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Article ; Online: Can carbon emission trading improve regional eco-efficiency? Based on the environmental innovation perspective.

Li, Xiongying / Yu, Yifan / Pan, Junhua / Bhuiyan, Miraj Ahmed

Environmental science and pollution research international

2024

Abstract: This paper uses green innovation and environmental pollution as the mediating variables to construct a mediating effect model to investigate whether China's carbon emission trading policy can improve regional eco-efficiency by reducing regional ... ...

Abstract	This paper uses green innovation and environmental pollution as the mediating variables to construct a mediating effect model to investigate whether China's carbon emission trading policy can improve regional eco-efficiency by reducing regional environmental pollution and stimulating green innovation. This study is based on panel data from 30 provinces and municipalities directly under China's central government and autonomous regions from 2003 to 2019. The eco-efficiency of these provinces is measured using the super-efficiency DEA model and the difference-in-difference method (DID). The results show that (1) China's emission trading policy significantly improves regional ecological efficiency and the per capita GDP. The improvement can effectively improve regional ecological efficiency. (2) The mediating effect of green innovation and environmental pollution is significant. That is, China's carbon emission trading policy further improves regional ecological efficiency by stimulating more green innovation and reducing the synergy brought by environmental pollution. (3) There are differences in the feedback of this impact mechanism between different regions: It shows the characteristics of the western region being more significant than the eastern region. The central region has no significant effect. The research conclusion can provide a policy reference for the subsequent unified promotion of the construction of a carbon emission trading market nationwide and a theoretical basis for helping to achieve the "dual carbon" goal.
Language	English
Publishing date	2024-04-16
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-024-33102-6
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Article: Modified xiaoyao san combined with chemotherapy for breast cancer: A systematic review and meta-analysis of randomized controlled trials.

Pan, Junhua / Fu, Shunlian / Zhou, Qian / Lin, Dajun / Chen, Qiu

Frontiers in oncology

2023 Volume 13, Page(s) 1050337

Abstract: Background: Breast cancer is a common cause of cancer-related death worldwide. Chemotherapy plays an indispensable role in the conventional treatment of breast cancer, bringing some physical burdens and discomfort on cancer patients. Consequently, more ... ...

Abstract	Background: Breast cancer is a common cause of cancer-related death worldwide. Chemotherapy plays an indispensable role in the conventional treatment of breast cancer, bringing some physical burdens and discomfort on cancer patients. Consequently, more and more patients turn to seeking the help of Complementary and Alternative Medicine (CAM), mainly traditional Chinese medicine (TCM). Xiaoyao san (XYS), a classical formula, has been shown to improve symptoms of breast cancer. An increasing number of researches suggest that compared to chemotherapy alone, Chinese herbal medicine combined with chemotherapy could increase effectiveness and reduce toxicity caused by chemotherapy. Emerging experimental research continuously demonstrated some of the components in XYS could stop breast cancer tumor cells from growing. However, the efficacy and safety of modified XYS combined with chemotherapy remain to be determined. Therefore, it is essential to evaluate the comparative effectiveness and safety of modified XYS combined with chemotherapy in-depth, thus providing clinicians and policymakers with evidence-based guidance and new treatment options. Objective: To comprehensively evaluate the efficacy and safety of modified XYS in conjunction with chemotherapy in treating breast cancer by conducting a meta-analysis. Methods: 8 databases were systemically searched until April 3, 2022, including Web of Science PubMed, EMBASE, Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang, Chinese Scientific Journals Database (VIP), and Chinese Biological Medical Database (CBM). Relevant randomized controlled trials (RCTs) comparing modified XYS in combination with chemotherapy versus chemotherapy alone were included. For the evaluation of methodological quality, Cochrane Collaboration was considered. Software Review Manager (version 5.4) was used for data analysis. Software STATA (version 15.0) was employed for sensitivity analysis and publication bias. Results: Altogether, 17 RCTs involving 1207 patients were investigated in the current review. The findings revealed that modified XYS combined with chemotherapy could lead to beneficial improvements compared to chemotherapy alone. More specifically, the combined therapy could enhance the short-term efficacy in the treatment of solid tumors (OR: 1.74; 95% CI 1.27 to 2.39; P = 0.0006; I Conclusion: The existing evidence suggests modified XYS combined with chemotherapy leads to beneficial improvements in the management of breast cancer, which may serve as a promising therapy for breast cancer in clinical practice. Given the limited number of high quality RCTs, more rigorous, scientific, double-blinded, large-scale, multi-center clinical trials are warranted further. Systematic review registration: https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022357860.
Language	English
Publishing date	2023-03-22
Publishing country	Switzerland
Document type	Systematic Review
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2023.1050337
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Article ; Online: Chlorothalonil exposure compromised mouse oocyte in vitro maturation through inducing oxidative stress and activating MAPK pathway.

