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Article ; Online: Bacteremia caused by

Sidak, Kateryna / Pérez-Tanoira, Ramón / Gomez-Herruz, Peña

Advances in laboratory medicine

2021 Volume 3, Issue 1, Page(s) 91–96

Abstract: Objectives: Helicobacter cinaedi: Case presentation: A 49-year-old patient with ulcerative colitis diagnosed in 1992, who presented to the ED of our hospital with fever and testicular complaints. The patient was discharged with a diagnosis of left- ... ...

Abstract	Objectives: Helicobacter cinaedi Case presentation: A 49-year-old patient with ulcerative colitis diagnosed in 1992, who presented to the ED of our hospital with fever and testicular complaints. The patient was discharged with a diagnosis of left-sided acute epididymitis, which was probably sexually transmitted. At the ED, he was administered intravenous Ceftriaxone and discharged with a prescription of doxycycline for 10 days, with a good progress. Aerobic cultures were positive at three days from collection. Gram staining showed Gram-negative, corkscrew-shaped bacteria. The analysis of the blood culture bottles, and the colonies grown in Campylosel agar incubated in microaerophilic conditions at 42 °C were identified as Conclusions: Direct analysis of the blood culture bottle on the Maldi-TOF system allowed for the identification of the etiology of the bacteremia since
Language	Spanish
Publishing date	2021-12-30
Publishing country	Germany
Document type	Case Reports
ISSN	2628-491X
ISSN (online)	2628-491X
DOI	10.1515/almed-2021-0092
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Article ; Online: Comparison of chemiluminiscence versus lateral flow assay for the detection of Helicobacter pylori antigen in human fecal samples.

Rescalvo-Casas, Carlos / Hernando-Gozalo, Marcos / Pereda, Laura Seijas / Bertolín, Carlos García / Pérez-García, Felipe / Cuadros-González, Juan / Pérez-Tanoira, Ramón

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

2023 Volume 42, Issue 8, Page(s) 959–962

Abstract: Helicobacter pylori is a Gram-negative bacterium that causes chronic gastric inflammation, which can lead to gastric neoplasia. Therefore, early diagnosis of H. pylori infection is crucial for effective treatment and prevention of complications. The aim ... ...

Abstract	Helicobacter pylori is a Gram-negative bacterium that causes chronic gastric inflammation, which can lead to gastric neoplasia. Therefore, early diagnosis of H. pylori infection is crucial for effective treatment and prevention of complications. The aim of this study was to compare the sensitivity and specificity of the STANDARD™ F H. pylori Ag FIA stool antigen test (SD Biosensor) with the LIAISON® Meridian H. pylori SA for the diagnosis of H. pylori infection. A total of 133 stool samples from patients with suspected H. pylori infection were compared using the STANDARD™ F H. pylori Ag FIA stool antigen test (SD Biosensor), based on lateral flow assay, with the LIAISON® Meridian H. pylori SA. Of the 45 positive samples with LIAISON, 44 were also positive while 1 was negative in the STANDARD™ antigen test. However, this discrepant sample showed a chemiluminescence index of 1.18, very close to the cut-off point of 1. On the other hand, of 88 negative samples obtained with LIAISON, 83 were negative and 5 were positive in the STANDARD™ antigen test. Moreover, STANDARD™ F H. pylori Ag FIA assay has shown a sensitivity of 97.8% (95% CI: 88.2-99.9), a specificity of 94.3% (95% CI: 87.2-98.1), a PPV of 83.9% (95% CI: 68.9-92.4) and a NPV of 99.3% ((95% CI: 95.3-99.9). In conclusion, the STANDARD™ F H. pylori Ag FIA (SD Biosensor) on the STANDARD™ F2400 analyser is a highly sensitive, specific and suitable assay for the detection of H. pylori in stool samples.
MeSH term(s)	Humans ; Helicobacter pylori ; Sensitivity and Specificity ; Helicobacter Infections/microbiology ; Feces/microbiology ; Antigens, Bacterial
Chemical Substances	Antigens, Bacterial
Language	English
Publishing date	2023-05-27
Publishing country	Germany
Document type	Journal Article
ZDB-ID	603155-9
ISSN	1435-4373 ; 0934-9723 ; 0722-2211
ISSN (online)	1435-4373
ISSN	0934-9723 ; 0722-2211
DOI	10.1007/s10096-023-04624-7
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Article ; Online: Pellagra linked to maize consumption in Gambo, Ethiopia: A call for awareness.

