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Article ; Online: Endoscopic Application of Purastat® in the Treatment of Solitary Rectal Ulcer Syndrome.

Gagliardi, Mario / Sica, Mariano / Oliviero, Giovanni / Maurano, Attilio / Zulli, Claudio

Journal of gastrointestinal and liver diseases : JGLD

2021 Volume 30, Issue 3, Page(s) 324

MeSH term(s)	Endoscopy ; Female ; Humans ; Middle Aged ; Rectal Diseases/diagnosis ; Rectal Diseases/drug therapy ; Ulcer/diagnosis ; Ulcer/drug therapy
Language	English
Publishing date	2021-09-21
Publishing country	Romania
Document type	Case Reports ; Journal Article
ZDB-ID	2427021-0
ISSN	1842-1121 ; 1841-8724
ISSN (online)	1842-1121
ISSN	1841-8724
DOI	10.15403/jgld-3688
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Article ; Online: Endoscopic Application of Purastat® in the Treatment of Solitary Rectal Ulcer Syndrome.

Gagliardi, Mario / Sica, Mariano / Oliviero, Giovanni / Maurano, Attilio / Zulli, Claudio

Journal of gastrointestinal and liver diseases : JGLD

2021

Abstract: A 49-years-old woman with a recent history of tenesmus, constipation, abdominal and rectal pain referred to our Unit. There was no previous history of rectal bleeding or a family history of gastrointestinal diseases. Laboratory tests showed haemoglobin ... ...

Abstract	A 49-years-old woman with a recent history of tenesmus, constipation, abdominal and rectal pain referred to our Unit. There was no previous history of rectal bleeding or a family history of gastrointestinal diseases. Laboratory tests showed haemoglobin 10.9 g/dl, MCV 72fl, ferritin 18 U/l. Physical examination was normal. The patient underwent a colonoscopy which identified a single ulcer of 2.5 cm of diameter without bleeding signs in the distal rectum, 4 cm from the anal margin. Histopathological examination revealed shallow ulceration with fibrosis in lamina propria. We decided to treat the ulcer with one application of Purastat. Four weeks later, a follow-up colonoscopy revealed a complete mucosal healing with only mild residual mucosal erythema. Solitary Rectal Ulcer Syndrome (SRUS) is an uncommon benign proctologic disease usually affecting young adults with a prevalence of 1:100.000 per year, equally affecting women and men with a slight predominance in young women. SRUS is characterized by chronic rectal pain and bleeding, constipation, incomplete evacuation, tenesmus and mucous discharge impairing the patients' quality of life. In past years several topical agents have been used reporting clinical improvements, also if none of them has been evaluated in prospective controlled trials. Purastat (3D-Matrix Europe SAS, France) is a novel self-assembling peptide developed as a haemostatic agent for endoscopic and surgical procedures. In addition to the known haemostatic effect, it has been hypothesized that the activated Purastat nanostructure favors the cell and tissue proliferative process since the similarity of the activated Purastat 3-D nanostructure with the natural extracellular matrix (ECM-SM) scaffold material would result in an adequate adherence of cells and regenerative tissues, achieving more effective healing of the mucosa. Based on this hypothetical re-epithelizing property of Purastat, we decided to use it in this patient, achieving mucosal healing and symptoms improvement.
Language	English
Publishing date	2021-08-10
Publishing country	Romania
Document type	Journal Article
ZDB-ID	2427021-0
ISSN	1842-1121 ; 1841-8724
ISSN (online)	1842-1121
ISSN	1841-8724
DOI	10.15403/jgld-3680
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Article ; Online: One-Day versus Three-Day Low-Residue Diet and Bowel Preparation Quality before Colonoscopy: A Multicenter, Randomized, Controlled Trial.

Scaglione, Giuseppe / Oliviero, Giovanni / Labianca, Orazio / Bianco, Maria Antonia / Granata, Rocco / Ruggiero, Luigi / Iovino, Paola

Digestive diseases (Basel, Switzerland)

2023 Volume 41, Issue 5, Page(s) 708–718

Abstract: Background: One-day low-residue diet (LRD) is recommended before colonoscopy, but only three single-center trials compared the 1-day versus 3-day LRD. The aim of this multicenter study was to compare the impact of a 3-day versus 1-day LRD on its ability ...

