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Article ; Online: Mechanism, Kinetics, and Potential of Mean Force of Evaporation of Water from Aqueous Sodium Chloride Solutions of Varying Concentrations.

Pandey, Prashant Kumar / Chandra, Amalendu

The journal of physical chemistry. B

2023 Volume 127, Issue 20, Page(s) 4602–4612

Abstract: The mechanism, kinetics, and potential of mean force of evaporation of water from aqueous NaCl solutions are investigated through both unbiased molecular dynamics simulations and also biased simulations using the umbrella sampling method. The results are ...

Abstract	The mechanism, kinetics, and potential of mean force of evaporation of water from aqueous NaCl solutions are investigated through both unbiased molecular dynamics simulations and also biased simulations using the umbrella sampling method. The results are obtained for aqueous solutions of three different NaCl concentrations ranging from 0.6 to 6.0 m and also for pure water. The rate of evaporation is found to decrease in the presence of ions. It is found that the process of evaporation of a surface water molecule from ionic solutions can be triggered through its collision with another water or chloride ion. Such collisions provide the additional kinetic energy that is required for evaporation. However, when the collision takes place with a Cl
Language	English
Publishing date	2023-05-10
Publishing country	United States
Document type	Journal Article
ISSN	1520-5207
ISSN (online)	1520-5207
DOI	10.1021/acs.jpcb.2c09004
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Article ; Online: Revolution of nanotechnology in food packaging: Harnessing electrospun zein nanofibers for improved preservation - A review.

Nanda, Alka / Pandey, Prashant / Rajinikanth, P S / Singh, Neetu

International journal of biological macromolecules

2024 Volume 260, Issue Pt 1, Page(s) 129416

Abstract: Zein, a protein-based biopolymer derived from corn, has garnered attention as a promising and eco-friendly choice for packaging food due to its favorable physical attributes. The introduction of electrospinning technology has significantly advanced the ... ...

Abstract	Zein, a protein-based biopolymer derived from corn, has garnered attention as a promising and eco-friendly choice for packaging food due to its favorable physical attributes. The introduction of electrospinning technology has significantly advanced the production of zein-based nanomaterials. This cutting-edge technique enables the creation of nanofibers with customizable structures, offering high surface area and adjustable mechanical and thermal attributes. Moreover, the electrospinning process allows for integrating various additives, such as antioxidants, antimicrobial agents, and flavoring compounds, into the zein nanofibers, enhancing their functionalities for food preservation. In this comprehensive review, the various electrospinning techniques employed for crafting zein-based nanofibers, and we delve into their enhanced properties. Furthermore, the review illuminates the potential applications of zein nanofibers in active and intelligent packaging materials by incorporating diverse constituents. Altogether, this review highlights the considerable prospects of zein-based nanocomposites in the realm of food packaging, offering sustainable and innovative solutions for food industry.
MeSH term(s)	Nanofibers/chemistry ; Food Packaging ; Zein/chemistry ; Nanotechnology/methods ; Nanocomposites
Chemical Substances	Zein (9010-66-6)
Language	English
Publishing date	2024-01-13
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.129416
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Article ; Online: Robust Prototypical Few-Shot Organ Segmentation With Regularized Neural-ODEs.

Pandey, Prashant / Chasmai, Mustafa / Sur, Tanuj / Lall, Brejesh

IEEE transactions on medical imaging

2023 Volume 42, Issue 9, Page(s) 2490–2501

Abstract: Despite the tremendous progress made by deep learning models in image semantic segmentation, they typically require large annotated examples, and increasing attention is being diverted to problem settings like Few-Shot Learning (FSL) where only a small ... ...

