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Article ; Online: Detection of Equus caballus Papillomavirus in Equine Aural Plaque Samples

Bromberger, Cristiana R. / Costa, Julia Rodrigues / Herman, Mariana / Hernandez, Juliana Mira / Albertino, Lukas Garrido / Alves, Carlos Eduardo Fonseca / Borges, Alexandre S. / Oliveira-Filho, Jose P.

Journal of Equine Veterinary Science. 2023 Sept., v. 128 p.104877-

2023

Abstract: Aural plaques have been linked to Equus caballus papillomavirus (EcPV). Ten types of EcPVs have already been described; however, only EcPVs 1, 3, 4, 5, and 6 have been observed in association with aural plaques. Accordingly, the objective of this study ... ...

Abstract	Aural plaques have been linked to Equus caballus papillomavirus (EcPV). Ten types of EcPVs have already been described; however, only EcPVs 1, 3, 4, 5, and 6 have been observed in association with aural plaques. Accordingly, the objective of this study was to evaluate the presence of EcPVs in equine aural plaque samples. A total of 29 aural plaque samples (from 15 horses) were collected and assessed for the presence of the DNA of these EcPVs by PCR. Additionally, 108 aural plaque samples used in previous research were evaluated for the presence of EcPVs 8 and 9. Previously described primers were used for PCR to detect EcPVs 1 to 8, and specific primers were designed for EcPV 9. Minigenes were synthesized and used as a positive control in the PCRs for the undetected EcPVs. EcPVs 2, 7, 8, and 9 were not detected in any of the evaluated samples, suggesting that these viral types are not involved in the etiology of the equine aural plaque in Brazil. EcPV 6 was the most prevalent (81%), followed by EcPVs 3 (72%), 4 (63%) and 5 (47%), which reinforces the idea that these viruses play an important role in the etiology of the equine aural plaque in Brazil.
Keywords	DNA ; Zetapapillomavirus 1 ; etiology ; horses ; veterinary medicine ; Brazil ; Aural plaques ; Horse ; Papillomavirus ; PCR ; Skin
Language	English
Dates of publication	2023-09
Publishing place	Elsevier Inc.
Document type	Article ; Online
ZDB-ID	2102631-2
ISSN	1542-7412 ; 0737-0806
ISSN (online)	1542-7412
ISSN	0737-0806
DOI	10.1016/j.jevs.2023.104877
Database	NAL-Catalogue (AGRICOLA)

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Article: Cerebrospinal fluid analysis in horses, cattle, and sheep diagnosed with rabies: A retrospective study of 62 cases.

Zakia, Luiza S / Albertino, Lukas G / Andrade, Danilo G A / Amorim, Rogerio M / Takahira, Regina R / Oliveira-Filho, Jose P / Borges, Alexandre S

The Canadian veterinary journal = La revue veterinaire canadienne

2022 Volume 63, Issue 12, Page(s) 1242–1246

Abstract: Objectives: This study aimed to characterize the findings in cerebral spinal fluid (CSF) analysis of horses, cattle, and sheep diagnosed with rabies.: Animals: The study included 62 animals (horses, cattle, and sheep) diagnosed with rabies at a ... ...

Abstract	Objectives: This study aimed to characterize the findings in cerebral spinal fluid (CSF) analysis of horses, cattle, and sheep diagnosed with rabies. Animals: The study included 62 animals (horses, cattle, and sheep) diagnosed with rabies at a referral hospital. Methods: This was a retrospective study using medical records from large animals with neurological signs and confirmed positive direct immunofluorescence test for rabies from 2003 to 2020. The results of CSF analysis are presented descriptively. Results: Cerebral spinal fluid samples (N = 67) from 62 animals (31 horses, 24 cattle, and 7 sheep) were retrospectively evaluated. Of these 3 species, 28% (19/67) showed increased protein concentration, whereas 58% (39/67) presented mononuclear pleocytosis. In total, 37% of the samples (25/67) had protein concentration and total nucleated cell count within the reference range. Conclusions and clinical relevance: Cerebral spinal fluid from animals diagnosed with rabies was either normal or characterized by mild mononuclear pleocytosis and hyperproteinorrachia.
MeSH term(s)	Sheep ; Cattle ; Horses ; Animals ; Retrospective Studies ; Rabies/diagnosis ; Rabies/veterinary ; Leukocytosis/veterinary ; Medical Records ; Reference Values ; Cattle Diseases/diagnosis ; Horse Diseases/diagnosis ; Sheep Diseases/diagnosis
Language	English
Publishing date	2022-11-22
Publishing country	Canada
Document type	Journal Article
ZDB-ID	41603-4
ISSN	0008-5286
ISSN	0008-5286
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Article ; Online: Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia.

