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  1. Article: Study on the Effect of Inter-Layer Cooling Time on Porosity and Melt Pool in Inconel 718 Components Processed by Laser Powder Bed Fusion.

    Baldi, Niccolò / Giorgetti, Alessandro / Palladino, Marco / Giovannetti, Iacopo / Arcidiacono, Gabriele / Citti, Paolo

    Materials (Basel, Switzerland)

    2023  Volume 16, Issue 11

    Abstract: This paper investigates the effects on the material microstructure of varying the Inter-Layer Cooling Time (ILCT) during the printing process in laser powder bed fusion (L-PBF) multi-laser machines. Despite these machines allowing higher productivity ... ...

    Abstract This paper investigates the effects on the material microstructure of varying the Inter-Layer Cooling Time (ILCT) during the printing process in laser powder bed fusion (L-PBF) multi-laser machines. Despite these machines allowing higher productivity rates compared to single laser machines, they are affected by lower ILCT values, which could be critical for material printability and microstructure. The ILCT values depend both on the process parameter sets and design choices for the parts and play an important role in the Design for Additive Manufacturing approach in L-PBF process. In order to identify the critical range of ILCT for this working condition, an experimental campaign is presented on the nickel-based superalloy Inconel 718, which is widely used for the printing of turbomachinery components. The effect of ILCT on the microstructure of the material is evaluated in terms of porosity and melt pool analysis on printed cylinder specimens, considering ILCT decreasing and increasing in the range of 22 to 2 s. The experimental campaign shows that an ILCT of less than 6 s introduces criticality in the material microstructure. In particular, at an ILCT value of 2 s, widespread keyhole porosity (close to 1‱) and critical and deeper melt pool (about 200 microns depth) are measured. This variation in melt pool shape indicates a change in the powder melting regime and, consequently, modifications of the printability window promoting the expansion of the keyhole region. In addition, specimens with geometry obstructing the heat flow have been studied using the critical ILCT value (2 s) to evaluate the effect of the surface-to-volume ratio. The results show an enhancement of the porosity value (about 3‱), while this effect is limited for the depth of the melt pool.
    Language English
    Publishing date 2023-05-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma16113920
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Serum Cardiac Biomarkers in Asymptomatic Hemodialysis Patients: Role of Soluble Suppression of Tumorigenicity-2.

    Mancianti, Nicoletta / Maresca, Barbara / Palladino, Marco / Salerno, Gerardo / Cardelli, Patrizia / Menè, Paolo / Barberi, Simona

    Blood purification

    2021  Volume 51, Issue 2, Page(s) 155–162

    Abstract: Introduction: Cardiovascular events (CVE) remain the leading cause of mortality in hemodialysis (HD) patients. The ability to assess the risk of short-term CVE is of great importance. Soluble suppression of tumorogenicity-2 (sST2) is a novel biomarker ... ...