Wang, Yong-Sheng / Yang, Sheng-Ji / Wan, Zi-Xuan / Shen, Ao / Ahmad, Muhammad Jamil / Chen, Ming-Yue / Huo, Li-Jun / Pan, Jun-Hua

Ecotoxicology and environmental safety

2024 Volume 273, Page(s) 116100

Abstract: Chlorothalonil (CTL) is widely used in agricultural production and antifoulant additive globally due to its broad spectrum and non-systemic properties, resulting in its widespread existence in foods, soil and water. Extensive evidence demonstrated that ... ...

Abstract	Chlorothalonil (CTL) is widely used in agricultural production and antifoulant additive globally due to its broad spectrum and non-systemic properties, resulting in its widespread existence in foods, soil and water. Extensive evidence demonstrated that exposure to CTL induced adverse effects on organisms and in particular its reproductive toxicity has been attracted public concern. However, the influences of CTL on oocyte maturation is mysterious so far. In this study, we documented the toxic effects of CTL on oocyte in vitro maturation and the related underlying mechanisms. Exposure to CTL caused continuous activation of spindle assembly checkpoints (SAC) which in turn compromised meiotic maturation in mouse oocyte, featured by the attenuation of polar body extrusion (PBE). Detection of cytoskeletal dynamics demonstrated that CTL exposure weakened the acetylation level of α-tubulin and impaired meiotic spindle apparatus, which was responsible for the aberrant state of SAC. Meanwhile, exposure to CTL damaged the function of mitochondria, inducing the decline of ATP content and the elevation of reactive oxygen species (ROS), which thereby induced early apoptosis and DNA damage in mouse oocytes. In addition, exposure to CTL caused the alteration of the level of histone H3 methylation, indicative of the harmful effects of CTL on epigenetic modifications in oocytes. Further, the CTL-induced oxidative stress activated mitogen-activated protein kinase (MAPK) pathway and injured the maturation of oocytes. In summary, exposure to CTL damaged mouse oocyte in vitro maturation via destroying spindle assembly, inducing oxidative stress and triggering MAPK pathway activation.
MeSH term(s)	Animals ; Mice ; In Vitro Oocyte Maturation Techniques ; Mitogen-Activated Protein Kinases/metabolism ; Oxidative Stress ; Oocytes/metabolism ; Reactive Oxygen Species/metabolism ; Apoptosis ; Nitriles
Chemical Substances	tetrachloroisophthalonitrile (J718M71A7A) ; Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; Reactive Oxygen Species ; Nitriles
Language	English
Publishing date	2024-02-16
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	436536-7
ISSN	1090-2414 ; 0147-6513
ISSN (online)	1090-2414
ISSN	0147-6513
DOI	10.1016/j.ecoenv.2024.116100
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Article: Integrated Single-Cell RNA-Sequencing Analysis of Gastric Cancer Identifies FABP1 as a Novel Prognostic Biomarker.

Yang, Fan / Gan, Lianfang / Pan, Junhua / Chen, Yaying / Zhang, Hong / Huang, Ling

Journal of oncology

2022 Volume 2022, Page(s) 4761403

Abstract: Gastric cancer (GC) is usually diagnosed in an advanced stage at the first visit due to the atypical clinical symptoms. The low surgical resection rate and chemotherapy sensitivity result in dismal survival. Therefore, it is urgent to develop novel ... ...