Scavone, Donatella / Sgorbini, Micaela / Borges, Alexandre S / Oliveira-Filho, José P / Vitale, Valentina / Paltrinieri, Saverio

BMC veterinary research

2020 Volume 16, Issue 1, Page(s) 422

Abstract: Background: Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but ... ...

Abstract	Background: Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but promising findings about its utility in diagnosing SIRS and predicting the outcome in diseased horses, were recently highlighted. The objective of the study was to investigate the behaviour of PON-1 in horses after experimentally induced endotoxemia. To this aim, PON-1 activity was measured on 66 plasma samples collected from six clinically healthy mares, previously included in another study, before and at multiple time points between 12 and 240 h after intravenous infusion of Escherichia coli O55:B5 lipopolysaccharide (LPS). Results: Compared with baseline values, a progressive transient decrease of PON-1 activity was observed starting from 24 h post-infusion, with lowest values observed between 3 to 7 days post-infusion, followed by a normalisation to pre-infusion levels the tenth day. Conclusions: The results of this study suggest that measurement and monitoring of PON-1 activity might be useful to evaluate progression and recovery from endotoxemia in horses. Further studies in horses with naturally occurring sepsis are warranted.
MeSH term(s)	Animals ; Aryldialkylphosphatase/blood ; Endotoxemia/blood ; Endotoxemia/chemically induced ; Endotoxemia/diagnosis ; Endotoxemia/enzymology ; Escherichia coli ; Female ; Horse Diseases/blood ; Horse Diseases/diagnosis ; Horse Diseases/enzymology ; Horses ; Lipopolysaccharides/administration & dosage
Chemical Substances	Lipopolysaccharides ; Aryldialkylphosphatase (EC 3.1.8.1)
Language	English
Publishing date	2020-11-04
Publishing country	England
Document type	Journal Article
ISSN	1746-6148
ISSN (online)	1746-6148
DOI	10.1186/s12917-020-02629-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Detection of Equus caballus Papillomavirus in Equine Aural Plaque Samples.

Bromberger, Cristiana R / Costa, Julia Rodrigues / Herman, Mariana / Hernandez, Juliana Mira / Albertino, Lukas Garrido / Alves, Carlos Eduardo Fonseca / Borges, Alexandre S / Oliveira-Filho, Jose P

Journal of equine veterinary science

2023 Volume 128, Page(s) 104877

Abstract	Aural plaques have been linked to Equus caballus papillomavirus (EcPV). Ten types of EcPVs have already been described; however, only EcPVs 1, 3, 4, 5, and 6 have been observed in association with aural plaques. Accordingly, the objective of this study was to evaluate the presence of EcPVs in equine aural plaque samples. A total of 29 aural plaque samples (from 15 horses) were collected and assessed for the presence of the DNA of these EcPVs by PCR. Additionally, 108 aural plaque samples used in previous research were evaluated for the presence of EcPVs 8 and 9. Previously described primers were used for PCR to detect EcPVs 1 to 8, and specific primers were designed for EcPV 9. Minigenes were synthesized and used as a positive control in the PCRs for the undetected EcPVs. EcPVs 2, 7, 8, and 9 were not detected in any of the evaluated samples, suggesting that these viral types are not involved in the etiology of the equine aural plaque in Brazil. EcPV 6 was the most prevalent (81%), followed by EcPVs 3 (72%), 4 (63%) and 5 (47%), which reinforces the idea that these viruses play an important role in the etiology of the equine aural plaque in Brazil.
MeSH term(s)	Horses/genetics ; Animals ; Polymerase Chain Reaction/veterinary ; Papillomaviridae/genetics ; Brazil
Language	English
Publishing date	2023-07-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2102631-2
ISSN	1542-7412 ; 0737-0806
ISSN (online)	1542-7412
ISSN	0737-0806
DOI	10.1016/j.jevs.2023.104877
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: DMRT3

