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Article: Recent Advances in Endothelial Colony Forming Cells Toward Their Use in Clinical Translation.

Paschalaki, Koralia E / Randi, Anna M

2018 Volume 5, Page(s) 295

Abstract: The term "Endothelial progenitor cell" (EPC) has been used to describe multiple cell populations that express endothelial surface makers and promote vascularisation. However, the only population that has all the characteristics of a real "EPC" is the ... ...

Abstract	The term "Endothelial progenitor cell" (EPC) has been used to describe multiple cell populations that express endothelial surface makers and promote vascularisation. However, the only population that has all the characteristics of a real "EPC" is the Endothelial Colony Forming Cells (ECFC). ECFC possess clonal proliferative potential, display endothelial and not myeloid cell surface markers, and exhibit pronounced postnatal vascularisation ability
Language	English
Publishing date	2018-10-23
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2018.00295
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Article ; Online: Identification of coronavirus particles by electron microscopy: a complementary tool for deciphering COVID-19.

Havaki, Sophia / Evangelou, Konstantinos / Paschalaki, Koralia / Petty, Russell / Barnes, Peter J / Gorgoulis, Vassilis G

The European respiratory journal

2022

Language	English
Publishing date	2022-04-29
Publishing country	England
Document type	Letter
ZDB-ID	639359-7
ISSN	1399-3003 ; 0903-1936
ISSN (online)	1399-3003
ISSN	0903-1936
DOI	10.1183/13993003.00754-2022
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Article ; Online: Results of an international survey about methods used to isolate human endothelial colony-forming cells: guidance from the SSC on Vascular Biology of the ISTH.

Blandinières, Adeline / Randi, Anna M / Paschalaki, Koralia E / Guerin, Coralie L / Melero-Martin, Juan M / Smadja, David M

Journal of thrombosis and haemostasis : JTH

2023 Volume 21, Issue 9, Page(s) 2611–2619

Abstract: Background: Assessment of endothelial colony-forming cell (ECFC) number and vasculogenic properties is crucial for exploring vascular diseases and regeneration strategies. A previous survey of the Scientific and Standardization Committee on Vascular ... ...

Abstract	Background: Assessment of endothelial colony-forming cell (ECFC) number and vasculogenic properties is crucial for exploring vascular diseases and regeneration strategies. A previous survey of the Scientific and Standardization Committee on Vascular Biology of the International Society on Thrombosis and Haemostasis clarified key methodological points but highlighted a lack of standardization associated with ECFC culture. Objectives: The aim of this study was to provide expert consensus guidance on ECFC isolation and culture. Methods: We surveyed 21 experts from 10 different countries using a questionnaire proposed during the 2019 International Society on Thrombosis and Haemostasis Congress in Melbourne (Australia) to attain a consensus on ECFC isolation and culture. Results: We report here the consolidated results of the questionnaire. There was agreement on several general statements, mainly the technical aspects of ECFC isolation and cell culture. In contrast, on the points concerning the definition of a colony of ECFCs, the quantification of ECFCs, and the estimation of their age (in days or number of passages), the expert opinions were widely dispersed. Conclusion: Our survey clearly indicates an unmet need for rigorous standardization, multicenter comparison of results, and validation of ECFC isolation and culture procedures for clinical laboratory practice and robustness of results. To this end, we propose a standardized protocol for the isolation and expansion of ECFCs from umbilical cord and adult peripheral blood.
MeSH term(s)	Adult ; Humans ; Endothelial Cells ; Cell Culture Techniques ; Biology ; Australia ; Cells, Cultured ; Neovascularization, Physiologic
Language	English
Publishing date	2023-06-17
Publishing country	England
Document type	Multicenter Study ; Journal Article
ZDB-ID	2112661-6
ISSN	1538-7836 ; 1538-7933
ISSN (online)	1538-7836
ISSN	1538-7933
DOI	10.1016/j.jtha.2023.06.014
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Article ; Online: Monitoring of Lung Involvement in Rheumatologic Disease.

Paschalaki, Koralia E / Jacob, Joseph / Wells, Athol U

Respiration; international review of thoracic diseases

2016 Volume 91, Issue 2, Page(s) 89–98

Abstract: The monitoring of lung involvement in patients with connective tissue diseases is central to optimal long-term management and is directed towards: (a) the detection of supervening lung involvement not present at presentation and (b) the identification of ...

