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  1. Article ; Online: Long Non-Coding RNAs in

    Kalem, Murat C / Panepinto, John C

    Frontiers in cellular and infection microbiology

    2022  Volume 12, Page(s) 858317

    Abstract: Long non-coding RNAs (lncRNAs) are highly expressed and can modulate multiple cellular processes including transcription, splicing, translation, and many diverse signaling events. LncRNAs can act as sponges for miRNAs, RNA and DNA binding proteins, ... ...

    Abstract Long non-coding RNAs (lncRNAs) are highly expressed and can modulate multiple cellular processes including transcription, splicing, translation, and many diverse signaling events. LncRNAs can act as sponges for miRNAs, RNA and DNA binding proteins, functioning as competitive endogenous RNAs. The contribution of lncRNAs to microbial pathogenesis is largely neglected in eukaryotic pathogens despite the abundance of RNA sequencing datasets encompassing conditions of stress, gene deletions and conditions that mimic the host environment. The human fungal pathogen
    MeSH term(s) Cryptococcus neoformans/genetics ; Cryptococcus neoformans/metabolism ; Humans ; MicroRNAs/genetics ; RNA, Long Noncoding/metabolism ; Sequence Analysis, RNA
    Chemical Substances MicroRNAs ; RNA, Long Noncoding
    Language English
    Publishing date 2022-03-17
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2022.858317
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Correction for Banerjee et al., "

    Banerjee, Dithi / Umland, Timothy C / Panepinto, John C

    mSphere

    2023  Volume 8, Issue 5, Page(s) e0040623

    Language English
    Publishing date 2023-09-26
    Publishing country United States
    Document type Journal Article ; Published Erratum
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/msphere.00406-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Contributions of Ccr4 and Gcn2 to the Translational Response of

    Knowles, Corey M / Goich, David / Bloom, Amanda L M / Kalem, Murat C / Panepinto, John C

    mBio

    2023  Volume 14, Issue 2, Page(s) e0019623

    Abstract: In response to the host environment, the human pathogen Cryptococcus neoformans must rapidly reprogram its translatome from one which promotes growth to one which is responsive to host stress. In this study, we investigate the two events which comprise ... ...

    Abstract In response to the host environment, the human pathogen Cryptococcus neoformans must rapidly reprogram its translatome from one which promotes growth to one which is responsive to host stress. In this study, we investigate the two events which comprise translatome reprogramming: the removal of abundant, pro-growth mRNAs from the translating pool, and the regulated entry of stress-responsive mRNAs into the translating pool. Removal of pro-growth mRNAs from the translating pool is controlled primarily by two regulatory mechanisms, repression of translation initiation via Gcn2, and decay mediated by Ccr4. We determined that translatome reprogramming in response to oxidative stress requires both Gcn2 and Ccr4, whereas the response to temperature requires only Ccr4. Additionally, we assessed ribosome collision in response to host-relevant stress and found that collided ribosomes accumulated during temperature stress but not during oxidative stress. The phosphorylation of eIF2α that occurred as a result of translational stress led us to investigate the induction of the integrated stress response (ISR). We found that eIF2α phosphorylation varied in response to the type and magnitude of stress, yet all tested conditions induced translation of the ISR transcription factor Gcn4. However, Gcn4 translation did not necessarily result in canonical Gcn4-dependent transcription. Finally, we define the ISR regulon in response to oxidative stress. In conclusion, this study begins to reveal the translational regulation in response to host-relevant stressors in an environmental fungus which is capable of adapting to the environment inside the human host.
    MeSH term(s) Humans ; Cryptococcus neoformans/metabolism ; Ribosomes/metabolism ; Phosphorylation ; Oxidative Stress ; Cryptococcosis/microbiology ; Transcription Factors/metabolism ; Saccharomyces cerevisiae/genetics ; Protein Biosynthesis ; Receptors, CCR4/genetics ; Receptors, CCR4/metabolism ; Ribonucleases/genetics ; Saccharomyces cerevisiae Proteins/genetics ; Protein Serine-Threonine Kinases/genetics
    Chemical Substances Transcription Factors ; CCR4 protein, human ; Receptors, CCR4 ; CCR4 protein, S cerevisiae (EC 3.1.-) ; Ribonucleases (EC 3.1.-) ; Saccharomyces cerevisiae Proteins ; GCN2 protein, S cerevisiae (EC 2.7.11.1) ; Protein Serine-Threonine Kinases (EC 2.7.11.1)
    Language English
    Publishing date 2023-04-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.00196-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Tools for Assessing Translation in

    Knowles, Corey M / McIntyre, Kelcy M / Panepinto, John C

    Journal of fungi (Basel, Switzerland)

    2021  Volume 7, Issue 3

    Abstract: Cryptococcus ... ...

