Article ; Online: Exploring the mechanism of nonylphenol-induced ovarian developmental delay of manila clams, Ruditapes philippinarum: Applying RNAi to toxicological analysis.
2024 Volume 356, Page(s) 141905
Abstract: Nonylphenol (NP) contamination in the coastal environment of China poses ecological risks to aquatic organisms. However, the endocrine disruptive impacts of NP on bivalves, particularly on ovarian development, remain poorly understood. In this study, ... ...
| Abstract | Nonylphenol (NP) contamination in the coastal environment of China poses ecological risks to aquatic organisms. However, the endocrine disruptive impacts of NP on bivalves, particularly on ovarian development, remain poorly understood. In this study, Manila clams Ruditapes philippinarum at the developing stage of gonad were exposed to 1.0 μg/L NP for 21 days. Utilizing RNA interference (RNAi) to suppress ER gene expression, we observed a delay in ovarian development as evidenced by histological observations under both NP and NPRi (NP with ER-RNAi) treatment, with Vtg elevation exclusive to the NP group. Comprehensive analyses encompassing transcriptomics, real-time quantitative PCR, and steroid hormone measurement revealed significant alterations in aldosterone synthesis, estrogen signaling, and thyroid hormone synthesis. These pathways showed similar perturbations in both NP and NPRi groups compared to controls. Notably, the NPRi group exhibited distinct enrichment in PPAR and insulin signaling pathways, may implicating these in ER function suppression. Steroid hormone biosynthesis was notably reduced in both treatments, pointing to a profound impact on hormone synthesis. The contrast between in vivo and in vitro findings suggests that NP's detrimental effects on ovarian development may primarily involve neuroendocrine regulation of steroidogenesis. This investigation highlights the complex dynamics of NP-induced endocrine disruption in bivalves, emphasizing the pivotal role of ER and associated pathways. |
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| MeSH term(s) | Animals ; Phenols/toxicity ; Female ; Ovary/drug effects ; Ovary/metabolism ; Bivalvia/drug effects ; Bivalvia/genetics ; Endocrine Disruptors/toxicity ; Water Pollutants, Chemical/toxicity ; RNA Interference ; China ; Receptors, Estrogen/metabolism ; Receptors, Estrogen/genetics |
| Chemical Substances | Phenols ; nonylphenol (79F6A2ILP5) ; Endocrine Disruptors ; Water Pollutants, Chemical ; Receptors, Estrogen |
| Language | English |
| Publishing date | 2024-04-03 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 120089-6 |
| ISSN | 1879-1298 ; 0045-6535 ; 0366-7111 |
| ISSN (online) | 1879-1298 |
| ISSN | 0045-6535 ; 0366-7111 |
| DOI | 10.1016/j.chemosphere.2024.141905 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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