LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 208

Search options

  1. Article ; Online: Tension Hemopneumothorax in the Setting of Mechanical CPR during Prehospital Cardiac Arrest.

    Rowland, Dustin / Vryhof, Nicholas / Overton, David / Mastenbrook, Joshua

    Prehospital emergency care : official journal of the National Association of EMS Physicians and the National Association of State EMS Directors

    2020  Volume 25, Issue 2, Page(s) 274–280

    Abstract: Introduction: There are several complications associated with automated mechanical CPR (AM-CPR), including tension pneumothoraces. The incidence of these complications and the risk factors for their development remain poorly characterized. Tension ... ...

    Abstract Introduction: There are several complications associated with automated mechanical CPR (AM-CPR), including tension pneumothoraces. The incidence of these complications and the risk factors for their development remain poorly characterized. Tension hemopneumothorax is a previously unreported complication of AM-CPR. The authors present a case of a suspected tension hemopneumothorax that developed during the use of an automated mechanical CPR device.
    MeSH term(s) Aged ; Cardiopulmonary Resuscitation ; Emergency Medical Services ; Female ; Heart Arrest/etiology ; Heart Arrest/therapy ; Hemopneumothorax/etiology ; Hemopneumothorax/therapy ; Humans ; Pneumothorax/etiology ; Pneumothorax/therapy
    Language English
    Publishing date 2020-04-14
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 1461751-1
    ISSN 1545-0066 ; 1090-3127
    ISSN (online) 1545-0066
    ISSN 1090-3127
    DOI 10.1080/10903127.2020.1743800
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4762: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Generation of Drosophila attP containing cell lines using CRISPR-Cas9.

    Mariyappa, Daniel / Luhur, Arthur / Overton, Danielle / Zelhof, Andrew C

    G3 (Bethesda, Md.)

    2021  Volume 11, Issue 8

    Abstract: The generation of Drosophila stable cell lines has become invaluable for complementing in vivo experiments and as tools for genetic screens. Recent advances utilizing attP/PhiC31 integrase system has permitted the creation of Drosophila cells in which ... ...

    Abstract The generation of Drosophila stable cell lines has become invaluable for complementing in vivo experiments and as tools for genetic screens. Recent advances utilizing attP/PhiC31 integrase system has permitted the creation of Drosophila cells in which recombination mediated cassette exchange (RMCE) can be utilized to generate stably integrated transgenic cell lines that contain a single copy of the transgene at the desired locus. Current techniques, besides being laborious and introducing extraneous elements, are limited to a handful of cell lines of embryonic origin. Nonetheless, with well over 100 Drosophila cell lines available, including an ever-increasing number CRISPR/Cas9 modified cell lines, a more universal methodology is needed to generate a stably integrated transgenic line from any one of the available Drosophila melanogaster cell lines. Here, we describe a toolkit and procedure that combines CRISPR/Cas9 and theaaa PhiC31 integrase system. We have generated and isolated single cell clones containing an Actin5C::dsRed cassette flanked by attP sites into the genome of Kc167 and S2R+ cell lines that mimic the in vivo attP sites located at 25C6 and 99F8 of the Drosophila genome. Furthermore, we tested the functionality of the attP docking sites utilizing two independent GFP expressing constructs flanked by attB sites that permit RMCE and therefore the insertion of any DNA of interest. Lastly, to demonstrate the universality of our methodology and existing constructs, we have successfully integrated the Actin5C::dsRed cassette flanked by attP sites into two different CNS cell lines, ML-DmBG2-c2 and ML-DmBG3-c2. Overall, the reagents and methodology reported here permit the efficient generation of stable transgenic cassettes with minimal change in the cellular genomes in existing D. melanogaster cell lines.
    MeSH term(s) Animals ; CRISPR-Cas Systems ; Cell Line ; Drosophila/genetics ; Drosophila/metabolism ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Integrases/genetics ; Integrases/metabolism
    Chemical Substances Integrases (EC 2.7.7.-)
    Language English
    Publishing date 2021-05-21
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1093/g3journal/jkab161
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: "You did a great job building that!" Links between parent-child prosocial talk and spatial language.

    Hall, LaTreese V / Rengel, Melanie / Bowley, Hannah / Alvarez-Vargas, Daniela / Abad, Carla / Overton, Dyamond / Pruden, Shannon M

    Developmental psychology

    2023  Volume 59, Issue 9, Page(s) 1676–1690

    Abstract: We investigated the extent to which parents' prosocial talk and negations relate to the quantity and diversity of parents' spatial language production. We also examined similar associations among children. Participants included 51 children of ages 4-7 ... ...