    Abstract Cryptococcus neoformans
    Language English
    Publishing date 2021-02-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof7030159
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Glucan Unmasking Identifies Regulators of Temperature-Induced Translatome Reprogramming in C. neoformans.

    Bloom, Amanda L M / Goich, David / Knowles, Corey M / Panepinto, John C

    mSphere

    2021  Volume 6, Issue 1

    Abstract: The cell walls of fungi are critical for cellular structure and rigidity but also serve as a major communicator to alert the cell to the changing environment. In response to stresses encountered in human hosts, pathogenic fungi remodel their cell walls. ... ...

    Abstract The cell walls of fungi are critical for cellular structure and rigidity but also serve as a major communicator to alert the cell to the changing environment. In response to stresses encountered in human hosts, pathogenic fungi remodel their cell walls. Masking the β-1,3-glucan component of the cell wall is critical to escape detection by innate immune cells. We previously demonstrated that β-1,3-glucan is unmasked in response to host temperature stress when translatome reprogramming is defective in
    MeSH term(s) Cell Wall/chemistry ; Cryptococcus neoformans/genetics ; Cryptococcus neoformans/metabolism ; Cryptococcus neoformans/pathogenicity ; Fungal Proteins/genetics ; Gene Expression Regulation, Fungal ; Glucans/classification ; Glucans/metabolism ; Hot Temperature ; Mitogen-Activated Protein Kinases/metabolism ; Signal Transduction
    Chemical Substances Fungal Proteins ; Glucans ; Mitogen-Activated Protein Kinases (EC 2.7.11.24)
    Language English
    Publishing date 2021-02-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/mSphere.01281-20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: A Conserved Gcn2-Gcn4 Axis Links Methionine Utilization and the Oxidative Stress Response in

    Stovall, Anna K / Knowles, Corey M / Kalem, Murat C / Panepinto, John C

    Frontiers in fungal biology

    2021  Volume 2

    Abstract: The fungal ... ...

    Abstract The fungal pathogen
    Language English
    Publishing date 2021-03-22
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2673-6128
    ISSN (online) 2673-6128
    DOI 10.3389/ffunb.2021.640678
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Protein Kinases at the Intersection of Translation and Virulence.

    Leipheimer, Jay / Bloom, Amanda L M / Panepinto, John C

    Frontiers in cellular and infection microbiology

    2019  Volume 9, Page(s) 318

    Abstract: As free living organisms, fungi are challenged with a variety of environmental insults that threaten their cellular processes. In some cases, these challenges mimic conditions present within mammals, resulting in the accidental selection of virulence ... ...

    Abstract As free living organisms, fungi are challenged with a variety of environmental insults that threaten their cellular processes. In some cases, these challenges mimic conditions present within mammals, resulting in the accidental selection of virulence factors over evolutionary time. Be it within a host or the soil, fungi must contend with environmental challenges through the production of stress effector proteins while maintaining factors required for viability in any condition. Initiation and upkeep of this balancing act is mainly under the control of kinases that affect the propensity and selectivity of protein translation. This review will focus on kinases in pathogenic fungi that facilitate a virulence phenotype through translational control.
    MeSH term(s) Animals ; Fungi/growth & development ; Fungi/pathogenicity ; Host-Pathogen Interactions ; Mammals ; Protein Biosynthesis ; Protein Kinases/metabolism ; Virulence ; Virulence Factors/biosynthesis
    Chemical Substances Virulence Factors ; Protein Kinases (EC 2.7.-)
    Language English
    Publishing date 2019-09-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2019.00318
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Morphotype-specific effector functions of Cryptococcus neoformans PUM1.

    Kaur, Jan Naseer / Panepinto, John C

    Scientific reports

    2016  Volume 6, Page(s) 23638

    Abstract: The basidiomycete fungal pathogen Cryptococcus neoformans requires the PUF protein, Pum1, for hyphal morphogenesis during sexual development. In this study we found that Pum1 was auto-repressive under growth as yeast, but that auto-repression was ... ...

