive, depressive complaint, cannabis active consumption, and current activity (scholar or employment). Results: Multivariate analyses showed significant association with depressive symptoms (P=0.019) but an absence of family history of psychosis (P=0.002) or current activity (P=0.09) for "no psychosis" group. "Ultra-high risk" was significantly correlated with a family history of psychosis (P=0.001) and adolescent psychiatry referral (P=0.044) but an absence of depressive complaint (P=0.04). As for "first episode psychosis", we found significant cognitive complaint (P=0.026), family history (P=0.024) and current activity (0.026). Conclusions: As all our participants were seen in tertiary care, adolescent psychiatrists were more efficient in detecting a high-risk state. "No psychosis" help-seekers presented in fact mood issues, which have been confused with attenuated psychotic symptoms by their addressers, who have probably been misled by their absence of activity integration. High-risk and characterized psychotic episodes were logically correlated with family history. Surprisingly, "first episode psychosis" youth were currently integrated in scholarly or professional life despite an active cognitive complaint. Robust studies, especially prospective cohorts, are needed to test these associations.
Language	English
Publishing date	2023-11-18
Publishing country	France
Document type	Journal Article
ZDB-ID	214431-1
ISSN	0013-7006
ISSN	0013-7006
DOI	10.1016/j.encep.2023.08.015
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Article ; Online: Morphological and molecular identification of specimens in the genus Euseius (Acari: Phytoseiidae) from the Republic of Congo.

Okassa, Mireille Belle Mbou / Ntabi, Dollon Mbama / Lenga, Arsene

Zootaxa

2020 Volume 4768, Issue 4, Page(s) zootaxa.4768.4.2

Abstract: The purpose of this study was to identify the taxonomic status of several individuals belonging to the genus Euseius, collected from three host plants (cassava, okra, and chilli) in the Republic of the Congo, using morphometric and molecular analysis. ... ...

Abstract	The purpose of this study was to identify the taxonomic status of several individuals belonging to the genus Euseius, collected from three host plants (cassava, okra, and chilli) in the Republic of the Congo, using morphometric and molecular analysis. For this, hierarchical ascending classification on principal component analysis was performed using morphological measurements from 21 and 22 individuals of E. fustis and Euseius sp., respectively, with the original description of Euseius neodossei considered. For molecular analysis, two DNA fragments were amplified and sequenced: the mitochondrial 12S rRNA and the nuclear ribosomal region ITSS. This integrative approach found that (1) morphological differences existed between the Euseius sp. and both E. neodossei and E. fustis, and (2) both mitochondrial DNA fragments showed a clear delineation between Euseius sp. and E. fustis. The results obtained highlight the value of using integrative taxonomy to improve the identification of species in under-sampled areas and the need to increase the number of DNA sequences deposited in GenBank database from this region. This is the first time that DNA sequences of mites from the Congo (Brazzaville) have been deposited in this database.
MeSH term(s)	Animals ; Congo ; DNA, Mitochondrial ; Mites ; Mitochondria
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2020-05-05
Publishing country	New Zealand
Document type	Journal Article
ISSN	1175-5334
ISSN (online)	1175-5334
DOI	10.11646/zootaxa.4768.4.2
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Article: Molecular characterization of pests (Chrysomelidae: Bruchinae) of beans (Phaseolus vulgaris) in the Republic of Congo

Dibangou, Valentin / Okassa, Mireille Belle Mbou / Mazikou, Grace Fidelma Missamou / Lenga, Arsène

African zoology. 2021 Jan. 02, v. 56, no. 1

2021

Abstract: The bean Phaseolus vulgaris is widely consumed by the Congolese population, because it is rich in protein and has a good nutritional quality. Beetles of the subfamily Bruchinae are pests of bean seeds in the field and storage in the Republic of Congo (RC) ...