Fernandez-Gonzalez, Pablo / Seijas-Pereda, Laura / Tefasmariam, Abraham / Balcha, Seble / Fernandez-Zarzoso, Miguel / Onoda, Mamiko / Quiles-Recuenco, Ana / Pérez-Tanoira, Ramón / Malmierca, Eduardo

Journal of the European Academy of Dermatology and Venereology : JEADV

2024

Language	English
Publishing date	2024-02-09
Publishing country	England
Document type	Letter
ZDB-ID	1128828-0
ISSN	1468-3083 ; 0926-9959
ISSN (online)	1468-3083
ISSN	0926-9959
DOI	10.1111/jdv.19829
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Article ; Online: Perspectives of primary care physicians in Spain on malaria: a cross-sectional survey and retrospective review of cases.

Linares-Rufo, Manuel / Bermudez-Marval, Harold / García-Bertolín, Carlos / Santos-Galilea, Joaquín / Balsa-Vázquez, Javier / Pérez-Tanoira, Ramón / Santos-Larrégola, Laura / Cuadros-González, Juan / Rojo-Marcos, Gerardo / Ramos-Rincón, José-Manuel

Malaria journal

2024 Volume 23, Issue 1, Page(s) 7

Abstract: Background: In Spain, the risk of imported malaria has increased in recent years due to the rise in international travel and migration. Little is known about the knowledge, information sources, clinical practice, and specific needs of primary care ... ...

Abstract	Background: In Spain, the risk of imported malaria has increased in recent years due to the rise in international travel and migration. Little is known about the knowledge, information sources, clinical practice, and specific needs of primary care physicians (PCPs) concerning malaria despite the pivotal role played by these professionals in managing the health of tourists. The objective of this study was to assess the knowledge, attitudes, and practices of PCPs in Spain regarding malaria. Methods: This research analyses data from (1) a cross-sectional nationwide survey assessing the knowledge and attitudes of PCPs regarding malaria, and (2) a retrospective review of 373 malaria cases appearing in primary care medical records (PCMRs) in the Madrid area over the past 15 years to determine how cases were documented, managed, or characterized in the primary care setting. Results: The survey findings reveal a modest level of self-perceived familiarity with malaria (221/360, 57.6%), even though 32.8% of the practitioners reported having delivered care for confirmed or suspected cases of the disease, these practitioners had greater knowledge of malaria (80.4%) compared to physicians who reported not having delivered care for malaria (19.6%, p < 0.001). Ten percent of the survey participants did not know the name of the mosquito that transmits malaria, and only 40.7% would promptly request malaria testing for a traveller with symptoms after a trip to an endemic area. Responses provided by younger PCPs varied to a greater extent than those of their more experienced colleagues regarding prevention practices and patient management. A review of PCMRs showed that only 65% of all patients were recorded as such. Among those registered, only 40.3% had a documented malaria episode, and of those, only 16.6% received proper follow-up. Only 23.7% of the patients with a PCMR had a record that specifically indicated travel to an endemic country or travel classified as visiting friends and relatives (VFR). Conclusions: The findings of this study underscore the critical role of PCPs in the field of travel medicine, particularly given the increase in imported malaria cases. These results highlight the need for targeted training in travel medicine and the need to ensure optimal patient education in care settings.
MeSH term(s)	Humans ; Antimalarials/therapeutic use ; Cross-Sectional Studies ; Malaria/epidemiology ; Physicians, Primary Care ; Retrospective Studies ; Spain/epidemiology ; Travel
Chemical Substances	Antimalarials
Language	English
Publishing date	2024-01-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2091229-8
ISSN	1475-2875 ; 1475-2875
ISSN (online)	1475-2875
ISSN	1475-2875
DOI	10.1186/s12936-023-04826-6
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Article ; Online: Colonic anisakiasis, an infrequent case molecularly characterized by PCR-RFLP.

González-Bertolín, Belén / Hernanz-Ruiz, Nerea / Pérez-Tanoira, Ramón / Perteguer-Prieto, María Jesús

Enfermedades infecciosas y microbiologia clinica (English ed.)

2021 Volume 39, Issue 6, Page(s) 308–309

MeSH term(s)	Anisakiasis/diagnosis ; Chromosome Mapping ; Colon ; Humans ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length
Language	English
Publishing date	2021-05-03
Document type	Case Reports ; Letter
ISSN	2529-993X
ISSN (online)	2529-993X
DOI	10.1016/j.eimce.2021.04.001
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Article: Urinary Tract Infection and Antimicrobial Susceptibility of Bacterial Isolates in Saint Joseph Kitgum Hospital, Kitgum, Uganda.