Abstract	Gastric cancer (GC) is usually diagnosed in an advanced stage at the first visit due to the atypical clinical symptoms. The low surgical resection rate and chemotherapy sensitivity result in dismal survival. Therefore, it is urgent to develop novel biomarkers with high sensitivity and specificity to accurately assess the prognosis of GC patients. In the present study, 3385 differentially expressed genes (DEGs) were obtained from the single-cell RNA sequencing data of GC specimens. Using the unsupervised dimensionality reduction, we further found 3 subsets of cells including gastric cells, plasmacytoid dendritic cells, and memory T cells. Based on the cell clustering, we explored the key regulatory genes for GC progression by pseudo-time analysis and functional enrichment analysis. According to the results, the significant differentially expressed fatty acid-binding protein 1 (FABP1) verified by pseudo-time analysis was identified as the hub gene of GC progression. FABP1 was shown to be closely related to the long-term survival and the age at diagnosis of patients with GC in analysis based on the TCGA (The Cancer Genome Atlas) database. To further verify the role of FABP1 in GC, we performed immunohistochemical (IHC) analysis using the GC tissue microarray and found that the expression level of FABP1 was higher in GC tissues than in the adjacent tissues. Moreover, GC patients with higher expression of FABP1 had a worse clinical outcome. In summary, our study revealed that FABP1 is a potential effective biomarker for the prognosis of GC, and high expression of FABP1 predicts unsatisfactory survival.
Language	English
Publishing date	2022-06-28
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2461349-6
ISSN	1687-8469 ; 1687-8450
ISSN (online)	1687-8469
ISSN	1687-8450
DOI	10.1155/2022/4761403
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Article: Utility of Machine Learning and Radiomics Based on Cavity for Predicting the Therapeutic Response of MDR-TB.

Lv, Xinna / Li, Ye / Cai, Botao / He, Wei / Wang, Ren / Chen, Minghui / Pan, Junhua / Hou, Dailun

Infection and drug resistance

2023 Volume 16, Page(s) 6893–6904

Abstract: Background: Sputum culture result at the sixth month is essential for predicting therapeutic response to longer multidrug-resistant tuberculosis (MDR-TB) regimens. This study aimed to construct a predictive model using cavity-based radiomics to predict ... ...

Abstract	Background: Sputum culture result at the sixth month is essential for predicting therapeutic response to longer multidrug-resistant tuberculosis (MDR-TB) regimens. This study aimed to construct a predictive model using cavity-based radiomics to predict sputum status at the sixth month for MDR-TB patients treated with longer regimens. Methods: This retrospective study recruited 315 MDR-TB patients treated with longer regimens from two centers (250 patients from center 1 and 65 patients from center 2), who were divided into persistently positive and conversion to negative sputum culture groups according to sputum results. Radiomics features were extracted based on the cavity, and a radiomics model was selected and established using a random forest classifier. The clinical characteristics and primary CT signs with significant differences were integrated to build a clinical model. A combined model was generated using the radiomics and clinical model. ROC curves, F1-score and DCA curves were used to assess the predictive performance of the models. Results: Twenty-eight radiomics features were selected to build a radiomics model for predicting the sputum status. The radiomics model achieved good performance, with AUCs of 0.892 and 0.839 in the training and testing cohort, respectively, which was similar to the performance of the combined model (0.913 and 0.815) and much higher than that of the clinical model (0.688 and 0.525) in the two cohorts. Conclusion: The cavity-based radiomics model has the potential to predict sputum culture status for MDR-TB patients receiving longer regimens, which could guide follow-up treatment effectively.
Language	English
Publishing date	2023-10-28
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2494856-1
ISSN	1178-6973
ISSN	1178-6973
DOI	10.2147/IDR.S435984
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Article ; Online: Comparison of the Diagnostic Performance of MeltPro and Next-Generation Sequencing in Determining Fluoroquinolone Resistance in Multidrug-Resistant Tuberculosis Isolates.

Hu, Yan / Chi, Yuqing / Feng, Xin / Yu, Fengping / Li, Haoran / Shang, Yuanyuan / Pan, Junhua / Pang, Yu

The Journal of molecular diagnostics : JMD

2023 Volume 25, Issue 6, Page(s) 342–351

Abstract: This study systematically investigated the performance of MeltPro and next-generation sequencing in the diagnosis of fluoroquinolone (FQ) resistance among multidrug-resistant tuberculosis patients and explored the relationship between nucleotide ... ...

