LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 50

Search options

  1. Article ; Online: Immune cell profiling in the joint following human and murine articular fracture.

    Furman, B D / Zeitlin, J H / Buchanan, M W / Huebner, J L / Kraus, V B / Yi, J S / Adams, S B / Olson, S A

    Osteoarthritis and cartilage

    2021  Volume 29, Issue 6, Page(s) 915–923

    Abstract: Objective: Human and in vivo animal research implicates inflammation following articular fracture as contributing to post-traumatic arthritis. However, relevant immune cell subsets present following injury are currently undefined. Immunophenotyping ... ...

    Abstract Objective: Human and in vivo animal research implicates inflammation following articular fracture as contributing to post-traumatic arthritis. However, relevant immune cell subsets present following injury are currently undefined. Immunophenotyping human and murine synovial fluid may help to identify immune cell populations that play key roles in the response to articular fracture.
    Methods: Immunophenotyping by polychromatic flow cytometry was performed on human and mouse synovial fluid following articular fracture. Specimens were collected in patients with closed ankle fracture at the time of surgical fixation and from C57BL/6 mice with closed articular knee fracture. Immune cells were collected from injured and uninjured joints in mice via a novel cell isolation method. Whole blood samples were also collected. Immunohistochemistry (IHC) was performed on mouse synovial tissue to assess for macrophages and T cells.
    Results: Following intra-articular fracture, the prominent human synovial fluid immune cell subset was CD3+ T cells, containing both CD4+ and CD8+ T cells. In mice, infiltration of CD45+ immune cells in synovial fluid of the fractured limb was dominated by CD19+ B cells and CD3+ T cells at 7 days after intra-articular fracture. We also detected adaptive immune cells, including macrophages, NK cells, dendritic cells and monocytes. Macrophage and T cell findings were supported by IHC of murine synovial tissue.
    Conclusions: Determining specific cell populations that mediate the immune response is essential to elucidating the chain of events initiated after injury and may be an important step in identifying potential immune signatures predictive of PTA susceptibility or potential therapeutic targets.
    MeSH term(s) Animals ; Female ; Fractures, Bone/immunology ; Humans ; Immune System/cytology ; Immunophenotyping ; Joints/injuries ; Male ; Mice ; Mice, Inbred C57BL ; Synovial Fluid/cytology
    Language English
    Publishing date 2021-02-25
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1167809-4
    ISSN 1522-9653 ; 1063-4584
    ISSN (online) 1522-9653
    ISSN 1063-4584
    DOI 10.1016/j.joca.2021.02.565
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3828: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Intra-articular depletion of macrophages increases acute synovitis and alters macrophage polarity in the injured mouse knee.

    Bailey, K N / Furman, B D / Zeitlin, J / Kimmerling, K A / Wu, C-L / Guilak, F / Olson, S A

    Osteoarthritis and cartilage

    2020  Volume 28, Issue 5, Page(s) 626–638

    Abstract: Objective: Acute synovial inflammation following joint trauma is associated with posttraumatic arthritis. Synovial macrophages have been implicated in degenerative changes. In this study, we sought to elucidate the role of intra-articular macrophages in ...

    Abstract Objective: Acute synovial inflammation following joint trauma is associated with posttraumatic arthritis. Synovial macrophages have been implicated in degenerative changes. In this study, we sought to elucidate the role of intra-articular macrophages in the acute inflammatory response to fracture in the mouse knee.
    Method: A closed articular fracture was induced in two models of synovial macrophage depletion: genetically-modified MaFIA mice administered AP20187 to induce programmed macrophage apoptosis, and wild-type C57BL/6 mice administered clodronate liposomes, both via intra-articular injection. Synovial inflammation, bone morphology, and levels of F4/80+ macrophages, NOS2+ M1 macrophages, and CD206+ M2 macrophages were quantified 7 days after fracture using histology and micro-computed tomography.
    Results: Intra-articular macrophage depletion with joint injury did not reduce acute synovitis or the number of synovial macrophages 7 days after fracture in either macrophage-depleted MaFIA mice or in clodronate-treated C57BL/6 mice. In macrophage-depleted MaFIA mice, macrophage polarity shifted to a dominance of M1 macrophages and a reduction of M2 macrophages in the synovial stroma, indicating a shift in M1/M2 macrophage ratio in the joint following injury. Interestingly, MaFIA mice depleted 2 days prior to fracture demonstrated increased synovitis (P = 0.003), reduced bone mineral density (P = 0.0004), higher levels of M1 macrophages (P = 0.013), and lower levels of M2 macrophages (not statistically significant, P=0.084) compared to control-treated MaFIA mice.
    Conclusion: Our findings indicate that macrophages play a critical immunomodulatory role in the acute inflammatory response surrounding joint injury and suggest that inhibition of macrophage function can have prominent effects on joint inflammation and bone homeostasis after joint trauma.
    MeSH term(s) Animals ; Apoptosis ; Calcium-Binding Proteins/metabolism ; Clodronic Acid ; Genes, Transgenic, Suicide ; Injections, Intra-Articular ; Intra-Articular Fractures/diagnostic imaging ; Intra-Articular Fractures/immunology ; Intra-Articular Fractures/pathology ; Knee Injuries/diagnostic imaging ; Knee Injuries/immunology ; Knee Injuries/pathology ; Lectins, C-Type/metabolism ; Liposomes ; Macrophages/immunology ; Macrophages/metabolism ; Male ; Mannose Receptor ; Mannose-Binding Lectins/metabolism ; Mice ; Mice, Transgenic ; Nitric Oxide Synthase Type II/metabolism ; Osteoarthritis, Knee/immunology ; Receptors, Cell Surface/metabolism ; Receptors, G-Protein-Coupled/metabolism ; Synovitis/diagnostic imaging ; Synovitis/immunology ; Synovitis/pathology ; Tacrolimus/analogs & derivatives ; X-Ray Microtomography
    Chemical Substances AP20187 ; Adgre1 protein, mouse ; Calcium-Binding Proteins ; Lectins, C-Type ; Liposomes ; Mannose Receptor ; Mannose-Binding Lectins ; Receptors, Cell Surface ; Receptors, G-Protein-Coupled ; Clodronic Acid (0813BZ6866) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Nos2 protein, mouse (EC 1.14.13.39) ; Tacrolimus (WM0HAQ4WNM)
    Language English
    Publishing date 2020-02-08
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1167809-4
    ISSN 1522-9653 ; 1063-4584
    ISSN (online) 1522-9653
    ISSN 1063-4584
    DOI 10.1016/j.joca.2020.01.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3828: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: Early recovery after hip arthroscopy for femoroacetabular impingement syndrome: a prospective, observational study.

    Cunningham, D J / Lewis, B D / Hutyra, C A / Mather, R C / Olson, S A

    Journal of hip preservation surgery

    2017  Volume 4, Issue 4, Page(s) 299–307

    Abstract: The early post-operative course after hip arthroscopy for femoroacetabular impingement syndrome has not been thoroughly characterized or correlated to factors that may influence recovery. The aim of this study was to report on early pain, function and ... ...

    Abstract The early post-operative course after hip arthroscopy for femoroacetabular impingement syndrome has not been thoroughly characterized or correlated to factors that may influence recovery. The aim of this study was to report on early pain, function and attitudes towards rehabilitation and to determine predictors of early recovery after hip arthroscopy. Sixty-two patients reported pre-operative pain, iHOT-12 (hip functional score), psychological status and other baseline characteristics. Pain, iHOT-12, hip flexion and several other outcomes were measured through 6 weeks post-operative. Baseline characteristics were correlated with outcomes using univariate and multivariable models. Pain relief started on post-operative day 1 and consistently improved throughout the 6 weeks of follow-up. The average patient's pain was reduced from a pre-operative level of 5/10 to 2/10 by 6 weeks post-operative. Similarly, iHOT-12 improved from 33/100 to 57/100 whereas hip flexion increased by 9° by 6 weeks post-operative. At 2 weeks post-operative, pre-operative anti-inflammatory usage was associated with greater improvement in pain and swelling; pre-operative opioid usage with poorer patient-reported helpfulness of and adherence to rehabilitation; and higher ASA (American Society of Anesthesiologists) score and lower procedure time with improvement of the pre-operative pain complaint. At 6 weeks, greater depression was associated with lower post-operative pain reduction but greater pre-operative pain complaint improvement. Continuous passive motion usage was associated with increased hip flexion. Pain improved from pre-operative by Day 1 after hip arthroscopy, and early functional improvements were seen by 6 weeks post-operative. Pre-operative anti-inflammatory and opioid usage, depression, race, ASA score, procedure time and continuous passive motion usage were significantly associated with study outcomes.
    Language English
    Publishing date 2017-07-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 2773022-0
    ISSN 2054-8397
    ISSN 2054-8397
    DOI 10.1093/jhps/hnx026
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Open fractures of the tibial shaft.

    Olson, S A

    Instructional course lectures

    1997  Volume 46, Page(s) 293–302

    MeSH term(s) Antibiotic Prophylaxis/methods ; Bone Transplantation ; Debridement ; Emergencies ; Fractures, Open/diagnosis ; Fractures, Open/therapy ; Humans ; Physical Therapy Modalities ; Suture Techniques ; Therapeutic Irrigation/methods ; Tibial Fractures/diagnosis ; Tibial Fractures/therapy
    Language English
    Publishing date 1997
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 802490-x
    ISSN 0065-6895
    ISSN 0065-6895
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1977: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 230: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: MacVector: sequence comparisons using a matrix method.

    Olson, S A

    Methods in molecular biology (Clifton, N.J.)

    1994  Volume 25, Page(s) 215–225

    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Computer Graphics ; DNA/genetics ; Databases, Factual ; Microcomputers ; Molecular Sequence Data ; Proteins/genetics ; Sequence Alignment/methods ; Sequence Alignment/statistics & numerical data ; Software
    Chemical Substances Proteins ; DNA (9007-49-2)
    Language English
    Publishing date 1994
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1385/0-89603-276-0:215
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: MacVector: restriction enzyme analysis.

    Olson, S A

    Methods in molecular biology (Clifton, N.J.)

    1994  Volume 25, Page(s) 227–236

    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Binding Sites/genetics ; Computer Graphics ; DNA/genetics ; DNA Restriction Enzymes ; Databases, Factual ; Microcomputers ; Molecular Sequence Data ; Proteins/genetics ; Restriction Mapping ; Sequence Analysis, DNA/methods ; Sequence Analysis, DNA/statistics & numerical data ; Software
    Chemical Substances Proteins ; DNA (9007-49-2) ; DNA Restriction Enzymes (EC 3.1.21.-)
    Language English
    Publishing date 1994
    Publishing country United States
    Document type Journal Article
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1385/0-89603-276-0:227
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article: MacVector: aligning sequences.

    Olson, S A

    Methods in molecular biology (Clifton, N.J.)

    1994  Volume 25, Page(s) 203–214

    MeSH term(s) Algorithms ; Amino Acid Sequence ; Base Sequence ; Computer Graphics ; DNA/genetics ; Databases, Factual ; Microcomputers ; Molecular Sequence Data ; Proteins/genetics ; Sequence Alignment/methods ; Sequence Alignment/statistics & numerical data ; Software ; Tubulin/genetics
    Chemical Substances Proteins ; Tubulin ; DNA (9007-49-2)
    Language English
    Publishing date 1994
    Publishing country United States
    Document type Journal Article
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1385/0-89603-276-0:203
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article: MacVector: an integrated sequence analysis program for the Macintosh.

    Olson, S A

    Methods in molecular biology (Clifton, N.J.)