Abstract	Despite the tremendous progress made by deep learning models in image semantic segmentation, they typically require large annotated examples, and increasing attention is being diverted to problem settings like Few-Shot Learning (FSL) where only a small amount of annotation is needed for generalisation to novel classes. This is especially seen in medical domains where dense pixel-level annotations are expensive to obtain. In this paper, we propose Regularized Prototypical Neural Ordinary Differential Equation (R-PNODE), a method that leverages intrinsic properties of Neural-ODEs, assisted and enhanced by additional cluster and consistency losses to perform Few-Shot Segmentation (FSS) of organs. R-PNODE constrains support and query features from the same classes to lie closer in the representation space thereby improving the performance over the existing Convolutional Neural Network (CNN) based FSS methods. We further demonstrate that while many existing Deep CNN-based methods tend to be extremely vulnerable to adversarial attacks, R-PNODE exhibits increased adversarial robustness for a wide array of these attacks. We experiment with three publicly available multi-organ segmentation datasets in both in-domain and cross-domain FSS settings to demonstrate the efficacy of our method. In addition, we perform experiments with seven commonly used adversarial attacks in various settings to demonstrate R-PNODE's robustness. R-PNODE outperforms the baselines for FSS by significant margins and also shows superior performance for a wide array of attacks varying in intensity and design.
MeSH term(s)	Image Processing, Computer-Assisted/methods ; Neural Networks, Computer ; Semantics
Language	English
Publishing date	2023-08-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	622531-7
ISSN	1558-254X ; 0278-0062
ISSN (online)	1558-254X
ISSN	0278-0062
DOI	10.1109/TMI.2023.3258069
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Article: Antecedents and consequences of adopting CLTS among tribal communities to become open defecation free: case study on Indian Swachh Bharat Abhiyan

Adlakha, Amit / Pathak, Paritosh / Kumar, Anup / Pandey, Prashant

Environmental science and pollution research. 2022 June, v. 29, no. 30

2022

Abstract: The Swachh Bharat Mission undertaken by the Government of India (GoI) has been successful in accomplishing this objective within a short period thereby catalyzing governance following Sustainable Development Goals (SDGs). It is the responsibility of any ... ...

Abstract	The Swachh Bharat Mission undertaken by the Government of India (GoI) has been successful in accomplishing this objective within a short period thereby catalyzing governance following Sustainable Development Goals (SDGs). It is the responsibility of any democratic nation to ensure that its citizens have universal access to adequate and equitable sanitation. The key approach adapted to igniting a change in sanitation behavior rather than constructing toilets. An effort has been made in the study to affirm that even the marginalized sections of the society residing in tribal communities of West Bengal and Chhattisgarh have benefitted from this Government of India Mission and derived the required benefits. There is enough literature available to support the justification that civil participation holds the key for successful implementation of CLTS thereby making the communities open defecation free (ODF). It is therefore imperative to understand the behavioral transformation that takes place during the CLTS implementation. A hybrid model has been proposed in the study making use of the theory of reasoned action (TRA) and theory of planned behavior (TPB) to understand the pre-adoption and post-adoption behavior of residents. In present study, used model was empirically tested for several hypotheses. The finding reflects towards subjective norms and facilitating conditions as major determinants that ensure the continuation of intention for CLTS. They play a pivotal role in improving the health and hygiene conditions in the region and thus decrease the risk of spread of epidemic diseases.
Keywords	case studies ; defecation ; governance ; hygiene ; models ; pollution ; research ; risk ; sanitation ; society ; sustainable development ; India
Language	English
Dates of publication	2022-06
Size	p. 45698-45715.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-022-18854-3
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Article: A prospective observational study to evaluate effect of heat inactivation on ABO titers performed by column agglutination technology and conventional tube technique.

Pandey, Prashant / Setya, Divya / Ranjan, Shweta / Singh, Mukesh Kumar

Asian journal of transfusion science

2023 Volume 17, Issue 1, Page(s) 41–47

Abstract: Background and aims: When determining ABO antibody titers, immunoglobulin G (IgG) antibodies can be masked by immunoglobulin M (IgM) antibodies. Hence, the measurement of actual concentration of IgG requires methods like heat inactivation (HI) of plasma. ...

