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  1. Article ; Online: Neither lateral patellar facet nor patellar size are altered in patellofemoral unstable patients: a comparative magnetic resonance imaging analysis.

    Otto, Alexander / Tscholl, Philippe M / Pääsuke, Reedik / Herbst, Elmar / Willinger, Lukas / Imhoff, Andreas B / Brucker, P U

    Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA

    2019  Volume 28, Issue 4, Page(s) 1064–1071

    Abstract: Purpose: It remains unclear if morphologic patterns of the patella itself predispose to patellar instability. This study examined established patellar landmarks in relation to the femoral condyle width to clarify differences of patellar morphologies in ... ...

    Abstract Purpose: It remains unclear if morphologic patterns of the patella itself predispose to patellar instability. This study examined established patellar landmarks in relation to the femoral condyle width to clarify differences of patellar morphologies in patellofemoral stable and unstable patients.
    Methods: Magnetic Resonance Imaging of 50 subjects (20.7 ± 4.4 years; 17 males, 33 females) with patellofemoral instability (study group, SG) and 50 subjects (25.3 ± 5.8 years; 31 males, 19 females) with anterior cruciate ligament rupture (control group, CG) were analyzed. Corresponding patellar value indices (PW-I; LPF-I 1; LPF-I 2) in relation to the femoral condyle width (FCW) were evaluated after the measurement of absolute patellar dimension [patellar width (PW); direct length of the lateral patellar facet (LPF-1); projected length of the lateral patellar facet (LPF-2)]. The patellar shape according to Wiberg, trochlear dysplasia, patellar height, and tibial tubercle-trochlear groove (TT-TG) distance were determined.
    Results: The SG showed a significantly longer absolute (LPF 2) (P = 0.041) and relative (LPF-I 1, LPF-I 2) (P < 0.001) lateral facet of the patella. No significant differences were evaluable for the relative patellar width (PW-I) (ns). A patellar shape type 3 (P = 0.001) as well as a higher position of the patella and TT-TG-distance (P < 0.001) were significantly more often present in the SG.
    Conclusion: There are several bony alterations associated with patellofemoral instability, but our data did not show a significantly smaller lateral patellar facet or relative patellar width that could facilitate a patellar dislocation. This helps surgeons, that are considering to surgically address the patella in cases of patellofemoral instability, to better understand its morphologic pattern.
    Level of evidence: III.
    MeSH term(s) Adolescent ; Adult ; Anterior Cruciate Ligament Injuries/pathology ; Anterior Cruciate Ligament Injuries/surgery ; Anterior Cruciate Ligament Reconstruction ; Female ; Femur/pathology ; Humans ; Joint Instability/pathology ; Knee Joint/pathology ; Magnetic Resonance Imaging ; Male ; Patella/surgery ; Patellar Dislocation/diagnostic imaging ; Patellar Dislocation/surgery ; Patellofemoral Joint/diagnostic imaging ; Patellofemoral Joint/surgery ; Recurrence ; Tibia/surgery ; Young Adult
    Language English
    Publishing date 2019-08-27
    Publishing country Germany
    Document type Comparative Study ; Journal Article
    ZDB-ID 1159064-6
    ISSN 1433-7347 ; 0942-2056
    ISSN (online) 1433-7347
    ISSN 0942-2056
    DOI 10.1007/s00167-019-05660-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Vitamin C and E Treatment Blunts Sprint Interval Training-Induced Changes in Inflammatory Mediator-, Calcium-, and Mitochondria-Related Signaling in Recreationally Active Elderly Humans.

    Wyckelsma, Victoria L / Venckunas, Tomas / Brazaitis, Marius / Gastaldello, Stefano / Snieckus, Audrius / Eimantas, Nerijus / Baranauskiene, Neringa / Subocius, Andrejus / Skurvydas, Albertas / Pääsuke, Mati / Gapeyeva, Helena / Kaasik, Priit / Pääsuke, Reedik / Jürimäe, Jaak / Graf, Brigitte A / Kayser, Bengt / Place, Nicolas / Andersson, Daniel C / Kamandulis, Sigitas /
    Westerblad, Håkan

    Antioxidants (Basel, Switzerland)

    2020  Volume 9, Issue 9

    Abstract: Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants ...

    Abstract Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants vitamin C and E. Recreationally active elderly (mean age 65) men received either vitamin C (1 g/day) and vitamin E (235 mg/day) or placebo. Training consisted of nine SIT sessions (three sessions/week for three weeks of 4-6 repetitions of 30-s all-out cycling sprints) interposed by 4 min rest. Vastus lateralis muscle biopsies were taken before, 1 h after, and 24 h after the first and last SIT sessions. At the end of the three weeks of training, SIT-induced changes in relative mRNA expression of reactive oxygen/nitrogen species (ROS)- and mitochondria-related proteins, inflammatory mediators, and the sarcoplasmic reticulum Ca
    Language English
    Publishing date 2020-09-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox9090879
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Vitamin C and E Treatment Blunts Sprint Interval Training–Induced Changes in Inflammatory Mediator-, Calcium-, and Mitochondria-Related Signaling in Recreationally Active Elderly Humans

    Wyckelsma, Victoria L / Venckunas, Tomas / Brazaitis, Marius / Gastaldello, Stefano / Snieckus, Audrius / Eimantas, Nerijus / Baranauskiene, Neringa / Subocius, Andrejus / Skurvydas, Albertas / Pääsuke, Mati / Gapeyeva, Helena / Kaasik, Priit / Pääsuke, Reedik / Jürimäe, Jaak / Graf, Brigitte A / Kayser, Bengt / Place, Nicolas / Andersson, Daniel C / Kamandulis, Sigitas /
    Westerblad, Håkan

    Antioxidants. 2020 Sept. 17, v. 9, no. 9

    2020  

    Abstract: Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants ...

