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  1. Article: Multiplex Real-Time PCR Assays for the Identification of the Potato Cyst and Tobacco Cyst Nematodes.

    Nakhla, Mark K / Owens, Kristina J / Li, Wenbin / Wei, Gang / Skantar, Andrea M / Levy, Laurene

    Plant disease

    2019  Volume 94, Issue 8, Page(s) 959–965

    Abstract: TaqMan primer-probe sets were developed for the detection and identification of potato cyst nematodes (PCNs) Globodera pallida and G. rostochiensis using two-tube, multiplex real-time polymerase chain reaction (PCR). One tube contained a primer-probe set ...

    Abstract TaqMan primer-probe sets were developed for the detection and identification of potato cyst nematodes (PCNs) Globodera pallida and G. rostochiensis using two-tube, multiplex real-time polymerase chain reaction (PCR). One tube contained a primer-probe set specific for G. pallida (pale potato cyst nematode) multiplexed with another primer-probe set specific for G. rostochiensis (golden potato cyst nematode). A second tube consisted of the G. pallida-specific primer-probe set multiplexed with a primer-probe set specific for G. tabacum (the morphologically similar tobacco cyst nematode). This internal transcribed spacer rDNA-based system was specific for the Globodera spp. of interest and successfully identified several populations of PCN. This rapid, sensitive, and specific quantitative PCR assay presents a useful tool for PCN regulatory response and management programs.
    Language English
    Publishing date 2019-02-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-94-8-0959
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  2. Article: Multiplex Real-Time PCR Assays for the Identification of the Potato Cyst and Tobacco Cyst Nematodes

    Nakhla, Mark K / Owens, Kristina J / Li, Wenbin / Wei, Gang / Skantar, Andrea M / Levy, Laurene

    Plant disease. 2010 Aug., v. 94, no. 8

    2010  

    Abstract: TaqMan primer-probe sets were developed for the detection and identification of potato cyst nematodes (PCNs) Globodera pallida and G. rostochiensis using two-tube, multiplex real-time polymerase chain reaction (PCR). One tube contained a primer-probe set ...

    Abstract TaqMan primer-probe sets were developed for the detection and identification of potato cyst nematodes (PCNs) Globodera pallida and G. rostochiensis using two-tube, multiplex real-time polymerase chain reaction (PCR). One tube contained a primer-probe set specific for G. pallida (pale potato cyst nematode) multiplexed with another primer-probe set specific for G. rostochiensis (golden potato cyst nematode). A second tube consisted of the G. pallida-specific primer-probe set multiplexed with a primer-probe set specific for G. tabacum (the morphologically similar tobacco cyst nematode). This internal transcribed spacer rDNA-based system was specific for the Globodera spp. of interest and successfully identified several populations of PCN. This rapid, sensitive, and specific quantitative PCR assay presents a useful tool for PCN regulatory response and management programs.
    Keywords Globodera pallida ; Globodera rostochiensis ; Globodera tabacum ; plant pests ; cyst nematodes ; pest identification ; polymerase chain reaction ; ribosomal DNA ; internal transcribed spacers ; rapid methods ; quantitative analysis
    Language English
    Dates of publication 2010-08
    Size p. 959-965.
    Document type Article
    Note Includes references
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-94-8-0959
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  3. Article ; Online: The U.S. Culture Collection Network Responding to the Requirements of the Nagoya Protocol on Access and Benefit Sharing.

    McCluskey, Kevin / Barker, Katharine B / Barton, Hazel A / Boundy-Mills, Kyria / Brown, Daniel R / Coddington, Jonathan A / Cook, Kevin / Desmeth, Philippe / Geiser, David / Glaeser, Jessie A / Greene, Stephanie / Kang, Seogchan / Lomas, Michael W / Melcher, Ulrich / Miller, Scott E / Nobles, David R / Owens, Kristina J / Reichman, Jerome H / da Silva, Manuela /
    Wertz, John / Whitworth, Cale / Smith, David

    mBio

    2017  Volume 8, Issue 4

    Abstract: The U.S. Culture Collection Network held a meeting to share information about how culture collections are responding to the requirements of the recently enacted Nagoya Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits ...

    Abstract The U.S. Culture Collection Network held a meeting to share information about how culture collections are responding to the requirements of the recently enacted Nagoya Protocol on Access to Genetic Resources and the Fair and Equitable Sharing of Benefits Arising from their Utilization to the Convention on Biological Diversity (CBD). The meeting included representatives of many culture collections and other biological collections, the U.S. Department of State, U.S. Department of Agriculture, Secretariat of the CBD, interested scientific societies, and collection groups, including Scientific Collections International and the Global Genome Biodiversity Network. The participants learned about the policies of the United States and other countries regarding access to genetic resources, the definition of genetic resources, and the status of historical materials and genetic sequence information. Key topics included what constitutes access and how the CBD Access and Benefit-Sharing Clearing-House can help guide researchers through the process of obtaining Prior Informed Consent on Mutually Agreed Terms. U.S. scientists and their international collaborators are required to follow the regulations of other countries when working with microbes originally isolated outside the United States, and the local regulations required by the Nagoya Protocol vary by the country of origin of the genetic resource. Managers of diverse living collections in the United States described their holdings and their efforts to provide access to genetic resources. This meeting laid the foundation for cooperation in establishing a set of standard operating procedures for U.S. and international culture collections in response to the Nagoya Protocol.
    MeSH term(s) Agriculture/legislation & jurisprudence ; Agriculture/organization & administration ; Biodiversity ; Biological Specimen Banks/legislation & jurisprudence ; Biological Specimen Banks/organization & administration ; Biotechnology/legislation & jurisprudence ; Biotechnology/organization & administration ; Databases, Genetic/legislation & jurisprudence ; Environmental Microbiology ; Models, Genetic ; United States ; United States Department of Agriculture
    Language English
    Publishing date 2017-08-15
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mBio.00982-17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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