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Article ; Online: Phospho-Tau 181 quantification method for Alzheimer's disease based on an array 2D biosensor combined with surface plasmon resonance imaging.

Oldak, Lukasz / Zielinska, Zuzanna / Socha, Katarzyna / Bogdan, Sylwia / Gorodkiewicz, Ewa

2024 Volume 271, Page(s) 125736

Abstract: Alzheimer's disease is among the neurodegenerative diseases for which there is a lack of rapid, effective, and non-invasive diagnostic methods. The development of a phospho-Tau 181 assay biosensor is therefore a response to the need for methods to ... ...

Abstract	Alzheimer's disease is among the neurodegenerative diseases for which there is a lack of rapid, effective, and non-invasive diagnostic methods. The development of a phospho-Tau 181 assay biosensor is therefore a response to the need for methods to diagnose AD. The present work was aimed at developing a fast, selective, and repeatable method for the quantitative determination of phospho-Tau 181, which could be used even during routine blood tests. Our method is a form of what is called liquid biopsy. The developed method underwent validation, as a result of which its analytical parameters were determined. An LOQ of 3.35 pg mL
MeSH term(s)	Humans ; Alzheimer Disease/diagnostic imaging ; tau Proteins ; Surface Plasmon Resonance/methods ; Biomarkers ; Biosensing Techniques ; Amyloid beta-Peptides
Chemical Substances	tau Proteins ; Biomarkers ; Amyloid beta-Peptides
Language	English
Publishing date	2024-02-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2024.125736
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Article ; Online: Comparison of Fluidic and Non-Fluidic Surface Plasmon Resonance Biosensor Variants for Angular and Intensity Modulation Measurements.

Mrozek, Piotr / Oldak, Lukasz / Gorodkiewicz, Ewa

Sensors (Basel, Switzerland)

2023 Volume 23, Issue 24

Abstract: Fluidic and non-fluidic surface plasmon resonance measurements were realized for the same type of sensory layer and using the same mouse IgG antibody and anti-mouse IgG antibody biomolecular system. A comparison of the thicknesses of the anti-mouse IgG ... ...

Abstract	Fluidic and non-fluidic surface plasmon resonance measurements were realized for the same type of sensory layer and using the same mouse IgG antibody and anti-mouse IgG antibody biomolecular system. A comparison of the thicknesses of the anti-mouse IgG antibody layers bound to the ligand at increasing analyte concentrations ranging from 0.0 μg mL
MeSH term(s)	Animals ; Mice ; Surface Plasmon Resonance/methods ; Biosensing Techniques/methods ; Immunoglobulin G ; Ligands
Chemical Substances	Immunoglobulin G ; Ligands
Language	English
Publishing date	2023-12-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s23249899
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Article ; Online: A New Approach to the Quantification of Fibroblast Growth Factor 23-An Array Surface Plasmon Resonance Imaging Biosensor.

Tokarzewicz, Anna / Ołdak, Łukasz / Młynarczyk, Grzegorz / Klekotka, Urszula / Gorodkiewicz, Ewa

International journal of molecular sciences

2023 Volume 24, Issue 20

Abstract: A new biosensor based on the "surface plasmon resonance imaging (SPRi)" detection technique for the quantification of "fibroblast growth factor 23 (FGF23)" has been developed. FGF23 is mainly produced in bone tissues as a phosphaturic hormone that forms ... ...

Abstract	A new biosensor based on the "surface plasmon resonance imaging (SPRi)" detection technique for the quantification of "fibroblast growth factor 23 (FGF23)" has been developed. FGF23 is mainly produced in bone tissues as a phosphaturic hormone that forms a trimeric complex with "fibroblast growth factor receptor 1 (FGFR1)" and αKlotho upon secretion. FGF23 stimulates phosphate excretion and inhibits the formation of active vitamin D in the kidneys. FGF23 has been shown to play a role in bone carcinogenesis and metastasis. The newly developed method, based on the array SPRi biosensor, was validated-the precision, accuracy, and selectivity were acceptable, and yielded less than ±10% recovery. The rectilinear response of the biosensor ranges from 1 to 75 pg/mL. The limit of detection was 0.033 pg/mL, and the limit of quantification was 0.107 pg/mL. The biosensor was used to determine FGF23 concentrations in the blood plasma of healthy subjects and patients with "clear cell" renal cell carcinoma (ccRCC). The obtained results were compared with those measured through an "enzyme-linked immunosorbent assay (ELISA)". The determined Pearson correlation coefficients were 0.994 and 0.989, demonstrating that the newly developed biosensor can be used as a competitive method for the ELISA.
MeSH term(s)	Humans ; Surface Plasmon Resonance ; Fibroblast Growth Factor-23 ; Biosensing Techniques/methods ; Phosphates/metabolism ; Enzyme-Linked Immunosorbent Assay ; Fibroblast Growth Factors/metabolism
Chemical Substances	Fibroblast Growth Factor-23 (7Q7P4S7RRE) ; Phosphates ; Fibroblast Growth Factors (62031-54-3)
Language	English
Publishing date	2023-10-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms242015327
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Article ; Online: Methods of PARP-1 Determination and its Importance in Living Organisms.

