LIVIVO - Search results -

Search results

Result 1 - 10 of total 29

Search options

Article ; Online: Editorial: The role of red blood cells in the immune response of fish.

Ortega-Villaizan, Maria Del Mar / Coll, Julio / Rimstad, Espen

2022 Volume 13, Page(s) 1005546

MeSH term(s)	Animals ; Erythrocyte Count ; Erythrocytes/physiology ; Fishes ; Immunity
Language	English
Publishing date	2022-09-06
Publishing country	Switzerland
Document type	Editorial ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.1005546
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Fish Innate Immune Response to Viral Infection-An Overview of Five Major Antiviral Genes.

Ortega-Villaizan, Maria Del Mar / Chico, Veronica / Perez, Luis

Viruses

2022 Volume 14, Issue 7

Abstract: Fish viral diseases represent a constant threat to aquaculture production. Thus, a better understanding of the cellular mechanisms involved in establishing an antiviral state associated with protection against virus replication and pathogenesis is ... ...

Abstract	Fish viral diseases represent a constant threat to aquaculture production. Thus, a better understanding of the cellular mechanisms involved in establishing an antiviral state associated with protection against virus replication and pathogenesis is paramount for a sustainable aquaculture industry. This review summarizes the current state of knowledge on five selected host innate immune-related genes in response to the most relevant viral pathogens in fish farming. Viruses have been classified as ssRNA, dsRNA, and dsDNA according to their genomes, in order to shed light on what those viruses may share in common and what response may be virus-specific, both in vitro (cell culture) as well as in vivo. Special emphasis has been put on trying to identify markers of resistance to viral pathogenesis. That is, those genes more often associated with protection against viral disease, a key issue bearing in mind potential applications into the aquaculture industry.
MeSH term(s)	Animals ; Antiviral Agents ; Fish Diseases ; Fishes ; Immunity, Innate ; Virus Diseases/genetics ; Virus Diseases/veterinary ; Viruses/genetics
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2022-07-15
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v14071546
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Fish Innate Immune Response to Viral Infection—An Overview of Five Major Antiviral Genes

Ortega-Villaizan, Maria del Mar / Chico, Veronica / Perez, Luis

Viruses. 2022 July 15, v. 14, no. 7

2022

Abstract	Fish viral diseases represent a constant threat to aquaculture production. Thus, a better understanding of the cellular mechanisms involved in establishing an antiviral state associated with protection against virus replication and pathogenesis is paramount for a sustainable aquaculture industry. This review summarizes the current state of knowledge on five selected host innate immune-related genes in response to the most relevant viral pathogens in fish farming. Viruses have been classified as ssRNA, dsRNA, and dsDNA according to their genomes, in order to shed light on what those viruses may share in common and what response may be virus-specific, both in vitro (cell culture) as well as in vivo. Special emphasis has been put on trying to identify markers of resistance to viral pathogenesis. That is, those genes more often associated with protection against viral disease, a key issue bearing in mind potential applications into the aquaculture industry.
Keywords	DNA ; aquaculture industry ; cell culture ; fish ; innate immunity ; pathogenesis ; virus replication
Language	English
Dates of publication	2022-0715
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v14071546
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Nucleated red blood cells: Immune cell mediators of the antiviral response.

Nombela, Ivan / Ortega-Villaizan, Maria Del Mar

PLoS pathogens

2018 Volume 14, Issue 4, Page(s) e1006910

MeSH term(s)	Antiviral Agents/therapeutic use ; Cell Nucleus/immunology ; Erythrocytes/immunology ; Humans ; Virus Diseases/immunology ; Virus Diseases/prevention & control ; Viruses/drug effects ; Viruses/immunology
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2018-04-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1006910
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Induction of foxp3 during the Crosstalk between Antigen Presenting Like-Cells MHCII+CD83+ and Splenocytes CD4+IgM− in Rainbow Trout

Morales-Lange, Byron / Nombela, Ivan / Ortega-Villaizán, María Del Mar / Imarai, Mónica / Schmitt, Paulina / Mercado, Luis

Biology. 2021 Apr. 13, v. 10, no. 4

2021

Abstract: In fish, the spleen is one of the major immune organs in the animal, and the splenocytes could play a key role in the activation and modulation of the immune response, both innate and adaptive. However, the crosstalk between different types of immune ... ...