Abstract	Background and aims: When determining ABO antibody titers, immunoglobulin G (IgG) antibodies can be masked by immunoglobulin M (IgM) antibodies. Hence, the measurement of actual concentration of IgG requires methods like heat inactivation (HI) of plasma. This study was aimed at determining the effects of HI on IgM and IgG titers performed by conventional tube technique (CTT) and column agglutination technique (CAT). Materials and methods: This was a prospective, observational study conducted from October 2019 to March 2020. All consecutive A, B, and O group donors who gave consent for participation were included. All samples were consecutively tested by CTT and CAT, before and after HI (pCTT, pCAT). Results: A total of 300 donors were included. IgG titers were found to be more than IgM titers. For group O, IgG titer results were higher for both anti-A and anti-B compared to group A and B. For group A, B, and O, pretreatment results were higher than posttreatment IgG titer results. Median anti-A titers were similar to median anti-B titers across all categories. Median IgM and IgG titers were higher for group O individuals than nongroup O individuals. There was reduction in IgG and IgM titers after HI of plasma. One log reduction in median titers was observed when ABO titers were performed by CAT and CTT. Conclusion: There is one log difference between median antibody titers estimated using heat inactivated and nonheat inactivated plasma. The use of HI for ABO isoagglutinin titer estimation can be considered in low resource settings.
Language	English
Publishing date	2023-03-01
Publishing country	India
Document type	Journal Article
ZDB-ID	2267310-6
ISSN	1998-3565 ; 0973-6247
ISSN (online)	1998-3565
ISSN	0973-6247
DOI	10.4103/ajts.ajts_175_20
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Article ; Online: The Effect of COVID Convalescent Plasma on Hospital Stay: A Retrospective Observational Study from a Tertiary Care Hospital in North India.

Pandey, Prashant / Ranjan, Shweta / Setya, Divya / Kumari, Supriya / Mandal, Saikat

Recent advances in anti-infective drug discovery

2023 Volume 19, Issue 2, Page(s) 95–103

Abstract: Introduction: COVID convalescent plasma (CCP) has been used as standard of care in patients all over the world. CCP is plasma collected from recently infected and currently recovered COVID-19 patients, which contains antiviral antibodies that can be ... ...

Abstract	Introduction: COVID convalescent plasma (CCP) has been used as standard of care in patients all over the world. CCP is plasma collected from recently infected and currently recovered COVID-19 patients, which contains antiviral antibodies that can be used to treat patients with COVID-19. Several studies have shown a shorter hospital stay and lower mortality in patients treated with convalescent plasma in comparison with those not treated with it. Objectives: This study aims to determine the effect of COVID convalescent plasma (CCP) on the length of hospital stay in symptomatic patients and to determine outcome of the disease in patients who were administered CCP. Methods: This was a retrospective observational study done at a tertiary health care centre from July 2020 to May 2021, including patients who received CCP during the course of their stay in the hospital. Results: Among 257 participants, the patients with multiple comorbidities who were administered CCP had the longest average length of stay in the hospital which was 15 days, out of which, 92 (35.8%) patients were discharged while 9 (3.5%) patients died. Also, the maximum number of deaths was observed in those patients who had no associated comorbidity, being 11 (4.3%). It was observed that earlier administration of CCP in patients (< 5 days from symptom onset) was associated with a higher number of discharges as compared to deaths. Conclusion: Our study indicates that CCP may be efficient in treating COVID-19 patients if given in early course of the disease.
MeSH term(s)	Humans ; COVID-19/therapy ; SARS-CoV-2 ; Length of Stay ; Tertiary Care Centers ; Immunization, Passive/adverse effects ; COVID-19 Serotherapy
Language	English
Publishing date	2023-04-06
Publishing country	United Arab Emirates
Document type	Observational Study ; Journal Article
ISSN	2772-4352
ISSN (online)	2772-4352
DOI	10.2174/2772434418666230407133720
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Article ; Online: An assessment of clinical and laboratory impact in a resource constraint setting: Does Rh and Kell phenotyping of donor units complement type and screen method of compatibility testing?

Pandey, Prashant / Setya, Divya / Ranjan, Shweta / Singh, Mukesh Kumar

Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine

2023 Volume 30, Issue 2, Page(s) 219–227

Abstract: Background and aim: Despite knowing benefits of extended phenotyping, a vast majority feel that phenotype matched units add to the cost of blood banking. The purpose of this study was to discuss advantages and disadvantages of performing Rh Kell ... ...

