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Article ; Online: Effect of foot and mouth disease vaccination on seminal antioxidant profiles of mithun (Bos frontalis)

P Perumal

Asian Pacific Journal of Reproduction, Vol 7, Iss 4, Pp 178-

2018 Volume 184

Abstract: Objective: To assess the deleterious effects of foot and mouth disease vaccination on antioxidant profiles as well as oxidative stress in the semen of breeding mithun bulls. Methods: A total of 160 semen samples were collected from 8 adult healthy mithun ...

Abstract	Objective: To assess the deleterious effects of foot and mouth disease vaccination on antioxidant profiles as well as oxidative stress in the semen of breeding mithun bulls. Methods: A total of 160 semen samples were collected from 8 adult healthy mithun bulls with good body condition score (5-6), which were maintained at semen collection centre, ICAR-National Research Centre on Mithun, Medziphema, Nagaland, India, twice a week, 4 weeks before vaccination (pre-vaccination stage) and 12 weeks after vaccination (post-vaccination stage) to understand and know the influence of vaccine stress on seminal antioxidant as well as oxidative stress profiles in mithun. Vaccine was injected at the end of week 4 and semen samples were collected & analysed up to week 16 of the investigation period. Results: Data analysis showed that foot and mouth disease vaccination has significantly (P<0.05) altered the seminal antioxidant, biochemical and oxidative stress profiles up to week 10th of vaccine administration. However, the immunised bulls were recovered in physical health status, semen production and its antioxidant profiles gradually. Conclusions: The results of the present study suggest that the semen sample collection as well as preservation should to be suspended up to week 10th of post immunization to get antioxidant and biochemical profiles in normal level in the semen to preserve normal conception rate in artificial breeding programme by using such semen in mithun species.
Keywords	Antioxidants profiles ; Foot and mouth disease vaccination ; Mithun ; Oxidative profile ; Medicine ; R
Subject code	630
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Wolters Kluwer Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Nebulizer spray pyrolysis Sm3+ doped TiO2 thin film characteristics and room temperature gas sensing

B. Revathi / P. Perumal / E. Arulkumar / S. Thanikaikarasan / R. Kanimozhi / M. Saravannan

Results in Chemistry, Vol 7, Iss , Pp 101254- (2024)

1481

Abstract: In this work, pure TiO2 and Samarium doped (1, 3, 5 wt%) TiO2 thin films was successfully deposited on the FTO substrate by Nebulizer spray pyrolysis technique. The result suggested prepared TiO2 film have tetragonal structure and an anatase phase along ... ...

Abstract	In this work, pure TiO2 and Samarium doped (1, 3, 5 wt%) TiO2 thin films was successfully deposited on the FTO substrate by Nebulizer spray pyrolysis technique. The result suggested prepared TiO2 film have tetragonal structure and an anatase phase along the (101) direction. According to the UV–Vis-NIR spectroscopic analysis, all the deposited films exhibited a high optical percentage of transmittance, surpassing 80 % in the visible region. The FESEM observation indicated that the TiO2 surface morphology is highly sensitive to the increasing of dopant concentration. The FTIR spectroscopic results confirmed the presence of functional chemical bonding in the deposited films, as evidenced by the reflectance spectra. The ethanol sensing properties of 3 % Sm doped TiO2 films found to exhibit rapid response time at 32 s and a recovery at 20 s for 200 ppm at room temperature for 200 ppm concentration. Moreover, 3 % Sm doped TiO2 thin film electrical resistance, which was measured at 0.6 × 106 O, decreased significantly compared to other materials, indicating enhanced gas sensing properties.
Keywords	Bandgap ; Optical properties ; Spray pyrolysis ; Sensors ; TiO2 ; Chemistry ; QD1-999
Subject code	620
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands

P Perumal / Jai Sunder / A K De / D Bhattacharya / A K Nahak / R Vikram / E B Chakurkar

Asian Pacific Journal of Reproduction, Vol 12, Iss 6, Pp 288-

2023 Volume 298

Abstract: Objective: To measure seasonal effects on semen quality profiles, seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons. Methods: A total of 10 Teressa bucks (3-4 years) were selected from ... ...