Abstract	The monitoring of lung involvement in patients with connective tissue diseases is central to optimal long-term management and is directed towards: (a) the detection of supervening lung involvement not present at presentation and (b) the identification of disease progression in established lung disease. For both goals, accurate surveillance requires multi-disciplinary evaluation with the integration of symptomatic change, serial pulmonary function trends and imaging data. Evaluated in isolation, each of these monitoring domains has significant limitations. Symptomatic change may be confounded by a wide variety of systemic factors. Pulmonary function tests provide the most reliable data, but are limited by measurement variability, the heterogeneity of functional patterns and the confounding effects of non-pulmonary factors. Chest radiography is insensitive to change but may provide rapid confirmation of major disease progression or alert the clinician to respiratory co-morbidities. Although high-resolution computed tomography has a central role in assessing disease severity, it should be used very selectively as a monitoring tool due to the associated radiation burden. Ancillary tests include echocardiography and exercise testing to proactively identify cases of pulmonary hypertension and worsening of oxygenation. In summary, a multi-disciplinary approach is essential for the identification of disease progression and prompt treatment of comorbidities that severely impact on the morbidity and mortality of disease.
MeSH term(s)	Humans ; Lung/diagnostic imaging ; Lung Diseases/diagnosis ; Lung Diseases/etiology ; Population Surveillance ; Respiratory Function Tests ; Rheumatic Diseases/complications
Language	English
Publishing date	2016
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	206674-9
ISSN	1423-0356 ; 0025-7931
ISSN (online)	1423-0356
ISSN	0025-7931
DOI	10.1159/000442890
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Article ; Online: Inhaled corticosteroids reduce senescence in endothelial progenitor cells from patients with COPD.

Paschalaki, Koralia / Rossios, Christos / Pericleous, Charis / MacLeod, Mairi / Rothery, Stephen / Donaldson, Gavin C / Wedzicha, Jadwiga A / Gorgoulis, Vassilis / Randi, Anna M / Barnes, Peter J

Thorax

2022 Volume 77, Issue 6, Page(s) 616–620

Abstract: Cellular senescence contributes to the pathophysiology of chronic obstructive pulmonary disease (COPD) and cardiovascular disease. Using endothelial colony-forming-cells (ECFC), we have demonstrated accelerated senescence in smokers and patients with ... ...

Abstract	Cellular senescence contributes to the pathophysiology of chronic obstructive pulmonary disease (COPD) and cardiovascular disease. Using endothelial colony-forming-cells (ECFC), we have demonstrated accelerated senescence in smokers and patients with COPD compared with non-smokers. Subgroup analysis suggests that ECFC from patients with COPD on inhaled corticosteroids (ICS) (n=14; eight on ICS) exhibited significantly reduced senescence (Senescence-associated-beta galactosidase activity, p21
MeSH term(s)	Administration, Inhalation ; Adrenal Cortex Hormones/pharmacology ; Adrenal Cortex Hormones/therapeutic use ; Cellular Senescence ; Endothelial Progenitor Cells ; Humans ; Pulmonary Disease, Chronic Obstructive
Chemical Substances	Adrenal Cortex Hormones
Language	English
Publishing date	2022-01-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	204353-1
ISSN	1468-3296 ; 0040-6376
ISSN (online)	1468-3296
ISSN	0040-6376
DOI	10.1136/thoraxjnl-2020-216807
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Article ; Online: Absence of JAK2V617F Mutated Endothelial Colony-Forming Cells in Patients With JAK2V617F Myeloproliferative Neoplasms and Splanchnic Vein Thrombosis.

Guy, Alexandre / Danaee, Anicee / Paschalaki, Koralia / Boureau, Lisa / Rivière, Etienne / Etienne, Gabriel / Mansier, Olivier / Laffan, Michael / Sekhar, Mallika / James, Chloe

HemaSphere

2020 Volume 4, Issue 3, Page(s) e364

Language	English
Publishing date	2020-05-05
Publishing country	United States
Document type	Letter
ISSN	2572-9241
ISSN (online)	2572-9241
DOI	10.1097/HS9.0000000000000364
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Article: Monitoring of Lung Involvement in Rheumatologic Disease

Paschalaki, Koralia E. / Jacob, Joseph / Wells, Athol U.