    Abstract We investigated the extent to which parents' prosocial talk and negations relate to the quantity and diversity of parents' spatial language production. We also examined similar associations among children. Participants included 51 children of ages 4-7 years and their parents recruited from South Florida. Most of the dyads included mothers and were Hispanic and bilingual. Dyads constructed a Lego house for 10 min. Sessions were transcribed and coded for instances of parent prosocial talk (praises, reflective statements, and behavior descriptions), child general positive statements (all positive contributions to the interaction), and parent and child negations (criticisms, corrections, and disapprovals) using the Dyadic Parent-Child Interaction Coding System. Transcripts were also coded for quantity and diversity of spatial language including shape terms (e.g.,
    MeSH term(s) Female ; Humans ; Parent-Child Relations ; Language ; Parents ; Mothers ; Language Development
    Language English
    Publishing date 2023-07-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2066223-3
    ISSN 1939-0599 ; 0012-1649
    ISSN (online) 1939-0599
    ISSN 0012-1649
    DOI 10.1037/dev0001574
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Book ; Online: Small business server 2008

    Overton, David

    installation, migration, and configuration ; set up and run your small business server making it deliver big business impact

    (From technologies to solutions)

    2009  

    Abstract: Set up and run Microsoft Small Business Server 2008 making it deliver a big business impact with this book and ... ...

    Institution ebrary, Inc
    Author's details David Overton
    Series title From technologies to solutions
    Abstract Set up and run Microsoft Small Business Server 2008 making it deliver a big business impact with this book and eBook
    Keywords Client/server computing ; Systems migration
    Language English
    Size Online-Ressource (vii, 387 p), ill. (some col.)
    Publisher Packt Pub
    Publishing place Birmingham, U.K
    Document type Book ; Online
    Note Includes index
    ISBN 9781847196309 ; 9781847196316 ; 1847196306 ; 1847196314
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: A novel transposable element-based authentication protocol for Drosophila cell lines.

    Mariyappa, Daniel / Rusch, Douglas B / Han, Shunhua / Luhur, Arthur / Overton, Danielle / Miller, David F B / Bergman, Casey M / Zelhof, Andrew C

    G3 (Bethesda, Md.)

    2021  Volume 12, Issue 2

    Abstract: Drosophila cell lines are used by researchers to investigate various cell biological phenomena. It is crucial to exercise good cell culture practice. Poor handling can lead to both inter- and intra-species cross-contamination. Prolonged culturing can ... ...

    Abstract Drosophila cell lines are used by researchers to investigate various cell biological phenomena. It is crucial to exercise good cell culture practice. Poor handling can lead to both inter- and intra-species cross-contamination. Prolonged culturing can lead to introduction of large- and small-scale genomic changes. These factors, therefore, make it imperative that methods to authenticate Drosophila cell lines are developed to ensure reproducibility. Mammalian cell line authentication is reliant on short tandem repeat (STR) profiling; however, the relatively low STR mutation rate in Drosophila melanogaster at the individual level is likely to preclude the value of this technique. In contrast, transposable elements (TEs) are highly polymorphic among individual flies and abundant in Drosophila cell lines. Therefore, we investigated the utility of TE insertions as markers to discriminate Drosophila cell lines derived from the same or different donor genotypes, divergent sub-lines of the same cell line, and from other insect cell lines. We developed a PCR-based next-generation sequencing protocol to cluster cell lines based on the genome-wide distribution of a limited number of diagnostic TE families. We determined the distribution of five TE families in S2R+, S2-DRSC, S2-DGRC, Kc167, ML-DmBG3-c2, mbn2, CME W1 Cl.8+, and ovarian somatic sheath Drosophila cell lines. Two independent downstream analyses of the next-generation sequencing data yielded similar clustering of these cell lines. Double-blind testing of the protocol reliably identified various Drosophila cell lines. In addition, our data indicate minimal changes with respect to the genome-wide distribution of these five TE families when cells are passaged for at least 50 times. The protocol developed can accurately identify and distinguish the numerous Drosophila cell lines available to the research community, thereby aiding reproducible Drosophila cell culture research.
    MeSH term(s) Animals ; Cell Line ; DNA Transposable Elements/genetics ; Drosophila/genetics ; Drosophila melanogaster/genetics ; Genome, Insect ; Reproducibility of Results
    Chemical Substances DNA Transposable Elements
    Language English
    Publishing date 2021-11-25
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1093/g3journal/jkab403
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article: STATE-DEPENDENT OR "DISSOCIATED" LEARNING PRODUCED WITH PENTOBARBITAL.

