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  1. Article ; Online: Discussion on the need for correction during isotopic analysis of nitrogen by the denitrifier method.

    Hu, Jing / Pan, Mengyan / Li, Yuliang / Xing, Meng / Cao, Yunning / Yang, Kaili / Liu, Weiguo

    RSC advances

    2023  Volume 13, Issue 20, Page(s) 13834–13839

    Abstract: The nitrogen and oxygen isotopes of ... ...

    Abstract The nitrogen and oxygen isotopes of NO
    Language English
    Publishing date 2023-05-05
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d3ra00371j
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Development of a risk prediction nomogram for sarcopenia in hemodialysis patients.

    Cai, Genlian / Ying, Jinping / Pan, Mengyan / Lang, Xiabing / Yu, Weiping / Zhang, Qinqin

    BMC nephrology

    2022  Volume 23, Issue 1, Page(s) 319

    Abstract: Background: Sarcopenia is associated with various adverse outcomes in hemodialysis patients. However, current tools for assessing and diagnosing sarcopenia have limited applicability. In this study, we aimed to develop a simple and reliable nomogram to ... ...

    Abstract Background: Sarcopenia is associated with various adverse outcomes in hemodialysis patients. However, current tools for assessing and diagnosing sarcopenia have limited applicability. In this study, we aimed to develop a simple and reliable nomogram to predict the risk of sarcopenia in hemodialysis patients that could assist physicians identify high-risk patients early.
    Methods: A total of 615 patients undergoing hemodialysis at the First Affiliated Hospital College of Medicine Zhejiang University between March to June 2021 were included. They were randomly divided into either the development cohort (n = 369) or the validation cohort (n = 246). Multivariable logistic regression analysis was used to screen statistically significant variables for constructing the risk prediction nomogram for Sarcopenia. The line plots were drawn to evaluate the effectiveness of the nomogram in three aspects, namely differentiation, calibration, and clinical net benefit, and were further validated by the Bootstrap method.
    Results: The study finally included five clinical factors to construct the nomogram, including age, C-reactive protein, serum phosphorus, body mass index, and mid-upper arm muscle circumference, and constructed a nomogram. The area under the ROC curve of the line chart model was 0.869, with a sensitivity and specificity of 77% sensitivity and 83%, the Youden index was 0.60, and the internal verification C-statistic was 0.783.
    Conclusions: This study developed and validated a nomogram model to predict the risk of sarcopenia in hemodialysis patients, which can be used for early identification and timely intervention in high-risk groups.
    MeSH term(s) C-Reactive Protein ; Humans ; Nomograms ; Phosphorus ; Renal Dialysis/adverse effects ; Retrospective Studies ; Sarcopenia/diagnosis ; Sarcopenia/epidemiology
    Chemical Substances Phosphorus (27YLU75U4W) ; C-Reactive Protein (9007-41-4)
    Language English
    Publishing date 2022-09-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2041348-8
    ISSN 1471-2369 ; 1471-2369
    ISSN (online) 1471-2369
    ISSN 1471-2369
    DOI 10.1186/s12882-022-02942-0
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  3. Article ; Online: Molecular engineering of chitinase from Bacillus sp. DAU101 for enzymatic production of chitooligosaccharides.

    Pan, Mengyan / Li, Jianghua / Lv, Xueqin / Du, Guocheng / Liu, Long

    Enzyme and microbial technology

    2019  Volume 124, Page(s) 54–62

    Abstract: Enzymatic production of chitooligosaccharides has high value in medicine and other fields. However, low chitinase activity and yield of chitooligosaccharides limit the production and application. Herein, we used a series of molecular biology strategies ... ...

    Abstract Enzymatic production of chitooligosaccharides has high value in medicine and other fields. However, low chitinase activity and yield of chitooligosaccharides limit the production and application. Herein, we used a series of molecular biology strategies to increase the expression of chitinase in Bacillus subtilis WB600. Upon addition of the signal peptide NprB, Chisb was successfully secreted to the outside of the cell and extracellular expression level reached 35.54 U/mL. Furthermore, optimizing Ribosome Binding Sites (RBSs) with spacer sequences, and combining molecular docking technology with site-directed mutagenesis, the expression level and the specific activity of Chisb was further increased to 51.67 U/mL and 249.62 U/mg, respectively. When colloidal chitin was used as the substrate, the chitooligosaccharides detected by ion chromatography were (GlcNAc)
    MeSH term(s) Bacillus subtilis/enzymology ; Bacillus subtilis/genetics ; Bacillus subtilis/metabolism ; Binding Sites ; Chitin/analogs & derivatives ; Chitin/metabolism ; Chitinases/genetics ; Chitinases/isolation & purification ; Chitinases/metabolism ; Cloning, Molecular ; DNA, Ribosomal Spacer/genetics ; Gene Expression ; Hydrogen-Ion Concentration ; Molecular Docking Simulation ; Mutagenesis, Site-Directed ; Protein Sorting Signals/genetics ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Substrate Specificity ; Temperature
    Chemical Substances DNA, Ribosomal Spacer ; Protein Sorting Signals ; Recombinant Proteins ; oligochitosan ; Chitin (1398-61-4) ; Chitinases (EC 3.2.1.14)
    Language English
    Publishing date 2019-01-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 423729-8
    ISSN 1879-0909 ; 0141-0229
    ISSN (online) 1879-0909
    ISSN 0141-0229
    DOI 10.1016/j.enzmictec.2019.01.012
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  4. Article ; Online: Identification of nitrate sources in the Jing River using dual stable isotopes, Northwest China

    Hu, Jing / Pan, Mengyan / Han, Tianhao / Zhuang, Zhi / Cao, Yunning / Yang, Kaili / Li, Yuliang / Liu, Weiguo

    Environ Sci Pollut Res. 2021 Dec., v. 28, no. 48 p.68633-68641

    2021  

    Abstract: Nitrate (NO₃⁻) contamination has become a dominant international problem in the aquatic environment, so identifying the sources and transformations of NO₃⁻ is the basis for improving water quality. Since the Jing River is the largest tributary of the Wei ...

