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  1. Article: Dynamics of Crimean-Congo hemorrhagic fever virus in two wild ungulate hosts during a disease-induced population collapse.

    Carrera-Faja, Laura / Espunyes, Johan / Cardells, Jesús / Fernández Aguilar, Xavier / Pailler-García, Lola / Napp, Sebastian / Cabezón, Oscar

    One health (Amsterdam, Netherlands)

    2023  Volume 17, Page(s) 100622

    Abstract: Identifying the role that host species play in pathogen transmission and maintenance is crucial for disease control, but it is a difficult task, in particular for vector-borne and multi-host pathogens, and especially when wildlife species are involved. ... ...

    Abstract Identifying the role that host species play in pathogen transmission and maintenance is crucial for disease control, but it is a difficult task, in particular for vector-borne and multi-host pathogens, and especially when wildlife species are involved. This is the case for a Crimean-Congo hemorrhagic fever virus (CCHFV) hotspot in north-eastern Spain, where Iberian ibex (
    Language English
    Publishing date 2023-08-23
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2834831-X
    ISSN 2352-7714
    ISSN 2352-7714
    DOI 10.1016/j.onehlt.2023.100622
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Risk factors associated with Streptococcus suis cases on pig farms in Spain.

    Neila-Ibáñez, Carlos / Napp, Sebastián / Pailler-García, Lola / Franco-Martínez, Lorena / Cerón, José Joaquín / Aragon, Virginia / Casal, Jordi

    The Veterinary record

    2023  Volume 193, Issue 5, Page(s) e3056

    Abstract: Background: Streptococcus suis can cause meningitis, polyarthritis and acute death in piglets. However, the risk factors associated with S. suis infection remain incompletely understood. Therefore, a longitudinal study was carried out, in which six ... ...

    Abstract Background: Streptococcus suis can cause meningitis, polyarthritis and acute death in piglets. However, the risk factors associated with S. suis infection remain incompletely understood. Therefore, a longitudinal study was carried out, in which six batches from two Spanish pig farms with S. suis problems were repeatedly examined to determine possible risk factors.
    Methods: A prospective case-control study was conducted, and potential risk factors were evaluated using mixed-effects logistic regression models. The explanatory variables included: (a) concomitant pathogens; (b) biomarkers associated with stress, inflammation and oxidative status; (c) farm environmental factors; and (d) parity and S. suis presence in sows. Three models were built to study the effect of these variables, including two to assess the risk factors involved in the subsequent development of disease.
    Results: Risk factors for S. suis-associated disease included porcine reproductive and respiratory syndrome virus co-infection at weaning (odds ratio [OR] = 6.69), sow parity (OR = 0.71), haptoglobin level before weaning (OR = 1.01), relative humidity (OR = 1.11) and temperature (OR = 0.13).
    Limitations: Laboratory diagnosis was done at the batch level, with individual diagnosis based on clinical signs only.
    Conclusions: This study confirms the multifactorial nature of S. suis-associated disease, with both environmental factors and factors related to the host involved in disease development. Controlling these factors may, therefore, help prevent the appearance of disease.
    MeSH term(s) Pregnancy ; Animals ; Swine ; Female ; Swine Diseases/epidemiology ; Streptococcus suis ; Farms ; Spain/epidemiology ; Longitudinal Studies ; Case-Control Studies ; Risk Factors ; Streptococcal Infections/epidemiology ; Streptococcal Infections/veterinary
    Language English
    Publishing date 2023-06-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 390015-0
    ISSN 2042-7670 ; 0042-4900
    ISSN (online) 2042-7670
    ISSN 0042-4900
    DOI 10.1002/vetr.3056
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Risk factors associated with Streptococcus suis cases on pig farms in Spain

    Neila‐Ibáñez, Carlos / Napp, Sebastián / Pailler‐García, Lola / Franco‐Martínez, Lorena / Cerón, José Joaquín / Aragon, Virginia / Casal, Jordi

    Veterinary Record. 2023 Sept. 9, v. 193, no. 5 p.e3056-

    2023  

    Abstract: BACKGROUND: Streptococcus suis can cause meningitis, polyarthritis and acute death in piglets. However, the risk factors associated with S. suis infection remain incompletely understood. Therefore, a longitudinal study was carried out, in which six ... ...

