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Article ; Online: The soil and the seed: The relationship between Descemet's membrane and the corneal endothelium.

Petrela, Redion B / Patel, Sangita P

2022 Volume 227, Page(s) 109376

Abstract: Descemet's membrane (DM), the basement membrane of the corneal endothelium, is formed from the extracellular matrix (ECM) secreted by corneal endothelial cells. The ECM supports the growth and function of the corneal endothelial cells. Changes to DM are ... ...

Abstract	Descemet's membrane (DM), the basement membrane of the corneal endothelium, is formed from the extracellular matrix (ECM) secreted by corneal endothelial cells. The ECM supports the growth and function of the corneal endothelial cells. Changes to DM are central to the diagnosis of the most common corneal endothelial disease, Fuchs endothelial corneal dystrophy (FECD). Changes in DM are also noted in systemic diseases such as diabetes mellitus. In FECD, the DM progressively accumulates guttae, "drop-like deposits" that disrupt the corneal endothelial cell monolayer. While the pathophysiologic changes to corneal endothelial cells in the course of FECD have been well described and reviewed, the changes to DM have received limited attention. The reciprocity of influence between the corneal endothelial cells and DM demands full attention to the latter in our search for novel treatment and preventive strategies. In this review, we discuss what is known about the formation and composition of DM and how it changes in FECD and other conditions. We review characteristics of guttae and the interplay between corneal endothelial cells and guttae, particularly as it might apply to future cell-based and genetic therapies for FECD.
MeSH term(s)	Humans ; Endothelium, Corneal ; Descemet Membrane ; Soil ; Endothelial Cells ; Fuchs' Endothelial Dystrophy
Chemical Substances	Soil
Language	English
Publishing date	2022-12-30
Publishing country	England
Document type	Journal Article ; Review ; Research Support, N.I.H., Extramural
ZDB-ID	80122-7
ISSN	1096-0007 ; 0014-4835
ISSN (online)	1096-0007
ISSN	0014-4835
DOI	10.1016/j.exer.2022.109376
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Article ; Online: The efficacy of 2 different phakic intraocular lens implant in keratoconus as an isolated procedure or combined with collagen crosslinking and intra-stromal corneal ring segments: a systematic review and meta-analysis.

Fard, Ali Mahdavi / Patel, Sangita P / Nader, Nader D

International ophthalmology

2023 Volume 43, Issue 11, Page(s) 4383–4393

Abstract: Purpose: To compare the efficacy of phakic intra-ocular lenses in isolation or in combination with corneal crosslinking (CXL) and/or intra-stromal corneal ring segments (ICRS) in keratoconus.: Methods: Data extracted from the publications meeting the ...

Abstract	Purpose: To compare the efficacy of phakic intra-ocular lenses in isolation or in combination with corneal crosslinking (CXL) and/or intra-stromal corneal ring segments (ICRS) in keratoconus. Methods: Data extracted from the publications meeting the selection. The outcome parameters included mean pre- and post-operative uncorrected distance visual acuity, corrected distance visual acuity (CDVA), sphere and cylinder of refraction and complications. Available data analyzed with Cochrane Review Manager. Results: A total of 23 studies including 464 eyes were included. All the parameters showed significant improvement in all subgroups other than CDVA in ACPIOL + CXL subgroup and cylinder in PIOL + CXL subgroups. There was not a significant difference between PCPIOL and ACPIOL in the outcomes, exception was more improvement of CDVA in "ACPIOL only" than" PCPIOL only" subgroup. Conclusion: Both PCPIOLs and ACPIOLs are comparably safe and efficient options in management of KCN and their efficacy significantly improves when combined with CXL/ICRS.
MeSH term(s)	Humans ; Phakic Intraocular Lenses ; Photosensitizing Agents/therapeutic use ; Keratoconus/drug therapy ; Keratoconus/surgery ; Riboflavin/therapeutic use ; Collagen/pharmacology ; Collagen/therapeutic use ; Corneal Topography ; Cross-Linking Reagents/therapeutic use ; Corneal Stroma/surgery
Chemical Substances	Photosensitizing Agents ; Riboflavin (TLM2976OFR) ; Collagen (9007-34-5) ; Cross-Linking Reagents
Language	English
Publishing date	2023-07-20
Publishing country	Netherlands
Document type	Meta-Analysis ; Systematic Review ; Journal Article ; Review
ZDB-ID	800087-6
ISSN	1573-2630 ; 0165-5701
ISSN (online)	1573-2630
ISSN	0165-5701
DOI	10.1007/s10792-023-02813-z
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Article ; Online: Risk assessment of variant Creutzfeldt-Jakob disease in corneal transplantation.

