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  1. Article ; Online: Amnion responses to intrauterine inflammation and effects of inhibition of TNF signaling in preterm Rhesus macaque

    Pietro Presicce / Monica Cappelletti / Marco Morselli / Feiyang Ma / Paranthaman Senthamaraikannan / Giulia Protti / Brian B. Nadel / Laila Aryan / Mansoureh Eghbali / Lukasz Salwinski / Neema Pithia / Emily De Franco / Lisa A. Miller / Matteo Pellegrini / Alan H. Jobe / Claire A. Chougnet / Suhas G. Kallapur

    iScience, Vol 26, Iss 11, Pp 108118- (2023)

    2023  

    Abstract: Summary: Intrauterine infection/inflammation (IUI) is a frequent complication of pregnancy leading to preterm labor and fetal inflammation. How inflammation is modulated at the maternal-fetal interface is unresolved. We compared transcriptomics of amnion ...

    Abstract Summary: Intrauterine infection/inflammation (IUI) is a frequent complication of pregnancy leading to preterm labor and fetal inflammation. How inflammation is modulated at the maternal-fetal interface is unresolved. We compared transcriptomics of amnion (a fetal tissue in contact with amniotic fluid) in a preterm Rhesus macaque model of IUI induced by lipopolysaccharide with human cohorts of chorioamnionitis. Bulk RNA sequencing (RNA-seq) amnion transcriptomic profiles were remarkably similar in both Rhesus and human subjects and revealed that induction of key labor-mediating genes such as IL1 and IL6 was dependent on nuclear factor κB (NF-κB) signaling and reversed by the anti-tumor necrosis factor (TNF) antibody Adalimumab. Inhibition of collagen biosynthesis by IUI was partially restored by Adalimumab. Interestingly, single-cell transcriptomics, flow cytometry, and immunohistology demonstrated that a subset of amnion mesenchymal cells (AMCs) increase CD14 and other myeloid cell markers during IUI both in the human and Rhesus macaque. Our data suggest that CD14+ AMCs represent activated AMCs at the maternal-fetal interface.
    Keywords Immunology ; Bioinformatics ; Omics ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2023-11-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: The induction of preterm labor in rhesus macaques is determined by the strength of immune response to intrauterine infection.

    Monica Cappelletti / Pietro Presicce / Ma Feiyang / Paranthaman Senthamaraikannan / Lisa A Miller / Matteo Pellegrini / Myung S Sim / Alan H Jobe / Senad Divanovic / Sing Sing Way / Claire A Chougnet / Suhas G Kallapur

    PLoS Biology, Vol 19, Iss 9, p e

    2021  Volume 3001385

    Abstract: Intrauterine infection/inflammation (IUI) is a major contributor to preterm labor (PTL). However, IUI does not invariably cause PTL. We hypothesized that quantitative and qualitative differences in immune response exist in subjects with or without PTL. ... ...

    Abstract Intrauterine infection/inflammation (IUI) is a major contributor to preterm labor (PTL). However, IUI does not invariably cause PTL. We hypothesized that quantitative and qualitative differences in immune response exist in subjects with or without PTL. To define the triggers for PTL, we developed rhesus macaque models of IUI driven by lipopolysaccharide (LPS) or live Escherichia coli. PTL did not occur in LPS challenged rhesus macaques, while E. coli-infected animals frequently delivered preterm. Although LPS and live E. coli both caused immune cell infiltration, E. coli-infected animals showed higher levels of inflammatory mediators, particularly interleukin 6 (IL-6) and prostaglandins, in the chorioamnion-decidua and amniotic fluid (AF). Neutrophil infiltration in the chorio-decidua was a common feature to both LPS and E. coli. However, neutrophilic infiltration and IL6 and PTGS2 expression in the amnion was specifically induced by live E. coli. RNA sequencing (RNA-seq) analysis of fetal membranes revealed that specific pathways involved in augmentation of inflammation including type I interferon (IFN) response, chemotaxis, sumoylation, and iron homeostasis were up-regulated in the E. coli group compared to the LPS group. Our data suggest that the intensity of the host immune response to IUI may determine susceptibility to PTL.
    Keywords Biology (General) ; QH301-705.5
    Subject code 570 ; 630
    Language English
    Publishing date 2021-09-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Intergeneric conjugation in Streptomyces peucetius and Streptomyces sp. strain C5: chromosomal integration and expression of recombinant plasmids carrying the chiC gene.

    Paranthaman, Senthamaraikannan / Dharmalingam, Kuppamuthu

    Applied and environmental microbiology

    2002  Volume 69, Issue 1, Page(s) 84–91

    Abstract: Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline ... ...

    Abstract Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. strain C5 were transformed using E. coli ET12567(pUZ8002) as a conjugal donor. When this donor species, carrying pSET152, was mated with Streptomyces strains, the resident plasmid was mobilized to the recipient and the transferred DNA was also integrated into the recipient chromosome. Analysis of the exconjugants showed stable integration of the plasmid at a single chromosomal site (attB) of the Streptomyces genome. The DNA sequence of the chromosomal integration site was determined and shown to be conserved. However, the core sequence, where the crossover presumably occurred in C5 and S. peucetius, is TTC. These results also showed that the phiC31 integrative recombination is active and the phage attP site is functional in S. peucetius as well as in C5. The efficiency and specificity of phiC31-mediated site-specific integration of the plasmid in the presence of a 3.7-kb homologous DNA sequence indicates that integrative recombination is preferred under these conditions. The integration of plasmid DNA did not affect antibiotic biosynthesis or biosynthesis of essential amino acids. Integration of a single copy of a mutant chiC into the wild-type S. peucetius chromosome led to the production of 30-fold more chitinase.
    MeSH term(s) Attachment Sites, Microbiological/genetics ; Bacterial Proteins ; Bacteriophages/genetics ; Base Sequence ; Chitinases/genetics ; Chromosomes, Bacterial/genetics ; Conjugation, Genetic ; Escherichia coli/genetics ; Molecular Sequence Data ; Plasmids/genetics ; Recombination, Genetic ; Sequence Analysis, DNA ; Streptomyces/classification ; Streptomyces/genetics ; Transformation, Bacterial
    Chemical Substances Bacterial Proteins ; Chitinases (EC 3.2.1.14) ; chitinase C-1 (EC 3.2.1.14)
    Language English
    Publishing date 2002-12-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.69.1.84-91.2003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  4. Article: ChiS histidine kinase negatively regulates the production of chitinase ChiC in Streptomyces peucetius

