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  1. Article: Reply to the Comment on "Melanisation of Aspergillus terreus-Is Butyrolactone I Involved in the Regulation of Both DOPA and DHN Types of Pigments in Submerged Culture? Microorganisms 2017, 5, 22".

    Palonen, Elina K / Raina, Sheetal / Brandt, Annika / Meriluoto, Jussi / Keshavarz, Tajalli / Soini, Juhani T

    Microorganisms

    2017  Volume 5, Issue 3

    Abstract: n/a. ...

    Abstract n/a.
    Language English
    Publishing date 2017-07-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms5030036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Melanisation of Aspergillus terreus-Is Butyrolactone I Involved in the Regulation of Both DOPA and DHN Types of Pigments in Submerged Culture?

    Palonen, Elina K / Raina, Sheetal / Brandt, Annika / Meriluoto, Jussi / Keshavarz, Tajalli / Soini, Juhani T

    Microorganisms

    2017  Volume 5, Issue 2

    Abstract: Pigments and melanins of fungal spores have been investigated for decades, revealing important roles in the survival of the fungus in hostile environments. The key genes and the encoded enzymes for pigment and melanin biosynthesis have recently been ... ...

    Abstract Pigments and melanins of fungal spores have been investigated for decades, revealing important roles in the survival of the fungus in hostile environments. The key genes and the encoded enzymes for pigment and melanin biosynthesis have recently been found in Ascomycota, including
    Language English
    Publishing date 2017-05-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms5020022
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Transcriptomic Complexity of Aspergillus terreus Velvet Gene Family under the Influence of Butyrolactone I.

    Palonen, Elina K / Raina, Sheetal / Brandt, Annika / Meriluoto, Jussi / Keshavarz, Tajalli / Soini, Juhani T

    Microorganisms

    2017  Volume 5, Issue 1

    Abstract: Filamentous fungi of the Ascomycota phylum are known to contain a family of conserved conidiation regulating proteins with distinctive velvet domains. In Aspergilli, this velvet family includes four proteins, VeA, VelB, VelC and VosA, and is involved in ... ...

    Abstract Filamentous fungi of the Ascomycota phylum are known to contain a family of conserved conidiation regulating proteins with distinctive velvet domains. In Aspergilli, this velvet family includes four proteins, VeA, VelB, VelC and VosA, and is involved in conidiation and secondary metabolism along with a global regulator LaeA. In A. terreus, the overexpression of LaeA has been observed to increase the biogenesis of the pharmaceutically-important secondary metabolite, lovastatin, while the role of the velvet family has not been studied. The secondary metabolism and conidiation of A. terreus have also been observed to be increased by butyrolactone I in a quorum-sensing manner. An enlightenment of the interplay of these regulators will give potential advancement to the industrial use of this fungus, as well as in resolving the pathogenic features. In this study, the Aspergillus terreus MUCL 38669 transcriptome was strand-specifically sequenced to enable an in-depth gene expression analysis to further investigate the transcriptional role of butyrolactone I in these processes. The sequenced transcriptome revealed intriguing properties of the velvet family transcripts, including the regulator laeA, and uncovered the velC gene in A. terreus. The reliability refining microarray gene expression analysis disclosed a positive regulatory role for butyrolactone I in laeA expression, as well as an influence on the expression of the canonical conidiation-regulating genes under submerged culture. All of this supports the suggested regulative role of butyrolactone I in A. terreus secondary metabolism, as well as conidiation.
    Language English
    Publishing date 2017-03-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms5010012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Butyrolactone I Quantification from Lovastatin Producing Aspergillus terreus Using Tandem Mass Spectrometry-Evidence of Signalling Functions.

    Palonen, Elina K / Neffling, Milla-Riina / Raina, Sheetal / Brandt, Annika / Keshavarz, Tajalli / Meriluoto, Jussi / Soini, Juhani

    Microorganisms

    2014  Volume 2, Issue 2, Page(s) 111–127

    Abstract: Aspergillus terreus is an industrially important filamentous fungus producing a wide spectrum of secondary metabolites, including lovastatin and itaconic acid. It also produces butyrolactone I which has shown potential as an antitumour agent. ... ...

    Abstract Aspergillus terreus is an industrially important filamentous fungus producing a wide spectrum of secondary metabolites, including lovastatin and itaconic acid. It also produces butyrolactone I which has shown potential as an antitumour agent. Additionally, butyrolactone I has been implicated to have a regulating role in the secondary metabolism and morphology of A. terreus. In this study, a quantitative time-course liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS-MS) analysis of butyrolactone I is reported for the first time in nine-day long submerged cultures of A. terreus. Butyrolactone I was fragmented in the mass analysis producing a reproducible fragmentation pattern of four main daughter ions (m/z 307, 331, 363 and 393) in all the samples tested. Supplementing the cultures with 100 nM butyrolactone I caused a statistically significant increase (up to two-fold) in its production, regardless of the growth stage but was constitutive when butyrolactone I was added at high cell density during the stationary phase. Furthermore, the extracellular butyrolactone I concentration peaked at 48 h post inoculation, showing a similar profile as has been reported for bacterial quorum sensing molecules. Taken together, the results support the idea of butyrolactone I as a quorum sensing molecule in A. terreus.
    Language English
    Publishing date 2014-06-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms2020111
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Membrane curvature effects on glycolipid transfer protein activity.