    Abstract The basidiomycete fungal pathogen Cryptococcus neoformans requires the PUF protein, Pum1, for hyphal morphogenesis during sexual development. In this study we found that Pum1 was auto-repressive under growth as yeast, but that auto-repression was relieved during filamentous growth through utilization of an alternative transcription start site driven by the master filamentation regulator Znf2. In addition, Pum1 was required to stabilize the ZNF2 mRNA through an indirect mechanism suggesting that Znf2 and Pum1 each positively regulate the expression of the other to achieve the filamentous morphotype required for sexual development in Cryptococcus.
    MeSH term(s) Cryptococcus neoformans/genetics ; Cryptococcus neoformans/growth & development ; Cryptococcus neoformans/metabolism ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Gene Expression Regulation, Fungal ; Hyphae/growth & development ; RNA Splice Sites ; RNA, Fungal/genetics ; RNA-Binding Proteins/metabolism ; Transcription, Genetic
    Chemical Substances Fungal Proteins ; RNA Splice Sites ; RNA, Fungal ; RNA-Binding Proteins
    Language English
    Publishing date 2016-03-24
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/srep23638
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Puf4 Mediates Post-transcriptional Regulation of Cell Wall Biosynthesis and Caspofungin Resistance in Cryptococcus neoformans.

    Kalem, Murat C / Subbiah, Harini / Leipheimer, Jay / Glazier, Virginia E / Panepinto, John C

    mBio

    2021  Volume 12, Issue 1

    Abstract: The human fungal ... ...

    Abstract The human fungal pathogen
    MeSH term(s) Antifungal Agents/pharmacology ; Caspofungin/pharmacology ; Cell Wall/drug effects ; Cell Wall/genetics ; Cell Wall/metabolism ; Chitin/metabolism ; Chitin Synthase/metabolism ; Cryptococcosis/microbiology ; Cryptococcus neoformans/drug effects ; Cryptococcus neoformans/genetics ; Drug Resistance, Fungal/genetics ; Drug Resistance, Fungal/physiology ; Echinocandins ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Glucans/metabolism ; Glucosyltransferases/genetics ; Glucosyltransferases/metabolism ; Humans ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism ; Saccharomyces cerevisiae Proteins ; beta-Glucans/metabolism
    Chemical Substances Antifungal Agents ; Echinocandins ; Fungal Proteins ; Glucans ; Membrane Proteins ; RNA-Binding Proteins ; Saccharomyces cerevisiae Proteins ; beta-Glucans ; Chitin (1398-61-4) ; beta-1,3-glucan (9051-97-2) ; Glucosyltransferases (EC 2.4.1.-) ; glucan synthase (EC 2.4.1.-) ; Chitin Synthase (EC 2.4.1.16) ; FKS1 protein, S cerevisiae (EC 2.4.1.34) ; Caspofungin (F0XDI6ZL63)
    Language English
    Publishing date 2021-01-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mBio.03225-20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Cryptococcus neoformans Coinfection Dampens the TNF-α Response in HIV-1-Infected Human THP-1 Macrophages.

    Kalem, Murat C / Humby, Monica S / Wohlfert, Elizabeth A / Jacobs, Amy / Panepinto, John C

    mSphere

    2021  Volume 6, Issue 2

    Abstract: Cryptococcus ... ...

    Abstract Cryptococcus neoformans
    MeSH term(s) Coinfection/immunology ; Coinfection/microbiology ; Coinfection/virology ; Cryptococcus neoformans/immunology ; Cryptococcus neoformans/pathogenicity ; HIV-1/immunology ; HIV-1/pathogenicity ; Humans ; Lung ; Macrophages/immunology ; Macrophages/virology ; Protein Serine-Threonine Kinases/metabolism ; Signal Transduction/immunology ; THP-1 Cells ; Tumor Necrosis Factor-alpha/analysis ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/immunology ; Up-Regulation ; NF-kappaB-Inducing Kinase
    Chemical Substances Tumor Necrosis Factor-alpha ; Protein Serine-Threonine Kinases (EC 2.7.11.1)
    Language English
    Publishing date 2021-03-24
    Publishing country United States
    Document type Journal Article
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/mSphere.00213-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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