Abstract	The bean Phaseolus vulgaris is widely consumed by the Congolese population, because it is rich in protein and has a good nutritional quality. Beetles of the subfamily Bruchinae are pests of bean seeds in the field and storage in the Republic of Congo (RC). Their attacks cause a reduction in seed weight, leading to a decrease in the nutritional and commercial value of the seeds. The purpose of our study was to identify the post-harvest diversity of bean bruchids in two bean producing regions in the Congo (Niari and Bouenza) using molecular taxonomy (12S rRNA marker). The bean weevil, Acanthoscelides obtectus (Say), and cowpea weevil, Callosobruchus maculatus (Fabricius), were identified. Acanthoscelides obtectus, recognised as the main pest of beans, was found in all ten localities sampled, whereas C. maculatus, recognised as the main pest of pigeon peas (Cajanus cajan) and chickpeas (Cicer arietinum), was found in two of the ten. Better knowledge of the diversity of bruchids present in the RC will make it possible to propose effective control methods with little impact on environmental and human health.
Keywords	Acanthoscelides obtectus ; Cajanus cajan ; Callosobruchus maculatus ; Chrysomelidae ; Cicer arietinum ; Phaseolus vulgaris ; human health ; molecular systematics ; nutritive value ; pests ; pigeons ; seed weight ; zoology ; Republic of the Congo
Language	English
Dates of publication	2021-0102
Size	p. 35-43.
Publishing place	Taylor & Francis
Document type	Article
Note	NAL-light
ZDB-ID	2070936-5
ISSN	2224-073X ; 1562-7020
ISSN (online)	2224-073X
ISSN	1562-7020
DOI	10.1080/15627020.2020.1848455
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Article ; Online: Distinct Predictors of Clinical Response after Repetitive Transcranial Magnetic Stimulation between Bipolar and Unipolar Disorders.

Lacroix, Aurélie / Paquet, Aude / Okassa, Mireille / Vinais, Théodore / Lannaud, Marilyne / Plansont, Brigitte / Buisson, Alexandre / Guignandon, Sandrine / Malauzat, Dominique / Girard, Murielle / Calvet, Benjamin

International journal of environmental research and public health

2023 Volume 20, Issue 7

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Humans ; Transcranial Magnetic Stimulation/methods ; Bipolar Disorder/therapy ; Anxiety Disorders ; Treatment Outcome ; Prefrontal Cortex
Language	English
Publishing date	2023-03-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20075276
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Article ; Online: Morphological and molecular diagnostics of Phytoseiulus persimilis and Phytoseiulus macropilis (Acari: Phytoseiidae).

Okassa, Mireille / Tixier, Marie-Stéphane / Kreiter, Serge

Experimental & applied acarology

2010 Volume 52, Issue 3, Page(s) 291–303

Abstract: This study focuses on the diagnostics of two natural enemy species, belonging to the genus Phytoseiulus in the family Phytoseiidae (sub-family Amblyseiinae): P. macropilis and P. persimilis. These two species are of primary importance in biological ... ...

Abstract	This study focuses on the diagnostics of two natural enemy species, belonging to the genus Phytoseiulus in the family Phytoseiidae (sub-family Amblyseiinae): P. macropilis and P. persimilis. These two species are of primary importance in biological control all over the world. However, they are morphologically very similar and specific diagnostics is difficult. This study utilizes mitochondrial molecular markers (12S rRNA and Cytb mtDNA) to differentiate these two species. Morphological analyses showed significant differences between P. persimilis and P. macropilis for 17 morphological characters of the 32 considered. However, despite these significant differences, the ranges of all characters overlap. Only the serration of the macroseta on the basitarsus (StIV) allows the differentiation between P. persimilis and P. macropilis. Despite these small morphological differences, molecular results, for both mitochondrial DNA fragments considered (rRNA and Cytb mtDNA), showed a clear delineation between the specimens of P. macropilis and P. persimilis. This study emphasizes (i) that only one morphological character (serration of the seta StIV) clearly separates these two species, and (ii) the usefulness of an automatical molecular and simple diagnostic tool for accurate differentiation of the two species and ensure the morphological diagnostics. Further studies are proposed, including more DNA sequences especially for P. macropilis.
MeSH term(s)	Animals ; Cytochromes b/chemistry ; DNA, Mitochondrial/chemistry ; Female ; Mites/anatomy & histology ; Mites/classification ; Mites/genetics ; Molecular Sequence Data ; Pest Control, Biological ; RNA, Ribosomal/chemistry ; Species Specificity
Chemical Substances	DNA, Mitochondrial ; RNA, Ribosomal ; RNA, ribosomal, 12S ; Cytochromes b (9035-37-4)
Language	English
Publishing date	2010-05-16
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	11320-7
ISSN	1572-9702 ; 0168-8162
ISSN (online)	1572-9702
ISSN	0168-8162
DOI	10.1007/s10493-010-9364-x
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Article: Morphological and molecular diagnostics of Phytoseiulus persimilis and Phytoseiulus macropilis (Acari: Phytoseiidae)

