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  1. Article ; Online: Novel Mutations Reduce Expression of Meiotic Regulators SYCE1 and BOLL in Testis of Azoospermic Men from West Bengal, India.

    Pal, Samudra / Paladhi, Pranab / Dutta, Saurav / Mullick, Rupam Basu / Bose, Gunja / Ghosh, Papiya / Chattopadhyay, Ratna / Ghosh, Sujay

    Reproductive sciences (Thousand Oaks, Calif.)

    2023  Volume 31, Issue 4, Page(s) 1069–1088

    Abstract: We investigated the polymorphisms/mutations in synaptonemal complex central element protein 1 (SYCE1) and CDC25A mRNA-binding protein (BOLL) to test whether they increase the risk of azoospermia among Bengali-speaking men from West Bengal, India. Sanger' ... ...

    Abstract We investigated the polymorphisms/mutations in synaptonemal complex central element protein 1 (SYCE1) and CDC25A mRNA-binding protein (BOLL) to test whether they increase the risk of azoospermia among Bengali-speaking men from West Bengal, India. Sanger's dideoxy sequencing was used to genotype 140 azoospermic individuals who tested negative for Y chromosome microdeletion and 120 healthy controls. In both cases and controls, qRT-PCR was used to determine the expression summary of SYCE1 and BOLL. The perceived harmful consequences of identified mutations were inferred using in silico analysis. Suitable statistical approaches were used to conduct the association study. We found SYCE1 177insT (ON245141), 10650T > G (ON257012), 10093insT (ON257013), 10653insG (ON292504), rs10857748A > G, rs10857749G > A, and rs10857750T > A and BOLL 7708T > A (ON245141insT), rs72918816T > C, and rs700655C > T variants with the prevalence of azoospermia. Data from qRT-PCR and in silico studies projected that the variations would either disrupt the transcript's natural splice junctions or cause probable damage to the structure of the genes' proteins. SYCE1 gene variants [177insT (ON245141), 10650T > G (ON257012), 10093insT (ON257013), 10653insG (ON292504), rs10857748A > G, rs10857749G > A, rs10857750T > A] and BOLL gene variants [7708T > A (ON245141insT), rs72918816T > C, rs700655C > T] reduce the expression of respective gene in testicular tissue among azoospermic male as revealed from qRT-PCR result. These genetic variations could be utilized as screening tools for male infertility to determine the best course of treatment in routine ART practise.
    MeSH term(s) Humans ; Male ; Azoospermia/metabolism ; India ; Infertility, Male/metabolism ; Mutation ; Testis/metabolism
    Chemical Substances SYCP1 protein, human ; BOLL protein, human
    Language English
    Publishing date 2023-11-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2276411-2
    ISSN 1933-7205 ; 1933-7191
    ISSN (online) 1933-7205
    ISSN 1933-7191
    DOI 10.1007/s43032-023-01393-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4113: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Novel Mutations of TSPY1 Gene Associate Spermatogenic Failure Among Men.

    Paladhi, Pranab / Dutta, Saurav / Pal, Samudra / Bose, Gunja / Ghosh, Papiya / Chattopadhyay, Ratna / Chakravarty, Baidyanath / Saha, Indranil / Ghosh, Sujay

    Reproductive sciences (Thousand Oaks, Calif.)

    2022  Volume 29, Issue 4, Page(s) 1241–1261

    Abstract: Etiology of male infertility is intriguing owing to complex genetic regulation of human spermatogenesis and ethnic variations in genetic architecture of human populations. The present study characterizes the role of Y chromosome specific spermatogenic ... ...

    Abstract Etiology of male infertility is intriguing owing to complex genetic regulation of human spermatogenesis and ethnic variations in genetic architecture of human populations. The present study characterizes the role of Y chromosome specific spermatogenic regulator testis-specific protein Y-encoded 1 (TSPY1) gene mutation in spermatogenic failure. This case-control study includes 163 cases of spermatogenic failure and 175 age-matched fertile men as controls. We found five novel base substitutions, namely, MT162695, MN879413, MN889982, MN889983, MN719943, two deletions MN734578 and MN734579, three novel insertions MN719941, MN719942 and MN719944 through Sanger's dideoxy sequencing of TSPY1 gene reading frame. All these mutations exhibited strong association with male infertility. In silico analyses suggest prospective disruption in splice sites and alteration in different isoforms of TSPY1 transcripts and amino acid sequence in TSPY1 protein. The study provides novel evidence in favour of implication of TSPY1 gene in male fertility. The outcome sheds light to get insight into the issue of idiopathic male infertility in Bengali population.
    MeSH term(s) Case-Control Studies ; Cell Cycle Proteins/genetics ; Chromosomes, Human, Y ; Female ; Humans ; Infertility, Male/genetics ; Male ; Mutation ; Prospective Studies ; Spermatogenesis/genetics
    Chemical Substances Cell Cycle Proteins ; TSPY1 protein, human
    Language English
    Publishing date 2022-01-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2276411-2
    ISSN 1933-7205 ; 1933-7191
    ISSN (online) 1933-7205
    ISSN 1933-7191
    DOI 10.1007/s43032-021-00839-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4113: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Prevalence of Y chromosome microdeletion in azoospermia factor subregions among infertile men from West Bengal, India.