    Abstract Nitrate (NO₃⁻) contamination has become a dominant international problem in the aquatic environment, so identifying the sources and transformations of NO₃⁻ is the basis for improving water quality. Since the Jing River is the largest tributary of the Wei River, to understand its water quality, this study collected surface water samples from the Shaanxi section of the Jing River during the dry season. The potential sources of NO₃⁻ were analyzed by hydrochemical and bi-isotopic methods, and the SIAR model was used to estimate the proportional contribution of each source. Results indicated that NO₃⁻-N was the main form of inorganic nitrogen in this area, and the average total nitrogen content was 10.23 mg·L⁻¹, which showed that nitrogen pollution was highly serious; the transformation process of nitrogen in this study area was mainly nitrification; The results of Bayesian model showed that manure and sewage contributed to the most NO₃⁻ (64.39%) in the dry season, followed by soil nitrogen, which was 26.35%. These results help to adopt better nitrogen management measures to meet the national environmental quality standards for surface water.
    Keywords Bayesian theory ; aquatic environment ; dry season ; hydrochemistry ; models ; nitrates ; nitrification ; nitrogen ; pollution ; rivers ; sewage ; soil ; surface water ; total nitrogen ; water quality ; China
    Language English
    Dates of publication 2021-12
    Size p. 68633-68641.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-021-15380-6
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  5. Article ; Online: Identification of nitrate sources in the Jing River using dual stable isotopes, Northwest China.

    Hu, Jing / Pan, Mengyan / Han, Tianhao / Zhuang, Zhi / Cao, Yunning / Yang, Kaili / Li, Yuliang / Liu, Weiguo

    Environmental science and pollution research international

    2021  Volume 28, Issue 48, Page(s) 68633–68641

    Abstract: ... Nitrate ( ... ...

    Abstract Nitrate (NO
    MeSH term(s) Bayes Theorem ; China ; Environmental Monitoring ; Nitrates/analysis ; Nitrogen Isotopes/analysis ; Rivers ; Water Pollutants, Chemical/analysis
    Chemical Substances Nitrates ; Nitrogen Isotopes ; Water Pollutants, Chemical
    Language English
    Publishing date 2021-07-17
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-021-15380-6
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  6. Article: [Directed evolution of chitinase Chisb and biosynthesis of chitooligosaccharides].

    Pan, Mengyan / Xu, Xianhao / Liu, Yanfeng / Li, Jianghua / Lü, Xueqin / Du, Guocheng / Liu, Long

    Sheng wu gong cheng xue bao = Chinese journal of biotechnology

    2019  Volume 35, Issue 9, Page(s) 1787–1796

    Abstract: Chitinase has a wide industrial application prospect. For example, it can degrade shrimp shells, crab shells and other crustacean waste into high value-added chitooligosaccharides. However, the low catalytic efficiency of chitinase greatly limits the ... ...

    Abstract Chitinase has a wide industrial application prospect. For example, it can degrade shrimp shells, crab shells and other crustacean waste into high value-added chitooligosaccharides. However, the low catalytic efficiency of chitinase greatly limits the production of chitooligosaccharides. In previous study, the we expressed a chitinase Chisb with high catalytic efficiency and studied its enzymatic properties. In order to further improve the catalytic efficiency of Chisb, with R13NprB-C-SP-H as the parent, here error-prone PCR was used to construct random mutant library to conduct directed evolution of chitinase Chisb. Two mutants C43D and E336R were obtained with 96-well plate primary screening and shaker-screening, and their enzymatic properties were also studied. The optimum temperature of C43D and E336R was 55 °C, and the optimum pH of C43D was 5.0, while that of E336R was 9.0. The catalytic efficiency of C43D and E336R was 1.35 times and 1.57 times higher than that of control. The chitooligosaccharide concentration of E336R and C43D was 2.53 g/L and 2.06 g/L, improved by 2.84 times and 2.31 times compared with the control (0.89 g/L), respectively. In addition, the substrate conversion rate of mutants E336R and C43D was 84.3% and 68.7%, improved by 54.6% and 39% compared with the control (29.7%), respectively. In summary, the study indicates that random mutation introduced by error-prone PCR can effectively improve the catalytic efficiency of chitinase Chisb. The positive mutants with higher catalytic efficiency obtained in the above study and their enzymatic property analysis have important research significance and application value for the biosynthesis of chitooligosaccharides.
    MeSH term(s) Biocatalysis ; Chitin/analogs & derivatives ; Chitinases ; Hydrogen-Ion Concentration ; Polymerase Chain Reaction
    Chemical Substances oligochitosan ; Chitin (1398-61-4) ; Chitinases (EC 3.2.1.14)
    Language Chinese
    Publishing date 2019-08-24
    Publishing country China
    Document type Journal Article
    ZDB-ID 1042206-7
    ISSN 1000-3061 ; 1042-749X
    ISSN 1000-3061 ; 1042-749X
    DOI 10.13345/j.cjb.190069
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