Okassa, Mireille / Tixier, Marie-Stéphane / Kreiter, Serge

Experimental and applied acarology. 2010 Nov., v. 52, no. 3

2010

Abstract	This study focuses on the diagnostics of two natural enemy species, belonging to the genus Phytoseiulus in the family Phytoseiidae (sub-family Amblyseiinae): P. macropilis and P. persimilis. These two species are of primary importance in biological control all over the world. However, they are morphologically very similar and specific diagnostics is difficult. This study utilizes mitochondrial molecular markers (12S rRNA and Cytb mtDNA) to differentiate these two species. Morphological analyses showed significant differences between P. persimilis and P. macropilis for 17 morphological characters of the 32 considered. However, despite these significant differences, the ranges of all characters overlap. Only the serration of the macroseta on the basitarsus (StIV) allows the differentiation between P. persimilis and P. macropilis. Despite these small morphological differences, molecular results, for both mitochondrial DNA fragments considered (rRNA and Cytb mtDNA), showed a clear delineation between the specimens of P. macropilis and P. persimilis. This study emphasizes (i) that only one morphological character (serration of the seta StIV) clearly separates these two species, and (ii) the usefulness of an automatical molecular and simple diagnostic tool for accurate differentiation of the two species and ensure the morphological diagnostics. Further studies are proposed, including more DNA sequences especially for P. macropilis.
Keywords	Phytoseiulus ; taxonomy
Language	English
Dates of publication	2010-11
Size	p. 291-303.
Publisher	Springer Netherlands
Publishing place	Dordrecht
Document type	Article
ZDB-ID	11320-7
ISSN	1572-9702 ; 0168-8162
ISSN (online)	1572-9702
ISSN	0168-8162
DOI	10.1007/s10493-010-9364-x
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Article: Geographical differentiation of the molecular diversity of cacao swollen shoot virus in Togo

Oro, Franck / Mississo, Essivi / Okassa, Mireille / Guilhaumon, Claire / Fenouillet, Catherine / Cilas, Christian / Muller, Emmanuelle

Archives of virology.. 2012 Mar., v. 157, no. 3

2012

Abstract: The variability of cacao swollen shoot virus (CSSV) was studied in two areas of Togo with distinct epidemiological backgrounds, Kloto and Litimé. This molecular study was based on 120 sequence comparisons of the first part of ORF3 of the viral genome. ... ...

Abstract	The variability of cacao swollen shoot virus (CSSV) was studied in two areas of Togo with distinct epidemiological backgrounds, Kloto and Litimé. This molecular study was based on 120 sequence comparisons of the first part of ORF3 of the viral genome. The phylogenetic study distinguished three groups of CSSV isolates, A, B, and C, with clear geographical differentiation between the Kloto and Litimé areas. The only group detected in Kloto was group C, whereas the Litimé area only contained isolates from groups A and B, which most probably originated from Ghana. The divergence between the three groups strongly suggests a different origin for each of the groups.
Keywords	Cacao swollen shoot virus ; genome ; phylogeny ; sequence analysis ; Ghana
Language	English
Dates of publication	2012-03
Size	p. 509-514.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-011-1158-x
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Article ; Online: Geographical differentiation of the molecular diversity of cacao swollen shoot virus in Togo.