Abstract	Background and aim: Despite knowing benefits of extended phenotyping, a vast majority feel that phenotype matched units add to the cost of blood banking. The purpose of this study was to discuss advantages and disadvantages of performing Rh Kell phenotyping in Indian scenario. Materials and methods: This was a prospective, observational study conducted at a tertiary healthcare center between July 2014 and February 2020. All consecutive whole blood donors and all consecutive patients whose samples were sent for Rh-Kell phenotyping were included for calculating antigen, phenotype and gene frequencies. For rate of alloimmunization in patients transfused with phenotype matched units, all patients who were given Rh-Kell phenotype matched transfusions were included in the prophylactic antigen matched (PAM) category and those who were given random units were included in the non-PAM category. Results: A total of 37,588 donors and 258 patients were included in the study for calculation of antigen, phenotype and gene frequencies. Percentage similarity of phenotypes between patient and donor populations was 33.8%. For rate of alloimmunization, results of a total of 31,991 patient samples revealed 0.94% prevalence of unexpected antibodies; highest against the Rh system. Three patients in the non-PAM category and one in the PAM category were alloimmunized during follow-up. Significant clinical and laboratory impact of phenotyping was observed in terms of reduced turnaround time and consumption of resources. Conclusion: Rh-Kell phenotyping of donors can prevent alloimmunization, reduce cost burden on the patient and the laboratory and help the laboratory personnel in smooth routine testing.
MeSH term(s)	Humans ; Isoantibodies ; Prospective Studies ; Blood Transfusion ; Blood Donors ; Blood Banking
Chemical Substances	Isoantibodies
Language	English
Publishing date	2023-01-03
Publishing country	France
Document type	Observational Study ; Journal Article
ZDB-ID	1204698-x
ISSN	1953-8022 ; 1246-7820
ISSN (online)	1953-8022
ISSN	1246-7820
DOI	10.1016/j.tracli.2022.12.001
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Article ; Online: Single-cell transcriptome analysis identifies novel biomarkers involved in major liver cancer subtypes.

Swain, Asish Kumar / Pandey, Prashant / Sera, Riddhi / Yadav, Pankaj

Functional & integrative genomics

2023 Volume 23, Issue 3, Page(s) 235

Abstract: Hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) are the two aggressive subtypes of liver cancer (LC). Immense cellular heterogeneity and cross-talk between cancer and healthy cells make it challenging to treat these cancer ... ...

Abstract	Hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) are the two aggressive subtypes of liver cancer (LC). Immense cellular heterogeneity and cross-talk between cancer and healthy cells make it challenging to treat these cancer subtypes. To address these challenges, the study aims to systematically characterize the tumor heterogeneity of LC subtypes using single-cell RNA sequencing (scRNA-seq) datasets. The study combined 51,927 single cells from HCC, ICC, and healthy scRNA-seq datasets. After integrating the datasets, cell groups with similar gene expression patterns are clustered and cluster annotation has been performed based on gene markers. Cell-cell communication analysis (CCA) was implemented to understand the cross-talk between various cell types. Further, differential gene expression analysis and enrichment analysis were carried out to identify unique molecular drivers associated with HCC and ICC. Our analysis identified T cells, hepatocytes, epithelial cells, and monocyte as the major cell types present in the tumor microenvironment. Among them, abundance of natural killer (NK) cells in HCC, epithelial cells, and hepatocytes in ICC was detected. CCA revealed key interaction between T cells to NK cells in HCC and smooth muscle cells to epithelial cells in the ICC. Additionally, SOX4 and DTHD1 are the top differentially expressed genes (DEGs) in HCC, while keratin and CCL4 are in ICC. Enrichment analysis of DEGs reveals major upregulated genes in HCC affect protein folding mechanism and in ICC alter pathways involved in cell adhesion. The findings suggest potential targets for the development of novel therapeutic strategies for the treatment of these two aggressive subtypes of LC.
MeSH term(s)	Humans ; Liver Neoplasms/genetics ; Carcinoma, Hepatocellular/genetics ; Single-Cell Gene Expression Analysis ; Biomarkers ; Myocytes, Smooth Muscle ; Tumor Microenvironment ; SOXC Transcription Factors
Chemical Substances	Biomarkers ; SOX4 protein, human ; SOXC Transcription Factors
Language	English
Publishing date	2023-07-13
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2014670-X
ISSN	1438-7948 ; 1438-793X
ISSN (online)	1438-7948
ISSN	1438-793X
DOI	10.1007/s10142-023-01156-3
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Article ; Online: Plastic waste management for sustainable environment: techniques and approaches.

Pandey, Prashant / Dhiman, Manisha / Kansal, Ankur / Subudhi, Sarada Prasannan

Waste disposal & sustainable energy

2023 , Page(s) 1–18

Abstract: Excessive exploitation, negligence, non-degradable nature, and physical and chemical properties of plastic waste have resulted in a massive pollution load into the environment. Consequently, plastic entres the food chain and can cause serious health ... ...