    Abstract Introduction: Cardiovascular events (CVE) remain the leading cause of mortality in hemodialysis (HD) patients. The ability to assess the risk of short-term CVE is of great importance. Soluble suppression of tumorogenicity-2 (sST2) is a novel biomarker that better stratifies risk of CVE than troponins in patients with heart failure. Few studies have investigated the role of sST2 in the HD population. The aim of this single-center study was to assess the predictive ability of sST2 on CVE in comparison to high-sensitive cardiac troponin I (hs-cTnI) and B-type natriuretic peptide (BNP) in HD patients.
    Methods: This study used a prospective, observational cohort design. We enrolled 40 chronic HD patients asymptomatic for chest pain and without recent history of acute coronary syndrome. We tested sST2 pre-/post-HD, hs-cTnI, and BNP. Demographic/dialytic/echocardiographic data were evaluated. We recorded the number of CVE for 12 months. The patients were classified into 2 groups: those who developed CVE and those who did not.
    Results: Ten of the 40 patients (25%) developed CVE during a 12-month follow-up. Increased sST2 levels (p < 0.0001) as well as hs-cTnI and BNP are predictive of CVE. When analyzing biomarkers as binary variables for values above or below the normal range, the correlation remained significant only for sST2 (p = 0.001). A small variation in sST2 levels before and after HD sessions was found (-2.1 ng/mL). sST2 was correlated with left ventricular (LV) echocardiographic data: LV mass index (p = 0.0001), LV ejection fraction (p = 0.01), and diastolic bulging of septum (p = 0.015). BNP and sST2 combination increased the prediction of CVE in a statistical model.
    Conclusion: Our study confirms that sST2 is useful for stratifying CV risk in the HD population. sST2 can be evaluated simply as a dichotomous value higher or lower than the normal range, making it easily interpretable. Dialysis and residual diuresis did not affect significantly sST2. A multimarker approach that incorporates sST2 and BNP may improve the prediction of CVE.
    MeSH term(s) Biomarkers ; Humans ; Interleukin-1 Receptor-Like 1 Protein ; Natriuretic Peptide, Brain ; Prognosis ; Prospective Studies ; Renal Dialysis/adverse effects
    Chemical Substances Biomarkers ; Interleukin-1 Receptor-Like 1 Protein ; Natriuretic Peptide, Brain (114471-18-0)
    Language English
    Publishing date 2021-04-28
    Publishing country Switzerland
    Document type Journal Article ; Observational Study
    ZDB-ID 605548-5
    ISSN 1421-9735 ; 0253-5068
    ISSN (online) 1421-9735
    ISSN 0253-5068
    DOI 10.1159/000515675
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: [SARS CoV-2 related disease features in a population of chronic hemodialysis patients].

    Silvestri, Cristina / Di Stante, Silvio / Bertuzzi, Veronica / Martello, Mauro / Palladino, Marco / Grabocka, Xhensila / Kulurianu, Hrissanthi / Manenti, Flavia / Pizzolante, Francesca / Cardillo, Assunta / Francioso, Angelo / Paci Della Costanza, Osmy / Valentini, Chiara / Di Luca, Marina

    Giornale italiano di nefrologia : organo ufficiale della Societa italiana di nefrologia

    2020  Volume 37, Issue 6

    Abstract: Patients on chronic dialysis have an increased risk for SARS CoV-2 virus disease and its complications because of multiple comorbidities and alterations in the immune response caused by renal disease. In this retrospective observational study we describe ...

    Abstract Patients on chronic dialysis have an increased risk for SARS CoV-2 virus disease and its complications because of multiple comorbidities and alterations in the immune response caused by renal disease. In this retrospective observational study we describe the clinical features and the evolution of SARS CoV-2-related disease in 19 patients of our Pesaro and Fano facilities, where incidence and mortality of the epidemic were among the highest in Italy. A total of 176 patients were undergoing chronic treatment, 153 hemodialysis and 23 peritoneal dialysis. The incidence of infection was 10,8%, with 84% needing hospitalization and mortality amounting to 53%. The most frequent onset symptom was fever (84,2%) and the most used therapy was an association of low molecular weight heparin and hydroxychloroquine (57,9%). Comparing the deceased and survivor populations we noticed significant differences in age and presence of cardiopathy for what concerns anamnestic data and in fatigue and dyspnea in terms of clinical presentation. LDH and CPK resulted highest among deceased patients, while the use of enoxaparin was more frequent in survivors. By observing contagions over time, we also noticed that most of the cases, and the ones with worse clinical condition and outcome, all occurred in the early stage of the epidemic and in particular within the first 20 days from the implementation and codification of the measures to prevent its spread, the only modifiable factor that had an unmistakable effect on the evolution of events.
    MeSH term(s) Aged ; Aged, 80 and over ; COVID-19/diagnostic imaging ; COVID-19/drug therapy ; COVID-19/epidemiology ; COVID-19/prevention & control ; COVID-19/therapy ; COVID-19 Testing ; Combined Modality Therapy ; Comorbidity ; Humans ; Infection Control ; Italy/epidemiology ; Kaplan-Meier Estimate ; Kidney Failure, Chronic/epidemiology ; Kidney Failure, Chronic/therapy ; Middle Aged ; Pandemics ; Proportional Hazards Models ; Renal Dialysis ; Retrospective Studies ; SARS-CoV-2 ; Symptom Assessment ; Tomography, X-Ray Computed
    Language Italian
    Publishing date 2020-12-07
    Publishing country Italy
    Document type Journal Article ; Multicenter Study ; Observational Study
    ZDB-ID 1237110-5
    ISSN 1724-5990 ; 0393-5590
    ISSN (online) 1724-5990
    ISSN 0393-5590
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: HDAC1 inhibition by MS-275 in mesothelial cells limits cellular invasion and promotes MMT reversal.