    Nylund, Matts / Fortelius, Christina / Palonen, Elina K / Molotkovsky, Julian G / Mattjus, Peter

    Langmuir : the ACS journal of surfaces and colloids

    2007  Volume 23, Issue 23, Page(s) 11726–11733

    Abstract: The glycolipid transfer protein (GLTP) is monomeric in aqueous solutions, and it binds weakly to membrane interfaces with or without glycolipids. GLTP is a surface-active protein and adsorbs to exert a maximal surface pressure value of 19 mN/m. The ... ...

    Abstract The glycolipid transfer protein (GLTP) is monomeric in aqueous solutions, and it binds weakly to membrane interfaces with or without glycolipids. GLTP is a surface-active protein and adsorbs to exert a maximal surface pressure value of 19 mN/m. The change in surface pressure following GLTP adsorption decreased linearly with initial surface pressure. The exclusion pressure for different phospholipids and sphingolipids was between 23 and 31 mN/m, being clearly highest for the negatively charged dipalmitoyl-phosphatidylserine. This can be explained by electrostatic forces when GLTP is positively charged at neutral pH (isoelectric point = 9.0) and by phosphatidylserine being negatively charged. If GLTP is injected under a palmitoyl-galactosylceramide monolayer above 30 mN/m, the presence of GLTP leads to a decrease in the surface pressure as a function of time. This suggests that GLTP is able to remove glycolipids from the monolayer without penetrating the monolayer. On the other hand, if phospholipid vesicles with or without glycolipids are also present in the subphase, no change in the surface pressure takes place. This suggests that GLTP in the presence of curved membranes is not able to transfer from or to planar membranes. We also show that transfer of fluorescently labeled galactosylceramide is faster from small highly curved palmitoyl-oleoyl-phosphatidylcholine and dipalmitoyl-phosphatidylcholine bilayer vesicles but not from palmitoyl-sphingomyelin vesicles regardless of the size.
    MeSH term(s) 1,2-Dipalmitoylphosphatidylcholine/chemistry ; 1,2-Dipalmitoylphosphatidylcholine/metabolism ; Adsorption ; Carrier Proteins/chemistry ; Carrier Proteins/metabolism ; Galactosylceramides/chemistry ; Galactosylceramides/metabolism ; Glycolipids/chemistry ; Glycolipids/metabolism ; Hydrogen-Ion Concentration ; Isoelectric Point ; Kinetics ; Membrane Microdomains/chemistry ; Membrane Microdomains/metabolism ; Phosphatidylserines/chemistry ; Phosphatidylserines/metabolism ; Phospholipids/chemistry ; Phospholipids/metabolism ; Scattering, Radiation ; Spectrometry, Fluorescence ; Sphingolipids/chemistry ; Sphingolipids/metabolism ; Static Electricity ; Surface Properties ; Temperature
    Chemical Substances Carrier Proteins ; Galactosylceramides ; Glycolipids ; Phosphatidylserines ; Phospholipids ; Sphingolipids ; lipid transfer protein ; 1,2-Dipalmitoylphosphatidylcholine (2644-64-6) ; dipalmitoylphosphatidylserine (3036-82-6)
    Language English
    Publishing date 2007-11-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2005937-1
    ISSN 1520-5827 ; 0743-7463
    ISSN (online) 1520-5827
    ISSN 0743-7463
    DOI 10.1021/la701927u
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Is quorum sensing involved in lovastatin production in the filamentous fungus Aspergillus terreus?

    Raina, Sheetal / De Vizio, Daniela / Palonen, Elina K. / Odell, Mark / Brandt, Annika M. / Soini, Juhani T. / Keshavarz, Tajalli

    Process biochemistry

    Volume v. 47,, Issue no. 5

    Abstract: A novel role for butyrolactone I as a quorum sensing molecule in the filamentous fungus Aspergillus terreus is proposed based on its ability to affect both lovastatin and its own production. In this work, the exogenous addition of 100nM butyrolactone I ... ...

    Abstract A novel role for butyrolactone I as a quorum sensing molecule in the filamentous fungus Aspergillus terreus is proposed based on its ability to affect both lovastatin and its own production. In this work, the exogenous addition of 100nM butyrolactone I at 96h post-inoculation to submerged cultures of A. terreus in 5L bioreactors resulted in 2.5-fold increase in lovastatin production as compared to control cultures at 168h. An increase in endogenous butyrolactone I levels (2.5-fold) is also detected in cultures supplemented with butyrolactone I, thus suggesting an auto-stimulatory function. Molecular analysis of butyrolactone I-mediated enhancement of lovastatin production revealed induction of lovastatin biosynthetic genes, lovB and lovF, at the transcriptional level. Microarray analysis of A. terreus transcriptome depicted a growth phase-specific response to butyrolactone I addition as the majority of the expressed genes showed differential expression during the specific growth phase (p<0.01). This study demonstrates for the first time the potential of butyrolactone I as a growth phase-specific inducer of the secondary metabolite lovastatin production and shows the auto-stimulatory effect of this molecule on its own production in the filamentous fungus A. terreus.
    Keywords fungi ; microarray technology ; secondary metabolites ; quorum sensing ; genes ; bioreactors ; gene expression ; transcriptome ; Aspergillus terreus ; transcription (genetics) ; lovastatin ; gene expression regulation
    Language English
    Document type Article
    ISSN 1359-5113
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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