Abstract	In fish, the spleen is one of the major immune organs in the animal, and the splenocytes could play a key role in the activation and modulation of the immune response, both innate and adaptive. However, the crosstalk between different types of immune cells in the spleen has been poorly understood. In this work, an in vitro strategy is carried out to obtain and characterize mononuclear splenocytes from rainbow trout, using biomarkers associated with lymphocytes (CD4 and IgM) and antigen-presenting cells (CD83 and MHC II). Using these splenocytes, co-cultures of 24 and 48 h are used to determine the gene expression of master transcriptional factors that coordinate the polarization of T cells (t-bet, gata3, and foxp3). The results show a proportional upregulation of foxp3 (compared to t-bet and gata3) in co-cultures (at 24 h) of IFNγ-induced splenocytes with and without stimulation of Piscirickettsia salmonis proteins. In addition, foxp3 upregulation was established in co-cultures with IFNγ-induced cells and in cells only stimulated previously with P. salmonis proteins at 48 h of co-culture. These results show a potential communication between antigen-presenting-like cells and lymphocyte in the spleen, which could be induced towards a Treg phenotype.
Keywords	GATA transcription factors ; Oncorhynchus mykiss ; Piscirickettsia salmonis ; antigens ; biomarkers ; coculture ; fish ; gene expression ; immune response ; phenotype ; spleen ; splenocytes ; transcription (genetics)
Language	English
Dates of publication	2021-0413
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology10040324
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Induction of

Morales-Lange, Byron / Nombela, Ivan / Ortega-Villaizán, María Del Mar / Imarai, Mónica / Schmitt, Paulina / Mercado, Luis

Biology

2021 Volume 10, Issue 4

Abstract	In fish, the spleen is one of the major immune organs in the animal, and the splenocytes could play a key role in the activation and modulation of the immune response, both innate and adaptive. However, the crosstalk between different types of immune cells in the spleen has been poorly understood. In this work, an in vitro strategy is carried out to obtain and characterize mononuclear splenocytes from rainbow trout, using biomarkers associated with lymphocytes (CD4 and IgM) and antigen-presenting cells (CD83 and MHC II). Using these splenocytes, co-cultures of 24 and 48 h are used to determine the gene expression of master transcriptional factors that coordinate the polarization of T cells (
Language	English
Publishing date	2021-04-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology10040324
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Immunomodulatory Lectin-like Peptides for Fish Erythrocytes-Targeting as Potential Antiviral Drug Delivery Platforms.

Salvador-Mira, Maria / Chico, Veronica / Arostica, Monica / Guzmán, Fanny / Roher, Nerea / Perez, Luis / Ortega-Villaizan, Maria Del Mar

International journal of molecular sciences

2021 Volume 22, Issue 21

Abstract: One of the challenges of science in disease prevention is optimizing drug and vaccine delivery. Until now, many strategies have been employed in this sector, but most are quite complex and labile. To overcome these limitations, great efforts are directed ...

Abstract	One of the challenges of science in disease prevention is optimizing drug and vaccine delivery. Until now, many strategies have been employed in this sector, but most are quite complex and labile. To overcome these limitations, great efforts are directed to coupling drugs to carriers, either of natural or synthetic origin. Among the most studied cell carriers are antigen-presenting cells (APCs), however, red blood cells (RBCs) are positioned as attractive carriers in drug delivery due to their abundance and availability in the body. Furthermore, fish RBCs have a nucleus and have been shown to have a strong involvement in modulating the immune response. In this study, we evaluated the binding of three peptides to rainbow trout RBCs, two lectin-like peptides and another derived from
MeSH term(s)	Animals ; Antiviral Agents/chemistry ; Drug Delivery Systems ; Erythrocytes/chemistry ; Fish Proteins/chemistry ; Lectins/chemistry ; Oncorhynchus mykiss ; Peptides/chemistry
Chemical Substances	Antiviral Agents ; Fish Proteins ; Lectins ; Peptides
Language	English
Publishing date	2021-10-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms222111821
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Antiviral Function of NKEF against VHSV in Rainbow Trout.

Chico, Veronica / Salvador-Mira, Maria Elizabhet / Nombela, Ivan / Puente-Marin, Sara / Perez, Luis / Mercado, Luis / Ortega-Villaizan, Maria Del Mar

Biology

2021 Volume 10, Issue 10

Abstract: Natural killer enhancing factor (NKEF) belongs to the peroxiredoxin family of proteins, a group of antioxidants that has been extensively studied in mammals. Recently, we identified NKEF in the immunoprecipitated proteome of rainbow trout red blood cells ...

