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Article ; Online: In Response to p53 Immunohistochemical Staining and TP53 Gene Mutations in Endometrial Cancer: Does Null Pattern Correlate With Prognosis?

Sakamoto, Ikuko / Kagami, Keiko / Nozaki, Takahiro / Hirotsu, Yosuke / Amemiya, Kenji / Oyama, Toshio / Omata, Masao

The American journal of surgical pathology

2024 Volume 48, Issue 3, Page(s) 374–375

MeSH term(s)	Female ; Humans ; Tumor Suppressor Protein p53/genetics ; Genes, p53 ; Mutation ; Prognosis ; Endometrial Neoplasms/genetics
Chemical Substances	Tumor Suppressor Protein p53 ; TP53 protein, human
Language	English
Publishing date	2024-01-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	752964-8
ISSN	1532-0979 ; 0147-5185
ISSN (online)	1532-0979
ISSN	0147-5185
DOI	10.1097/PAS.0000000000002181
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Zs.A 1356: Show issues

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Article ; Online: Mixed micropapillary patterns found in malignant pleural mesothelioma with possibly worsened prognostic implication.

Oyama, Toshio / Goto, Taichiro / Amemiya, Kenji

Thoracic cancer

2022 Volume 13, Issue 7, Page(s) 1098–1099

MeSH term(s)	Biomarkers, Tumor ; Humans ; Lung Neoplasms/pathology ; Mesothelioma/pathology ; Mesothelioma, Malignant ; Pleural Neoplasms/pathology ; Prognosis
Chemical Substances	Biomarkers, Tumor
Language	English
Publishing date	2022-02-24
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2625856-0
ISSN	1759-7714 ; 1759-7706
ISSN (online)	1759-7714
ISSN	1759-7706
DOI	10.1111/1759-7714.14364
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Zs.A 6921: Show issues

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Article ; Online: p53 Immunohistochemical Staining and TP53 Gene Mutations in Endometrial Cancer: Does Null Pattern Correlate With Prognosis?

Sakamoto, Ikuko / Kagami, Keiko / Nozaki, Takahiro / Hirotsu, Yosuke / Amemiya, Kenji / Oyama, Toshio / Omata, Masao

The American journal of surgical pathology

2023 Volume 47, Issue 10, Page(s) 1144–1150

Abstract: Whether immunohistochemistry (IHC) of p53 accurately reflects the TP53 mutational status of endometrial carcinoma (EC) has not yet been established. This study aimed to clarify the relationship between p53 IHC and TP53 mutations in EC and to examine ... ...

Abstract	Whether immunohistochemistry (IHC) of p53 accurately reflects the TP53 mutational status of endometrial carcinoma (EC) has not yet been established. This study aimed to clarify the relationship between p53 IHC and TP53 mutations in EC and to examine whether p53 IHC can be a more convenient prognostic marker than TP53 mutation in EC. We performed p53 IHC staining of EC samples obtained via surgery and genetic analyses using next-generation sequencing. p53 IHC results showed that of the 101 cases, 71 (70%) were wild-type (WT), 12 (12%) were overexpression (OE), and 18 (18%) were in the null group. Missense mutations were found in 9 cases (47.4%) in OE, 2 (10.5%) in null, and 8 (42.1%) in the WT group. Truncating mutations were found in 1 case (8.3%) in OE, 6 (50%) in null, and 5 (41.7%) in the WT group. The 5-year progression-free survival was 0% in OE, 74.8% in null, and 79.0% in the WT group. In the prognosis for each type of TP53 mutation, the 5-year progression-free survival was missense (32.2%), truncating (65.6%), and WT (79.7%). These survival comparisons showed that the p53 IHC OE had the poorest prognosis. These results suggest that the p53 IHC OE is an independent poor prognostic factor for EC and can be used as a simple and rapid surrogate marker for TP53 mutations. Contrastingly, the complete absence of p53 IHC-the null staining pattern-may not accurately predict a TP53 mutation in EC, and it is necessary to be more careful in making the diagnosis of "abnormal."
MeSH term(s)	Female ; Humans ; Tumor Suppressor Protein p53/genetics ; Genes, p53 ; Mutation ; Prognosis ; Endometrial Neoplasms/genetics ; Endometrial Neoplasms/pathology
Chemical Substances	Tumor Suppressor Protein p53 ; TP53 protein, human
Language	English
Publishing date	2023-08-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752964-8
ISSN	1532-0979 ; 0147-5185
ISSN (online)	1532-0979
ISSN	0147-5185
DOI	10.1097/PAS.0000000000002106
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Influence of formalin fixation duration on RNA quality and quantity from formalin-fixed paraffin-embedded hepatocellular carcinoma tissues.