Carrasco Calzada, Félix / Aguilera-Correa, John Jairo / Cuadros González, Juan / Esteban Moreno, Jaime / Roca Biosca, David / Pérez-Tanoira, Ramón

Antibiotics (Basel, Switzerland)

2022 Volume 11, Issue 4

Abstract: A cross-sectional study of microorganisms isolated from mid-stream urine samples obtained from 139 patients with suspected urinary tract infection (UTI) who presented leukocyturia was conducted from April to June 2019 at Saint Joseph Kitgum Hospital ( ... ...

Abstract	A cross-sectional study of microorganisms isolated from mid-stream urine samples obtained from 139 patients with suspected urinary tract infection (UTI) who presented leukocyturia was conducted from April to June 2019 at Saint Joseph Kitgum Hospital (Uganda). All microorganisms were identified by MALDI-TOF mass spectrometry in a laboratory in Spain. Antimicrobial susceptibility was determined on site using the disc diffusion method (Kirby-Bauer test) and these results were subsequently compared with those obtained in Spain using the Becton Dickinson Phoenix M50 device. The overall prevalence of UTI with bacterial growth was 64.0% (
Language	English
Publishing date	2022-04-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2681345-2
ISSN	2079-6382
ISSN	2079-6382
DOI	10.3390/antibiotics11040504
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Article ; Online: Increased Prevalence of Symptomatic Human Intestinal Spirochetosis in MSM with High-Risk Sexual Behavior in a Cohort of 165 Individuals.

Pérez-Tanoira, Ramón / Tamarit, Marta Del Palacio / Montaña, Ana María Vicente / Carmena, David / Köster, Pamela / Górgolas, Miguel / Fortes Alen, José R / Cabello-Úbeda, Alfonso / Prieto-Pérez, Laura

Tropical medicine and infectious disease

2023 Volume 8, Issue 5

Abstract: Human intestinal spirochetosis (HIS) can cause gastrointestinal symptoms, although asymptomatic infections have been described. Individuals from low-income countries, people living with HIV, and men who have sex with men (MSM) show increased risk. A ... ...

Abstract	Human intestinal spirochetosis (HIS) can cause gastrointestinal symptoms, although asymptomatic infections have been described. Individuals from low-income countries, people living with HIV, and men who have sex with men (MSM) show increased risk. A retrospective review of all patients diagnosed with HIS (n = 165) between January 2013 and October 2020 at a tertiary hospital in Madrid, Spain, was performed to assess risk factors for symptomatic HIS, symptoms, and response to treatment. Most patients were male (n = 156; 94.5%), 86.7% were MSM, and 23.5% practiced chemsex, of whom most were symptomatic (
Language	English
Publishing date	2023-04-26
Publishing country	Switzerland
Document type	Journal Article
ISSN	2414-6366
ISSN (online)	2414-6366
DOI	10.3390/tropicalmed8050250
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Article ; Online: Enterocytozoon bieneusi and Encephalitozoon intestinalis (microsporidia) in HIV-positive patients in central Spain.

Chozas, Mercedes / Dashti, Alejandro / Prieto-Pérez, Laura / Pérez-Tanoira, Ramón / Cobo, Elena / Bailo, Begoña / Del Palacio, Marta / Hernández-Castro, Carolina / González-Barrio, David / Carmena, David / Köster, Pamela C

Medical mycology

2023 Volume 61, Issue 4

Abstract: Microsporidia are fungi-related eukaryotic intracellular parasites that opportunistically infect immunocompromised individuals such as those infected by the human immunodeficiency virus (HIV). Among them, Enterocytozoon bieneusi and Encephalitozoon spp. ... ...