Abstract	Natural killer enhancing factor (NKEF) belongs to the peroxiredoxin family of proteins, a group of antioxidants that has been extensively studied in mammals. Recently, we identified NKEF in the immunoprecipitated proteome of rainbow trout red blood cells (RBCs) exposed to viral hemorrhagic septicemia virus (VHSV). In the present study, we evaluated the role of NKEF in the antiviral response of rainbow trout against VHSV by examining the expression profile of NKEF in VHSV-exposed RBCs and rainbow trout gonad-2 (RTG-2) cell line. We found an in vitro correlation between decreased VHSV replication and increased NKEF expression after RBCs were exposed to VHSV, however this was not found in RTG-2 cells where the infection highly increased and
Language	English
Publishing date	2021-10-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology10101045
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Antiviral Function of NKEF against VHSV in Rainbow Trout

Chico, Veronica / Salvador-Mira, Maria Elizabhet / Nombela, Ivan / Puente-Marin, Sara / Perez, Luis / Mercado, Luis / Ortega-Villaizan, Maria del Mar

Biology. 2021 Oct. 15, v. 10, no. 10

2021

Abstract	Natural killer enhancing factor (NKEF) belongs to the peroxiredoxin family of proteins, a group of antioxidants that has been extensively studied in mammals. Recently, we identified NKEF in the immunoprecipitated proteome of rainbow trout red blood cells (RBCs) exposed to viral hemorrhagic septicemia virus (VHSV). In the present study, we evaluated the role of NKEF in the antiviral response of rainbow trout against VHSV by examining the expression profile of NKEF in VHSV-exposed RBCs and rainbow trout gonad-2 (RTG-2) cell line. We found an in vitro correlation between decreased VHSV replication and increased NKEF expression after RBCs were exposed to VHSV, however this was not found in RTG-2 cells where the infection highly increased and nkef transcripts remained almost unchanged. In addition, siRNA silencing of the nkef gene in rainbow trout RBCs and RTG-2 cells resulted in increased VHSV replication. We also found a correlation between nkef gene silencing and a decrease in the expression of genes related to type 1 interferon (IFN1) pathway. These findings indicated that NKEF is involved in the antiviral mechanisms of rainbow trout RBCs against VHSV and thus support its antiviral role and implication in the modulation of their immune response. Finally, overexpression of NKEF in an EPC cell line significantly reduced VHSV infectivity and was coupled to an increment in IFN1-related genes. In conclusion, NKEF may be a potential target for new therapeutic strategies against viral infections.
Keywords	Oncorhynchus mykiss ; Viral hemorrhagic septicemia virus ; cell lines ; genes ; immune response ; interferons ; pathogenicity ; peroxiredoxin ; proteome ; therapeutics
Language	English
Dates of publication	2021-1015
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology10101045
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: How modular protein nanoparticles may expand the ability of subunit anti-viral vaccines: The spring viremia carp virus (SVCV) case.

Rojas-Peña, Mauricio / Aceituno, Patricia / Salvador, Maria E / Garcia-Ordoñez, Marlid / Teles, Mariana / Ortega-Villaizan, Maria Del Mar / Perez, Luis / Roher, Nerea

Fish & shellfish immunology

2022 Volume 131, Page(s) 1051–1062

Abstract: Spring viremia of carp (SVC) remains as a vaccine orphan disease mostly affecting juvenile specimens. Young fish are especially difficult to vaccinate and oral administration of vaccine combined with food would be the election system to minimise stress ... ...

Abstract	Spring viremia of carp (SVC) remains as a vaccine orphan disease mostly affecting juvenile specimens. Young fish are especially difficult to vaccinate and oral administration of vaccine combined with food would be the election system to minimise stress and the vaccination costs associated to injection. However, administration of prophylactics with food pellets faces off several drawbacks mainly related with vaccine degradation and weak protection correlates of oral vaccines. Here we present a platform based on recombinant proteins (subunit vaccines) manufactured as highly resistant nanostructured materials, and providing excellent levels of protection against SVC virus in a preliminary i.p injection challenge. The G3 domain of SVCV glycoprotein G was overexpressed in E. coli together with IFNγ and the modular protein was purified from bacterial aggregates (inclusion bodies) as highly organised nanostructured biomaterial (nanopellets, NP). These SVCV-IFN
Language	English
Publishing date	2022-11-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2022.10.067
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 5917: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED