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  1. Article ; Online: Active surveillance of antimicrobial resistance.

    Oteo-Iglesias, Jesús

    Enfermedades infecciosas y microbiologia clinica (English ed.)

    2019  Volume 37 Suppl 1, Page(s) 26–31

    Abstract: The magnitude of the impact of antibiotic resistance (AR) is now well recognised. Implementation of measures for its control is essential, but for them to be efficacious and cost-effective, they must be guided by prior knowledge of the problem that is as ...

    Title translation Vigilancia activa de la resistencia a antibióticos.
    Abstract The magnitude of the impact of antibiotic resistance (AR) is now well recognised. Implementation of measures for its control is essential, but for them to be efficacious and cost-effective, they must be guided by prior knowledge of the problem that is as up-to-date, real and complete as possible. However, the current scale of AR is difficult to quantify due to the wide heterogeneity of data collection, the existence of large gaps in surveillance capacity and its deficient coordination. Therefore, it is necessary to structure holistic, integrative and high-quality AR surveillance systems that allow the early analysis of combined data (micro-biological, clinical, epidemiological data) from different sources (humans, foods, animals and environment) to guide decision making for the control of AR. Some of the main characteristics that a structure of this scale should have are: a multidisciplinary consensus on protocols, criteria and techniques; the combination and coordination of different approaches seeking to fill possible gaps in surveillance; the deve lopment of a global antibiotic policy that allows and facilitates the existence of common, updated and accessible surveillance systems; the use of agile and compatible computer systems that allow a «real time» dump in common databases; and political involvement to include monitoring of AR in the political agenda and to commit the necessary resources. Supplement information: This article is part of a supplement entitled «SEIMC External Quality Control Programme. Year 2016», which is sponsored by Roche, Vircell Microbiologists, Abbott Molecular and Francisco Soria Melguizo, S.A. © 2019 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosasy Microbiología Clínica. All rights reserved.
    MeSH term(s) Antimicrobial Stewardship/methods ; Antimicrobial Stewardship/organization & administration ; Antimicrobial Stewardship/standards ; Guidelines as Topic ; Humans
    Language Spanish
    Publishing date 2019-10-18
    Document type Journal Article
    ISSN 2529-993X
    ISSN (online) 2529-993X
    DOI 10.1016/S0213-005X(19)30179-X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Prevalence of

    Loras, Cristina / Pérez-Vázquez, María / González Prieto, Andrea / Oteo-Iglesias, Jesús / Alós, Juan-Ignacio

    Microbial drug resistance (Larchmont, N.Y.)

    2023  Volume 29, Issue 10, Page(s) 444–447

    Abstract: Plasmid-mediated resistance to fosfomycin has been seldom described ... ...

    Abstract Plasmid-mediated resistance to fosfomycin has been seldom described in
    Language English
    Publishing date 2023-04-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1290490-9
    ISSN 1931-8448 ; 1076-6294
    ISSN (online) 1931-8448
    ISSN 1076-6294
    DOI 10.1089/mdr.2022.0269
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: What's new in mechanisms of antibiotic resistance in bacteria of clinical origin?

    Asenjo, Alejandra / Oteo-Iglesias, Jesús / Alós, Juan-Ignacio

    Enfermedades infecciosas y microbiologia clinica (English ed.)

    2021  Volume 39, Issue 6, Page(s) 291–299

    Abstract: The discovery, commercialization and administration of antibiotics revolutionized the world of medicine in the middle of the last century, generating a significant change in the therapeutic paradigm of the infectious diseases. Nevertheless, this great ... ...

    Abstract The discovery, commercialization and administration of antibiotics revolutionized the world of medicine in the middle of the last century, generating a significant change in the therapeutic paradigm of the infectious diseases. Nevertheless, this great breakthrough was soon threatened due to the enormous adaptive ability that bacteria have, through which they are able to develop or acquire different mechanisms that allow them to survive the exposure to antibiotics. We are faced with a complex, multifactorial and inevitable but potentially manageable threat. To fight against it, a global and multidisciplinary approach is necessary, based on the support, guidance and training of the next generation of professionals. Nevertheless, the information published regarding the resistance mechanisms to antibiotics are abundant, varied and, unfortunately, not always well structured. The objective of this review is to structure the, in our opinion, most relevant and novel information regarding the mechanisms of resistance to antibiotics that has been published from January 2014 to September 2019, analysing their possible clinical and epidemiological impact.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Bacteria ; Drug Resistance, Microbial
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2021-05-04
    Document type Journal Article ; Review
    ISSN 2529-993X
    ISSN (online) 2529-993X
    DOI 10.1016/j.eimce.2020.02.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Community Emergence of Cefixime-Resistant

    Zaragoza, Gloria / Pérez-Vázquez, María / Villar-Gómara, Laura / González-Prieto, Andrea / Oteo-Iglesias, Jesús / Alós, Juan-Ignacio

    Antibiotics (Basel, Switzerland)

    2024  Volume 13, Issue 3

    Abstract: Escherichia ... ...

    Abstract Escherichia coli
    Language English
    Publishing date 2024-02-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics13030218
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Genomic Surveillance Uncovers a 10-Year Persistence of an OXA-24/40

    Aranzamendi, Maitane / Xanthopoulou, Kyriaki / Sánchez-Urtaza, Sandra / Burgwinkel, Tessa / Arazo Del Pino, Rocío / Lucaßen, Kai / Pérez-Vázquez, M / Oteo-Iglesias, Jesús / Sota, Mercedes / Marimón, Jose María / Seifert, Harald / Higgins, Paul G / Gallego, Lucía

    International journal of molecular sciences

    2024  Volume 25, Issue 4

    Abstract: Infections caused by carbapenem- ... ...

    Abstract Infections caused by carbapenem-resistant
    MeSH term(s) Multilocus Sequence Typing ; Bacterial Proteins/genetics ; Tertiary Care Centers ; Acinetobacter baumannii/genetics ; Spain/epidemiology ; Microbial Sensitivity Tests ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Genomics ; beta-Lactamases/genetics
    Chemical Substances Bacterial Proteins ; Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2024-02-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25042333
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Characterization of an outbreak caused by Elizabethkingia miricola using Fourier-transform infrared (FTIR) spectroscopy.

    Rodríguez-Temporal, David / García-Cañada, Javier Enrique / Candela, Ana / Oteo-Iglesias, Jesús / Serrano-Lobo, Julia / Pérez-Vázquez, María / Rodríguez-Sánchez, Belén / Cercenado, Emilia

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

    2024  Volume 43, Issue 4, Page(s) 797–803

    Abstract: Fourier-transform infrared (FTIR) spectroscopy has the potential to be used for bacterial typing and outbreak characterization. We evaluated FTIR for the characterization of an outbreak caused by Elizabethkingia miricola. During the 2020-2021 period, 26 ... ...

    Abstract Fourier-transform infrared (FTIR) spectroscopy has the potential to be used for bacterial typing and outbreak characterization. We evaluated FTIR for the characterization of an outbreak caused by Elizabethkingia miricola. During the 2020-2021 period, 26 isolates (23 clinical and 3 environmental) were collected and analyzed by FTIR (IR Biotyper) and core-genome MLST (cgMLST), in addition to antimicrobial susceptibility testing. FTIR spectroscopy and cgMLST showed that 22 of the isolates were related to the outbreak, including the environmental samples, with only one discordance between both methods. Then, FTIR is useful for E. miricola typing and can be easily implemented in the laboratory.
    MeSH term(s) Humans ; Multilocus Sequence Typing ; Spectroscopy, Fourier Transform Infrared ; Flavobacteriaceae/genetics ; Disease Outbreaks
    Language English
    Publishing date 2024-02-14
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 603155-9
    ISSN 1435-4373 ; 0934-9723 ; 0722-2211
    ISSN (online) 1435-4373
    ISSN 0934-9723 ; 0722-2211
    DOI 10.1007/s10096-024-04764-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: In vitro activity of six biocides against carbapenemase-producing Klebsiella pneumoniae and presence of genes encoding efflux pumps.

    Gual-de-Torrella, Ana / Delgado-Valverde, Mercedes / Pérez-Palacios, Patricia / Oteo-Iglesias, Jesús / Pascual, Álvaro / Fernández-Cuenca, Felipe

    Enfermedades infecciosas y microbiologia clinica (English ed.)

    2022  Volume 40, Issue 7, Page(s) 371–376

    Abstract: Introduction: Acquisition of reduced susceptibility to biocides may contribute to the dissemination of high-risk (HR) clones of carbapenemase-producing Klebsiella pneumoniae (CP-Kp). The aim of this study was (a) to determinate the activity of biocides ... ...

    Abstract Introduction: Acquisition of reduced susceptibility to biocides may contribute to the dissemination of high-risk (HR) clones of carbapenemase-producing Klebsiella pneumoniae (CP-Kp). The aim of this study was (a) to determinate the activity of biocides against CP-Kp, and (b) to analyse the relationship between biocide activity and the presence of efflux pumps.
    Methods: The minimal inhibitory concentrations (MICs) of 6 biocides (sodium hypochlorite, chlorhexidine digluconate, benzalkonium chloride, povidone-iodine, ethanol and triclosan) were determined in triplicate at 25°C and 37°C in Mueller-Hinton broth (MHB) and M9 minimum medium, against 17 CP-Kp isolates representing different clones (HR and no-HR), sequence-types (STs) and carbapenemases. Efflux pumps genes were detected by whole genome sequencing (MiSeq).
    Results: Median MICs were slightly higher at 37°C than at 25°C (p≤0.05), except for benzalkonium chloride, triclosan and ethanol. MIC medians were much higher in MHB than in M9, except for triclosan. No significant differences were observed in the median MICs, regarding the type of clone, ST or carbapenemase; cepA, acrAB, kpnEF and oqxAB genes were detected in all isolates, whereas qacE and qacA were not detected; smvAR, and qacΔE genes were detected in 94% and 47% of isolates, respectively.
    Conclusions: Triclosan, chlorhexidine digluconate, benzalkonium chloride and ethanol were the most active biocides. The activity of some biocides is affected by temperature and growth media, suggesting that standardised procedures for biocide susceptibility testing based on MIC determination are required. This activity, in terms of MICs, are not related to the type of clone, ST, carbapenemase or the presence of the efflux pump genes.
    MeSH term(s) Bacterial Proteins ; Benzalkonium Compounds/pharmacology ; Carbapenem-Resistant Enterobacteriaceae ; Disinfectants/pharmacology ; Ethanol ; Klebsiella pneumoniae/genetics ; Triclosan ; beta-Lactamases
    Chemical Substances Bacterial Proteins ; Benzalkonium Compounds ; Disinfectants ; Ethanol (3K9958V90M) ; Triclosan (4NM5039Y5X) ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
    Language English
    Publishing date 2022-07-26
    Publishing country Spain
    Document type Journal Article
    ISSN 2529-993X
    ISSN (online) 2529-993X
    DOI 10.1016/j.eimce.2021.05.016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Transfer of plasmids harbouring blaOXA-48-like carbapenemase genes in biofilm-growing Klebsiella pneumoniae: Effect of biocide exposure

    Pérez-Palacios, Patricia / Gual-de-Torrella, Ana / Delgado-Valverde, Mercedes / Oteo-Iglesias, Jesús / Hidalgo-Díaz, Carmen / Pascual, Álvaro / Fernández-Cuenca, Felipe

    Microbiological research. 2022 Jan., v. 254

    2022  

    Abstract: The spread of OXA-48-encoding plasmids from Klebsiella pneumoniae (OXA-48-Kpn), especially successful high-risk (HR) clones, is a growing concern. Biofilm formation can contribute to the dissemination of OXA-48-Kpn. It is not known whether biocides can ... ...

    Abstract The spread of OXA-48-encoding plasmids from Klebsiella pneumoniae (OXA-48-Kpn), especially successful high-risk (HR) clones, is a growing concern. Biofilm formation can contribute to the dissemination of OXA-48-Kpn. It is not known whether biocides can affect the transfer of OXA-48-Kpn in biofilm. The aim of this study was to evaluate the effect of biocides on the conjugation frequency (CF) of OXA-48-Kpn in both biofilm and planktonic cultures. For that, seven OXA-48-Kpn isolates (4 belonging to HR clones and 3 to non-HR clones) were selected as donors. Each isolate was mixed (1:1) with Escherichia coli J53 (recipient) and grown on polystyrene microplates without biocides (control) and with 0.25x MIC of triclosan (TRI), chlorhexidine digluconate (CHX), povidone-iodine (POV), sodium hypochlorite (SOD) or ethanol (ETH). The CF was calculated as the number of transconjugants/number of E. coli J53. The results showed that for isolates growing in the absence of biocide, the mean fold change in the CF in biofilm with respect to that determined in planktonic cells (CF-BF/CF-PK) was 0.2 in non-HR isolates and ranged from 2.0 to 14.7 in HR isolates. In HR isolates grown in the presence of biocide, especially CHX, TRI, and ETH, the fold changes in CF-BF/CF-PK decreased, whereas in non-HR isolates the fold changes were similar or increased slightly with CHX, ETH, SOD and POV. In conclusion, the fold changes in the CF-BF/CF-PK are higher in HR isolates comparing to non-HR isolates in abscence of biocides. The fold changes in CF-BF/CF-PK of the HR isolates in the presence of biocides varied with the type of biocides, whereas in non-HR isolates, biocides have no significant effect, or produce only a slight increase in the fold change of CF-BF/CF-PK.
    Keywords Escherichia coli ; Klebsiella pneumoniae ; beta-lactamase ; biocides ; biofilm ; chlorhexidine ; ethanol ; plankton ; plasmids ; polystyrenes ; research ; sodium hypochlorite ; triclosan
    Language English
    Dates of publication 2022-01
    Publishing place Elsevier GmbH
    Document type Article
    ZDB-ID 1189614-0
    ISSN 1618-0623 ; 0944-5013
    ISSN (online) 1618-0623
    ISSN 0944-5013
    DOI 10.1016/j.micres.2021.126894
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Pseudomonas aeruginosa

    Sastre-Femenia, Miquel Àngel / Fernández-Muñoz, Almudena / Gomis-Font, María Antonia / Taltavull, Biel / López-Causapé, Carla / Arca-Suárez, Jorge / Martínez-Martínez, Luis / Cantón, Rafael / Larrosa, Nieves / Oteo-Iglesias, Jesús / Zamorano, Laura / Oliver, Antonio

    The Lancet regional health. Europe

    2023  Volume 34, Page(s) 100736

    Abstract: Background: Pseudomonas aeruginosa: Methods: A total of 3.180 nonduplicated : Findings: A decrease in resistance to all tested antibiotics, including older and newer antimicrobials, was observed in 2022 vs 2017. Likewise, a major reduction of XDR ( ...

    Abstract Background: Pseudomonas aeruginosa
    Methods: A total of 3.180 nonduplicated
    Findings: A decrease in resistance to all tested antibiotics, including older and newer antimicrobials, was observed in 2022 vs 2017. Likewise, a major reduction of XDR (15.2% vs 5.9%) and DTR (4.2 vs 2.1%) profiles was evidenced, and even more patent among ICU isolates [XDR (26.0% vs 6.0%) and DTR (8.9% vs 2.6%)] (p < 0.001). The prevalence of Extended-spectrum β-lactamase/carbapenemase production was slightly lower in 2022 (2.1%. vs 3.1%, p = 0.064). However, there was a significant increase in the proportion of carbapenemase production among carbapenem-resistant strains (29.4% vs 18.1%, p = 0.0246). While ST175 was still the most frequent clone among XDR, a slight reduction in its prevalence was noted (35.9% vs 45.5%, p = 0.106) as opposed to ST235 which increased significantly (24.3% vs 12.3%, p = 0.0062).
    Interpretation: While the generalized decrease in
    Funding: MSD and the Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea-NextGenerationEU.
    Language English
    Publishing date 2023-09-19
    Publishing country England
    Document type Journal Article
    ISSN 2666-7762
    ISSN (online) 2666-7762
    DOI 10.1016/j.lanepe.2023.100736
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Mutation Analysis in Regulator DNA-Binding Regions for Antimicrobial Efflux Pumps in 17,000

    Pérez-Vázquez, María / López-Causapé, Carla / Corral-Lugo, Andrés / McConnell, Michael J / Oteo-Iglesias, Jesús / Oliver, Antonio / Martín-Galiano, Antonio J

    Microorganisms

    2023  Volume 11, Issue 10

    Abstract: Mutations leading to upregulation of efflux pumps can produce multiple drug resistance in the ... ...

    Abstract Mutations leading to upregulation of efflux pumps can produce multiple drug resistance in the pathogen
    Language English
    Publishing date 2023-10-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11102486
    Database MEDical Literature Analysis and Retrieval System OnLINE

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