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  1. Article ; Online: Re-emergence of dengue, chikungunya, and Zika viruses in 2021 after a 10-year gap in Gabon.

    Ushijima, Yuri / Abe, Haruka / Mbadinga, Marien J V M / Ondo, Georgelin Nguema / Bikangui, Rodrigue / Agnandji, Selidji T / Lell, Bertrand / Yasuda, Jiro

    IJID Regions (Online)

    2022  Volume 5, Page(s) 68–71

    Abstract: Mosquito-borne viral infections are a major concern in endemic areas, such as Africa. Although outbreaks have been reported throughout Africa, only a few surveillance studies have been conducted in Gabon since the outbreaks of dengue virus (DENV) and ... ...

    Abstract Mosquito-borne viral infections are a major concern in endemic areas, such as Africa. Although outbreaks have been reported throughout Africa, only a few surveillance studies have been conducted in Gabon since the outbreaks of dengue virus (DENV) and chikungunya virus (CHIKV) in 2010. Therefore, the current situation is unknown. This study aimed to investigate the presence of arboviruses, especially DENV (serotypes 1-4), CHIKV, and Zika virus (ZIKV), in Gabon, Central Africa. Between 2020 and 2021, we collected 1060 serum samples from febrile patients and screened them against viruses using reverse transcription-quantitative PCR. We detected two DENV serotypes 1 (DENV-1), one CHIKV, and one ZIKV, and subsequently analyzed the genome sequences. To determine the genetic diversity and transmission route of the viruses, phylogenetic analysis was performed using complete or partial genome sequences. The DENV-1 and CHIKV strains detected in this study were closely related to the previous Gabonese strains, whereas the recent ZIKV strain was genetically different from a strain detected in 2007 in Gabon. This study provides new genomic information on DENV-1, CHIKV, and ZIKV that were detected in Gabon and insight into the circulation of the viruses in the country and their introduction from neighboring African countries.
    Language English
    Publishing date 2022-09-05
    Publishing country England
    Document type Journal Article
    ISSN 2772-7076
    ISSN (online) 2772-7076
    DOI 10.1016/j.ijregi.2022.08.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Delays in the arrival of the waves of COVID-19: a comparison between Gabon and the African continent.

    Abe, Haruka / Ushijima, Yuri / Bikangui, Rodrigue / Ondo, Georgelin Nguema / Lell, Bertrand / Adegnika, Ayola A / Yasuda, Jiro

    The Lancet. Microbe

    2022  Volume 3, Issue 7, Page(s) e476

    MeSH term(s) Blacks ; COVID-19/epidemiology ; Gabon/epidemiology ; Humans
    Language English
    Publishing date 2022-04-21
    Publishing country England
    Document type Letter ; Research Support, Non-U.S. Gov't
    ISSN 2666-5247
    ISSN (online) 2666-5247
    DOI 10.1016/S2666-5247(22)00091-X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Genetic Diversity of Hepatitis B and C Viruses Revealed by Continuous Surveillance from 2015 to 2021 in Gabon, Central Africa.

    Abe, Haruka / Ushijima, Yuri / Bikangui, Rodrigue / Ondo, Georgelin Nguema / Pemba, Christelle M / Zadeh, Vahid R / Mpingabo, Patrick I / Ueda, Hayato / Agnandji, Selidji T / Lell, Bertrand / Yasuda, Jiro

    Microorganisms

    2023  Volume 11, Issue 8

    Abstract: Viral hepatitis remains one of the largest public health concerns worldwide. Especially in Central Africa, information on hepatitis virus infections has been limited, although the prevalence in this region has been reported to be higher than the global ... ...

    Abstract Viral hepatitis remains one of the largest public health concerns worldwide. Especially in Central Africa, information on hepatitis virus infections has been limited, although the prevalence in this region has been reported to be higher than the global average. To reveal the current status of hepatitis B and C virus (HBV and HCV) infections and the genetic diversity of the viruses, we conducted longitudinal surveillance in Gabon. We detected 22 HBV and 9 HCV infections in 2047 patients with febrile illness. Genetic analyses of HBV identified subgenotype A1 for the first time in Gabon and an insertion generating a frameshift to create an X-preC/C fusion protein. We also revealed that most of the detected HCVs belonged to the "Gabon-specific" HCV subtype 4e (HCV-4e), and the entire nucleotide sequence of the HCV-4e polyprotein was determined to establish the first reference sequence. The HCV-4e strains possessed resistance-associated substitutions similar to those of other HCV-4 strains, indicating that the use of direct-acting antiviral therapy may be complex. These results provide a better understanding of the current situation of hepatitis B and C virus infections in Central Africa and will help public health organizations develop effective countermeasures to eliminate chronic viral hepatitis in this region.
    Language English
    Publishing date 2023-08-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11082046
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Long-term validation of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of SARS-CoV-2 from March 2020 to October 2021 in Central Africa, Gabon.