Abstract	Objective: To measure seasonal effects on semen quality profiles, seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons. Methods: A total of 10 Teressa bucks (3-4 years) were selected from breeding farm, ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands, India. Semen samples (n=25 per season) were collected through artificial vagina method and preserved at refrigerated temperature (5°C) for 48 h using Tris citrate glucose based extender. We detected semen quality parameters [volume, mass activity, pH, sperm concentration, total motility, viability, total sperm abnormality, and plasma membrane, acrosomal and nuclear integrities], biochemical profiles [aspartate amino transferase (AST), alanine amino transferase (ALT) and total cholesterol], and oxidative stress markers [total antioxidant capacity (TAC) and malondialdehyde (MDA)] during monsoon and dry seasons. Results: Semen quality parameters significantly differed between seasons (P<0.05) and among storage periods (P<0.05). Volume, pH, mass activity, motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly higher (P<0.05). Sperm concentration, sperm abnormalities, MDA, AST, ALT and total cholesterol were significantly lower in fresh semen of monsoon than dry season (P<0.05). Motility, viability, acrosomal, plasma membrane and nuclear integrities, and TAC were significantly decreased (P<0.05) while sperm abnormality, AST, ALT, total cholesterol and MDA were significantly increased as liquid semen storage period advanced (P<0.05). Conclusions: Monsoon season has higher beneficial effects on semen quality profiles and liquid stored semen remained usable for upto 48 h. Good quality ejaculates with higher TAC and lower MDA can be cryopreserved and will be used for artificial insemination.
Keywords	andaman and nicobar islands ; teressa goat ; semen quality profiles ; season ; liquid storage ; Medicine ; R
Subject code	630
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Wolters Kluwer Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Climate smart crop-livestock integrated farming as a sustainable agricultural strategy for humid tropical islands

T. P. Swarnam / A. Velmurugan / T. Subramani / N. Ravisankar / N. Subash / A. S. Pawar / P. Perumal / I. Jaisankar / S. Dam Roy

International Journal of Agricultural Sustainability, Vol 22, Iss

2024 Volume 1

Abstract: ABSTRACTClimate change and its impacts on agriculture are relatively well documented at the global and regional levels, while adaptation measures to sustain food production are mostly location specific. Such measures integrated into a production system ... ...

Abstract	ABSTRACTClimate change and its impacts on agriculture are relatively well documented at the global and regional levels, while adaptation measures to sustain food production are mostly location specific. Such measures integrated into a production system as a farming practice will become robust based on its performance at smallholder farms. Therefore, a long-term (2011-2018) study was undertaken to evaluate these practices in a crop-livestock integrated farming system (IFS) approach as a climate-smart agricultural (CSA) strategy for enhancing agricultural production on a humid tropical island. The results showed a significant increase in on-farm productivity from 2.8 t ha−1 in 2011–35.6 t ha−1 in 2018 and net returns from USD 359 to more than 3500 ha−1 due to the adoption of CSA practices. Most importantly the study found increase in the sustainability index (0.89) by the adoption of CSA practices such as crop and enterprise diversification and land manipulation. The climate smartness of these practices was witnessed in reduced greenhouse gas (GHG) emissions and expanded carbon sink that resulted in a mitigation benefit of 5.40 Mg CO2eq ha−1yr−1. CSA practices such as crop diversification through raised beds, enterprise diversification, crop rotation, green manuring, in-situ rainwater harvesting, and contingency cropping significantly contributed to enhancing the production of crop-livestock IFS. Crop residue mulching, agro-forestry and organic waste recycling contributed for enlarging the C sink and minimizing net GHG emissions. Based on these findings, we demonstrated that a crop-livestock IFS was the best climate-smart strategy to enhance the productivity and food security of smallholder farmers in a sustainable way against the consequences of climate change and to increase the mitigating potential through carbon stocking.
Keywords	Climate change ; smallholder farming ; diversification ; adaptation and resilience ; sustainability ; Agriculture ; S
Subject code	333
Language	English
Publishing date	2024-12-01T00:00:00Z
Publisher	Taylor & Francis Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Platyphylloside, a potential inhibitor from epicarp of B. aegyptiaca against CYP450 protein in T. rubrum – In vitro and in silico approaches