    Abstract BACKGROUND: Streptococcus suis can cause meningitis, polyarthritis and acute death in piglets. However, the risk factors associated with S. suis infection remain incompletely understood. Therefore, a longitudinal study was carried out, in which six batches from two Spanish pig farms with S. suis problems were repeatedly examined to determine possible risk factors. METHODS: A prospective case–control study was conducted, and potential risk factors were evaluated using mixed‐effects logistic regression models. The explanatory variables included: (a) concomitant pathogens; (b) biomarkers associated with stress, inflammation and oxidative status; (c) farm environmental factors; and (d) parity and S. suis presence in sows. Three models were built to study the effect of these variables, including two to assess the risk factors involved in the subsequent development of disease. RESULTS: Risk factors for S. suis‐associated disease included porcine reproductive and respiratory syndrome virus co‐infection at weaning (odds ratio [OR] = 6.69), sow parity (OR = 0.71), haptoglobin level before weaning (OR = 1.01), relative humidity (OR = 1.11) and temperature (OR = 0.13). LIMITATIONS: Laboratory diagnosis was done at the batch level, with individual diagnosis based on clinical signs only. CONCLUSIONS: This study confirms the multifactorial nature of S. suis‐associated disease, with both environmental factors and factors related to the host involved in disease development. Controlling these factors may, therefore, help prevent the appearance of disease.
    Keywords Streptococcus suis ; arthritis ; biomarkers ; case-control studies ; death ; farms ; haptoglobins ; inflammation ; laboratory diagnosis ; longitudinal studies ; meningitis ; mixed infection ; odds ratio ; porcine reproductive and respiratory syndrome ; regression analysis ; relative humidity ; risk ; risk assessment ; swine ; temperature ; viruses ; Spain
    Language English
    Dates of publication 2023-0909
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 390015-0
    ISSN 2042-7670 ; 0042-4900
    ISSN (online) 2042-7670
    ISSN 0042-4900
    DOI 10.1002/vetr.3056
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate

    Perlas, Albert / Bertran, Kateri / Abad, Francesc Xavier / Borrego, Carles M. / Nofrarías, Miquel / Valle, Rosa / Pailler-García, Lola / Ramis, Antonio / Cortey, Martí / Acuña, V. / Majó, Natàlia

    Science of the Total Environment. 2023 Mar., v. 863 p.160902-

    2023  

    Abstract: Avian influenza viruses (AIVs) can affect wildlife, poultry, and humans, so a One Health perspective is needed to optimize mitigation strategies. Migratory waterfowl globally spread AIVs over long distances. Therefore, the study of AIV persistence in ... ...

    Abstract Avian influenza viruses (AIVs) can affect wildlife, poultry, and humans, so a One Health perspective is needed to optimize mitigation strategies. Migratory waterfowl globally spread AIVs over long distances. Therefore, the study of AIV persistence in waterfowl staging and breeding areas is key to understanding their transmission dynamics and optimizing management strategies. Here, we used artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate (day/night cycles of photosynthetic active radiation and temperature, low water velocity, and similar microbiome to lowland rivers and stagnant water bodies) and then manipulated temperature and sediment presence (i.e., 10–13 °C vs. 16–18 °C, and presence vs. absence of sediments). An H1N1 low pathogenic AIV (LPAIV) strain was spiked in the streams, and water and sediment samples were collected at different time points until 14 days post-spike to quantify viral RNA and detect infectious particles. Viral RNA was detected until the end of the experiment in both water and sediment samples. In water samples, we observed a significant combined effect of temperature and sediments in viral decay, with higher viral genome loads in colder streams without sediments. In sediment samples, we didn't observe any significant effect of temperature. In contrast to prior laboratory-controlled studies that detect longer persistence times, infectious H1N1 LPAIV was isolated in water samples till 2 days post-spike, and none beyond. Infectious H1N1 LPAIV wasn't isolated from any sediment sample. Our results suggest that slow flowing freshwater surface waters may provide conditions facilitating bird-to-bird transmission for a short period when water temperature are between 10 and 18 °C, though persistence for extended periods (e.g., weeks or months) may be less likely. We hypothesize that experiments simulating real environments, like the one described here, provide a more realistic approach for assessing environmental persistence of AIVs.
    Keywords Influenza A virus ; Mediterranean climate ; One Health initiative ; RNA ; avian influenza ; environment ; environmental fate ; freshwater ; microbiome ; migratory birds ; photosynthetically active radiation ; poultry ; sediments ; viral genome ; water temperature ; waterfowl ; wildlife ; Influenza ; Birds ; Water ; Persistence ; Mediterranean
    Language English
    Dates of publication 2023-03
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2022.160902
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Evidence of Prolonged Crimean-Congo Hemorrhagic Fever Virus Endemicity by Retrospective Serosurvey, Eastern Spain.

