Article ; Online: Investigation of antimicrobial susceptibility and genetic diversity among Staphylococcus pseudintermedius isolated from dogs in Rio de Janeiro.
2023 Volume 13, Issue 1, Page(s) 20219
Abstract: Staphylococcus pseudintermedius is an opportunistic pathogen causing a variety of infections that are difficult to treat, especially because of the development of antimicrobial resistance. It has a clonal distribution around the world. To have a better ... ...
| Abstract | Staphylococcus pseudintermedius is an opportunistic pathogen causing a variety of infections that are difficult to treat, especially because of the development of antimicrobial resistance. It has a clonal distribution around the world. To have a better understanding of the MRSP population, we search the presence of MRSP in colonized or infected dogs. Samples from 99 dogs with infections and 35 from asymptomatic dogs were collected. Isolates were identified by mass spectrometry and Multiplex-PCR. The mecA gene was confirmed by conventional PCR. MRSP strains were analyzed by whole-genome sequencing. 75 S. pseudintermedius were identified, most from infection cases. The species were isolated from 70 out of the 135 dogs. Penicillin and Trimethoprim/Sulfamethoxazole presented higher resistance rates. Forty-seven strains were classified as multi-drug resistant (MDR), and were more isolated from dogs with infection (P < 0.05). Eighteen samples were classified as MRSP, representing 24.0% of the population. Six of 16 MRSP sequenced samples belonged to the world spread clone ST71; others belonged to unknown clones. Most samples carried the SCCmec type IIIA. Twenty-one different genetic resistance determinants were found among MRPS strains. MRSP is circulating among infected and colonized dogs in Rio de Janeiro, Brazil. |
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| MeSH term(s) | Dogs ; Animals ; Staphylococcal Infections/drug therapy ; Staphylococcal Infections/veterinary ; Staphylococcal Infections/epidemiology ; Anti-Bacterial Agents/pharmacology ; Methicillin Resistance ; Brazil ; Dog Diseases/drug therapy ; Dog Diseases/epidemiology ; Multiplex Polymerase Chain Reaction ; Genetic Variation ; Microbial Sensitivity Tests |
| Chemical Substances | Anti-Bacterial Agents |
| Language | English |
| Publishing date | 2023-11-18 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2615211-3 |
| ISSN | 2045-2322 ; 2045-2322 |
| ISSN (online) | 2045-2322 |
| ISSN | 2045-2322 |
| DOI | 10.1038/s41598-023-47549-z |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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