    Rabbind Singh, Amrathlal / Chitra Thangavel / Kuppamuthu Dharmalingam / Paranthaman Senthamaraikannan / Ravikanth Danda / Shunmugiah Karutha Pandian

    Microbiological research. 2014 Feb., Mar., v. 169

    2014  

    Abstract: Computational analysis of sequence homology of the chiSRC gene cluster, encoding a chitinase in Streptomyces peucetius, showed that the gene cluster could be a two-component regulon comprising a sensor kinase (chiS) and a response regulator (chiR). To ... ...

    Abstract Computational analysis of sequence homology of the chiSRC gene cluster, encoding a chitinase in Streptomyces peucetius, showed that the gene cluster could be a two-component regulon comprising a sensor kinase (chiS) and a response regulator (chiR). To prove that the ChiSRC is an authentic two-component system, the chiS gene was cloned and expressed in E.coli and the purified protein was used for biochemical analysis. In this report, we provide biochemical evidence to show that the sensor kinase encoded by chiS gene indeed is a histidine kinase capable of autophosphorylation and the histidine 144 residue of the ChiS protein is the phosphate acceptor. An insertion mutation at the chiS locus led to overproduction chitinase protein in S. peucetius implying that the chiC gene is negatively regulated by the two-component system.
    Keywords chitinase ; histidine ; histidine kinase ; loci ; molecular cloning ; multigene family ; mutation ; phosphates ; protein phosphorylation ; regulon ; sequence homology ; Streptomyces peucetius
    Language English
    Dates of publication 2014-03
    Size p. 155-162.
    Publishing place Elsevier GmbH
    Document type Article
    ZDB-ID 1189614-0
    ISSN 1618-0623 ; 0944-5013
    ISSN (online) 1618-0623
    ISSN 0944-5013
    DOI 10.1016/j.micres.2013.07.006
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  5. Article ; Online: Intraamniotic Zika virus inoculation of pregnant rhesus macaques produces fetal neurologic disease

    Lark L. Coffey / Rebekah I. Keesler / Patricia A. Pesavento / Kevin Woolard / Anil Singapuri / Jennifer Watanabe / Christina Cruzen / Kari L. Christe / Jodie Usachenko / JoAnn Yee / Victoria A. Heng / Eliza Bliss-Moreau / J. Rachel Reader / Wilhelm von Morgenland / Anne M. Gibbons / Kenneth Jackson / Amir Ardeshir / Holly Heimsath / Sallie Permar /
    Paranthaman Senthamaraikannan / Pietro Presicce / Suhas G. Kallapur / Jeffrey M. Linnen / Kui Gao / Robert Orr / Tracy MacGill / Michelle McClure / Richard McFarland / John H. Morrison / Koen K. A. Van Rompay

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 12

    Abstract: Zika virus infection of pregnant women can cause congenital brain defects. Here, Coffey et al. establish a pregnant rhesus macaque model, using intravenous and intraamniotic route of infection, that reliably reproduces fetal neurologic defects of ... ...

    Abstract Zika virus infection of pregnant women can cause congenital brain defects. Here, Coffey et al. establish a pregnant rhesus macaque model, using intravenous and intraamniotic route of infection, that reliably reproduces fetal neurologic defects of congenital Zika syndrome in humans.
    Keywords Science ; Q
    Language English
    Publishing date 2018-06-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Intraamniotic Zika virus inoculation of pregnant rhesus macaques produces fetal neurologic disease

    Lark L. Coffey / Rebekah I. Keesler / Patricia A. Pesavento / Kevin Woolard / Anil Singapuri / Jennifer Watanabe / Christina Cruzen / Kari L. Christe / Jodie Usachenko / JoAnn Yee / Victoria A. Heng / Eliza Bliss-Moreau / J. Rachel Reader / Wilhelm von Morgenland / Anne M. Gibbons / Kenneth Jackson / Amir Ardeshir / Holly Heimsath / Sallie Permar /
    Paranthaman Senthamaraikannan / Pietro Presicce / Suhas G. Kallapur / Jeffrey M. Linnen / Kui Gao / Robert Orr / Tracy MacGill / Michelle McClure / Richard McFarland / John H. Morrison / Koen K. A. Van Rompay

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 12

    Abstract: Zika virus infection of pregnant women can cause congenital brain defects. Here, Coffey et al. establish a pregnant rhesus macaque model, using intravenous and intraamniotic route of infection, that reliably reproduces fetal neurologic defects of ... ...

    Abstract Zika virus infection of pregnant women can cause congenital brain defects. Here, Coffey et al. establish a pregnant rhesus macaque model, using intravenous and intraamniotic route of infection, that reliably reproduces fetal neurologic defects of congenital Zika syndrome in humans.
    Keywords Science ; Q
    Language English
    Publishing date 2018-06-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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