Abstract	Background: One-day low-residue diet (LRD) is recommended before colonoscopy, but only three single-center trials compared the 1-day versus 3-day LRD. The aim of this multicenter study was to compare the impact of a 3-day versus 1-day LRD on its ability to adequately and successfully prepare the bowel of outpatients that require a colonoscopy. The outpatients' tolerance and adherence to the LRD were also considered. Methods: Consecutive outpatients were randomized to 1-day versus 3-day LRD at three open-access endoscopy units. The primary endpoint consisted of the proportion of patients with a satisfactory degree of bowel cleanliness (Score 2-3 on the Boston Bowel Preparation Scale [BBPS] in each segment). Secondary endpoints were patients' tolerance and adherence to the prescribed diet evaluated by a standardized questionnaire. Results: 289 patients were included in the study (1-day LRD arm = 143, 3-day LRD arm = 146). BBPS ≥2 was not significantly different in the two dietary regimens in any of the three colonic segments (71% vs. 72%, p = 0.9). The percentage of patients with incomplete preparation was similar in the two arms (9% vs. 9%; p = 1.0). No significant differences were found among colonoscopy findings in terms of abnormalities (81% vs. 84%, p = 0.8). Both groups scored similarly in overall tolerance to LRD (48% vs. 49%, p = 1.0) and also in whether they would have adopted a different dietary regimen (p = 0.3). Conclusion: Our multicenter randomized study confirmed that optimal bowel cleansing is reached through a 1-day LRD.
MeSH term(s)	Humans ; Cathartics ; Colonoscopy ; Colon ; Diet ; Preoperative Care ; Polyethylene Glycols
Chemical Substances	Cathartics ; Polyethylene Glycols (3WJQ0SDW1A)
Language	English
Publishing date	2023-01-17
Publishing country	Switzerland
Document type	Randomized Controlled Trial ; Multicenter Study ; Journal Article
ZDB-ID	632798-9
ISSN	1421-9875 ; 0257-2753
ISSN (online)	1421-9875
ISSN	0257-2753
DOI	10.1159/000529087
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Zs.A 1853: Show issues

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Article: Novel Hemostatic Gel As Rescue Therapy for Postsphincterotomy Bleeding Refractory to Self-Expanding Metallic Stent Placement.

Gagliardi, Mario / Oliviero, Giovanni / Fusco, Michele / Napoli, Marco / Sica, Andrea / Maurano, Attilio / Sica, Mariano / Zulli, Claudio

ACG case reports journal

2022 Volume 9, Issue 1, Page(s) e00744

Abstract: Endoscopic sphincterotomy (ES) is commonly performed during endoscopic retrograde cholangiopancreatography, and bleeding is a severe adverse event. PuraStat is a peptide developed as a hemostatic agent for endoscopy. We report its use as a hemostatic ... ...

Abstract	Endoscopic sphincterotomy (ES) is commonly performed during endoscopic retrograde cholangiopancreatography, and bleeding is a severe adverse event. PuraStat is a peptide developed as a hemostatic agent for endoscopy. We report its use as a hemostatic strategy in post-ES bleeding refractory to combined hemostasis. A patient with choledocholithiasis underwent endoscopic retrograde cholangiopancreatography for stone removal. After the ES, severe bleeding refractory to the injection of diluted epinephrine around the bleeding source and metal stent placement occurred. Hemostasis was achieved with the application of the hemostatic gel. We reported the use of novel self-assembling hemostatic gel as an effective therapeutic tool for post-ES refractory bleeding.
Language	English
Publishing date	2022-01-06
Publishing country	United States
Document type	Case Reports
ZDB-ID	2814825-3
ISSN	2326-3253
ISSN	2326-3253
DOI	10.14309/crj.0000000000000744
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Article ; Online: Fatal Outcome Consequent to an Endoscopic Full Thickness Resection of a Colonic Lateral Spreading Tumor: A Case Report.

Oliviero, Giovanni / Gagliardi, Mario / Napoli, Marco / Labianca, Orazio / D'Antonio, Antonio / Sica, Mariano / Maurano, Attilio / Zulli, Claudio

The American journal of case reports

2020 Volume 21, Page(s) e922855

Abstract: BACKGROUND Endoscopic full-thickness resection represents an innovative procedure, used in selected patients that allows lesions en-bloc resection with an integral wall specimen available for histopathological definition. Bleeding and perforation are ... ...