    Dutta, Saurav / Paladhi, Pranab / Pal, Samudra / Bose, Gunja / Ghosh, Papiya / Chattopadhyay, Ratna / Chakravarty, Baidyanath / Ghosh, Sujay

    Molecular genetics & genomic medicine

    2021  Volume 9, Issue 10, Page(s) e1769

    Abstract: Background: Etiology of male infertility is intriguing and Y chromosome microdeletion within azoospermia factor (AZF) sub-regions is considered major cause. We conducted a screening for Y chromosome microdeletion in an infertile male cohort from West ... ...

    Abstract Background: Etiology of male infertility is intriguing and Y chromosome microdeletion within azoospermia factor (AZF) sub-regions is considered major cause. We conducted a screening for Y chromosome microdeletion in an infertile male cohort from West Bengal, India to characterize Y chromosome microdeletion among infertile men.
    Methods: We recruited case subjects that were categorized on the basis of sperm count as azoospermia (N = 63), severe oligozoospermia (N = 38), and oligozoospermia (N = 17) and compared them with age, demography, and ethnicity matched healthy proven fertile control males (N = 84). Sequence Tagged Site makers and polymerase chain reaction based profiling of Y chromosome was done for AZF region and SRY for cases and controls.
    Results: We scored 16.1% of cases (19 out of 118) that bear one or more microdeletions in the studied loci and none among the controls. The aberrations were more frequent among azoospermic males (17 of 19) than in severe oligozoospermic subjects (2 of 19).
    Conclusion: Our study provides the results of screening of the largest Bengali infertile men sample genotyped with the maximum number of STS markers spanning the entire length of Y chromosome long arm. Y chromosome microdeletion is a significant genetic etiology of infertility among Bengali men.
    MeSH term(s) Azoospermia/genetics ; Case-Control Studies ; Chromosome Deletion ; Chromosomes, Human, Y/genetics ; Genetic Predisposition to Disease ; Humans ; India/epidemiology ; Infertility, Male/diagnosis ; Infertility, Male/epidemiology ; Infertility, Male/genetics ; Male ; Phenotype ; Prevalence ; Quantitative Trait Loci ; Sex Chromosome Aberrations ; Sex Chromosome Disorders of Sex Development/diagnosis ; Sex Chromosome Disorders of Sex Development/epidemiology ; Sex Chromosome Disorders of Sex Development/genetics
    Language English
    Publishing date 2021-08-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2734884-2
    ISSN 2324-9269 ; 2324-9269
    ISSN (online) 2324-9269
    ISSN 2324-9269
    DOI 10.1002/mgg3.1769
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Novel variations in spermatogenic transcription regulators RFX2 and TAF7 increase risk of azoospermia.

    Pal, Samudra / Paladhi, Pranab / Dutta, Saurav / Bose, Gunja / Ghosh, Papiya / Chattopadhyay, Ratna / Chakravarty, Baidyanath / Saha, Indranil / Ghosh, Sujay

    Journal of assisted reproduction and genetics

    2021  Volume 38, Issue 12, Page(s) 3195–3212

    Abstract: Purpose: Genetic etiology of idiopathic male infertility is enigmatic owing to involvement of multiple gene regulatory networks in spermatogenesis process. Any change in optimal function of the transcription factors involved in this process owing to ... ...

    Abstract Purpose: Genetic etiology of idiopathic male infertility is enigmatic owing to involvement of multiple gene regulatory networks in spermatogenesis process. Any change in optimal function of the transcription factors involved in this process owing to polymorphisms/mutations may increase the risk of infertility. We investigated polymorphisms/mutations of spermatogenic transcription regulators TAF7 and RFX2 and analysed their association with incidence of azoospermia among the men from West Bengal, India.
    Methods: Genotyping was carried by Sanger's dideoxy sequencing of 130 azoospermic men who were detected negative in Y chromosome microdeletion screening and 140 healthy controls. Association study was done by suitable statistical methods. In silico analysis was performed to infer the intuitive damaging effects of detected variants at transcripts and protein level.
    Results: We found significant association of TAF7 C16T (MW827584 G > A), RFX2 562delT (MZ560629delA), rs11547633 A > C, rs17606721 A > G, MW827583 C > T, and MZ379836 C > T variants with the incidence of azoospermia. In silico analysis predicted that the variants either alter the natural splice junctions of the transcript or cause probable damage in the structure of proteins of respective genes.
    Conclusion: Polymorphisms/mutations of TAF7 and RFX2 genes increase risk of male infertility in Bengali population. The novel variants may be used as markers for male infertility screening in ART practise.
    MeSH term(s) Azoospermia/genetics ; Chromosomes, Human, Y/genetics ; Humans ; India ; Infertility, Male/genetics ; Male ; Polymorphism, Genetic/genetics ; Regulatory Factor X Transcription Factors/genetics ; Spermatogenesis/genetics ; TATA-Binding Protein Associated Factors/genetics ; Transcription Factor TFIID/genetics
    Chemical Substances RFX2 protein, human ; Regulatory Factor X Transcription Factors ; TAF7 protein, human ; TATA-Binding Protein Associated Factors ; Transcription Factor TFIID
    Language English
    Publishing date 2021-11-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1112577-9
    ISSN 1573-7330 ; 1058-0468
    ISSN (online) 1573-7330
    ISSN 1058-0468
    DOI 10.1007/s10815-021-02352-5
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1985: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Evaluation of potency of the selected bioactive molecules from Indian medicinal plants with MPro of SARS-CoV-2 through in silico analysis