Oro, Franck / Mississo, Essivi / Okassa, Mireille / Guilhaumon, Claire / Fenouillet, Catherine / Cilas, Christian / Muller, Emmanuelle

Archives of virology

2012 Volume 157, Issue 3, Page(s) 509–514

Abstract	The variability of cacao swollen shoot virus (CSSV) was studied in two areas of Togo with distinct epidemiological backgrounds, Kloto and Litimé. This molecular study was based on 120 sequence comparisons of the first part of ORF3 of the viral genome. The phylogenetic study distinguished three groups of CSSV isolates, A, B, and C, with clear geographical differentiation between the Kloto and Litimé areas. The only group detected in Kloto was group C, whereas the Litimé area only contained isolates from groups A and B, which most probably originated from Ghana. The divergence between the three groups strongly suggests a different origin for each of the groups.
MeSH term(s)	Badnavirus/classification ; Badnavirus/genetics ; Badnavirus/isolation & purification ; Cacao/virology ; Cluster Analysis ; DNA, Viral/genetics ; Genetic Variation ; Genotype ; Molecular Sequence Data ; Open Reading Frames ; Phylogeography ; Plant Diseases/virology ; Sequence Analysis, DNA ; Togo
Chemical Substances	DNA, Viral
Language	English
Publishing date	2012-03
Publishing country	Austria
Document type	Journal Article
ZDB-ID	7491-3
ISSN	1432-8798 ; 0304-8608
ISSN (online)	1432-8798
ISSN	0304-8608
DOI	10.1007/s00705-011-1158-x
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Article: Molecular and morphological boundaries of the predatory mite Neoseiulus californicus (McGregor) (Acari: Phytoseiidae)

OKASSA, MIREILLE / KREITER, SERGE / GUICHOU, SABINE / TIXIER, MARIE‐STÉPHANE

Biological journal of the Linnean Society. 2011 Oct., v. 104, no. 2

2011

Abstract: Neoseiulus californicus (McGregor) is a natural enemy of pest mites used worldwide in many crops. Its correct identification is thus essential to ensure biological control success. The present study aimed to characterize molecular and morphological ... ...

Abstract	Neoseiulus californicus (McGregor) is a natural enemy of pest mites used worldwide in many crops. Its correct identification is thus essential to ensure biological control success. The present study aimed to characterize molecular and morphological intraspecific variations for assisting in the diagnosis of the species and to build baseline information about expected variations within a commercially important phytoseiid species. Morphological and molecular [12S rRNA, cytochrome b mitochondrial (mt)DNA, and internal transcribed spacer] analyses were carried out on fourteen populations collected worldwide and on one mass‐reared strain. The genetic distances between the specimens of N. californicus and another related species were high and no overlap was observed, sustaining the reliability of such molecular methods for assisting a specific diagnosis. Furthermore, the genetic distances between populations of N. californicus were very low and overlap between intra‐ and interpopulations distances was observed, except for two populations collected in France (Marsillargues and Midi‐Pyrénées). The high mitochondrial differentiation between these two latter populations and the others questions their specific status: do they belong to the species N. californicus or to another cryptic species? However, using nuclear DNA marker analyses, no distinct differences were observed. Furthermore, even if significant morphological differences were observed between the populations, these differences were very small and the standard errors within each population were very low. We thus concluded that all the populations studied belong to the species N. californicus, despite unexpected high mitochondrial variations. The present study thus displays the importance of an integrative taxonomic approach for avoiding misidentifications. A discussion on morphological and mtDNA variations in relation to diagnostic reliability is developped. © 2011 The Linnean Society of London, Biological Journal of the Linnean Society, 2011, 104, 393–406.
Keywords	Neoseiulus californicus ; biological control ; crops ; cytochrome b ; internal transcribed spacers ; mitochondrial DNA ; nuclear genome ; pests ; predatory mites ; ribosomal RNA ; France
Language	English
Dates of publication	2011-10
Size	p. 393-406.
Publishing place	Blackwell Publishing Ltd
Document type	Article
ZDB-ID	1461865-5
ISSN	1095-8312 ; 0024-4066
ISSN (online)	1095-8312
ISSN	0024-4066
DOI	10.1111/j.1095-8312.2011.01717.x
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