Zielińska, Zuzanna / Ołdak, Łukasz / Gorodkiewicz, Ewa

Protein and peptide letters

2022 Volume 29, Issue 6, Page(s) 496–504

Abstract: PARP-1 is one of the 18 PARP enzymes that are involved in important processes at the cellular level. The most important tasks of PARP-1 are to detect and repair DNA damage and to prevent processes of apoptosis. By finding and using new strategies for ... ...

Abstract	PARP-1 is one of the 18 PARP enzymes that are involved in important processes at the cellular level. The most important tasks of PARP-1 are to detect and repair DNA damage and to prevent processes of apoptosis. By finding and using new strategies for marking and detecting the activity of this protein, it is possible to identify more and more tasks in which it participates. In pathological states, PARP-1 activity increases significantly. Since the 1980s, scientists have been searching for and discussing substances that may inhibit PARP-1 activity and disrupt DNA damage response pathways. In this way, unwanted cells could be destroyed. The paper presents a short description of the methods used in the determination of PARP-1 by various research groups. A critical approach to each of them was also made by pointing to the advantages and disadvantages of the described analytical methods. The literature review contains information on methods useful for PARP-1 determination, such as SPR, QCM, CL and FL, DPV, SDS-PAGE with MS, MALDI MS, Western Blot, ELISA and ATR-FTIR spectroscopy. It also includes analysis of the results of research on inhibitors that may be effective in the diagnosis and treatment of cancer and other diseases.
MeSH term(s)	Apoptosis ; DNA Damage ; DNA Repair ; Humans ; Neoplasms/drug therapy ; Poly(ADP-ribose) Polymerase Inhibitors/pharmacology ; Poly(ADP-ribose) Polymerase Inhibitors/therapeutic use
Chemical Substances	Poly(ADP-ribose) Polymerase Inhibitors
Language	English
Publishing date	2022-04-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1280776-x
ISSN	1875-5305 ; 0929-8665
ISSN (online)	1875-5305
ISSN	0929-8665
DOI	10.2174/0929866529666220405160715
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Article ; Online: Studies of interactions between fibronectin and a specific antibody against fibronectin using SPRi and QCM.

Oldak, Lukasz / Lukaszewski, Zenon / Gorodkiewicz, Ewa

Journal of pharmaceutical and biomedical analysis

2022 Volume 212, Page(s) 114640

Abstract: Surface Plasmon Resonance Imaging (SPRi) and the Quartz Crystal Microbalance (QCM) technique were used to characterize the interactions between fibronectin and a specific monoclonal antibody against fibronectin. These techniques were used to investigate ... ...

Abstract	Surface Plasmon Resonance Imaging (SPRi) and the Quartz Crystal Microbalance (QCM) technique were used to characterize the interactions between fibronectin and a specific monoclonal antibody against fibronectin. These techniques were used to investigate the formation of successive layers of the biosensor and how they affect the biosensor's response. The analytical response of both detectors to fibronectin concentration is linear in the range 5-100 ng/mL. The changes in mass on the surface of a quartz crystal covered with a layer of gold during biosensor formation were investigated with a QCM. The equilibrium constant was determined to be K
MeSH term(s)	Biosensing Techniques/methods ; Fibronectins ; Gold/chemistry ; Quartz Crystal Microbalance Techniques/methods ; Surface Plasmon Resonance
Chemical Substances	Fibronectins ; Gold (7440-57-5)
Language	English
Publishing date	2022-02-01
Publishing country	England
Document type	Journal Article
ZDB-ID	604917-5
ISSN	1873-264X ; 0731-7085
ISSN (online)	1873-264X
ISSN	0731-7085
DOI	10.1016/j.jpba.2022.114640
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Article ; Online: Development of an SPRi Test for the Quantitative Detection of Cadherin 12 in Human Plasma and Peritoneal Fluid.