Abstract	This study systematically investigated the performance of MeltPro and next-generation sequencing in the diagnosis of fluoroquinolone (FQ) resistance among multidrug-resistant tuberculosis patients and explored the relationship between nucleotide alteration and the level of phenotypic susceptibility to FQs. From March 2019 to June 2020, a feasibility and validation study with both MeltPro and next-generation sequencing was performed in 126 patients with multidrug-resistant tuberculosis. Using phenotypic drug susceptibility testing as the gold standard, 95.3% (82 of 86) of ofloxacin-resistant isolates were identified correctly by MeltPro. In addition, whole-genome sequencing was able to detect 83 phenotypically ofloxacin-resistant isolates. The isolates with an individual gyrB mutation outside the quinolone resistance-determining region (QRDR) had minimum inhibitory concentrations (MICs) of ≤2 μg/mL. Despite showing low MICs close to the breakpoint for isolates carrying only gyrA_Ala90Val, the combined mutation gyrB_Asp461Asn caused the ofloxacin MIC to be eight higher than that obtained in Mycobacterium tuberculosis (MTB) isolates with the Ala90Val mutation alone (median, 32 μg/mL; P = 0.038). Heteroresistance was observed in 12 of 88 isolates harboring mutations in the QRDRs. In conclusion, our data show that MeltPro and the whole-genome sequencing assay correctly can identify FQ resistance caused by mutations in the gyrA QRDR. The combined gyrB_Asp461Asn mutation may significantly decrease in vitro FQ susceptibility of MTB isolates with low-level-resistance-associated gyrA mutations.
MeSH term(s)	Humans ; Fluoroquinolones/pharmacology ; Mycobacterium tuberculosis ; Microbial Sensitivity Tests ; Drug Resistance, Bacterial/genetics ; DNA Gyrase/genetics ; Tuberculosis, Multidrug-Resistant/diagnosis ; Tuberculosis, Multidrug-Resistant/drug therapy ; Tuberculosis, Multidrug-Resistant/microbiology ; Ofloxacin/pharmacology ; Mutation ; High-Throughput Nucleotide Sequencing ; Antitubercular Agents/pharmacology
Chemical Substances	Fluoroquinolones ; DNA Gyrase (EC 5.99.1.3) ; Ofloxacin (A4P49JAZ9H) ; Antitubercular Agents
Language	English
Publishing date	2023-05-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2000060-1
ISSN	1943-7811 ; 1525-1578
ISSN (online)	1943-7811
ISSN	1525-1578
DOI	10.1016/j.jmoldx.2023.02.003
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Article ; Online: Cryo-EM Structure of Full-length HIV-1 Env Bound With the Fab of Antibody PG16.

Pan, Junhua / Peng, Hanqin / Chen, Bing / Harrison, Stephen C

Journal of molecular biology

2020 Volume 432, Issue 4, Page(s) 1158–1168

Abstract: The HIV-1 envelope protein (Env) is the target of neutralizing antibodies and the template for vaccine immunogen design. The dynamic conformational equilibrium of trimeric Env influences its antigenicity and potential immunogenicity. Antibodies that bind ...