Herman, Mariana / Caceres, Amanda Manara / Albuquerque, Ana Luísa H / Leite, Raíssa O / Araújo, César Erineudo T / Delfiol, Diego José Z / Curi, Rogério A / Borges, Alexandre S / Oliveira-Filho, José P

Animals : an open access journal from MDPI

2023 Volume 13, Issue 24

Abstract: In Brazil, the production of mules with a comfortable gait primarily involves the breeding of marching saddle mules. This is achieved by crossing gaited Pêga donkeys with horses from the Mangalarga Marchador and Campolina breeds. ... ...

Abstract	In Brazil, the production of mules with a comfortable gait primarily involves the breeding of marching saddle mules. This is achieved by crossing gaited Pêga donkeys with horses from the Mangalarga Marchador and Campolina breeds. The
Language	English
Publishing date	2023-12-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani13243829
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A complex

Chimenes, Natielly D / Caramalac, Silvana M / Caramalac, Simone M / Fernandes, Thiago D / Basso, Roberta M / Cerri, Fabrício M / Oliveira-Filho, José P / Borges, Alexandre S / Palumbo, Mariana I P

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

2023 Volume 35, Issue 4, Page(s) 413–416

Abstract: Hereditary myotonia (HM) is characterized by delayed muscle relaxation after contraction as a result of a mutation in ... ...

Abstract	Hereditary myotonia (HM) is characterized by delayed muscle relaxation after contraction as a result of a mutation in the
MeSH term(s)	Animals ; Dogs ; Male ; Chloride Channels/genetics ; Chloride Channels/metabolism ; Dog Diseases/diagnosis ; Dog Diseases/genetics ; Exons ; Mutation ; Myotonia/genetics ; Myotonia/veterinary ; Myotonia Congenita/diagnosis ; Myotonia Congenita/genetics ; Myotonia Congenita/veterinary
Chemical Substances	Chloride Channels
Language	English
Publishing date	2023-05-22
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/10406387231176736
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Equine blastocyst re-expansion rate, quality, and sex following embryonic collapse using needle perforation of blastocoel and subsequent PCR amplification of blastocoel DNA

Camargo, Giovana S / Meira, Cezinande / Bergfelt, Don R / Oliveira-Filho, José P. / Bromberger, Cristiana R. / Barros, Luiz D. / Canesin, Heloisa S / Lopes, Giulia VR / Monteiro, Gabriel A / Ignácio, Fernanda S

Journal of Equine Veterinary Science. 2023 June, v. 125 p.104633-

2023

Abstract: Collapse of equine blastocysts following needle perforation of the blastocoel prior to vitrification (Ferris et al., Journal of Equine Veterinary Science. 2016; 100:64-65) and collection and amplification of the free DNA blastocoel fluid may be used for ... ...