Respiration

2016 Volume 91, Issue 2, Page(s) 89–98

Institution	Airway Disease Section, National Heart and Lung Institute, Imperial College London, and Department of Radiology and Interstitial Lung Disease Unit, Royal Brompton Hospital, London, UK
Abstract	The monitoring of lung involvement in patients with connective tissue diseases is central to optimal long-term management and is directed towards: (a) the detection of supervening lung involvement not present at presentation and (b) the identification of disease progression in established lung disease. For both goals, accurate surveillance requires multi-disciplinary evaluation with the integration of symptomatic change, serial pulmonary function trends and imaging data. Evaluated in isolation, each of these monitoring domains has significant limitations. Symptomatic change may be confounded by a wide variety of systemic factors. Pulmonary function tests provide the most reliable data, but are limited by measurement variability, the heterogeneity of functional patterns and the confounding effects of non-pulmonary factors. Chest radiography is insensitive to change but may provide rapid confirmation of major disease progression or alert the clinician to respiratory co-morbidities. Although high-resolution computed tomography has a central role in assessing disease severity, it should be used very selectively as a monitoring tool due to the associated radiation burden. Ancillary tests include echocardiography and exercise testing to proactively identify cases of pulmonary hypertension and worsening of oxygenation. In summary, a multi-disciplinary approach is essential for the identification of disease progression and prompt treatment of comorbidities that severely impact on the morbidity and mortality of disease.
Keywords	Monitoring ; Connective tissue disease ; Symptomatic change ; Pulmonary function tests ; Chest radiography ; Pulmonary hypertension
Language	English
Publishing date	2016-01-07
Publisher	S. Karger AG
Publishing place	Basel, Switzerland
Document type	Article
Note	Thematic Review Series
ZDB-ID	206674-9
ISSN	1423-0356 ; 0025-7931
ISSN (online)	1423-0356
ISSN	0025-7931
DOI	10.1159/000442890
Database	Karger publisher's database

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Article ; Online: Pulmonary infection by SARS-CoV-2 induces senescence accompanied by an inflammatory phenotype in severe COVID-19: possible implications for viral mutagenesis.

Evangelou, Konstantinos / Veroutis, Dimitris / Paschalaki, Koralia / Foukas, Periklis G / Lagopati, Nefeli / Dimitriou, Marios / Papaspyropoulos, Angelos / Konda, Bindu / Hazapis, Orsalia / Polyzou, Aikaterini / Havaki, Sophia / Kotsinas, Athanassios / Kittas, Christos / Tzioufas, Athanasios G / de Leval, Laurence / Vassilakos, Demetris / Tsiodras, Sotirios / Stripp, Barry R / Papantonis, Argyris /

Blandino, Giovanni / Karakasiliotis, Ioannis / Barnes, Peter J / Gorgoulis, Vassilis G

The European respiratory journal

2022 Volume 60, Issue 2

Abstract: Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection of the respiratory system can progress to a multisystemic disease with aberrant inflammatory response. Cellular senescence promotes chronic inflammation, named senescence- ...

Abstract	Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection of the respiratory system can progress to a multisystemic disease with aberrant inflammatory response. Cellular senescence promotes chronic inflammation, named senescence-associated secretory phenotype (SASP). We investigated whether coronavirus disease 2019 (COVID-19) is associated with cellular senescence and SASP. Methods: Autopsy lung tissue samples from 11 COVID-19 patients and 43 age-matched non-COVID-19 controls with similar comorbidities were analysed by immunohistochemistry for SARS-CoV-2, markers of senescence and key SASP cytokines. Virally induced senescence was functionally recapitulated Results: SARS-CoV-2 was detected by immunocytochemistry and electron microscopy predominantly in AT2 cells. Infected AT2 cells expressed angiotensin-converting enzyme 2 and exhibited increased senescence (p16 Conclusions: We demonstrate that in severe COVID-19, AT2 cells infected by SARS-CoV-2 exhibit senescence and a proinflammatory phenotype.
MeSH term(s)	COVID-19 ; Cellular Senescence ; Cytokines/metabolism ; Humans ; Inflammation ; Interleukin-6 ; Lung/metabolism ; Mutagenesis ; Phenotype ; SARS-CoV-2
Chemical Substances	Cytokines ; Interleukin-6
Language	English
Publishing date	2022-08-18
Publishing country	England
Document type	Journal Article
ZDB-ID	639359-7
ISSN	1399-3003 ; 0903-1936
ISSN (online)	1399-3003
ISSN	0903-1936
DOI	10.1183/13993003.02951-2021
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Article ; Online: Hypoxia Increases the Potential for Neutrophil-mediated Endothelial Damage in Chronic Obstructive Pulmonary Disease.