    Carrera-Faja, Laura / Cardells, Jesús / Pailler-García, Lola / Lizana, Víctor / Alfaro-Deval, Gemma / Espunyes, Johan / Napp, Sebastian / Cabezón, Oscar

    Emerging infectious diseases

    2022  Volume 28, Issue 5, Page(s) 1031–1034

    Abstract: We conducted a retrospective serosurvey for antibodies against Crimean-Congo hemorrhagic fever virus in wild ungulates along the eastern Mediterranean Coast of Spain. The virus has been endemic in this region since 2010 but is mainly restricted to ... ...

    Abstract We conducted a retrospective serosurvey for antibodies against Crimean-Congo hemorrhagic fever virus in wild ungulates along the eastern Mediterranean Coast of Spain. The virus has been endemic in this region since 2010 but is mainly restricted to geographic clusters with extremely high seropositivity associated with high density of bovids.
    MeSH term(s) Hemorrhagic Fever Virus, Crimean-Congo ; Hemorrhagic Fever, Crimean/epidemiology ; Humans ; Retrospective Studies ; Spain/epidemiology
    Language English
    Publishing date 2022-04-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2805.212335
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71∆CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV-Study 2.

    Pujols, Joan / Blázquez, Elena / Segalés, Joaquim / Rodríguez, Fernando / Chang, Chia-Yu / Argilaguet, Jordi / Bosch-Camós, Laia / Rosell, Rosa / Pailler-García, Lola / Gavrilov, Boris / Campbell, Joy / Polo, Javier

    Vaccines

    2023  Volume 11, Issue 4

    Abstract: This study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71∆CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs acclimated to diets without or with 8% SDPP were ... ...

    Abstract This study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71∆CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs acclimated to diets without or with 8% SDPP were intranasally inoculated with 10
    Language English
    Publishing date 2023-04-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines11040825
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Crimean-Congo Hemorrhagic Fever Virus Seropositivity among Dromedary Camels, Algeria, 2020-2021.

    Guidoum, Khaled Azzedine / Carrera-Faja, Laura / Espunyes, Johan / Pailler-García, Lola / Benallou, Bouabdellah / Bouabdelli, Sarra / Smadi, Mustapha Adnane / Semara, Lounis / Cabezón, Oscar / Napp, Sebastián

    Emerging infectious diseases

    2023  Volume 29, Issue 12, Page(s) 2546–2548

    Abstract: Serosurvey results for Crimean-Congo hemorrhagic fever virus antibodies in dromedary camels in Algeria indicate that the pathogen is circulating endemically in desertic areas, despite the hostile environment. Thus, dromedaries are suitable sentinels for ... ...

    Abstract Serosurvey results for Crimean-Congo hemorrhagic fever virus antibodies in dromedary camels in Algeria indicate that the pathogen is circulating endemically in desertic areas, despite the hostile environment. Thus, dromedaries are suitable sentinels for detecting human risk for Crimean-Congo hemorrhagic fever in desertic areas.
    MeSH term(s) Animals ; Humans ; Hemorrhagic Fever Virus, Crimean-Congo ; Camelus ; Algeria/epidemiology ; Hemorrhagic Fever, Crimean/epidemiology ; Ixodidae
    Language English
    Publishing date 2023-11-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2912.230587
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Type I and III IFNs produced by the nasal epithelia and dimmed inflammation are features of alpacas resolving MERS-CoV infection.