    Halder, Pinku / Pal, Upamanyu / Paladhi, Pranab / Dutta, Saurav / Paul, Pallab / Pal, Samudra / Das, Debasmita / Ganguly, Agnish / IshitaDutta / SayarneelMandal / Ray, Anirban / Ghosh, Sujay

    Journal of Ayurveda and integrative medicine. 2021 May 05,

    2021  

    Abstract: The recent outbreak of novel SARs CoVid-2 across the globe and absence of specific drug against this virus lead the scientific community to look into some alternative indigenous treatments. India as a hub of ayurvedic and medicinal plants can shed light ... ...

    Abstract The recent outbreak of novel SARs CoVid-2 across the globe and absence of specific drug against this virus lead the scientific community to look into some alternative indigenous treatments. India as a hub of ayurvedic and medicinal plants can shed light on its treatment using specific active bio-molecules from these plants.Keeping our herbal resources in mind we were interested to inquire whether some phytochemicals from Indian spices and medicinal plants can be used as alternative therapeutic agents in contrast to synthetic drugs.We used in-silico molecular docking approach to test whether bioactive molecules of herbal origin such as Hyperoside, Nimbaflavone, Ursolic acid, 6-gingerol, 6-shogaol& 6-paradol, Curcumin, Catechins&Epigallocatechin, α-Hederin, Piperine could bind and potentially block theMᵖʳᵒenzyme of Sars-CoV-2 virus.Ursolic acid showed the highest docking score (-8.7 kcal/mol) followed by Hyperoside (-8.6kcal/mol), α-Hederin (-8.5 kcal/mol) and Nimbaflavone (-8.0kcal/mol). Epigallocatechin, Catechins, and Curcumin also exhibited high binding affinity (Docking score -7.3, -7.1 and -7.1 kcal/mol) with the Mᵖʳᵒ. Rest of the tested phytochemicals exhibited moderate binding and inhibitory effects.This finding provides a basis for biochemical assay on Sars-CoV-2 virus.
    Keywords Ayurvedic medicine ; Severe acute respiratory syndrome coronavirus 2 ; computer simulation ; curcumin ; drugs ; epigallocatechin ; flavanols ; therapeutics ; ursolic acid ; viruses ; India
    Language English
    Dates of publication 2021-0505
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean ; Pre-press version
    ISSN 0975-9476
    DOI 10.1016/j.jaim.2021.05.003
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Evaluation of potency of the selected bioactive molecules from Indian medicinal plants with M

    Halder, Pinku / Pal, Upamanyu / Paladhi, Pranab / Dutta, Saurav / Paul, Pallab / Pal, Samudra / Das, Debasmita / Ganguly, Agnish / Dutta, Ishita / Mandal, Sayarneel / Ray, Anirban / Ghosh, Sujay

    Journal of Ayurveda and integrative medicine

    2021  Volume 13, Issue 2, Page(s) 100449

    Abstract: Background: The recent outbreak of the novel SARS-CoV-2 across the globe and the absence of specific drug against this virus lead the scientific community to look into some alternative indigenous treatments. India as a hub of Ayurvedic and medicinal ... ...

    Abstract Background: The recent outbreak of the novel SARS-CoV-2 across the globe and the absence of specific drug against this virus lead the scientific community to look into some alternative indigenous treatments. India as a hub of Ayurvedic and medicinal plants can shed light on its treatment using specific active bio-molecules from these plants.
    Objectives: Keeping our herbal resources in mind, we were interested to inquire whether some phytochemicals from Indian spices and medicinal plants can be used as alternative therapeutic agents in contrast to synthetic drugs.
    Materials and methods: We used
    Results: Ursolic acid showed the highest docking score (-8.7 kcal/mol) followed by hyperoside (-8.6 kcal/mol), α-Hederin (-8.5 kcal/mol) and nimbaflavone (-8.0 kcal/mol). epigallocatechin, catechins, and curcumin also exhibited high binding affinity (Docking score -7.3, -7.1 and -7.1 kcal/mol) with the M
    Conclusion: This finding provides a basis for biochemical assay of tested bioactive molecules on SARS-CoV-2 virus.
    Language English
    Publishing date 2021-05-21
    Publishing country United States
    Document type Journal Article
    ISSN 0975-9476
    ISSN 0975-9476
    DOI 10.1016/j.jaim.2021.05.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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