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  1. Article ; Online: Enhanced Degradability, Mechanical Properties, and Flame Retardation of Poly(Lactic Acid) Composite with New Zealand Jade (Pounamu) Particles.

    Lin, Lilian / Dang, Quang A / Park, Heon E

    Polymers

    2023  Volume 15, Issue 15

    Abstract: Plastic pollution has become a global concern, demanding urgent attention and concerted efforts to mitigate its environmental impacts. Biodegradable plastics have emerged as a potential solution, offering the prospect of reduced harm through degradation ... ...

    Abstract Plastic pollution has become a global concern, demanding urgent attention and concerted efforts to mitigate its environmental impacts. Biodegradable plastics have emerged as a potential solution, offering the prospect of reduced harm through degradation over time. However, the lower mechanical strength and slower degradation process of biodegradable plastics have hindered their widespread adoption. In this study, we investigate the incorporation of New Zealand (NZ) jade (pounamu) particles into poly(lactic acid) (PLA) to enhance the performance of the resulting composite. We aim to improve mechanical strength, flame retardation, and degradability. The material properties and compatibility with 3D printing technology were examined through a series of characterization techniques, including X-ray diffraction, dispersive X-ray fluorescence spectrometry, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermogravimetric analysis, 3D printing, compression molding, pycnometry, rheometry, tensile tests, three-point bending, and flammability testing. Our findings demonstrate that the addition of NZ jade particles significantly affects the density, thermal stability, and mechanical properties of the composites. Compounding NZ jade shows two different changes in thermal stability. It reduces flammability suggesting potential flame-retardant properties, and it accelerates the thermal degradation process as observed from the thermogravimetric analysis and the inferred decrease in molecular weight through rheometry. Thus, the presence of jade particles can also have the potential to enhance biodegradation, although further research is needed to assess its impact. The mechanical properties differ between compression-molded and 3D-printed samples, with compression-molded composites exhibiting higher strength and stiffness. Increasing jade content in composites further enhances their mechanical performance. Th results of this study contribute to the development of sustainable solutions for plastic pollution, paving the way for innovative applications and a cleaner environment.
    Language English
    Publishing date 2023-08-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym15153270
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Ratiometric Fluorescence Sensing System for Lead Ions Based on Self-Assembly of Bioprobes Triggered by Specific Pb

    Mehta, Pramod Kumar / Lee, JaeYoon / Oh, Eun-Taex / Park, Heon Joo / Lee, Keun-Hyeung

    ACS applied materials & interfaces

    2023  

    Abstract: Lead is one of the most toxic substances. However, there are few ratiometric fluorescent probes for sensing ... ...

    Abstract Lead is one of the most toxic substances. However, there are few ratiometric fluorescent probes for sensing Pb
    Language English
    Publishing date 2023-03-08
    Publishing country United States
    Document type Journal Article
    ISSN 1944-8252
    ISSN (online) 1944-8252
    DOI 10.1021/acsami.3c00567
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Long-term efficacy of encapsulated xenogeneic islet transplantation: Impact of encapsulation techniques and donor genetic traits.

    Park, Heon-Seok / Lee, Eun Young / You, Young-Hye / Rhee, Marie / Kim, Jong-Min / Hwang, Seong-Soo / Lee, Poong-Yeon

    Journal of diabetes investigation

    2024  

    Abstract: Aims/introduction: To investigate the long-term efficacy of various encapsulated xenogeneic islet transplantation, and to explore the impact of different donor porcine genetic traits on islet transplantation outcomes.: Materials and methods: Donor ... ...

    Abstract Aims/introduction: To investigate the long-term efficacy of various encapsulated xenogeneic islet transplantation, and to explore the impact of different donor porcine genetic traits on islet transplantation outcomes.
    Materials and methods: Donor porcine islets were obtained from wild-type, α1,3-galactosyltransferase knockout (GTKO) and GTKO with overexpression of membrane cofactor protein genotype. Naked, alginate, alginate-chitosan (AC), alginate-perfluorodecalin (A-PFD) and AC-perfluorodecalin (AC-PFD) encapsulated porcine islets were transplanted into diabetic mice.
    Results: In vitro assessments showed no differences in the viability and function of islets across encapsulation types and donor porcine islet genotypes. Xenogeneic encapsulated islet transplantation with AC-PFD capsules showed the most favorable long-term outcomes, maintaining normal blood glucose levels for 180 days. A-PFD capsules showed comparable results to AC-PFD capsules, followed by AC capsules and alginate capsules. Conversely, blood glucose levels in naked islet transplantation increased to >300 mg/dL within a week after transplantation. Naked islet transplantation outcomes showed no improvement based on donor islet genotype. However, alginate or AC capsules showed delayed increases in blood glucose levels for GTKO and GTKO with overexpression of membrane cofactor protein porcine islets compared with wild-type porcine islets.
    Conclusion: The AC-PFD capsule, designed to ameliorate both hypoxia and inflammation, showed the highest long-term efficacy in xenogeneic islet transplantation. Genetic modifications of porcine islets with GTKO or GTKO with overexpression of membrane cofactor protein did not influence naked islet transplantation outcomes, but did delay graft failure when encapsulated.
    Language English
    Publishing date 2024-04-18
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2625840-7
    ISSN 2040-1124 ; 2040-1116
    ISSN (online) 2040-1124
    ISSN 2040-1116
    DOI 10.1111/jdi.14216
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A strategy to prevent atherosclerosis via TNF receptor regulation.