    Te, Nigeer / Rodon, Jordi / Ballester, Maria / Pérez, Mónica / Pailler-García, Lola / Segalés, Joaquim / Vergara-Alert, Júlia / Bensaid, Albert

    PLoS pathogens

    2021  Volume 17, Issue 5, Page(s) e1009229

    Abstract: While MERS-CoV (Middle East respiratory syndrome Coronavirus) provokes a lethal disease in humans, camelids, the main virus reservoir, are asymptomatic carriers, suggesting a crucial role for innate immune responses in controlling the infection. ... ...

    Abstract While MERS-CoV (Middle East respiratory syndrome Coronavirus) provokes a lethal disease in humans, camelids, the main virus reservoir, are asymptomatic carriers, suggesting a crucial role for innate immune responses in controlling the infection. Experimentally infected camelids clear infectious virus within one week and mount an effective adaptive immune response. Here, transcription of immune response genes was monitored in the respiratory tract of MERS-CoV infected alpacas. Concomitant to the peak of infection, occurring at 2 days post inoculation (dpi), type I and III interferons (IFNs) were maximally transcribed only in the nasal mucosa of alpacas, while interferon stimulated genes (ISGs) were induced along the whole respiratory tract. Simultaneous to mild focal infiltration of leukocytes in nasal mucosa and submucosa, upregulation of the anti-inflammatory cytokine IL10 and dampened transcription of pro-inflammatory genes under NF-κB control were observed. In the lung, early (1 dpi) transcription of chemokines (CCL2 and CCL3) correlated with a transient accumulation of mainly mononuclear leukocytes. A tight regulation of IFNs in lungs with expression of ISGs and controlled inflammatory responses, might contribute to virus clearance without causing tissue damage. Thus, the nasal mucosa, the main target of MERS-CoV in camelids, seems central in driving an efficient innate immune response based on triggering ISGs as well as the dual anti-inflammatory effects of type III IFNs and IL10.
    MeSH term(s) Animals ; Antiviral Agents/metabolism ; Antiviral Agents/pharmacology ; Camelids, New World/immunology ; Camelids, New World/metabolism ; Camelids, New World/virology ; Chlorocebus aethiops ; Coronavirus Infections/immunology ; Coronavirus Infections/metabolism ; Coronavirus Infections/prevention & control ; Coronavirus Infections/veterinary ; Disease Reservoirs/veterinary ; Disease Resistance/drug effects ; Disease Resistance/genetics ; Disease Resistance/immunology ; Gene Expression Regulation ; Immunity, Innate/physiology ; Inflammation/immunology ; Inflammation/metabolism ; Inflammation/veterinary ; Inflammation/virology ; Interferon Type I/genetics ; Interferon Type I/metabolism ; Interferon Type I/pharmacology ; Interferons/genetics ; Interferons/metabolism ; Interferons/pharmacology ; Middle East Respiratory Syndrome Coronavirus/drug effects ; Middle East Respiratory Syndrome Coronavirus/immunology ; Middle East Respiratory Syndrome Coronavirus/physiology ; Nasal Mucosa/drug effects ; Nasal Mucosa/immunology ; Nasal Mucosa/metabolism ; Nasal Mucosa/virology ; Respiratory System/drug effects ; Respiratory System/immunology ; Respiratory System/metabolism ; Respiratory System/virology ; Vero Cells ; Viral Load/drug effects ; Virus Replication/drug effects
    Chemical Substances Antiviral Agents ; Interferon Type I ; interferon type III ; Interferons (9008-11-1)
    Language English
    Publishing date 2021-05-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7366
    ISSN (online) 1553-7374
    ISSN 1553-7366
    DOI 10.1371/journal.ppat.1009229
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain.

    Neila-Ibáñez, Carlos / Brogaard, Louise / Pailler-García, Lola / Martínez, Jorge / Segalés, Joaquim / Segura, Mariela / Heegaard, Peter M H / Aragon, Virginia

    Veterinary research

    2021  Volume 52, Issue 1, Page(s) 145

    Abstract: Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. ...