    Abstract Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants vitamin C and E. Recreationally active elderly (mean age 65) men received either vitamin C (1 g/day) and vitamin E (235 mg/day) or placebo. Training consisted of nine SIT sessions (three sessions/week for three weeks of 4-6 repetitions of 30-s all-out cycling sprints) interposed by 4 min rest. Vastus lateralis muscle biopsies were taken before, 1 h after, and 24 h after the first and last SIT sessions. At the end of the three weeks of training, SIT-induced changes in relative mRNA expression of reactive oxygen/nitrogen species (ROS)- and mitochondria-related proteins, inflammatory mediators, and the sarcoplasmic reticulum Ca²⁺ channel, the ryanodine receptor 1 (RyR1), were blunted in the vitamin treated group. Western blots frequently showed a major (>50%) decrease in the full-length expression of RyR1 24 h after SIT sessions; in the trained state, vitamin treatment seemed to provide protection against this severe RyR1 modification. Power at exhaustion during an incremental cycling test was increased by ~5% at the end of the training period, whereas maximal oxygen uptake remained unchanged; vitamin treatment did not affect these measures. In conclusion, treatment with the antioxidants vitamin C and E blunts SIT-induced cellular signaling in skeletal muscle of elderly individuals, while the present training regimen was too short or too intense for the changes in signaling to be translated into a clear-cut change in physical performance.
    Keywords Western blotting ; age ; antioxidants ; ascorbic acid ; biopsy ; calcium ; cell communication ; clearcutting ; elderly ; gene expression ; humans ; men ; muscles ; nitrogen ; oxygen ; physical activity ; placebos ; proteins ; sarcoplasmic reticulum ; skeletal muscle ; vitamin E
    Language English
    Dates of publication 2020-0917
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox9090879
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Proliferation of Human Primary Myoblasts Is Associated with Altered Energy Metabolism in Dependence on Ageing In Vivo and In Vitro.

    Pääsuke, Reedik / Eimre, Margus / Piirsoo, Andres / Peet, Nadežda / Laada, Liidia / Kadaja, Lumme / Roosimaa, Mart / Pääsuke, Mati / Märtson, Aare / Seppet, Enn / Paju, Kalju

    Oxidative medicine and cellular longevity

    2016  Volume 2016, Page(s) 8296150

    Abstract: BACKGROUND. Ageing is associated with suppressed regenerative potential of muscle precursor cells due to decrease of satellite cells and suppressive intramuscular milieu on their activation, associated with ageing-related low-grade inflammation. The aim ... ...

    Abstract BACKGROUND. Ageing is associated with suppressed regenerative potential of muscle precursor cells due to decrease of satellite cells and suppressive intramuscular milieu on their activation, associated with ageing-related low-grade inflammation. The aim of the study was to characterize the function of oxidative phosphorylation (OXPHOS), glycolysis, adenylate kinase (AK), and creatine kinase (CK) mediated systems in young and older individuals. MATERIALS AND METHODS. Myoblasts were cultivated from biopsies taken by transcutaneous conchotomy from vastus lateralis muscle in young (20-29 yrs, n = 7) and older (70-79 yrs, n = 7) subjects. Energy metabolism was assessed in passages 2 to 6 by oxygraphy and enzyme analysis. RESULTS. In myoblasts of young and older subjects the rate of OXPHOS decreased during proliferation from passages 2 to 6. The total activities of CK and AK decreased. Myoblasts of passage 2 cultivated from young muscle showed higher rate of OXPHOS and activities of CK and AK compared to myoblasts from older subjects while hexokinase and pyruvate kinase were not affected by ageing. CONCLUSIONS. Proliferation of myoblasts in vitro is associated with downregulation of OXPHOS and energy storage and transfer systems. Ageing in vivo exerts an impact on satellite cells which results in altered metabolic profile in favour of the prevalence of glycolytic pathways over mitochondrial OXPHOS of myoblasts.
    MeSH term(s) Adenylate Kinase/metabolism ; Adult ; Age Factors ; Aged ; Aging ; Animals ; Biopsy ; Cell Proliferation ; Cells, Cultured ; Creatine Kinase/metabolism ; Energy Metabolism ; Glycolysis ; Hexokinase/metabolism ; Humans ; Inflammation ; Muscle, Skeletal/metabolism ; Myoblasts/metabolism ; Oxidative Phosphorylation ; Oxygen/chemistry ; Pyruvate Kinase/metabolism ; Satellite Cells, Skeletal Muscle/cytology ; Young Adult
    Chemical Substances Hexokinase (EC 2.7.1.1) ; Pyruvate Kinase (EC 2.7.1.40) ; Creatine Kinase (EC 2.7.3.2) ; Adenylate Kinase (EC 2.7.4.3) ; Oxygen (S88TT14065)
    Language English
    Publishing date 2016
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1942-0994
    ISSN (online) 1942-0994
    DOI 10.1155/2016/8296150
    Database MEDical Literature Analysis and Retrieval System OnLINE

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