    Kim, Chan Woo / Oh, Eun-Taex / Park, Heon Joo

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    2021  Volume 35, Issue 3, Page(s) e21391

    Abstract: Atherosclerosis is a chronic inflammatory disease of the arterial wall. It has been known that development of atherosclerosis is closely related to activation of tumor necrosis factor α (TNF-α). The objective of this study was to elucidate the effects of ...

    Abstract Atherosclerosis is a chronic inflammatory disease of the arterial wall. It has been known that development of atherosclerosis is closely related to activation of tumor necrosis factor α (TNF-α). The objective of this study was to elucidate the effects of TNF-α blockade with brusatol on endothelial activation under pro-atherosclerotic conditions. To this end, we examined the effects of brusatol on TNF-α-induced intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in human aortic endothelial cells (HAECs) using western blot and THP-1 adhesion assays. Brusatol induced a decrease in TNF-α-induced ICAM-1 and VCAM-1 expression through inhibiting TNFR1 expression, leading to suppression of endothelial inflammation independently of the NRF2 (nuclear factor erythroid 2-related factor 2) pathway. The mechanism underlying brusatol-induced TNF receptor 1 (TNFR1) inhibition was investigated with the aid of protein synthesis, co-immunoprecipitation, and cytokine arrays. Notably, brusatol inhibited TNFR1 protein synthesis and suppressed both the canonical nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) signaling pathway and TNF-α-induced cytokine secretion. We further tested the functional effect of brusatol on atherosclerosis development in vivo using two different atherosclerosis mouse models, specifically, acute partial carotid ligation and conventional chronic high-fat diet-fed mouse models. Administration of brusatol led to significant suppression of atherosclerosis development in both mouse models. Our finding that brusatol prevents atherosclerosis via inhibition of TNFR1 protein synthesis supports the potential of downregulation of cell surface TNFR1 as an effective therapeutic approach to inhibit development of atherosclerosis.
    MeSH term(s) Animals ; Atherosclerosis/prevention & control ; Cells, Cultured ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Humans ; Intercellular Adhesion Molecule-1/genetics ; Male ; Mice ; Mice, Inbred C57BL ; Proteasome Endopeptidase Complex/physiology ; Quassins/therapeutic use ; Receptors, Tumor Necrosis Factor, Type I/antagonists & inhibitors ; Tumor Necrosis Factor-alpha/antagonists & inhibitors ; Vascular Cell Adhesion Molecule-1/genetics
    Chemical Substances Quassins ; Receptors, Tumor Necrosis Factor, Type I ; Tumor Necrosis Factor-alpha ; Vascular Cell Adhesion Molecule-1 ; Intercellular Adhesion Molecule-1 (126547-89-5) ; brusatol (14907-98-3) ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
    Language English
    Publishing date 2021-02-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639186-2
    ISSN 1530-6860 ; 0892-6638
    ISSN (online) 1530-6860
    ISSN 0892-6638
    DOI 10.1096/fj.202000764R
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Outcomes of Arthroscopic Scaphoid Excision and Lunocapitate Fusion for Advanced Traumatic Arthritis of the Wrist.

    Oh, Won-Taek / Park, Heon-Jung / Koh, Il-Hyun / Choi, Yun-Rak

    Clinics in orthopedic surgery

    2022  Volume 15, Issue 2, Page(s) 308–317

    Abstract: Background: Arthroscopic lunocapitate (LC) fusion can be an alternative surgical treatment for scapholunate advanced collapse (SLAC) or scaphoid nonunion advanced collapse (SNAC) of the wrist. We retrospectively reviewed patients who had arthroscopic LC ...