Amemiya, Kenji / Hirotsu, Yosuke / Nagakubo, Yuki / Mochizuki, Hitoshi / Oyama, Toshio / Omata, Masao

Pathology international

2023 Volume 73, Issue 12, Page(s) 593–600

Abstract: Analyzing RNA samples from formalin-fixed paraffin-embedded (FFPE) tissues is essential for precision medicine. We investigated RNA quantity and quality from FFPE tumor tissues fixed in formalin for various times and compared sequencing metrics from next- ...

Abstract	Analyzing RNA samples from formalin-fixed paraffin-embedded (FFPE) tissues is essential for precision medicine. We investigated RNA quantity and quality from FFPE tumor tissues fixed in formalin for various times and compared sequencing metrics from next-generation sequencing (NGS). Hepatocellular carcinoma (HCC) tissues were fixed in 10% neutral buffered formalin (1-240 h) and FFPE blocks were prepared. Total RNA was extracted, and the quantity and quality were assessed using the NanoDrop, Qubit and Bioanalyzer. After preparing sequencing libraries, NGS was performed on the Oncomine Dx Multi-CDx system. Total RNA yields of all samples met the threshold required for NGS, but longer fixation times resulted in decreased total RNA and long RNA fragment (>200 nt) yields. NGS analysis showed fewer sequencing reads of internal control genes from RNA with longer fixation times. RNA extracted from FFPE blocks stored for 500 days had reduced RNA yield and quality compared with RNA obtained from FFPE blocks prepared immediately. In conclusion, short and over-fixation should be avoided because of their negative impact on sequencing quality. Fixation process should be finished promptly within recommended guidelines (6-72 h) for cancer patients.
MeSH term(s)	Humans ; Formaldehyde ; Carcinoma, Hepatocellular/genetics ; Tissue Fixation/methods ; RNA ; Paraffin Embedding/methods ; Liver Neoplasms/genetics
Chemical Substances	Formaldehyde (1HG84L3525) ; RNA (63231-63-0)
Language	English
Publishing date	2023-11-07
Publishing country	Australia
Document type	Journal Article
ZDB-ID	1194850-4
ISSN	1440-1827 ; 1320-5463
ISSN (online)	1440-1827
ISSN	1320-5463
DOI	10.1111/pin.13385
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Article ; Online: Liquid biopsy of wash samples obtained via endoscopic ultrasound-guided fine-needle biopsy: Comparison with liquid biopsy of plasma in pancreatic cancer.

Ohyama, Hiroshi / Hirotsu, Yosuke / Amemiya, Kenji / Amano, Hiroyuki / Hirose, Sumio / Oyama, Toshio / Iimuro, Yuji / Kojima, Yuichiro / Mikata, Rintaro / Mochizuki, Hitoshi / Kato, Naoya / Omata, Masao

Diagnostic cytopathology

2024 Volume 52, Issue 6, Page(s) 325–331

Abstract: Objectives: Pancreatic cancer (PC) has a poor prognosis and limited treatment options. Liquid biopsy, which analyzes circulating tumor DNA (ctDNA) in blood, holds promise for precision medicine; however, low ctDNA detection rates pose challenges. This ... ...

Abstract	Objectives: Pancreatic cancer (PC) has a poor prognosis and limited treatment options. Liquid biopsy, which analyzes circulating tumor DNA (ctDNA) in blood, holds promise for precision medicine; however, low ctDNA detection rates pose challenges. This study aimed to investigate the utility of wash samples obtained via endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) as a liquid biopsy for PC. Methods: A total of 166 samples (42 formalin-fixed paraffin-embedded [FFPE] tissues, 80 wash samples, and 44 plasma samples) were collected from 48 patients with PC for genomic analysis. DNA was extracted and quantified, and 60 significantly mutated genes were sequenced. The genomic profiles of FFPE tissues, wash samples, and plasma samples were compared. Finally, the ability to detect druggable mutations in 80 wash samples and 44 plasma samples was investigated. Results: The amount of DNA was significantly lower in plasma samples than in wash samples. Genomic analysis revealed a higher detection rate of oncogenic mutations in FFPE tissues (98%) and wash samples (96%) than in plasma samples (18%) and a comparable detection rate in FFPE tissues and wash samples. Tumor-derived oncogenic mutations were detected more frequently in wash samples than in plasma samples. Furthermore, the oncogenic mutations detection rate remained high in wash samples at all PC stages but low in plasma samples even at advanced PC stages. Using wash samples was more sensitive than plasma samples for identifying oncogenic and druggable mutations. Conclusions: The wash sample obtained via EUS-FNB is an ideal specimen for use as a liquid biopsy for PC.
MeSH term(s)	Humans ; Pancreatic Neoplasms/pathology ; Pancreatic Neoplasms/blood ; Pancreatic Neoplasms/diagnosis ; Pancreatic Neoplasms/genetics ; Endoscopic Ultrasound-Guided Fine Needle Aspiration/methods ; Liquid Biopsy/methods ; Female ; Male ; Aged ; Middle Aged ; Circulating Tumor DNA/blood ; Mutation ; Aged, 80 and over ; Biomarkers, Tumor/blood ; Adult
Chemical Substances	Circulating Tumor DNA ; Biomarkers, Tumor
Language	English
Publishing date	2024-03-22
Publishing country	United States
Document type	Journal Article ; Comparative Study
ZDB-ID	632710-2
ISSN	1097-0339 ; 8755-1039
ISSN (online)	1097-0339
ISSN	8755-1039
DOI	10.1002/dc.25306
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 2117: Show issues