Abstract	Microsporidia are fungi-related eukaryotic intracellular parasites that opportunistically infect immunocompromised individuals such as those infected by the human immunodeficiency virus (HIV). Among them, Enterocytozoon bieneusi and Encephalitozoon spp. are the most clinically relevant species. We investigated the occurrence and genetic diversity of microsporidial and protist infections in mostly immunocompetent HIV-positive patients in Madrid, Spain. A structured questionnaire was used to retrieve data on factors potentially associated with an increased risk of infection, including sexual attitudes and sex-risk behaviour. Faecal samples (n = 96) from 81 HIV-positive patients were collected and analysed by molecular (PCR and Sanger sequencing) methods. Two microsporidial pathogens were detected: Ent. bieneusi (2.5%, 95% CI: 0.3-8.6) and Enc.intestinalis (4.9%, 95% CI: 1.4-12.2). The two Ent. bieneusi isolates were identified as zoonotic genotype A. Among protists, Entamoeba dispar was the species most prevalently found (33.3%, 95% CI: 23.2-44.7), followed by Blastocystis spp. (19.8%, 95% CI: 11.7-30.1), Giardia duodenalis (13.6%, 95% CI: 7.0-23.0), and Cryptosporidium spp. and Entamoeba histolytica (2.5%, 95% CI: 0.3-8.6 each). Cyclospora cayetanensis and Cystoisospora belli were not detected. Subtypes ST1 (70.6%, 12/17) and ST3 (29.4%, 5/17) were identified within Blastocystis sp., sub-assemblages AII and BIII (50%, 1/2 each) within G. duodenalis, and Cry. parvum and canine-adapted Cry. canis (50%, 1/2 each) within Cryptosporidium spp. Microsporidial and protist parasites were frequent in well-controlled, mostly immunocompetent HIV-positive patients and should be included in diagnostic algorithms when diarrhoea is present.
MeSH term(s)	Animals ; Dogs ; Humans ; Cryptosporidiosis/complications ; Cryptosporidiosis/epidemiology ; Cryptosporidiosis/parasitology ; Cryptosporidium/genetics ; Encephalitozoon/genetics ; Enterocytozoon/genetics ; Feces ; Genotype ; HIV Infections/complications ; HIV Infections/epidemiology ; HIV Infections/veterinary ; Microsporidia/genetics ; Prevalence ; Spain/epidemiology ; Protozoan Infections/complications ; Protozoan Infections/epidemiology ; Protozoan Infections/parasitology ; Microsporidiosis/complications ; Microsporidiosis/epidemiology ; Microsporidiosis/microbiology
Language	English
Publishing date	2023-04-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1421796-x
ISSN	1460-2709 ; 1369-3786
ISSN (online)	1460-2709
ISSN	1369-3786
DOI	10.1093/mmy/myad039
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Zs.A 520: Show issues

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Article ; Online: Reduction of methicillin-resistant

Aguilera-Correa, John Jairo / Nohynek, Liisa / Alakomi, Hanna-Leena / Esteban, Jaime / Oksman-Caldentey, Kirsi-Marja / Puupponen-Pimiä, Riitta / Kinnari, Teemu J / Perez-Tanoira, Ramon

Frontiers in cellular and infection microbiology

2023 Volume 13, Page(s) 1176755

Abstract: Introduction: Surgical site infection remains a devastating and feared complication of surgery caused mainly by : Methods: This study aimed to evaluate the effect of various extracts from two arctic berries, cloudberry (: Results: All berry ... ...

Abstract	Introduction: Surgical site infection remains a devastating and feared complication of surgery caused mainly by Methods: This study aimed to evaluate the effect of various extracts from two arctic berries, cloudberry ( Results: All berry extracts prevented biofilm development of the three MRSA strains, except the raspberry press cake hydrothermal extract, which produced a diminished anti-staphylococcal effect. Discussion: The studied arctic berry extracts can be used as a treatment for a mature MRSA biofilm, however some limitations in their use exist.
MeSH term(s)	Humans ; Methicillin-Resistant Staphylococcus aureus ; Staphylococcus aureus ; Fruit ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Staphylococcal Infections/microbiology ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Biofilms ; Growth and Development ; Microbial Sensitivity Tests
Chemical Substances	Anti-Bacterial Agents ; Plant Extracts
Language	English
Publishing date	2023-06-23
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2023.1176755
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Article ; Online: Differences in Virulence Factors and Antimicrobial Susceptibility of Uropathogenic

Carrasco Calzada, Félix / Jairo Aguilera, John / Moreno, Jaime Esteban / Cuadros González, Juan / Roca Biosca, David / Prieto-Pérez, Laura / Pérez-Tanoira, Ramón

Tropical medicine and infectious disease

2023 Volume 8, Issue 5

Abstract: Enterococcus ... ...

Abstract	Enterococcus faecalis
Language	English
Publishing date	2023-05-16
Publishing country	Switzerland
Document type	Journal Article
ISSN	2414-6366
ISSN (online)	2414-6366
DOI	10.3390/tropicalmed8050282
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