    1994  Volume 25, Page(s) 195–201

    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Databases, Factual ; Microcomputers ; Nucleic Acids/genetics ; Proteins/genetics ; Sequence Analysis/methods ; Sequence Analysis/statistics & numerical data ; Software
    Chemical Substances Nucleic Acids ; Proteins
    Language English
    Publishing date 1994
    Publishing country United States
    Document type Journal Article
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1385/0-89603-276-0:195
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article: MacVector: protein analysis.

    Olson, S A

    Methods in molecular biology (Clifton, N.J.)

    1994  Volume 25, Page(s) 237–246

    MeSH term(s) Amino Acid Sequence ; Computer Graphics ; Cytochrome c Group/chemistry ; Cytochrome c Group/genetics ; Humans ; Isoelectric Point ; Microcomputers ; Molecular Sequence Data ; Molecular Structure ; Protein Structure, Secondary ; Proteins/chemistry ; Proteins/genetics ; Software
    Chemical Substances Cytochrome c Group ; Proteins
    Language English
    Publishing date 1994
    Publishing country United States
    Document type Journal Article
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1385/0-89603-276-0:237
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article: Medium-term results of the Bernese periacetabular osteotomy in the treatment of symptomatic developmental dysplasia of the hip.

    Garras, D N / Crowder, T T / Olson, S A

    The Journal of bone and joint surgery. British volume

    2007  Volume 89, Issue 6, Page(s) 721–724

    Abstract: We studied the medium-term outcome of the Bernese periacetabular osteotomy in 52 patients (58 hips) with symptomatic developmental dysplasia of the hip and a mean age of 37.6 years (13 to 48). The operations were performed between 1993 and 2005 by the ... ...

    Abstract We studied the medium-term outcome of the Bernese periacetabular osteotomy in 52 patients (58 hips) with symptomatic developmental dysplasia of the hip and a mean age of 37.6 years (13 to 48). The operations were performed between 1993 and 2005 by the senior author with a mean follow-up of 66.7 months (13 to 153). There were 42 women (47 hips) and ten men (11 hips). Of these patients, 24 (30 hips) had an osteotomy on the right side and 22 (28 hips) on the left. Six patients had bilateral operations. The clinical outcome was assessed using the modified Merle d'Aubigne scale, and pre- and post-operative radiological evaluation using the modified Tonnis osteoarthritis score, the centre-edge angle, the acetabular index, the status of Shenton's line, and the cross-over sign. The mean centre-edge angle and the acetabular index were 14 degrees (2 degrees to 34 degrees ) and 23.6 degrees (0 degrees to 40 degrees ) before operation, and 36.6 degrees (16 degrees to 72 degrees ) and 7.9 degrees (0 degrees to 28 degrees ) after, respectively (p < 0.001, analysis of variance (ANOVA)). Shenton's line was intact in 23 hips (39.6%) before operation and in 48 hips (82.8%) after. The cross-over sign was present in 31 hips (53.4%) before and in three hips (5.2%) after operation (p < 0.001, ANOVA). The total Merle d'Aubigne clinical score improved from a mean of 12.6 (9 to 15) to 16.0 (12 to 18) points (p < 0.001, ANOVA). Only four hips required subsequent total hip replacement. Our results indicate that the Bernese periacetabular osteotomy provides good symptomatic relief for patients with little to no arthritis (Tonnis type 0 or 1) with an underlying deformity that can be corrected to a position of a stable, congruent hip joint.
    MeSH term(s) Adolescent ; Adult ; Female ; Follow-Up Studies ; Hip Dislocation, Congenital/diagnostic imaging ; Hip Dislocation, Congenital/surgery ; Humans ; Male ; Middle Aged ; Osteoarthritis, Hip/diagnostic imaging ; Osteoarthritis, Hip/etiology ; Osteoarthritis, Hip/prevention & control ; Osteotomy/methods ; Radiography ; Treatment Outcome
    Language English
    Publishing date 2007-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 220626-2
    ISSN 2044-5377 ; 0301-620X ; 0447-9076
    ISSN (online) 2044-5377
    ISSN 0301-620X ; 0447-9076
    DOI 10.1302/0301-620X.89B6.18805
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uk I Zs.58/1: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top