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Article ; Online: Squamous Cell Carcinoma of the Lung With Micropapillary Pattern.

Oyama, Toshio / Goto, Taichiro / Amemiya, Kenji / Hirotsu, Yosuke / Omata, Masao

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

2020 Volume 15, Issue 9, Page(s) 1541–1544

MeSH term(s)	Adenocarcinoma ; Carcinoma, Squamous Cell ; Humans ; Lung ; Lung Neoplasms
Language	English
Publishing date	2020-06-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2432037-7
ISSN	1556-1380 ; 1556-0864
ISSN (online)	1556-1380
ISSN	1556-0864
DOI	10.1016/j.jtho.2020.05.023
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Zs.A 6664: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MO 652: Show issues

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Article: Simple and rapid method to obtain high-quality tumor dna from clinical-pathological specimens using touch imprint cytology

Amemiya, Kenji / Hirotsu, Yosuke / Oyama, Toshio / Omata, Masao

Journal of visualized experiments. 2018 Mar. 21, , no. 133

2018

Abstract: It is critical to determine the mutational status in cancer before administration and treatment of specific molecular targeted drugs for cancer patients. In the clinical setting, formalin-fixed paraffin-embedded (FFPE) tissues are widely used for genetic ...

Abstract	It is critical to determine the mutational status in cancer before administration and treatment of specific molecular targeted drugs for cancer patients. In the clinical setting, formalin-fixed paraffin-embedded (FFPE) tissues are widely used for genetic testing. However, FFPE DNA is generally damaged and fragmented during the fixation process with formalin. Therefore, FFPE DNA is sometimes not adequate for genetic testing because of low quality and quantity of DNA. Here we present a method of touch imprint cytology (TIC) to obtain genomic DNA from cancer cells, which can be observed under a microscope. Cell morphology and cancer cell numbers can be evaluated using TIC specimens. Furthermore, the extraction of genomic DNA from TIC samples can be completed within two days. The total amount and quality of TIC DNA obtained using this method was higher than that of FFPE DNA. This rapid and simple method allows researchers to obtain high-quality DNA for genetic testing (e.g., next generation sequencing analysis, digital PCR, and quantitative real time PCR) and to shorten the turnaround time for reporting results.
Keywords	DNA ; drugs ; formalin ; genetic testing ; high-throughput nucleotide sequencing ; neoplasm cells ; neoplasms ; patients ; quantitative polymerase chain reaction ; rapid methods ; tissues
Language	English
Dates of publication	2018-0321
Size	p. e56943.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/56943
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology.

Amemiya, Kenji / Hirotsu, Yosuke / Oyama, Toshio / Omata, Masao

Journal of visualized experiments : JoVE

2018 , Issue 133

Abstract	It is critical to determine the mutational status in cancer before administration and treatment of specific molecular targeted drugs for cancer patients. In the clinical setting, formalin-fixed paraffin-embedded (FFPE) tissues are widely used for genetic testing. However, FFPE DNA is generally damaged and fragmented during the fixation process with formalin. Therefore, FFPE DNA is sometimes not adequate for genetic testing because of low quality and quantity of DNA. Here we present a method of touch imprint cytology (TIC) to obtain genomic DNA from cancer cells, which can be observed under a microscope. Cell morphology and cancer cell numbers can be evaluated using TIC specimens. Furthermore, the extraction of genomic DNA from TIC samples can be completed within two days. The total amount and quality of TIC DNA obtained using this method was higher than that of FFPE DNA. This rapid and simple method allows researchers to obtain high-quality DNA for genetic testing (e.g., next generation sequencing analysis, digital PCR, and quantitative real time PCR) and to shorten the turnaround time for reporting results.
MeSH term(s)	Cytodiagnosis/methods ; DNA, Neoplasm/genetics ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Sequence Analysis, DNA/methods
Chemical Substances	DNA, Neoplasm
Language	English
Publishing date	2018-03-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/56943
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Surgery for congenital bronchial atresia.