Abstract	Collapse of equine blastocysts following needle perforation of the blastocoel prior to vitrification (Ferris et al., Journal of Equine Veterinary Science. 2016; 100:64-65) and collection and amplification of the free DNA blastocoel fluid may be used for sexing embryos (Herrera et al., Theriogenology. 2015; 83:415-420). The present study aimed to evaluate equine blastocyst re-expansion rate, quality, and sex following manual perforation of the blastocoel and PCR amplification of blastocoel fluid DNA. Embryos (n=19) were collected on day 8 after ovulation, assessed initially and transferred to a 50µL droplet of holding medium in which the blastocoel was manually perforated with a 26g hypodermic needle. Within one minute of detecting a diameter decrease or collapse, the entire volume of each droplet of medium was collected and stored at -20°C until PCR. Some of the collapsed embryos (n=11) were moved and immersed in 2mL of holding medium and cultured in isothermal boxes at 25°C. After 24h, embryo size and quality were reassessed. Post-culture, collapsed blastocysts had partially re-expanded to their initial diameter with a mean expansion rate of 68.8%. Quality of re-expanded embryos was Grade 2 (good, 6/11), Grade 3 (poor, 4/11), and Grade 4 (degenerated, 1/11) (McKinnon,Squires, J. Am. Vet Med. Assoc. 1988; 192:401-406). Non-perforated embryos (n=10) used as controls were initially Grade 1 (excellent, 8/10) and Grade 2 (2/10) and, after 24h of culture, Grade 1 (9/10) and Grade 2 (1/10) with a 25.9% growth rate. Blastocoel fluid from all collapsed embryos (n=19) was subjected to double PCR amplification of the male TSPY gene with blood of an adult male and female horse used as controls. Gene amplification indicated 57.9% (11/19) of embryos were male and non-amplification indicated 31.6% (6/19) of embryos were female. Gene amplification was inconclusive for 10.5% (2/19) of the embryos even though they were completely collapsed. Thus, needle-induced leakage and collapse of the blastocoel resulted in a high rate of blastocysts re-expansion (69%) with many embryos (54%) achieving good quality and potential for transfer as either male or female embryos. In summary, while the number of potentially transferable embryos that recovered from collapse was less than in the control group, the success of these preliminary results warrants further development to decrease damage and increase the culture re-expansion rate and quality of transferable male or female embryos for field application in an equine embryo transfer program.
Keywords	DNA ; adults ; blastocyst ; blood ; droplets ; embryo transfer ; females ; gene amplification ; genes ; horses ; males ; ovulation ; veterinary medicine ; vitrification
Language	English
Dates of publication	2023-06
Publishing place	Elsevier Inc.
Document type	Article ; Online
ZDB-ID	2102631-2
ISSN	1542-7412 ; 0737-0806
ISSN (online)	1542-7412
ISSN	0737-0806
DOI	10.1016/j.jevs.2023.104633
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A complex CLCN1 variant associated with hereditary myotonia in a mixed-breed dog

Chimenes, Natielly D. / Caramalac, Silvana M. / Caramalac, Simone M. / Fernandes, Thiago D. / Basso, Roberta M. / Cerri, Fabrício M. / Oliveira-Filho, José P. / Borges, Alexandre S. / Palumbo, Mariana I. P.

Journal of Veterinary Diagnostic Investigation. 2023 July, v. 35, no. 4 p.413-416

2023

Abstract: Hereditary myotonia (HM) is characterized by delayed muscle relaxation after contraction as a result of a mutation in the CLCN1 gene. We describe here a complex CLCN1 variant in a mixed-breed dog with clinical and electromyographic signs of HM. Blood ... ...

Abstract	Hereditary myotonia (HM) is characterized by delayed muscle relaxation after contraction as a result of a mutation in the CLCN1 gene. We describe here a complex CLCN1 variant in a mixed-breed dog with clinical and electromyographic signs of HM. Blood samples from the myotonic dog, as well as from his male littermate and parents, were analyzed via amplification of the 23 exons encoding CLCN1. After sequencing the CLCN1 gene, a complex variant was found in exon 6 c.[705T>G; 708del; 712_732del], resulting in a premature stop codon in exon 7 and a protein that was 717 amino acids shorter than the normal CLC protein. The myotonic dog was identified as homozygous recessive for the complex CLCN1 variant; its parents were heterozygous, and its male littermate was homozygous wild-type. Knowledge of the CLCN1 mutations responsible for the development of hereditary myotonia allows greater clarification of this condition.
Keywords	blood ; dogs ; electromyography ; exons ; heterozygosity ; homozygosity ; males ; mixed breeds ; muscles ; mutation ; stop codon ; chloride channel ; genetic analyses ; hereditary ; myopathy
Language	English
Dates of publication	2023-07
Size	p. 413-416.
Publishing place	SAGE Publications
Document type	Article ; Online
ZDB-ID	287603-6
ISSN	1943-4936 ; 1040-6387
ISSN (online)	1943-4936
ISSN	1040-6387
DOI	10.1177/10406387231176736
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 3645: Show issues

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ab Jg. 2022: Lesesaal (EG)

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Article: Aggrecan, IL-1β, IL-6, and TNF-α profiles in the Articular Cartilage of Miniature Horses with Chondrodysplastic Dwarfism.

Basso, Roberta M / Andrade, Danilo G A / Alves, Carlos E F / Laufer-Amorim, Renée / Borges, Alexandre S / Oliveira-Filho, Jose P

Journal of equine veterinary science

2021 Volume 103, Page(s) 103643

Abstract: Dwarfism is a skeletal disorder that causes abnormal growth. In Miniature horses, dwarfism can occur as chondrodysplastic dwarfism, an autosomal recessive disorder associated with five mutations (D1, D2, D3*, D4 and c.6465A > T variant) in the aggrecan ( ... ...