Lodge, Katharine M / Vassallo, Arlette / Liu, Bin / Long, Merete / Tong, Zhen / Newby, Paul R / Agha-Jaffar, Danya / Paschalaki, Koralia / Green, Clara E / Belchamber, Kylie B R / Ridger, Victoria C / Stockley, Robert A / Sapey, Elizabeth / Summers, Charlotte / Cowburn, Andrew S / Chilvers, Edwin R / Li, Wei / Condliffe, Alison M

American journal of respiratory and critical care medicine

2022 Volume 205, Issue 8, Page(s) 903–916

Abstract: Rationale: ...

Abstract	Rationale:
MeSH term(s)	Endothelial Cells/metabolism ; Humans ; Hypoxia/metabolism ; Leukocyte Elastase/metabolism ; Neutrophils/metabolism ; Pulmonary Disease, Chronic Obstructive/metabolism ; Vascular System Injuries/metabolism
Chemical Substances	Leukocyte Elastase (EC 3.4.21.37)
Language	English
Publishing date	2022-02-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1180953-x
ISSN	1535-4970 ; 0003-0805 ; 1073-449X
ISSN (online)	1535-4970
ISSN	0003-0805 ; 1073-449X
DOI	10.1164/rccm.202006-2467OC
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Article: Endothelial progenitor cells in smokers are dysfunctional because of increased DNA damage and senescence

Paschalaki, Koralia E / Starke, Richard D / Mercado, Nicolas / Gorgoulis, Vassilis G / Barnes, Peter J / Randi, Anna M

lancet. 2013 Feb. 27, v. 381S1

2013

Abstract: BACKGROUND: Cardiovascular disease (CVD) is a major cause of death in smokers, especially in patients with chronic obstructive pulmonary disease (COPD). The molecular pathways that lead to endothelial dysfunction and CVD due to cigarette smoke remain ... ...

Abstract	BACKGROUND: Cardiovascular disease (CVD) is a major cause of death in smokers, especially in patients with chronic obstructive pulmonary disease (COPD). The molecular pathways that lead to endothelial dysfunction and CVD due to cigarette smoke remain unclear. DNA damage has been recognised as an important contributor in ageing disorders, including CVD. Circulating endothelial progenitor cells (EPC) are required for endothelial homoeostasis, and their dysfunction contributes to CVD. This study aimed to examine whether circulating EPC (also called blood outgrowth endothelial cells [BOEC]) from smokers and COPD patients are dysfunctional, and to investigate the role of DNA damage pathways in mediating endothelial dysfunction in these patients. METHODS: BOEC were isolated from peripheral blood samples received from 16 healthy non-smokers (five men, 11 women; mean age 57 years [SE 2·7]), ten healthy smokers (five men, five women; 57 years [2·6]), and 16 COPD patients (11 men, five women; 67 years [1·6]). Endothelial senescence was measured by senescence-associated β-galactosidase (SA-β-gal) activity. Protein levels of sirtuin 1 (SIRT1) were measured by western blotting, expression of p16, γ-H2AX, and 53BP1 by immunofluorescence, and p21 by western blotting and immunofluorescence. SIRT1 activity was measured with a SIRT1 fluorescent activity assay kit. FINDINGS: BOEC from smokers and COPD patients showed evidence of increased DNA double-strand breaks (increased γ-H2AX, 53BP1) compared with non-smokers. BOEC from healthy smokers and COPD patients displayed increased senescence (measured by SA-β-gal activity, p16, and p21) and decreased SIRT1 expression and activity compared with controls. SIRT1 protein levels and activity negatively correlated with senescence, indicating a regulatory role of SIRT1 on senescence. Interestingly, treatment of BOEC from COPD patients with a SIRT1 activator (resveratrol) rescued the senescent phenotype. INTERPRETATION: The results from our study demonstrate that BOEC from smokers and COPD patients display increased DNA damage and senescence, associated with reduced SIRT1 expression and activity. These defects may contribute to endothelial dysfunction and cardiovascular events in people who smoke and could potentially constitute therapeutic targets for intervention. FUNDING: Imperial College London.
Keywords	DNA damage ; Western blotting ; beta-galactosidase ; blood ; cardiovascular diseases ; cigarettes ; correlation ; death ; endothelial cells ; fluorescence ; fluorescent antibody technique ; men ; patients ; phenotype ; resveratrol ; senescence ; smoke ; stem cells ; women
Language	English
Dates of publication	2013-0227
Size	p. S84.
Publishing place	Elsevier Ltd.
Document type	Article
ZDB-ID	3306-6
ISSN	1474-547X ; 0023-7507 ; 0140-6736
ISSN (online)	1474-547X
ISSN	0023-7507 ; 0140-6736
DOI	10.1016/S0140-6736(13)60524-3
Database	NAL-Catalogue (AGRICOLA)

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