Higuchi, Rumi / Goto, Taichiro / Nakagomi, Takahiro / Oyama, Toshio

Asian cardiovascular & thoracic annals

2018 Volume 26, Issue 6, Page(s) 485–488

Abstract: A 22-year-old woman presented with a feeling of chest tightness. Chest computed tomography showed a dendritic shadow in the left segment 1 + 2c, surrounded by hyperlucent emphysematous changes. Bronchoscopy revealed loss of the orifice of bronchus 1 + 2c. ...

Abstract	A 22-year-old woman presented with a feeling of chest tightness. Chest computed tomography showed a dendritic shadow in the left segment 1 + 2c, surrounded by hyperlucent emphysematous changes. Bronchoscopy revealed loss of the orifice of bronchus 1 + 2c. Thus a diagnosis of bronchial atresia was made, and a left upper division segmentectomy was performed. Bronchial atresia should be considered in the differential diagnosis of young patients with an abnormal chest shadow. It can be diagnosed based on the characteristic imaging and bronchoscopic findings. Surgery is indicated for symptomatic bronchial atresia and can provide complete amelioration of the condition.
MeSH term(s)	Bronchi/abnormalities ; Bronchi/diagnostic imaging ; Bronchi/surgery ; Bronchial Diseases/congenital ; Bronchial Diseases/diagnosis ; Bronchial Diseases/surgery ; Bronchoscopy ; Female ; Humans ; Pneumonectomy/methods ; Tomography, X-Ray Computed ; Young Adult
Language	English
Publishing date	2018-06-03
Publishing country	England
Document type	Case Reports ; Journal Article ; Video-Audio Media
ZDB-ID	1400468-9
ISSN	1816-5370 ; 0218-4923
ISSN (online)	1816-5370
ISSN	0218-4923
DOI	10.1177/0218492318780805
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Zs.A 5193: Show issues

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Article ; Online: Relationship between formalin reagent and success rate of targeted sequencing analysis using formalin fixed paraffin embedded tissues.

Amemiya, Kenji / Hirotsu, Yosuke / Oyama, Toshio / Omata, Masao

Clinica chimica acta; international journal of clinical chemistry

2018 Volume 488, Page(s) 129–134

Abstract: Background: Tumor genetic alterations are determined to aid in selecting therapy and predicting prognosis. In routine clinical practice, targeted sequencing analysis is performed using formalin-fixed paraffin embedded (FFPE) tissues. However, successful ...

Abstract	Background: Tumor genetic alterations are determined to aid in selecting therapy and predicting prognosis. In routine clinical practice, targeted sequencing analysis is performed using formalin-fixed paraffin embedded (FFPE) tissues. However, successful genetic analysis remains challenging because FFPE DNA is fragmented during the sample preparation process. Methods: Real-time PCR was performed to assess DNA quality and quantities. Targeted sequencing was performed using FFPE tissues fixed with different types of formalin. Results: DNA was less fragmented from samples fixed in low formalin concentration (10% vs. 20%) and neutral buffered conditions (neutral buffered vs. non-neutral). DNA fragmentation increased over the fixation time. In a preliminary test study, we compared fixation using 10% neutral buffered formalin (n = 180) and 20% formalin (n = 26). The success rate of targeted analysis was higher using 10% neutral formalin (98.3%; 177/180) compared with 20% formalin (34.6%; 9/26). In a validation study with additional formalin-fixed paraffin embedded tissues fixed with 10% neutral buffered formalin (n = 860), we reproduced these results and achieved a high success rate for targeted sequencing analysis (98.4%; 846/860). Conclusion: Our data show that 10% neutral buffered formalin is recommended for fixation of formalin-fixed paraffin embedded samples to achieve high success rate of targeted sequencing analysis.
MeSH term(s)	DNA, Neoplasm/genetics ; DNA, Neoplasm/isolation & purification ; Formaldehyde/chemistry ; Humans ; Paraffin Embedding ; Real-Time Polymerase Chain Reaction ; Sequence Analysis, DNA ; Tissue Fixation
Chemical Substances	DNA, Neoplasm ; Formaldehyde (1HG84L3525)
Language	English
Publishing date	2018-11-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80228-1
ISSN	1873-3492 ; 0009-8981
ISSN (online)	1873-3492
ISSN	0009-8981
DOI	10.1016/j.cca.2018.11.002
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