Oldak, Lukasz / Lukaszewski, Zenon / Leśniewska, Anna / Goławski, Ksawery / Laudański, Piotr / Gorodkiewicz, Ewa

International journal of molecular sciences

2023 Volume 24, Issue 23

Abstract: A new method for the determination of cadherin 12 (CDH12)-an adhesive protein that has a significant impact on the development, growth, and movement of cancer cells-was developed and validated. The method is based on a biosensor using surface plasmon ... ...

Abstract	A new method for the determination of cadherin 12 (CDH12)-an adhesive protein that has a significant impact on the development, growth, and movement of cancer cells-was developed and validated. The method is based on a biosensor using surface plasmon resonance imaging (SPRi) detection. A quartz crystal microbalance was used to analyze the characteristics of the formation of successive layers of the biosensor, from the linker monolayer to the final capture of CDH12 from solution. The association equilibrium constant (K
MeSH term(s)	Humans ; Surface Plasmon Resonance/methods ; Ascitic Fluid ; Biosensing Techniques/methods ; Cadherins
Chemical Substances	Cadherins
Language	English
Publishing date	2023-11-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms242316894
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Article ; Online: An Immunosensor for the Determination of Cathepsin S in Blood Plasma by Array SPRi-A Comparison of Analytical Properties of Silver-Gold and Pure Gold Chips.

Falkowski, Pawel / Mrozek, Piotr / Lukaszewski, Zenon / Oldak, Lukasz / Gorodkiewicz, Ewa

Biosensors

2021 Volume 11, Issue 9

Abstract: The array SPR imaging (SPRi) technique is well suited to the determination of biomarkers in body fluids, called liquid biopsy. No signal enhancement or analyte preconcentration is required. With the aim of achieving signal enhancement and lowering the ... ...

Abstract	The array SPR imaging (SPRi) technique is well suited to the determination of biomarkers in body fluids, called liquid biopsy. No signal enhancement or analyte preconcentration is required. With the aim of achieving signal enhancement and lowering the cost of a single determination, the replacement of gold-covered chips by silver-gold chips was investigated. The aim of this work was to investigate the analytical characteristics of a biosensor formed on a Ag/Au chip and to compare them with those of a biosensor formed on a gold chip. A biosensor for the determination of cathepsin S (Cath S) was chosen as an example. The biosensor consisted of the linker cysteamine and an immobilized rat monoclonal antibody specific for cathepsin S. Both biosensors exhibited a Langmuirian response to Cath S concentration, with linear response ranging from LOQ to 1.5 ng mL
MeSH term(s)	Animals ; Biosensing Techniques ; Cathepsins/blood ; Female ; Gold ; Humans ; Immunoassay ; Ovarian Neoplasms ; Plasma ; Rats ; Silver ; Surface Plasmon Resonance
Chemical Substances	Silver (3M4G523W1G) ; Gold (7440-57-5) ; Cathepsins (EC 3.4.-) ; cathepsin S (EC 3.4.22.27)
Language	English
Publishing date	2021-08-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios11090298
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Article ; Online: Two Biosensors for the Determination of Interleukin-6 in Blood Plasma by Array SPRi.

Szymanska, Beata / Lukaszewski, Zenon / Oldak, Lukasz / Zelazowska-Rutkowska, Beata / Hermanowicz-Szamatowicz, Kinga / Gorodkiewicz, Ewa

Biosensors

2022 Volume 12, Issue 6

Abstract: Interleukin-6 (IL-6) is a biomarker of inflammation, the advanced stage of COVID-19, and several cancers, including ovarian cancer. Two biosensors for the determination of IL-6 in blood plasma by array SPRi have been developed. One of these biosensors ... ...

Abstract	Interleukin-6 (IL-6) is a biomarker of inflammation, the advanced stage of COVID-19, and several cancers, including ovarian cancer. Two biosensors for the determination of IL-6 in blood plasma by array SPRi have been developed. One of these biosensors consists of the mouse monoclonal anti-IL-6 antibody as the receptor immobilized via the cysteamine linker. The second contains galiellalactone as the receptor, being an inhibitor specific for IL-6, immobilized via octadecanethiol (ODM) as the linker. Both biosensors are specific for IL-6. The biosensor with the antibody as the receptor gives a linear analytical response between 3 (LOQ) and 20 pg mL
MeSH term(s)	Animals ; Biosensing Techniques ; COVID-19 ; Female ; Humans ; Interleukin-6 ; Mice ; Ovarian Neoplasms ; Plasma
Chemical Substances	Interleukin-6
Language	English
Publishing date	2022-06-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios12060412
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Article ; Online: MMP-1, UCH-L1, and 20S Proteasome as Potential Biomarkers Supporting the Diagnosis of Brain Glioma.