    Rossi, Lucia / Battistelli, Cecilia / de Turris, Valeria / Noce, Valeria / Zwergel, Clemens / Valente, Sergio / Moioli, Alessandra / Manzione, Andrea / Palladino, Marco / Bordoni, Veronica / Domenici, Alessandro / Menè, Paolo / Mai, Antonello / Tripodi, Marco / Strippoli, Raffaele

    Scientific reports

    2018  Volume 8, Issue 1, Page(s) 8492

    Abstract: Peritoneal fibrosis is a pathological alteration of the peritoneal membrane occurring in a variety of conditions including peritoneal dialysis (PD), post-surgery adhesions and peritoneal metastases. The acquisition of invasive and pro-fibrotic abilities ... ...

    Abstract Peritoneal fibrosis is a pathological alteration of the peritoneal membrane occurring in a variety of conditions including peritoneal dialysis (PD), post-surgery adhesions and peritoneal metastases. The acquisition of invasive and pro-fibrotic abilities by mesothelial cells (MCs) through induction of MMT, a cell-specific form of EMT, plays a main role in this process. Aim of this study was to evaluate possible effects of histone deacetylase (HDAC) inhibitors, key components of the epigenetic machinery, in counteracting MMT observed in MCs isolated from effluent of PD patients. HDAC inhibitors with different class/isoform selectivity have been used for pharmacological inhibition. While the effect of other inhibitors was limited to a partial E-cadherin re-expression, MS-275, a HDAC1-3 inhibitor, promoted: (i) downregulation of mesenchymal markers (MMP2, Col1A1, PAI-1, TGFβ1, TGFβRI) (ii) upregulation of epithelial markers (E-cadherin, Occludin), (iii) reacquisition of an epithelial-like morphology and (iv) marked reduction of cellular invasiveness. Results were confirmed by HDAC1 genetic silencing. Mechanistically, MS-275 causes: (i) increase of nuclear histone H3 acetylation (ii) rescue of the acetylation profile on E-cadherin promoter, (iii) Snail functional impairment. Overall, our study, pinpointing a role for HDAC1, revealed a new player in the regulation of peritoneal fibrosis, providing the rationale for future therapeutic opportunities.
    MeSH term(s) Aged ; Aged, 80 and over ; Benzamides/pharmacology ; Cadherins/metabolism ; Cell Movement/drug effects ; Epithelial Cells/cytology ; Epithelial Cells/metabolism ; Epithelial-Mesenchymal Transition/drug effects ; Female ; Histone Deacetylase 1/antagonists & inhibitors ; Histone Deacetylase 1/genetics ; Histone Deacetylase 1/metabolism ; Histone Deacetylase Inhibitors/pharmacology ; Humans ; Kidney Failure, Chronic/metabolism ; Kidney Failure, Chronic/pathology ; Male ; Middle Aged ; Peritoneal Dialysis ; Peritoneum/cytology ; Pyridines/pharmacology ; RNA Interference ; RNA, Small Interfering/metabolism ; Snail Family Transcription Factors/metabolism ; Transforming Growth Factor beta1/metabolism
    Chemical Substances Benzamides ; Cadherins ; Histone Deacetylase Inhibitors ; Pyridines ; RNA, Small Interfering ; SNAI1 protein, human ; Snail Family Transcription Factors ; Transforming Growth Factor beta1 ; entinostat (1ZNY4FKK9H) ; HDAC1 protein, human (EC 3.5.1.98) ; Histone Deacetylase 1 (EC 3.5.1.98)
    Language English
    Publishing date 2018-05-31
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-018-26319-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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