Mohamed Hussain Syed Abuthakir / Munirah Abdullah Al-Dosary / Ashraf Atef Hatamleh / Hissah Abdulrahman Alodaini / P. Perumal / Muthusamy Jeyam

Saudi Journal of Biological Sciences, Vol 29, Iss 5, Pp 3899-

2022 Volume 3910

Abstract: Trichophyton rubrum is one of the major disease causing pathogens in human; mainly it causes tinea pedis, tinea cruris and tinea corporis. Cytochrome P450 which considered to be an important protein that can impact ergosterol biosynthesis pathway. B. ... ...

Abstract	Trichophyton rubrum is one of the major disease causing pathogens in human; mainly it causes tinea pedis, tinea cruris and tinea corporis. Cytochrome P450 which considered to be an important protein that can impact ergosterol biosynthesis pathway. B. aegyptiaca is rich source of secondary metabolites with tremendous medicinal values and it has sweet pulp, leaves with spine, strong seed and oily kernel. The epicarp of the fruit was taken for this study to inhibit T. rubrum using in vitro and in silico techniques. The epicarp portion was extracted using various solvents and water. The anti-dermatophytic activity on T. rubrum of these extracts was assessed utilizing poison plate technique with 5 individual concentrations. The fractioned chloroform extract of epicarp had fully inhibited the growth of T. rubrum at 3 mg/ml. Further, the chloroform extract was subjected to LC-MS analysis, in total, 40 compounds were elucidated. Then, the derived compounds were included for predicting ADMETox properties using Qikprop module. From the analysis 40 compounds were identified to be eligible for docking process. Then the desirable compounds, drug Ketoconazole were subjected to docking analysis using Glide module of Schrödinger. It shows that Platyphylloside has better docking result than other compounds and drug Ketoconazole. Further, MD simulation was carried out for Ketoconazole-Cyp450 and Platyphylloside-CYP450 complexes using Desmond, Schrödinger. MD simulation study also confirmed that the Platyphylloside-CYP450 complex more stable. This study suggests that Platyphylloside may act as potential inhibitor and it could be further subjected to experimental analysis to inhibit the T. rubrum growth.
Keywords	Balanites aegyptiaca ; Trichophyton rubrum ; CYP450 ; Platyphylloside ; MD simulation ; Biology (General) ; QH301-705.5
Subject code	540
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of seasons on semen production, effect of melatonin on the liquid storage (5 °C) with correlated study of birth rate in mithun (Bos frontalis)

P Perumal / JK Chamuah / AK Nahak / C Rajkhowa

Asian Pacific Journal of Reproduction, Vol 4, Iss 1, Pp 1-

2015 Volume 12

Abstract: Objective: To assess the effect of melatonin (MT) at different seasons of the year on sperm motility, viability, total sperm abnormality, acrosomal membrane, plasma membrane and nuclear integrity, antioxidant profiles such as superoxide dismutase (SOD), ... ...

Abstract	Objective: To assess the effect of melatonin (MT) at different seasons of the year on sperm motility, viability, total sperm abnormality, acrosomal membrane, plasma membrane and nuclear integrity, antioxidant profiles such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and total antioxidant capacity (TAC), enzymatic profiles such as aspartate amino transaminase (AST), alanine amino transaminase (ALT) and biochemical profiles such as malondialdehyde (MDA) production. Methods: Total numbers of 80 ejaculates (20 ejaculates in each season) were collected twice a week from mithun bulls and semen was split into five equal aliquots, diluted with the tris egg yolk citrate (TEYC) extender. Group 1: semen without additives (control), group 2 to group 5: semen was diluted with 1 mM, 2 mM, 3 mM, and 4 mM of melatonin, respectively. These seminal parameters, antioxidant, enzymatic and biochemical profiles were assessed at liquid storage of mithun semen (5 °C). Simultaneously, retrospective study was conducted on birth rate of calf at different seasons from 2002 to 2012 from farm birth register of mithun farm. Results: Inclusion of melatonin into diluent resulted in significant (P<0.05) decrease in percentages of dead spermatozoa, abnormal spermatozoa and acrosomal abnormalities at different seasons of the year as compared with control group. Additionally, spring season has highest seminal and antioxidant profiles followed by autumn and winter season, whereas lower values were observed in ejaculates collected from summer season. Similarly retrospective study revealed that highest birth rate was in winter followed by autumn, spring and summer season and breeding was occurred in spring, winter, summer and autumn season, respectively with gestation period of 270–290 days. Conclusions: The result of present study indicates that the melatonin protects seminal and antioxidant profiles varied in different seasons, semen quality also varied from different seasons and was highest in spring and lowest in summer ...
Keywords	melatonin (MT) ; Mithun ; Seminal parameters ; Antioxidant profiles ; Biochemical parameters ; Breeding seasons ; Calf birth rate ; Medicine ; R
Subject code	630
Language	English
Publishing date	2015-03-01T00:00:00Z
Publisher	Wolters Kluwer Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Addition of Melatonin on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen

P. Perumal / Kezhavituo Vupru / K. Khate

International Journal of Zoology, Vol

2013 Volume 2013

Abstract: The present study was undertaken to assess the effect of melatonin (MT) on sperm motility, viability, total sperm abnormality, acrosomal and plasma membrane integrity, DNA abnormality, antioxidant profiles such as superoxide dismutase (SOD), catalase ( ... ...

Abstract	The present study was undertaken to assess the effect of melatonin (MT) on sperm motility, viability, total sperm abnormality, acrosomal and plasma membrane integrity, DNA abnormality, antioxidant profiles such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and total antioxidant capacity (TAC), enzymatic profiles such as aspartate amino transaminase (AST), alanine amino transaminase (ALT), and biochemical profiles such as malonaldehyde (MDA) production and cholesterol efflux. Total numbers of 30 ejaculates were collected twice a week from eight mithun bulls and semen was split into five equal aliquots, diluted with the TEYC extender. Group 1 has semen without additives (control) and group 2 to group 5 have semen that was diluted with 1 mM, 2 mM, 3 mM, and 4 mM of melatonin, respectively. These seminal parameters, antioxidant, enzymatic, and biochemical profiles were assessed at 5°C for 0, 6, 12, 24, and 30 h of incubation. Inclusion of melatonin into diluent resulted in significant (P<0.05) decrease in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities at different hours of storage periods as compared with control group. Additionally, melatonin at 3 mM has significant improvement in quality of mithun semen than melatonin at 1 mM, 2 mM or 4 mM stored in in vitro for up to 30 h. It was concluded that the possible protective effects of melatonin on sperm parameters are it prevents MDA production and preserve the antioxidants and intracellular enzymes during preservation.
Keywords	Zoology ; QL1-991
Subject code	630
Language	English
Publishing date	2013-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Addition of Taurine on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen

P. Perumal / Kezhavituo Vupru / C. Rajkhowa

Veterinary Medicine International, Vol

2013 Volume 2013

Abstract: The present study was undertaken to assess the effect of taurine on sperm motility, viability, total sperm abnormalities, acrosomal and plasma membrane integrity, enzymatic profiles such as reduced glutathione (GSH), glutathione peroxidase (GPX), ... ...

Abstract	The present study was undertaken to assess the effect of taurine on sperm motility, viability, total sperm abnormalities, acrosomal and plasma membrane integrity, enzymatic profiles such as reduced glutathione (GSH), glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT), and biochemical profiles such as cholesterol efflux and malondialdehyde (MDA) production. A total of 50 ejaculates were collected twice a week from 8 mithun bulls, and semen was split into 4 equal aliquots and diluted with the TEYC extender. Group 1: semen was without additives (control); groups 2 to 4: semen was diluted with 25 mM, 50 mM, and 100 mM of taurine, respectively. Seminal parameters and enzymatic and biochemical profiles were assessed at 5°C. Inclusion of taurine into diluent resulted in significant () decreases in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities after liquid storage compared with the control group. Additionally, taurine at 50 mM has significant improvement in quality of mithun semen than taurine at 25 or 100 mM stored in in vitro at 5°C. It was concluded that the possible protective effects of taurine on sperm parameters are from enhancing the function of antioxidant enzymes, preventing efflux of cholesterol from cell membranes and decreased MDA production.
Keywords	Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2013-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Phytochemical profiling of marine red alga, Halymenia palmata and its bio-control effects against Dengue Vector, Aedes aegypti

