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  1. Article ; Online: Long-Term Preservation and Storage of Faecal Samples in Whatman ® Cards for PCR Detection and Genotyping of Giardia duodenalis and Cryptosporidium hominis

    Pamela Carolina Köster / Begoña Bailo / Alejandro Dashti / Carolina Hernández-Castro / Rafael Calero-Bernal / Francisco Ponce-Gordo / David González-Barrio / David Carmena

    Animals, Vol 11, Iss 1369, p

    2021  Volume 1369

    Abstract: Preservation and conservation of biological specimens, including faecal samples, is a challenge in remote areas or poor-resource settings where the cold chain cannot be maintained. This study aims at evaluating the suitability of filter cards for long- ... ...

    Abstract Preservation and conservation of biological specimens, including faecal samples, is a challenge in remote areas or poor-resource settings where the cold chain cannot be maintained. This study aims at evaluating the suitability of filter cards for long-term storage of faecal samples of animal and human origin positive to the diarrhoea-causing protozoan parasites, Giardia duodenalis and Cryptosporidium hominis . Three commercially available Whatman ® Filter Cards were comparatively assessed: the FTA ® Classic Card, the FTA ® Elute Micro Card, and the 903 Protein Saver Card. Human faecal samples positive to G. duodenalis ( n = 5) and C. hominis ( n = 5) were used to impregnate the selected cards at given storage (1 month, 3 months, and 6 months) periods and temperature (−20 °C, 4 °C, and room temperature) conditions. Parasite DNA was detected by PCR-based methods. Sensitivity assays and quality control procedures to assess suitability for genotyping purposes were conducted. Overall, all three Whatman ® cards were proven useful for the detection and molecular characterisation of G. duodenalis and C. hominis under the evaluated conditions. Whatman ® cards represent a simple, safe, and cost-effective option for the transportation, preservation, and storage of faecal samples without the need of the cold chain.
    Keywords filter card ; faeces ; transportation ; storage ; preservation ; Giardia duodenalis ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 333
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Molecular Diversity of Giardia duodenalis , Cryptosporidium spp. and Blastocystis sp. in Asymptomatic School Children in Leganés, Madrid (Spain)

    Aly Salimo Muadica / Pamela Carolina Köster / Alejandro Dashti / Begoña Bailo / Marta Hernández-de-Mingo / Lucia Reh / Sooria Balasegaram / Neville Q Verlander / Esther Ruiz Chércoles / David Carmena

    Microorganisms, Vol 8, Iss 4, p

    2020  Volume 466

    Abstract: Enteric parasites including Giardia duodenalis , Cryptosporidium spp., and to a lesser extent, Blastocystis sp. and Enterocytozoon bieneusi , are major worldwide contributors to diarrhoeal disease. Assessing their molecular frequency and diversity is ... ...

    Abstract Enteric parasites including Giardia duodenalis , Cryptosporidium spp., and to a lesser extent, Blastocystis sp. and Enterocytozoon bieneusi , are major worldwide contributors to diarrhoeal disease. Assessing their molecular frequency and diversity is important to ascertain the sources of infection, transmission dynamics, and zoonotic potential. Little molecular information is available on the genotypes of these pathogens circulating in apparently healthy children. Here, we show that asymptomatic carriage of G. duodenalis (17.4%, 95% CI: 15.5‒19.4%), Blastocystis sp. (13.0%, 95% CI: 11.4‒14.8%), and Cryptosporidium spp. (0.9%, 95% CI: 0.5‒1.5%) is common in children (1‒16 years; n = 1512) from Madrid, Spain. Our genotyping data indicate that; (i) the observed frequency and diversity of parasite genetic variants are very similar to those previously identified in Spanish clinical samples, so that the genotype alone does not predict the clinical outcome of the infection, (ii) anthroponotic transmission accounts for a large proportion of the detected cases, highlighting that good personal hygiene practices are important to minimizing the risk of infection, (iii) Blastocystis ST4 may represent a subtype of the parasite with higher pathogenic potential, and (iv) Enterocytozoon bieneusi does not represent a public health concern in healthy children.
    Keywords giardia ; cryptosporidium ; blastocystis ; enterocytozoon ; asymptomatic children ; pcr ; molecular detection ; molecular epidemiology ; genotyping ; spain ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2020-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Multilocus Genotyping of Giardia duodenalis in Mostly Asymptomatic Indigenous People from the Tapirapé Tribe, Brazilian Amazon

    Pamela Carolina Köster / Antonio F. Malheiros / Jeffrey J. Shaw / Sooria Balasegaram / Alexander Prendergast / Héloïse Lucaccioni / Luciana Melhorança Moreira / Larissa M. S. Lemos / Alejandro Dashti / Begoña Bailo / Arlei Marcili / Herbert Sousa Soares / Solange Maria Gennari / Rafael Calero-Bernal / David González-Barrio / David Carmena

    Pathogens, Vol 10, Iss 2, p

    2021  Volume 206

    Abstract: Little information is available on the occurrence and genetic variability of the diarrhoea-causing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey describes the frequency, ... ...

    Abstract Little information is available on the occurrence and genetic variability of the diarrhoea-causing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey describes the frequency, genotypes, and risk associations for this pathogen in Tapirapé people (Brazilian Amazon) at four sampling campaigns during 2008–2009. Microscopy was used as a screening test, and molecular (PCR and Sanger sequencing) assays targeting the small subunit ribosomal RNA, the glutamate dehydrogenase, the beta-giardin, and the triosephosphate isomerase genes as confirmatory/genotyping methods. Associations between G. duodenalis and sociodemographic and clinical variables were investigated using Chi-squared test and univariable/multivariable logistic regression models. Overall, 574 individuals belonging to six tribes participated in the study, with G. duodenalis prevalence rates varying from 13.5–21.7%. The infection was positively linked to younger age and tribe. Infected children <15 years old reported more frequent gastrointestinal symptoms compared to adults. Assemblage B accounted for three out of four G. duodenalis infections and showed a high genetic diversity. No association between assemblage and age or occurrence of diarrhoea was demonstrated. These data indicate that the most likely source of infection was anthropic and that different pathways (e.g., drinking water) may be involved in the transmission of the parasite.
    Keywords Giardia ; Brazil ; Amazon ; asymptomatic ; community ; genotyping ; Medicine ; R
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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