Abstract	Dwarfism is a skeletal disorder that causes abnormal growth. In Miniature horses, dwarfism can occur as chondrodysplastic dwarfism, an autosomal recessive disorder associated with five mutations (D1, D2, D3*, D4 and c.6465A > T variant) in the aggrecan (ACAN) gene. The aim of this study was to evaluate the expression of aggrecan (at the gene and protein level) and specific cytokines (IL-1β, IL-6, and TNF-α) in the articular cartilage of Miniature horses with chondrodysplastic dwarfism (D4/c.6465A > T genotype). Metatarsal bone samples from eight dwarf Miniature horses were collected for histopathological analysis, and articular cartilage was collected to detect and quantify aggrecan levels through Western blotting and determine the relative expression levels of ACAN, IL-1β, IL-6, and TNF-α through qPCR. All affected animals presented chondrodysplasia-like lesions with disorganization of the chondrocyte layers and reduced the amount of an extracellular matrix. No significant difference in aggrecan expression levels in uncleaved samples from the dwarf and control groups (composed of phenotypically normal animals of similar age and breed (P = .7143)) was found using Western blotting. qPCR revealed that ACAN gene expression was higher in the affected animals than in normal animals (P = .0119). No significant difference in cytokine levels was detected between the groups. Mutant aggrecan may interfere with normal cellular function, leading to chondrodysplasia and the observed phenotypic findings.
MeSH term(s)	Aggrecans/genetics ; Animals ; Cartilage, Articular ; Dwarfism/genetics ; Dwarfism/veterinary ; Horse Diseases/genetics ; Horses ; Interleukin-6/genetics ; Tumor Necrosis Factor-alpha/genetics
Chemical Substances	Aggrecans ; Interleukin-6 ; Tumor Necrosis Factor-alpha
Language	English
Publishing date	2021-04-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2102631-2
ISSN	1542-7412 ; 0737-0806
ISSN (online)	1542-7412
ISSN	0737-0806
DOI	10.1016/j.jevs.2021.103643
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Serial measurements of Paraoxonase-1 (PON-1) activity in horses with experimentally induced endotoxemia

Scavone, Donatella / Sgorbini, Micaela / Borges, Alexandre S / Oliveira-Filho, José P / Vitale, Valentina / Paltrinieri, Saverio

BMC veterinary research. 2020 Dec., v. 16, no. 1

2020

Abstract: BACKGROUND: Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but ... ...

Abstract	BACKGROUND: Paraoxonase-1 (PON-1) is an antioxidant enzyme, whose activity decreases during the acute phase response in many species. Little is known about PON-1 and its role as a negative acute phase protein during septic inflammation in horses, but promising findings about its utility in diagnosing SIRS and predicting the outcome in diseased horses, were recently highlighted. The objective of the study was to investigate the behaviour of PON-1 in horses after experimentally induced endotoxemia. To this aim, PON-1 activity was measured on 66 plasma samples collected from six clinically healthy mares, previously included in another study, before and at multiple time points between 12 and 240 h after intravenous infusion of Escherichia coli O55:B5 lipopolysaccharide (LPS). RESULTS: Compared with baseline values, a progressive transient decrease of PON-1 activity was observed starting from 24 h post-infusion, with lowest values observed between 3 to 7 days post-infusion, followed by a normalisation to pre-infusion levels the tenth day. CONCLUSIONS: The results of this study suggest that measurement and monitoring of PON-1 activity might be useful to evaluate progression and recovery from endotoxemia in horses. Further studies in horses with naturally occurring sepsis are warranted.
Keywords	Escherichia coli ; acute phase proteins ; antioxidant enzymes ; behavior ; endotoxemia ; inflammation ; intravenous injection ; lipopolysaccharides ; mares ; measurement ; monitoring ; normal values ; prediction ; sampling ; veterinary medicine
Language	English
Dates of publication	2020-12
Size	p. 422.
Publishing place	BioMed Central
Document type	Article
Note	NAL-light
ISSN	1746-6148
DOI	10.1186/s12917-020-02629-4
Database	NAL-Catalogue (AGRICOLA)

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