    Abe, Haruka / Ushijima, Yuri / Bikangui, Rodrigue / Ondo, Georgelin Nguema / Moure, Ayong / Yali-Assy-Oyamli, Yoric / Yoshikawa, Rokusuke / Lell, Bertrand / Adegnika, Ayola A / Yasuda, Jiro

    PLoS neglected tropical diseases

    2022  Volume 16, Issue 12, Page(s) e0010964

    Abstract: Background: Despite the development of several methods for diagnosing COVID-19, long-term validation of such methods remains limited. In the early phase of the COVID-19 pandemic, we developed a rapid and sensitive diagnostic method based on reverse ... ...

    Abstract Background: Despite the development of several methods for diagnosing COVID-19, long-term validation of such methods remains limited. In the early phase of the COVID-19 pandemic, we developed a rapid and sensitive diagnostic method based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) methodology, which is suitable for point-of-care application or for use in resource-limited settings to detect SARS-CoV-2. To assess the applicability of the RT-LAMP assay technique to resource-limited regions, such as rural areas in Africa, and to verify the usability of the method against various SARS-CoV-2 variants, the method was validated using clinical samples collected longitudinally during the pandemic.
    Methodology/principal findings: First, the sensitivity of the RT-LAMP assay for detecting 10 SARS-CoV-2 variants was evaluated using viral RNA samples extracted from cell culture with a portable battery-supported device, resulting in the successful detection of 20-50 copies of the viral genome within 15 min, regardless of the variant. COVID-19 positive samples collected in Gabon between March 2020 and October 2021 were used to evaluate the sensitivity of the assay and to calculate the copy number of the SARS-CoV-2 genome. More than 292 copies of the viral genome were detected with 100% probability within 15 min in almost all tests.
    Conclusions: This long-term validation study clearly demonstrated the applicability of the RT-LAMP assay for the clinical diagnosis of COVID-19 in resource-limited settings of Africa, such as rural areas in Gabon. The results show the potential of the assay as a promising COVID-19 diagnostic method, especially in rural and remote regions located far from the official diagnosis facilities in urban or semi-urban areas.
    Language English
    Publishing date 2022-12-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2429704-5
    ISSN 1935-2735 ; 1935-2735
    ISSN (online) 1935-2735
    ISSN 1935-2735
    DOI 10.1371/journal.pntd.0010964
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: SARS-CoV-2 emerging variants in Africa: view from Gabon.

    Zoa-Assoumou, Samira / Ndeboko, Bénédicte / Manouana, Gédéon Prince / Houechenou, Rotimi Myrabelle Avome / Bikangui, Rodrigue / Mveang-Nzoghe, Amandine / Ondo, Georgelin Nguema / Mbongo-Kama, Elvyre / Lell, Bertrand / Abe, Haruka / Ushijima, Yuri / Antchouey, Anne-Marie / Yasuda, Jiro / Adegnika, Ayola Akim / Siawaya, Joel Fleury Djoba

    The Lancet. Microbe

    2021  Volume 2, Issue 8, Page(s) e349

    MeSH term(s) Antibodies, Viral ; COVID-19/epidemiology ; Gabon/epidemiology ; Humans ; SARS-CoV-2/genetics
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2021-06-08
    Publishing country England
    Document type Letter ; Research Support, Non-U.S. Gov't
    ISSN 2666-5247
    ISSN (online) 2666-5247
    DOI 10.1016/S2666-5247(21)00125-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Unrecognized introduction of SARS-CoV-2 variants of concern to Central Africa: Import and local transmission of B.1.1.7 in Gabon in the very early stage of the variant spread to the African continent.

    Abe, Haruka / Ushijima, Yuri / Bikangui, Rodrigue / Zoa-Assoumou, Samira / Ondo, Georgelin Nguema / Manouana, Gédéon Prince / More, Ayong / Skarwan, Emilio / Yali-Assy-Oyamli, Yoric / Ndeboko, Bénédicte / Myrabelle Avome Houechenou, Rotimi / Djoba Siawaya, Joel Fleury / Lell, Bertrand / Adegnika, Ayola Akim / Yasuda, Jiro

    Journal of medical virology

    2021  Volume 93, Issue 10, Page(s) 6054–6058

    Abstract: The rapid spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant of concern with higher infectivity has already resulted in the enormous increase in infection cases worldwide. We report an unrecognized introduction of the ... ...

    Abstract The rapid spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant of concern with higher infectivity has already resulted in the enormous increase in infection cases worldwide. We report an unrecognized introduction of the variant B.1.1.7 in Gabon in December 2020, which was the initial phase of the variant introduction to Africa. The B.1.1.7 variant was also detected in a hospitalized patient in January 2021, indicating a rapid spread of the variant in Gabon since its first detection. Phylogenetic analysis revealed that the detected B.1.1.7 variants originated from the distinct regions, strongly suggesting that the B.1.1.7 variant had been repeatedly introduced to Gabon since December 2020. These results provide insights on the unrecognized risks of infections with variants of concern, and show the necessity to conduct continuous genomic monitoring for immediate alert and control of novel SARS-CoV-2 variant infections.
    MeSH term(s) Africa, Central/epidemiology ; COVID-19/epidemiology ; COVID-19/transmission ; COVID-19/virology ; Genome, Viral ; Humans ; Mutation ; Phylogeny ; RNA, Viral ; SARS-CoV-2/genetics ; Whole Genome Sequencing
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2021-07-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.27164
    Database MEDical Literature Analysis and Retrieval System OnLINE

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