    Abstract Background: Arthroscopic lunocapitate (LC) fusion can be an alternative surgical treatment for scapholunate advanced collapse (SLAC) or scaphoid nonunion advanced collapse (SNAC) of the wrist. We retrospectively reviewed patients who had arthroscopic LC fusion to estimate clinical and radiological outcomes.
    Methods: From January 2013 to February 2017, all patients with SLAC (stage II or III) or SNAC (stage II or III) wrists, who underwent arthroscopic LC fusion with scaphoidectomy and were followed up for a minimum of 2 years, were enrolled in this retrospective study. Clinical outcomes included visual analog scale (VAS) pain, grip strength, active range of wrist motion, Mayo wrist score (MWS), and the Disabilities of Arm, Shoulder and Hand (DASH) score. Radiologic outcomes included bony union, carpal height ratio, joint space height ratio, and loosening of screws. We also performed group analysis between patients with 1 and 2 headless compression screws to fix the LC interval.
    Results: Eleven patients were assessed for 32.6 ± 8.0 months. Union was achieved in 10 patients (union rate, 90.9%). There was improvmenet in mean VAS pain score (from 7.9 ± 1.0 to 1.6 ± 0.7,
    Conclusions: Arthroscopic scaphoid excision and LC fusion for patients with advanced SLAC or SNAC of the wrist was effective and safe only in cases fixed with 2 headless compression screws. We recommend arthroscopic LC fusion using 2 screws rather than 1 to decrease radiolucent loosening, which might affect complications such as nonunion, delayed union, or screw migration.
    MeSH term(s) Humans ; Wrist ; Retrospective Studies ; Arthrodesis ; Scaphoid Bone/diagnostic imaging ; Scaphoid Bone/surgery ; Wrist Joint/diagnostic imaging ; Wrist Joint/surgery ; Arthritis ; Pain ; Range of Motion, Articular ; Treatment Outcome
    Language English
    Publishing date 2022-09-28
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2502788-8
    ISSN 2005-4408 ; 2005-291X
    ISSN (online) 2005-4408
    ISSN 2005-291X
    DOI 10.4055/cios22066
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Peripheral Nerve Blockade in Total Hip Arthroplasty: A Retrospective Study with Propensity Score Matching.

    Kwon, Hyuck Min / Lee, Tae Sung / Park, Heon Jung / Lee, Bora / Choi, Yong Seon / Park, Kwan Kyu

    Journal of clinical medicine

    2023  Volume 12, Issue 17

    Abstract: The effect of peripheral nerve block (PNB) according to leg lengthening following total hip arthroplasty (THA) has not been studied yet. The purpose of this study was to investigate the effect of PNB according to the change in leg length after THA. From ... ...

    Abstract The effect of peripheral nerve block (PNB) according to leg lengthening following total hip arthroplasty (THA) has not been studied yet. The purpose of this study was to investigate the effect of PNB according to the change in leg length after THA. From January 2016 to August 2021, 353 patients who underwent unilateral THA for osteonecrosis of the femoral head or osteoarthritis of the hip joint were retrospectively reviewed. The patients were divided into two groups for comparison: 217 patients who controlled postoperative pain using only intravenous venous patient-controlled analgesia (IV PCA) (PCA group) and 136 patients who controlled postoperative pain using PNB and IV PCA (PCA + PNB group). We further divided the patients into two groups (leg lengthening after surgery < 10 mm and >10 mm) and compared them. After propensity score matching, the PCA and PCA + PNB groups, with 134 patients each, were compared and analyzed. The pain intensity at rest was significantly lower in the PCA + PNB group compared with that in the PCA group at postoperative 6, 24, and 48 h (
    Language English
    Publishing date 2023-08-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm12175514
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: NQO1 regulates cell cycle progression at the G2/M phase.

    Oh, Eun-Taex / Kim, Ha Gyeong / Kim, Chul Hoon / Lee, Jeonghun / Kim, Chulhee / Lee, Jae-Seon / Cho, Yunmi / Park, Heon Joo

    Theranostics

    2023  Volume 13, Issue 3, Page(s) 873–895

    Abstract: Rationale: ...

    Abstract Rationale:
    MeSH term(s) Humans ; Cell Cycle ; Cell Division ; Cell Line, Tumor ; G2 Phase ; NAD(P)H Dehydrogenase (Quinone)/genetics ; NAD(P)H Dehydrogenase (Quinone)/metabolism ; Proto-Oncogene Proteins c-fos/metabolism ; Neoplasms/genetics
    Chemical Substances NAD(P)H Dehydrogenase (Quinone) (EC 1.6.5.2) ; NQO1 protein, human (EC 1.6.5.2) ; Proto-Oncogene Proteins c-fos ; CKS1B protein, human
    Language English
    Publishing date 2023-01-10
    Publishing country Australia
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592097-2
    ISSN 1838-7640 ; 1838-7640
    ISSN (online) 1838-7640
    ISSN 1838-7640
    DOI 10.7150/thno.77444
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: TRIM22 induces cellular senescence by targeting PHLPP2 in hepatocellular carcinoma.