Abstract	The HIV-1 envelope protein (Env) is the target of neutralizing antibodies and the template for vaccine immunogen design. The dynamic conformational equilibrium of trimeric Env influences its antigenicity and potential immunogenicity. Antibodies that bind at the trimer apex stabilize a "closed" conformation characteristic of the most difficult to neutralize isolates. A goal of vaccine development is therefore to mimic the closed conformation in a designed immunogen. A disulfide-stabilized, trimeric Env ectodomain-the "SOSIP" construct-has many of the relevant properties; it is also particularly suitable for structure determination. Some single-molecule studies have, however, suggested that the SOSIP trimer is not a good representation of Env on the surface of a virion or an infected cell. We isolated Env (fully cleaved to gp120 and gp41) from the surface of expressing cells using tagged, apex-binding Fab PG16 and determined the structure of the PG16-Env complex by cryo-EM to an overall resolution of 4.6 Å. Placing the only purification tag on the Fab ensured that the isolated Env was continuously stabilized in its closed, native conformation. The Env structure in this complex corresponds closely to the SOSIP structures determined by both x-ray crystallography and cryo-EM. Although the membrane-interacting elements are not resolved in our reconstruction, we can make inferences about the connection between ectodomain and membrane-proximal external region (MPER) by reference to the published cryo-tomography structure of an Env "spike" and the NMR structure of the MPER-transmembrane segment. We discuss these results in view of the conflicting interpretations in the literature.
MeSH term(s)	Antibodies, Neutralizing/chemistry ; Antibodies, Neutralizing/immunology ; Antibodies, Neutralizing/metabolism ; Cryoelectron Microscopy/methods ; HEK293 Cells ; HIV Envelope Protein gp160/chemistry ; HIV Envelope Protein gp160/immunology ; HIV Envelope Protein gp160/metabolism ; HIV-1/immunology ; HIV-1/ultrastructure ; Humans ; Protein Binding ; env Gene Products, Human Immunodeficiency Virus/chemistry ; env Gene Products, Human Immunodeficiency Virus/immunology ; env Gene Products, Human Immunodeficiency Virus/metabolism
Chemical Substances	Antibodies, Neutralizing ; HIV Envelope Protein gp160 ; env Gene Products, Human Immunodeficiency Virus
Language	English
Publishing date	2020-01-11
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2019.11.028
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Zs.A 867: Show issues

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Book ; Conference proceedings: Optical test and measurement technology and equipment

Pan, Junhua

: 3rd International Symposium on Advanced Optical Manufacturing and Testing Technologies; 8 - 12 July 2007, Chengdu, China; [AOMATT]

(Proceedings of SPIE ; 6723)

2007

Institution	International Symposium on Advanced Optical Manufacturing and Testing Technologies Zhongguo-Guangxue-Xuehui
Event/congress	AOMATT (3, 2007.07.08-12, Chengdu) ; International Symposium on Advanced Optical Manufacturing and Testing Technologies (3, 2007.07.08-12, Chengdu)
Author's details	sponsored by COS, the Chinese Optical Society (China) ... Junhua Pan ..., ed
Series title	Proceedings of SPIE ; 6723
Language	English
Size	Getr. Zählung [ca. 460 S.]
Publisher	SPIE
Publishing place	Bellingham, Wash
Document type	Book ; Conference proceedings
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Book ; Conference proceedings: Optical test and measurement technology and equipment

Pan, Junhua

: 3rd International Symposium on Advanced Optical Manufacturing and Testing Technologies; 8 - 12 July 2007, Chengdu, China; [AOMATT]

(Proceedings of SPIE ; 6723)

2007

Institution	International Symposium on Advanced Optical Manufacturing and Testing Technologies Zhongguo-Guangxue-Xuehui
Event/congress	AOMATT (3, 2007.07.08-12, Chengdu) ; International Symposium on Advanced Optical Manufacturing and Testing Technologies (3, 2007.07.08-12, Chengdu)
Author's details	sponsored by COS, the Chinese Optical Society (China) ... Junhua Pan ..., ed
Series title	Proceedings of SPIE ; 6723
Language	English
Size	Getr. Zählung [ca. 480 S.]
Publisher	SPIE
Publishing place	Bellingham, Wash
Document type	Book ; Conference proceedings
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Book ; Conference proceedings: Optical test and measurement technology and equipment

Pan, Junhua

: 3rd International Symposium on Advanced Optical Manufacturing and Testing Technologies; 8 - 12 July 2007, Chengdu, China; [AOMATT]

(Proceedings of SPIE ; 6723)

2007

Institution	International Symposium on Advanced Optical Manufacturing and Testing Technologies Zhongguo-Guangxue-Xuehui
Event/congress	AOMATT (3, 2007.07.08-12, Chengdu) ; International Symposium on Advanced Optical Manufacturing and Testing Technologies (3, 2007.07.08-12, Chengdu)
Author's details	sponsored by COS, the Chinese Optical Society (China) ... Junhua Pan ..., ed
Series title	Proceedings of SPIE ; 6723
Language	English
Size	Getr. Zählung [ca. 480 S.]
Publisher	SPIE
Publishing place	Bellingham, Wash
Document type	Book ; Conference proceedings
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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