Abstract	BACKGROUND Endoscopic full-thickness resection represents an innovative procedure, used in selected patients that allows lesions en-bloc resection with an integral wall specimen available for histopathological definition. Bleeding and perforation are known to be the most frequent procedure-related adverse events. We report a case of entero-colonic fistula as complication of an endoscopic full-thickness resection. CASE REPORT A 77-year-old male, with a personal history of right-hemicolectomy for a colonic adenocarcinoma presented to our department for a routine colonoscopy that showed the presence of a 25 mm lateral spreading tumor localized at about 50 cm from the anal margin. A full-thickness resection of the lateral spreading tumor using the over-the-scope clip device was performed. After 4 weeks, because of abdominal pain, weight loss, diarrhea, and signs of malnutrition, the patient underwent a new colonoscopy showing hyperemic mucosa with ulcerations in all colonic segments and, at the site of the previous endoscopic full-thickness resection, an orifice of an entero-colonic fistula. The histological definition was suggestive for ulcerative proctocolitis and confirmed the presence of small bowel mucosa at fistula orifice. An intussusception at the level of fistula with consequent intestinal obstruction caused a worsening of clinical conditions and finally the patient death for a septic peritonitis. CONCLUSIONS Full thickness resection represents an innovative tool for en-bloc resection of gastrointestinal tumoral lesion, but procedural complications and limitations must be considered before performing this procedure.
MeSH term(s)	Aged ; Colon/surgery ; Colonic Neoplasms/surgery ; Colonoscopy ; Digestive System Fistula/etiology ; Endoscopic Mucosal Resection/adverse effects ; Fatal Outcome ; Humans ; Intestinal Fistula/etiology ; Intestinal Obstruction/etiology ; Male ; Peritonitis/etiology ; Proctocolitis/etiology ; Sepsis/etiology
Language	English
Publishing date	2020-07-30
Publishing country	United States
Document type	Case Reports ; Journal Article ; Video-Audio Media
ZDB-ID	2517183-5
ISSN	1941-5923 ; 1941-5923
ISSN (online)	1941-5923
ISSN	1941-5923
DOI	10.12659/AJCR.922855
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Article ; Online: Q-switched 1064 nm Nd-Yag nanosecond laser effects on skin barrier function and on molecular rejuvenation markers in keratinocyte-fibroblasts interaction.

De Filippis, Anna / Perfetto, Brunella / Guerrera, Luigi Pio / Oliviero, Giovanni / Baroni, Adone

Lasers in medical science

2018 Volume 34, Issue 3, Page(s) 595–605

Abstract: Skin represents an interface between internal and external environment; it protects human body by regulating the water loss and the maintenance of body temperature, defending against irritant and pathogen agents, and against physical, chemical, and UV ... ...

Abstract	Skin represents an interface between internal and external environment; it protects human body by regulating the water loss and the maintenance of body temperature, defending against irritant and pathogen agents, and against physical, chemical, and UV damage. It provides to essential physiological functions, such as the important antioxidant defense capacity; its protective/defensive function is performed by a high number of proteins, and shows important functions in maintenance of skin barrier homeostasis. Keratinocytes and fibroblasts play a pivotal role to determine or prevent skin aging in response to intrinsic or extrinsic stimuli, modulating cytokines and several biochemical factors. Non-ablative technologies are playing an increasing role in the management of skin aging, inducing a dermal remodeling without a visible epidermal damage. The objective of this study was to evaluate the effect of Q-switched 1064 Nd-YAG laser (Medlite Conbio C6 Nd-YAG laser, Cynosure USA) in skin barrier function, analyzing the constituents which are strongly altered in aging skin. Particularly, we evaluated the expression of filaggrin, TGase, HSP70, and aquaporins, on HaCaT cells. The expression of proinflammatory cytokines has been investigated too.As a second step of the study, we analyzed the modulation of the rejuvenation molecular markers on human skin fibroblasts (HDFs) stimulated with keratinocytes conditioned medium (KCM).Our results demonstrated that Q-switched 1064 nm Nd:YAG laser acts on the skin barrier function, increasing the expression of aquaporins, filaggrin, TGase, and HSP70, modulating the proinflammatory cytokines. In fibroblasts stimulated with keratinocytes conditioned medium (KCM) and irradiated with Q-switched 1064 nm Nd:YAG laser, we can observe a reduction of MMP-1 and an increase in procollagen, collagen type I, and elastin. Our results highlight that Q-switched 1064 nm Nd:YAG laser treatment could represent an effective weapon to fight skin aging.
MeSH term(s)	Biomarkers/metabolism ; Cell Communication/radiation effects ; Cell Line ; Cell Survival/radiation effects ; Cytokines/genetics ; Cytokines/metabolism ; Fibroblasts/cytology ; Fibroblasts/radiation effects ; Gene Expression Regulation/radiation effects ; Humans ; Inflammation Mediators/metabolism ; Keratinocytes/cytology ; Keratinocytes/radiation effects ; Lasers, Solid-State/therapeutic use ; Rejuvenation ; Skin/radiation effects ; Skin Aging/radiation effects ; Water
Chemical Substances	Biomarkers ; Cytokines ; Inflammation Mediators ; Water (059QF0KO0R)
Language	English
Publishing date	2018-10-01
Publishing country	England
Document type	Journal Article
ZDB-ID	632808-8
ISSN	1435-604X ; 0268-8921
ISSN (online)	1435-604X
ISSN	0268-8921
DOI	10.1007/s10103-018-2635-1
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Article ; Online: Replay to Response to Oliviero et al.'s Publication: "Impact of COVID-19 lockdown on symptoms in patients with functional gastrointestinal disorders: Relationship with anxiety and perceived stress".

