Article ; Online: Breast cancer amelioration by Butea monosperma in-vitro and in-vivo.
2018 Volume 217, Page(s) 54–62
Abstract: Ethnopharmacological relevance: Butea monosperma belonging to family Fabaceae is used in the Indian traditional medicine (Ayurveda) for various ailments including abdominal tumors and possess anti-estrogenic activity.: Aim of the study: The present ... ...
| Abstract | Ethnopharmacological relevance: Butea monosperma belonging to family Fabaceae is used in the Indian traditional medicine (Ayurveda) for various ailments including abdominal tumors and possess anti-estrogenic activity. Aim of the study: The present study is aimed at investigating the chemo-preventive potential of Butea monosperma in breast cancer and elucidating it's mechanism of action by assessing its effect on key processes like apoptosis, angiogenesis and metastasis. Methods: Cytotoxic potential of methanol extract of Butea monosperma flower (MEBM) was tested in MCF-7 (estrogen receptor positive), MDA-MB-231 (triple negative) and MDA-MB-453 (HER2 positive) human breast cancer cells by MTT assay. Chemo-preventive potential was evaluated in-vivo in Methylnitrosourea (MNU) induced mammary cancer in nulliparous Sprague-Dawley rats. The mechanism for anticancer potential was screened by in-vitro studies involving Annexin V- FITC assay (apoptosis), Chick Chorioallantoic Membrane assay (angiogenesis) and Migration assay (metastasis). Statistical analysis was done by one way and two way ANOVA (for Growth Rate and feed consumption efficiency) followed by post hoc Bonferroni's test with P value < 0.05. Results: It is observed that the exposure of MEBM, at various concentrations and time intervals to different cell lines, resulted in decreased cell proliferation. The IC Conclusion: Selective cytotoxic activity in MCF-7 estrogen positive breast cancer cells and inhibition of growth of mammary carcinoma in-vivo by methanol extract of Butea monosperma flowers (MEBM) suggests chemo-prevention through modulation of estrogen and progesterone receptor, apoptotic, anti-angiogenesis and anti-metastatic activity. |
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| MeSH term(s) | Animals ; Antineoplastic Agents, Phytogenic/isolation & purification ; Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis/drug effects ; Breast Neoplasms/chemically induced ; Breast Neoplasms/drug therapy ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Butea/chemistry ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Chick Embryo ; Dose-Response Relationship, Drug ; Female ; Humans ; MCF-7 Cells ; Methylnitrosourea ; Neoplasm Invasiveness ; Neovascularization, Pathologic ; Phytotherapy ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology ; Plants, Medicinal ; Rats, Sprague-Dawley ; Receptors, Estrogen/drug effects ; Receptors, Estrogen/metabolism ; Receptors, Progesterone/drug effects ; Receptors, Progesterone/metabolism ; Signal Transduction/drug effects ; Time Factors |
| Chemical Substances | Antineoplastic Agents, Phytogenic ; Plant Extracts ; Receptors, Estrogen ; Receptors, Progesterone ; Methylnitrosourea (684-93-5) |
| Language | English |
| Publishing date | 2018-01-31 |
| Publishing country | Ireland |
| Document type | Journal Article |
| ZDB-ID | 134511-4 |
| ISSN | 1872-7573 ; 0378-8741 |
| ISSN (online) | 1872-7573 |
| ISSN | 0378-8741 |
| DOI | 10.1016/j.jep.2017.12.026 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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