Deepak, P / B. Balamuralikrishnan / S. Park / R. Sowmiya / G. Balasubramani / D. Aiswarya / V. Amutha / P. Perumal

South African Association of Botanists South African journal of botany. 2019 Mar., v. 121

2019

Abstract: The present study undertakes the evaluation of mosquito-larvicidal activity of phytochemical extracts of Halymenia palmata. The larvicidal activity of methanolic crude extract of seaweed, H. palmata (HPMe) and its fractions (Hpf-1 and Hpf-2) fractioned ... ...

Abstract	The present study undertakes the evaluation of mosquito-larvicidal activity of phytochemical extracts of Halymenia palmata. The larvicidal activity of methanolic crude extract of seaweed, H. palmata (HPMe) and its fractions (Hpf-1 and Hpf-2) fractioned by chromatographic technique were assessed against the mosquito larvae of Aedes aegypti. The maximum lethality was recorded by Hpf-2 with LC50 & LC90 values (μg/mL) 23.69 & 233.49 respectively, whereas, HPMe and Hpf-1 showed a moderate activity of: 42.73 and 95.48; 91.95 and 709.04 respectively. The GC–MS analysis of Hpf-2 revealed the presence of 20 bioactive compounds with two major peaks corresponding to Phytol and 2-propamine which may be responsible for mosquito-toxicity. The Hpf-2 treated mosquito larvae (A. aegypti) have shown the progressive deformities in the lateral hair. These findings provide an important baseline data on the mosquito-larvicidal activity of H. palmata although further studies are warranted.
Keywords	Aedes aegypti ; Halymenia ; bioactive compounds ; chemical constituents of plants ; death ; dengue ; gas chromatography-mass spectrometry ; insect larvae ; larvicides ; lethal concentration 50 ; macroalgae
Language	English
Dates of publication	2019-03
Size	p. 257-266.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2126918-X
ISSN	0254-6299
ISSN	0254-6299
DOI	10.1016/j.sajb.2018.11.011
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Antioxidant activities of Indigofera cassioides Rottl. Ex. DC. using various in vitro assay models

R Senthil Kumar / B Rajkapoor / P Perumal

Asian Pacific Journal of Tropical Biomedicine, Vol 2, Iss 4, Pp 256-

2012 Volume 261

Abstract: Objective: To evaluate the antioxidant potential of methanolic leaf extract of Indigofera cassioides (MEIC) using various in vitro antioxidant assay systems. Methods: Antioxidant and free radical scavenging activity of MEIC was assayed by using different ...

Abstract	Objective: To evaluate the antioxidant potential of methanolic leaf extract of Indigofera cassioides (MEIC) using various in vitro antioxidant assay systems. Methods: Antioxidant and free radical scavenging activity of MEIC was assayed by using different in vitro models like ABTS, DPPH, nitric oxide, superoxide, hydrogen peroxide and hydroxyl radical. Reductive ability of the extract was tested by the complex formation with potassium ferricyanide. Further total phenol and flavonoid contents of the crude extract were also determined. Rutin and ascorbic acid were used as standards. Results: MEIC exhibited potent and concentration dependent free radical scavenging activity in all the tested models. Reductive ability was also found to increase with increase in MEIC concentration. Total phenol and flavonoid content determination showed that the extract is rich in phenols and flavonoids. Conclusions: All the results of the in vitro antioxidant assays reveal potent antioxidant and free radical scavenging activity of the leaves of Indigofera cassioides, equivalent to that of standard ascorbic acid and rutin. This potent antioxidant activity may be attributed to its high phenolic and flavonoid contents.
Keywords	Indigofera cassioides ; Antioxidant ; Free radicals ; Oxidative stress ; Total reducing power ; Free radical scavenging activity ; Arctic medicine. Tropical medicine ; RC955-962 ; Biology (General) ; QH301-705.5
Subject code	500
Language	English
Publishing date	2012-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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