Oliviero, Giovanni / Ruggiero, Luigi / D'Antonio, Elvira / Gagliardi, Mario / Nunziata, Rubino / Di Sarno, Alessandro / Abbatiello, Carmelina / Di Feo, Elvira / De Vivo, Simona / Santonicola, Antonella / Iovino, Paola

Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society

2021 Volume 33, Issue 11, Page(s) e14263

MeSH term(s)	Anxiety ; COVID-19 ; Communicable Disease Control ; Depression ; Gastrointestinal Diseases ; Humans ; SARS-CoV-2 ; Stress, Psychological
Language	English
Publishing date	2021-09-09
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	1186328-6
ISSN	1365-2982 ; 1350-1925
ISSN (online)	1365-2982
ISSN	1350-1925
DOI	10.1111/nmo.14263
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Article ; Online: Impact of COVID-19 lockdown on symptoms in patients with functional gastrointestinal disorders: Relationship with anxiety and perceived stress.

Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society

2021 Volume 33, Issue 5, Page(s) e14092

Abstract: Background: Psychological stress and anxiety, such those generated by forced quarantine, affect gastrointestinal symptoms course in patients with functional gastrointestinal disorders. Thus, our aim was to assess, in a cohort of patients regularly ... ...

Abstract	Background: Psychological stress and anxiety, such those generated by forced quarantine, affect gastrointestinal symptoms course in patients with functional gastrointestinal disorders. Thus, our aim was to assess, in a cohort of patients regularly followed up in a devoted outpatient clinic of Southern Italy, the association between their gastrointestinal symptoms changes, stress, and anxiety reported during the Italian lockdown. Methods: We recruited patients from the outpatient clinic of the University of Salerno, devoted to functional gastrointestinal disorders, selecting only patients for whom an evaluation was available in the last 6 months before the lockdown. Gastrointestinal symptoms were evaluated at each visit through standardized questionnaire and pooled in a database. On 45th days from the beginning of the lockdown, patients were re-assessed by phone with the same questionnaire. Anxiety and stress levels were assessed through a self-administered online questionnaire based on Generalized Anxiety Disorder 7 test and Perceived Stress Scale 10 test. Key results: The intensity-frequency scores of several upper gastrointestinal symptoms improved (Wilcoxon test <0.05). Higher anxiety levels had a higher risk of worsening chest pain (OR 1.3 [1.1-1.7]), waterbrash (OR 1.3 [1.0-1.7]), epigastric burning (OR 1.3 [1.0-1.6]), and abdominal pain (OR 1.6 [1.0-2.3]). When compared to the interval preceding the outbreak, half of the patients declared their symptoms remained unchanged, 13.6% worsened, and 36.4% improved. Conclusions and inferences: During the COVID-19 quarantine, there was an improvement of the majority of upper gastrointestinal symptoms in our patients, and anxiety seems an important risk of worsening few of them.
MeSH term(s)	Abdominal Pain/physiopathology ; Abdominal Pain/psychology ; Adult ; Anxiety/psychology ; COVID-19 ; Chest Pain/physiopathology ; Chest Pain/psychology ; Communicable Disease Control ; Dyspepsia/physiopathology ; Dyspepsia/psychology ; Female ; Gastrointestinal Diseases/physiopathology ; Gastrointestinal Diseases/psychology ; Heartburn/physiopathology ; Heartburn/psychology ; Humans ; Irritable Bowel Syndrome/physiopathology ; Irritable Bowel Syndrome/psychology ; Italy ; Male ; Middle Aged ; Patient Health Questionnaire ; Public Policy ; SARS-CoV-2 ; Stress, Psychological/psychology ; Surveys and Questionnaires
Language	English
Publishing date	2021-02-07
Publishing country	England
Document type	Journal Article
ZDB-ID	1186328-6
ISSN	1365-2982 ; 1350-1925
ISSN (online)	1365-2982
ISSN	1350-1925
DOI	10.1111/nmo.14092
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Article ; Online: Correction to: Effect of 1064-nm Q-switched Nd:YAG laser on invasiveness and innate immune response in keratinocytes infected with Candida albicans.