Abstract	Excessive exploitation, negligence, non-degradable nature, and physical and chemical properties of plastic waste have resulted in a massive pollution load into the environment. Consequently, plastic entres the food chain and can cause serious health issues in aquatic animals and humans. The present review summarizes currently reported techniques and approaches for the removal of plastic waste. Many techniques, such as adsorption, coagulation, photocatalysis, and microbial degradation, and approaches like reduction, reuse and recycling are potentially in trend and differ from each other in their efficiency and interaction mechanism. Moreover, substantial advantages and challenges associated with these techniques and approaches are highlighted to develop an understanding of the selection of possible ways for a sustainable future. Nevertheless, in addition to the reduction of plastic waste from the ecosystem, many alternative opportunities have also been explored to cash plastic waste. These fields include the synthesis of adsorbents for the removal of pollutants from aqueous and gaseous stream, their utility in clothing, waste to energy and fuel and in construction (road making). Substantial evidence can be observed in the reduction of plastic pollution from various ecosystems. In addition, it is important to develop an understanding of factors that need to be emphasized while considering alternative approaches and opportunities to cash plastic waste (like adsorbent, clothing, waste to energy and fuel). The thrust of this review is to provide readers with a comprehensive overview of the development status of techniques and approaches to overcome the global issue of plastic pollution and the outlook on the exploitation of this waste as resources.
Language	English
Publishing date	2023-03-06
Publishing country	Singapore
Document type	Journal Article ; Review
ZDB-ID	2951499-X
ISSN	2524-7891 ; 2524-7980
ISSN (online)	2524-7891
ISSN	2524-7980
DOI	10.1007/s42768-023-00134-6
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Article: Optimized methods for random and targeted mutagenesis in field pea (

Pandey, Prashant Kumar / Bhowmik, Pankaj / Kagale, Sateesh

Frontiers in plant science

2022 Volume 13, Page(s) 995542

Abstract: Field pea is an important pulse crop for its dense nutritional profile and contribution to sustainable agricultural practices. Recently, it has received extensive attention as a potential leading source of plant-based proteins. However, the adoption of ... ...

Abstract	Field pea is an important pulse crop for its dense nutritional profile and contribution to sustainable agricultural practices. Recently, it has received extensive attention as a potential leading source of plant-based proteins. However, the adoption of peas as a mainstream source of proteins is affected by a relatively moderate protein content, anti-nutritional factors and high levels of off-flavor components that reduce protein quality. Availability of genetic variation for desirable seed quality traits is the foundation for the sustainable development of pea varieties with improved protein content and quality. Mutagenesis has been an important tool in gene functional characterization studies and creating genetic variability for crop breeding. Large-scale mutagenesis of a crop using physical and chemical agents requires diligent selection of the mutagen and optimization of its dose to increase the frequency of mutations. In this study, we present detailed optimized protocols for physical and chemical mutagenesis of pea using gamma irradiation and ethyl methanesulfonate (EMS), respectively. Gamma radiation and EMS titration kill curves were established to identify optimal doses of the two mutagenic agents. Based on germination, survival rate and growth phenotypes, a gamma radiation dose of 225 Gy and EMS concentration of 5 mm were selected as optimal dosages for mutagenesis in field pea. The presented protocol has been modified from previously established mutagenesis protocols in other crop plants. Our results indicate that the optimal mutagen dosage is genotype dependent. CRISPR/Cas-based gene editing provides a precise and rapid method for targeted genetic manipulation in plants. With the recent success of gene editing in pea using CRISPR/Cas, this innovative technology is expected to become an integral component of the gene discovery and crop improvement toolkit in pea. Here, we describe an optimized methods for targeted mutagenesis of pea protoplasts, including mesophyll protoplast extraction, PEG-mediated transformation and gene editing of a LOX gene using CRISPR/Cas system. The general strategies and methods of mutagenesis described here provide an essential resource for mutation breeding and functional genomics studies in pea. These methods also provide a foundation for similar studies in other crops.
Language	English
Publishing date	2022-09-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2613694-6
ISSN	1664-462X
ISSN	1664-462X
DOI	10.3389/fpls.2022.995542
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