    Kang, Donghee / Hwang, Hyun Jung / Baek, Yurim / Sung, Jee Young / Kim, KyeongJin / Park, Heon Joo / Ko, Young-Gyu / Kim, Yong-Nyun / Lee, Jae-Seon

    Cell death & disease

    2024  Volume 15, Issue 1, Page(s) 26

    Abstract: The ubiquitin-proteasome system is a vital protein degradation system that is involved in various cellular processes, such as cell cycle progression, apoptosis, and differentiation. Dysregulation of this system has been implicated in numerous diseases, ... ...

    Abstract The ubiquitin-proteasome system is a vital protein degradation system that is involved in various cellular processes, such as cell cycle progression, apoptosis, and differentiation. Dysregulation of this system has been implicated in numerous diseases, including cancer, vascular disease, and neurodegenerative disorders. Induction of cellular senescence in hepatocellular carcinoma (HCC) is a potential anticancer strategy, but the precise role of the ubiquitin-proteasome system in cellular senescence remains unclear. In this study, we show that the E3 ubiquitin ligase, TRIM22, plays a critical role in the cellular senescence of HCC cells. TRIM22 expression is transcriptionally upregulated by p53 in HCC cells experiencing ionizing radiation (IR)-induced senescence. Overexpression of TRIM22 triggers cellular senescence by targeting the AKT phosphatase, PHLPP2. Mechanistically, the SPRY domain of TRIM22 directly associates with the C-terminal domain of PHLPP2, which contains phosphorylation sites that are subject to IKKβ-mediated phosphorylation. The TRIM22-mediated PHLPP2 degradation leads to activation of AKT-p53-p21 signaling, ultimately resulting in cellular senescence. In both human HCC databases and patient specimens, the levels of TRIM22 and PHLPP2 show inverse correlations at the mRNA and protein levels. Collectively, our findings reveal that TRIM22 regulates cancer cell senescence by modulating the proteasomal degradation of PHLPP2 in HCC cells, suggesting that TRIM22 could potentially serve as a therapeutic target for treating cancer.
    MeSH term(s) Humans ; Carcinoma, Hepatocellular/genetics ; Proteasome Endopeptidase Complex ; Proto-Oncogene Proteins c-akt ; Tumor Suppressor Protein p53/genetics ; Liver Neoplasms/genetics ; Cellular Senescence/genetics ; Ubiquitins ; Tripartite Motif Proteins/genetics ; Repressor Proteins ; Minor Histocompatibility Antigens ; Phosphoprotein Phosphatases/genetics
    Chemical Substances Proteasome Endopeptidase Complex (EC 3.4.25.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Tumor Suppressor Protein p53 ; Ubiquitins ; TRIM22 protein, human ; Tripartite Motif Proteins ; Repressor Proteins ; Minor Histocompatibility Antigens ; PHLPP2 protein, human (EC 3.1.3.16) ; Phosphoprotein Phosphatases (EC 3.1.3.16)
    Language English
    Publishing date 2024-01-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2541626-1
    ISSN 2041-4889 ; 2041-4889
    ISSN (online) 2041-4889
    ISSN 2041-4889
    DOI 10.1038/s41419-024-06427-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Risk Factors and Characteristics of In-Hospital Falls After Spine Surgery: A Retrospective, Single-Center Cohort Study in the Republic of Korea.

    Shin, Jae-Won / Park, Heon Jung / Park, Yung / Ha, Joong-Won / Hong, Jung Hwa / Kim, Hak-Sun / Suk, Kyung-Soo / Moon, Seong-Hwan / Park, Si-Young / Lee, Byung-Ho / Kwon, Ji-Won

    JB & JS open access

    2024  Volume 9, Issue 2

    Abstract: Background: Falls after orthopaedic surgery can cause serious injuries, which lengthen hospital stays and increase medical expenses. This has prompted hospitals to implement various fall-prevention protocols. The aims of this study were to determine the ...