Baroni, Adone / De Filippis, Anna / Oliviero, Giovanni / Fusco, Alessandra / Perfetto, Brunella / Buommino, Elisabetta / Donnarumma, Giovanna

Lasers in medical science

2018 Volume 33, Issue 3, Page(s) 691

Abstract: In the published online version of the article, the authors' given and family names were incorrectly captured. The corrected names are shown in the author group section above. ...

Abstract	In the published online version of the article, the authors' given and family names were incorrectly captured. The corrected names are shown in the author group section above.
Language	English
Publishing date	2018
Publishing country	England
Document type	Journal Article ; Published Erratum
ZDB-ID	632808-8
ISSN	1435-604X ; 0268-8921
ISSN (online)	1435-604X
ISSN	0268-8921
DOI	10.1007/s10103-018-2448-2
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Zs.A 2148: Show issues

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Article ; Online: Effect of 1064-nm Q-switched Nd:YAG laser on invasiveness and innate immune response in keratinocytes infected with Candida albicans.

Baroni, Adone / De Filippis, Anna / Oliviero, Giovanni / Fusco, Alessandra / Perfetto, Brunella / Buommino, Elisabetta / Donnarumma, Giovanna

Lasers in medical science

2018 Volume 33, Issue 5, Page(s) 941–948

Abstract: Candida albicans is an opportunistic pathogen commensal in the oral cavity, vagina, and healthy skin. Common therapeutic options for fungal infections are topical or systemic antifungal drugs. Recently, in cutaneous pathologies, lasers and light-based ... ...

Abstract	Candida albicans is an opportunistic pathogen commensal in the oral cavity, vagina, and healthy skin. Common therapeutic options for fungal infections are topical or systemic antifungal drugs. Recently, in cutaneous pathologies, lasers and light-based treatments have emerged showing few contraindications and minimal side effects. The Q-switched (Nd-YAG) laser at a wavelength of 1064 nm has been shown to be useful in dermatology, dentistry, and some other medical specialties. It is used to treat onychomycoses, warts, and wounds and in some other treatments. We analyzed the effect of Q-switched (Nd-YAG) laser 1064 nm on human keratinocytes infected with C. albicans. In particular, we evaluated the effect of laser on invasiveness of C. albicans and on inflammatory and protective response of HaCaT cells infected. The results obtained did not show inhibitory, fungicidal, or fungistatic effects of laser on yeast; in addition, laser did not affect HaCaT vitality. HaCaT cells infected with C. albicans and irradiated with laser showed a reduction of invasiveness of TNF-α and IL8 gene expression and an increase of immunomodulatory cytokines such as TGFβ. Furthermore, laser induces a significant over-expression of HSP70B (heat shock protein) and of HBD-2 (Human β defensin-2) in HaCaT infected with C. albicans, compared to the untreated control. The use of Q-switched Nd:YAG laser in skin mycosis caused by C. albicans reduces yeast invasiveness in keratinocytes, downregulates inflammatory activities, and facilitates cytoprotection and antimicrobial defense. Our results offer a promising therapeutic strategy in the management of skin candidiasis, also in combination with conventional therapies.
MeSH term(s)	Candida albicans/radiation effects ; Cell Line ; Cell Survival/radiation effects ; Female ; HSP70 Heat-Shock Proteins/genetics ; HSP70 Heat-Shock Proteins/metabolism ; Humans ; Immunity, Innate/radiation effects ; Inflammation/pathology ; Keratinocytes/immunology ; Keratinocytes/microbiology ; Keratinocytes/pathology ; Keratinocytes/radiation effects ; Lasers, Solid-State/therapeutic use ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Up-Regulation/radiation effects ; beta-Defensins/genetics ; beta-Defensins/metabolism
Chemical Substances	HSP70 Heat-Shock Proteins ; RNA, Messenger ; beta-Defensins
Language	English
Publishing date	2018-07
Publishing country	England
Document type	Journal Article
ZDB-ID	632808-8
ISSN	1435-604X ; 0268-8921
ISSN (online)	1435-604X
ISSN	0268-8921
DOI	10.1007/s10103-017-2407-3
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