Oldak, Lukasz / Chludzinska-Kasperuk, Sylwia / Milewska, Patrycja / Grubczak, Kamil / Reszec, Joanna / Gorodkiewicz, Ewa

Biomolecules

2022 Volume 12, Issue 10

Abstract: The diagnosis of brain gliomas is mainly based on imaging methods. The gold standard in this area is MRI. Recommendations for the prevention, diagnosis, and treatment of gliomas are periodically modified and updated. One of the diagnostic techniques used ...

Abstract	The diagnosis of brain gliomas is mainly based on imaging methods. The gold standard in this area is MRI. Recommendations for the prevention, diagnosis, and treatment of gliomas are periodically modified and updated. One of the diagnostic techniques used when a brain glioma is suspected is liquid biopsy. However, this technique requires further development to confirm its effectiveness. This paper presents a proposal of three potential biomarkers of brain gliomas-extracellular matrix metalloproteinase-1 (MMP-1), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), and the 20S proteasome-which were quantified in blood plasma using SPRi biosensors. A statistical analysis of the results indicated no significant changes in the concentrations between the control group (K) and grades G1 and G2, and similarly between grades G3 and G4. However, the differences in the concentrations between the groups K/G1/G2 and G3/G4 were statistically significant. A positive average correlation was found between the concentrations of the proteins and the patient's age. The individual tested proteins were also highly correlated with each other. Our work proposes a new diagnostic technique that may aid in the diagnosis of brain gliomas.
MeSH term(s)	Humans ; Ubiquitin Thiolesterase/metabolism ; Proteasome Endopeptidase Complex/metabolism ; Matrix Metalloproteinase 1/metabolism ; Glioma/diagnosis ; Glioma/pathology ; Biomarkers ; Brain/metabolism
Chemical Substances	Ubiquitin Thiolesterase (EC 3.4.19.12) ; Proteasome Endopeptidase Complex (EC 3.4.25.1) ; Matrix Metalloproteinase 1 (EC 3.4.24.7) ; Biomarkers
Language	English
Publishing date	2022-10-13
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2701262-1
ISSN	2218-273X ; 2218-273X
ISSN (online)	2218-273X
ISSN	2218-273X
DOI	10.3390/biom12101477
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Article: Cathepsin B, D and S as Potential Biomarkers of Brain Glioma Malignancy.

Oldak, Lukasz / Milewska, Patrycja / Chludzinska-Kasperuk, Sylwia / Grubczak, Kamil / Reszec, Joanna / Gorodkiewicz, Ewa

Journal of clinical medicine

2022 Volume 11, Issue 22

Abstract: Brain gliomas constitute the vast majority of malignant tumors of the nervous system. There is still a lack of fast, reliable and non-invasive methods of diagnostics. Our work focuses on the quantification of cathepsin B, D and S in glioma. The research ... ...

Abstract	Brain gliomas constitute the vast majority of malignant tumors of the nervous system. There is still a lack of fast, reliable and non-invasive methods of diagnostics. Our work focuses on the quantification of cathepsin B, D and S in glioma. The research was conducted with the use of SPRi biosensors sensitive to individual cathepsins. Changes in the quantity of selected cathepsins (cathepsins B, D and S), depending on the advancement of glioma and the presence or absence of important features or comorbidities in the selected patient, were examined. The results were statistically analyzed and interpreted based on the available clinical description. Statistical significance was observed in the difference in the concentration of the studied cathepsins, mainly between the groups Control and G3/G4 and G1/G2 and G3/G4. The strength of the correlation between the concentrations of individual cathepsins and the age of the patient and the size of the tumor, as well as the correlation between individual proteins, was investigated. The influence of IDH 1/2 status on the concentration of determined cathepsins was investigated and ROC analysis was performed. As a result of our research, we have developed a method for the diagnosis of brain glioma that allows us to distinguish grades G1/G2 from G3/G4 and the control group from G3/G4. We found an average positive correlation between the concentrations of the proteins tested and the age of the patient and a high positive correlation between the cathepsins tested. Comparative analysis of the effect of the presence of IDH 1/2 mutations on the number of proteins tested allowed us to demonstrate that the cathepsins assayed can be independent markers.
Language	English
Publishing date	2022-11-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm11226763
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