    Abstract Background: Falls after orthopaedic surgery can cause serious injuries, which lengthen hospital stays and increase medical expenses. This has prompted hospitals to implement various fall-prevention protocols. The aims of this study were to determine the incidence of in-hospital falls after spine surgery, to analyze the overall risk factors, to discern factors that have a major influence on falls, and to evaluate the effectiveness of the fall-prevention protocol that we implemented.
    Methods: This was a retrospective, single-center study including patients who underwent spine surgery from January 2011 to November 2021 at the National Health Insurance Service Ilsan Hospital (NHISIH) in Goyang, Republic of Korea. Reported falls among these patients were examined. Patient demographics; surgery type, date, and diagnosis; and fall date and time were evaluated.
    Results: Overall, 5,317 spine surgeries were performed, and 128 in-hospital falls were reported (overall incidence: 2.31%). From the multivariable analyses, older age and American Society of Anesthesiologists (ASA) score were identified as independent risk factors for in-hospital patient falls (multivariable adjusted hazard ratio [aHR] for age 70 to 79 years, 1.021 [95% confidence interval (CI), 1.01 to 1.031]; for age ≥80 years, 1.035 [1.01 to 1.06]; and for ASA score of 3, 1.02 [1.01 to 1.031]). Similar results were seen in the subgroup who underwent primary surgery. Within 2 weeks following surgery, the highest frequency of falls occurred at 3 to 7 days postoperatively. The lowest fall rate was observed in the evening (6 to 10 p.m.). Morbidities, including rib, spine, and extremity fractures, were recorded for 14 patients, but none of these patients underwent operative treatment related to the fall. The NHISIH implemented a comprehensive nursing care service in May 2015 and a fall protocol in May 2017, but the annual incidence rate did not improve. The fall rate was higher after thoracolumbar surgeries (2.47%) than after cervical surgeries (1.20%). Moreover, a higher fall rate was observed in thoracolumbar cases with a greater number of fusion levels and revision spine surgeries.
    Conclusions: Patients with advanced age, more comorbidities, a greater number of fusion levels, and revision surgeries and who are female are more vulnerable to in-hospital falls after spine surgery. Novel strategies that target these risk factors are warranted.
    Level of evidence: Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
    Language English
    Publishing date 2024-04-04
    Publishing country United States
    Document type Journal Article
    ISSN 2472-7245
    ISSN (online) 2472-7245
    DOI 10.2106/JBJS.OA.23.00096
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: FLRT2 prevents endothelial cell senescence and vascular aging by regulating the ITGB4/mTORC2/p53 signaling pathway.

    Hwang, Hyun Jung / Kang, Donghee / Kim, Jae-Ryong / Choi, Joon Hyuk / Ryu, Ji-Kan / Herman, Allison B / Ko, Young-Gyu / Park, Heon Joo / Gorospe, Myriam / Lee, Jae-Seon

    JCI insight

    2024  Volume 9, Issue 7

    Abstract: The roles of fibronectin leucine-rich transmembrane protein 2 (FLRT2) in physiological and pathological processes are not well known. Here, we identify a potentially novel function of FLRT2 in preventing endothelial cell senescence and vascular aging. We ...

    Abstract The roles of fibronectin leucine-rich transmembrane protein 2 (FLRT2) in physiological and pathological processes are not well known. Here, we identify a potentially novel function of FLRT2 in preventing endothelial cell senescence and vascular aging. We found that FLRT2 expression was lower in cultured senescent endothelial cells as well as in aged rat and human vascular tissues. FLRT2 mediated endothelial cell senescence via the mTOR complex 2, AKT, and p53 signaling pathway in human endothelial cells. We uncovered that FLRT2 directly associated with integrin subunit beta 4 (ITGB4) and thereby promoted ITGB4 phosphorylation, while inhibition of ITGB4 substantially mitigated the induction of senescence triggered by FLRT2 depletion. Importantly, FLRT2 silencing in mice promoted vascular aging, and overexpression of FLRT2 rescued a premature vascular aging phenotype. Therefore, we propose that FLRT2 could be targeted therapeutically to prevent senescence-associated vascular aging.
    MeSH term(s) Animals ; Humans ; Mice ; Rats ; Aging ; Endothelial Cells/metabolism ; Integrin beta4/genetics ; Integrin beta4/metabolism ; Mechanistic Target of Rapamycin Complex 2/metabolism ; Membrane Glycoproteins/metabolism ; Signal Transduction ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism
    Chemical Substances FLRT2 protein, mouse ; Integrin beta4 ; ITGB4 protein, human ; Mechanistic Target of Rapamycin Complex 2 (EC 2.7.11.1) ; Membrane Glycoproteins ; Tumor Suppressor Protein p53 ; FLRT2 protein, human
    Language English
    Publishing date 2024-04-08
    Publishing country United States
    Document type Journal Article
    ISSN 2379-3708
    ISSN (online) 2379-3708
    DOI 10.1172/jci.insight.172678
    Database MEDical Literature Analysis and Retrieval System OnLINE

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