    Abstract Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals. Here, the role of innate immunity was studied in cesarean-derived colostrum-deprived piglets inoculated intranasally with either virulent S. suis strain 10 (S10) or non-virulent S. suis strain T15. Colonization of the inoculated piglets was confirmed at the end of the study by PCR and immunohistochemistry. Fever (≥40.5 °C) was more prevalent in piglets inoculated with S10 compared to T15 at 4 h after inoculation. During the 3 days of monitoring, no other major clinical signs were detected. Accordingly, only small changes in transcription of genes associated with the antibacterial innate immune response were observed at systemic sites, with S10 inducing an earlier response than T15 in blood. Local inflammatory response to the inoculation, evaluated by transcriptional analysis of selected genes in nasal swabs, was more sustained in piglets inoculated with the virulent S10, as demonstrated by transcription of inflammation-related genes, such as IL1B, IL1A, and IRF7. In contrast, most of the gene expression changes in trachea, lungs, and associated lymph nodes were observed in response to the non-virulent T15 strain. Thus, S. suis colonization in the absence of systemic infection induces an innate immune response in piglets that appears to be related to the virulence potential of the colonizing strain.
    MeSH term(s) Animals ; Immunity, Innate/immunology ; Streptococcal Infections/immunology ; Streptococcal Infections/veterinary ; Streptococcal Infections/virology ; Streptococcus suis/pathogenicity ; Swine ; Swine Diseases/immunology ; Swine Diseases/virology ; Virulence
    Language English
    Publishing date 2021-12-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 1146298-x
    ISSN 1297-9716 ; 0928-4249
    ISSN (online) 1297-9716
    ISSN 0928-4249
    DOI 10.1186/s13567-021-01013-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain

    Neila-Ibáñez, Carlos / Brogaard, Louise / Pailler-García, Lola / Martínez, Jorge / Segalés, Joaquim / Segura, Mariela / Heegaard, Peter M. H. / Aragon, Virginia

    BMC veterinary research. 2021 Dec., v. 52, no. 1

    2021  

    Abstract: Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. ...

    Abstract Streptococcus suis is a zoonotic pathogen of swine involved in arthritis, polyserositis, and meningitis. Colonization of piglets by S. suis is very common and occurs early in life. The clinical outcome of infection is influenced by the virulence of the S. suis strains and the immunity of the animals. Here, the role of innate immunity was studied in cesarean-derived colostrum-deprived piglets inoculated intranasally with either virulent S. suis strain 10 (S10) or non-virulent S. suis strain T15. Colonization of the inoculated piglets was confirmed at the end of the study by PCR and immunohistochemistry. Fever (≥40.5 °C) was more prevalent in piglets inoculated with S10 compared to T15 at 4 h after inoculation. During the 3 days of monitoring, no other major clinical signs were detected. Accordingly, only small changes in transcription of genes associated with the antibacterial innate immune response were observed at systemic sites, with S10 inducing an earlier response than T15 in blood. Local inflammatory response to the inoculation, evaluated by transcriptional analysis of selected genes in nasal swabs, was more sustained in piglets inoculated with the virulent S10, as demonstrated by transcription of inflammation-related genes, such as IL1B, IL1A, and IRF7. In contrast, most of the gene expression changes in trachea, lungs, and associated lymph nodes were observed in response to the non-virulent T15 strain. Thus, S. suis colonization in the absence of systemic infection induces an innate immune response in piglets that appears to be related to the virulence potential of the colonizing strain.
    Keywords Streptococcus suis ; arthritis ; blood ; fever ; gene expression ; immunohistochemistry ; inflammation ; innate immunity ; lymph ; meningitis ; nose ; pathogens ; piglets ; transcription (genetics) ; veterinary medicine ; virulence
    Language English
    Dates of publication 2021-12
    Size p. 145.
    Publishing place BioMed Central
    Document type Article
    ZDB-ID 1146298-x
    ISSN 1297-9716 ; 0928-4249
    ISSN (online) 1297-9716
    ISSN 0928-4249
    DOI 10.1186/s13567-021-01013-w
    Database NAL-Catalogue (AGRICOLA)

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