    OVERTON, D A

    Journal of comparative and physiological psychology

    2003  Volume 57, Page(s) 3–12

    MeSH term(s) Avoidance Learning ; Conditioning (Psychology) ; Gallamine Triethiodide ; Learning ; Pentobarbital ; Pharmacology ; Rats ; Research ; Tetraethylammonium Compounds
    Chemical Substances Tetraethylammonium Compounds ; Pentobarbital (I4744080IR) ; Gallamine Triethiodide (Q3254X40X2)
    Language English
    Publishing date 2003-09-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3146-x
    ISSN 0021-9940
    ISSN 0021-9940
    DOI 10.1037/h0048023
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1231: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Financial Incentives for Medical Assistants: A Mixed-Methods Exploration of Bonus Structures, Motivation, and Population Health Quality Measures.

    Vilendrer, Stacie / Brown-Johnson, Cati / Kling, Samantha M R / Veruttipong, Darlene / Amano, Alexis / Bohman, Bryan / Daines, William P / Overton, David / Srivastava, Raj / Asch, Steven M

    Annals of family medicine

    2021  Volume 19, Issue 5, Page(s) 427–436

    Abstract: Purpose: Medical assistants (MAs) have seen their roles expand as a result of team-based primary care models. Unlike their physician counterparts, MAs rarely receive financial incentives as a part of their compensation. This exploratory study aims to ... ...

    Abstract Purpose: Medical assistants (MAs) have seen their roles expand as a result of team-based primary care models. Unlike their physician counterparts, MAs rarely receive financial incentives as a part of their compensation. This exploratory study aims to understand MA acceptability of financial incentives and perceived MA control over common population health measures.
    Methods: We conducted semistructured focus groups between August and December of 2019 across 10 clinics affiliated with 3 institutions in California and Utah. MAs' perceptions of experienced and hypothetical financial incentives, their potential influence on workflow processes, and perceived levels of control over population health measures were discussed, recorded, and qualitatively analyzed for emerging themes. Perceived levels of control were further quantified using a Likert survey; measures were grouped into factors representing vaccinations, and workflow completed in the same day or multiple days (multiday
    Results: MAs reported little direct experience with financial incentives. They indicated that a hypothetical bonus representing 2% to 3% of their average annual base pay would be acceptable and influential in improving consistent performance during patient rooming workflow. MAs reported having greater perceived control over vaccinations (
    Conclusions: MAs perceived that relatively small financial incentives would increase their motivation and quality of care. Our findings suggests target measures should focus on MA work processes that are completed in the same day as the patient encounter, particularly vaccinations. Future investigation is needed to understand the effectiveness of MA financial incentives in practice.
    MeSH term(s) Humans ; Motivation ; Population Health ; Primary Health Care ; Quality Indicators, Health Care ; Surveys and Questionnaires
    Language English
    Publishing date 2021-09-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2171425-3
    ISSN 1544-1717 ; 1544-1709
    ISSN (online) 1544-1717
    ISSN 1544-1709
    DOI 10.1370/afm.2719
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6065: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: Turning quality-related activities into quality research.

    Overton, D T

    Academic emergency medicine : official journal of the Society for Academic Emergency Medicine

    1995  Volume 2, Issue 2, Page(s) 139–143

    MeSH term(s) Emergency Medicine/education ; Emergency Medicine/standards ; Health Services Research ; Quality of Health Care ; United States
    Language English
    Publishing date 1995-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1329813-6
    ISSN 1553-2712 ; 1069-6563
    ISSN (online) 1553-2712
    ISSN 1069-6563
    DOI 10.1111/j.1553-2712.1995.tb03180.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4479: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article: Quality-related programs.

    Overton, D T

    Academic emergency medicine : official journal of the Society for Academic Emergency Medicine

    1994  Volume 1, Issue 2, Page(s) 192–194

    MeSH term(s) Emergency Medicine/standards ; Humans ; Total Quality Management
    Language English
    Publishing date 1994-03
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1329813-6
    ISSN 1553-2712 ; 1069-6563
    ISSN (online) 1553-2712
    ISSN 1069-6563
    DOI 10.1111/j.1553-2712.1994.tb02761.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4479: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article: A historical perspective on drug discrimination.

    Overton, D A

    NIDA research monograph

    1991  , Issue 116, Page(s) 5–24

    MeSH term(s) Animals ; Cues ; Discrimination Learning/drug effects ; Humans ; Mental Recall
    Language English
    Publishing date 1991
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S. ; Review
    ISSN 1046-9516
    ISSN 1046-9516
    DOI 10.1037/e496182006-002
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top