Desilets, Jeffrey / Mittal, Abhiniti / Sellick, John A / Patel, Sangita P

American journal of ophthalmology case reports

2023 Volume 30, Page(s) 101856

Abstract: Purpose: While corneal transplantation is known to have a potential risk of transmission of variant Creutzfeldt-Jacob Disease (vCJD), the magnitude of this risk has not been quantified.: Observations: A case report is presented of a 73 year-old man ... ...

Abstract	Purpose: While corneal transplantation is known to have a potential risk of transmission of variant Creutzfeldt-Jacob Disease (vCJD), the magnitude of this risk has not been quantified. Observations: A case report is presented of a 73 year-old man with a penetrating keratoplasty graft from corneal tissue that was recalled after transplantation due to risk of vCJD because it was later discovered that the donor had traveled to the United Kingdom (UK). Probabilities of vCJD transmission were extrapolated using Creutzfeldt-Jacob Disease (CJD) mortality (incidence) rate, all-cause death rate, and rate of recovery for intended transplantation. Conclusions: An overestimate of the risk of transplanting a cornea infected with vCJD in 2018 was 1 in 940,000. The true risk of vCJD transmission would be even lower due to an incomplete infectivity rate. We conclude that the risk of transmission of latent vCJD by corneal transplantation from a donor who traveled to the UK from 1980 to 1996 is exceedingly low.
Language	English
Publishing date	2023-05-02
Publishing country	United States
Document type	Case Reports
ISSN	2451-9936
ISSN (online)	2451-9936
DOI	10.1016/j.ajoc.2023.101856
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Article: A Case Report Illustrating the Postoperative Course of Descemetorhexis without Endothelial Keratoplasty with Topical Netarsudil Therapy.

Ploysangam, Pimpiroon / Patel, Sangita P

Case reports in ophthalmological medicine

2019 Volume 2019, Page(s) 6139026

Abstract: Fuchs endothelial corneal dystrophy (FECD) is the most common indication for corneal transplantation in the United States. Recently, descemetorhexis without endothelial keratoplasty (DWEK) or Descemet's stripping only (DSO) has become an attractive ... ...

Abstract	Fuchs endothelial corneal dystrophy (FECD) is the most common indication for corneal transplantation in the United States. Recently, descemetorhexis without endothelial keratoplasty (DWEK) or Descemet's stripping only (DSO) has become an attractive alternative to corneal transplantation for these patients. DSO circumvents the challenges associated with cadaveric donor corneal transplantation by tapping into the potential of the patient's own corneal endothelium to repair defects. Outcomes have been variable with emerging knowledge on predictive factors for success. Our case describes a 51-year-old patient with visually significant confluent central guttae from FECD who underwent a successful DSO with immediate post-operative use of the Rho-associated protein kinase (ROCK) inhibitor (netarsudil). We report the preoperative and post-operative slit lamp images, specular microscopy data, and corneal topography, thickness, and densitometry data. These represent a unique data set for this new surgical treatment option for FECD. Despite a small descemetorhexis, we show the improvement in corneal thickness and opacity extends beyond the boundaries of the descemetorhexis. Early initiation of a ROCK inhibitor was a successful treatment for this patient.
Language	English
Publishing date	2019-10-13
Publishing country	United States
Document type	Case Reports
ZDB-ID	2659091-8
ISSN	2090-6730 ; 2090-6722
ISSN (online)	2090-6730
ISSN	2090-6722
DOI	10.1155/2019/6139026
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Article: The bull's eye: are we off-target for corneal endothelial cell physiology?

Patel, Sangita P

Journal of ophthalmic & vision research

2013 Volume 8, Issue 1, Page(s) 83–85

Language	English
Publishing date	2013-06-25
Publishing country	India
Document type	Journal Article
ISSN	2008-2010
ISSN	2008-2010
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Article ; Online: A portable, low-cost practice model for microsurgical skills training.

Greyner-Almeida, Henry D / Mahdavi Fard, Ali / Chen, Chi / Zhao, Jiwei / Patel, Sangita P

International ophthalmology

2022 Volume 42, Issue 8, Page(s) 2323–2333

Abstract: Purpose: We describe a portable practice model for acquisition of microsurgical skills using widely available inexpensive tools and materials as a model in learning ophthalmic corneal suturing skills.: Methods: Interested participants without prior ... ...

Abstract	Purpose: We describe a portable practice model for acquisition of microsurgical skills using widely available inexpensive tools and materials as a model in learning ophthalmic corneal suturing skills. Methods: Interested participants without prior microsurgery experience affiliated with the Jacobs School of Medicine and Biomedical Sciences with no prior microsurgical experience qualified to participate. Each participant completed written informed consent. We developed a 3-dimensional micro-stellated icosahedron model using microtubules, monofilament fishing line, jewelers' forceps, and a basic laboratory dissection microscope. We tested this model in improving microsurgical skills in a randomized, controlled intervention trial. Following a pre-assessment task of passing a microsurgical needle and performing a tie, participants were randomized to a control or an intervention (building the micro-stellated icosahedrons) group. The assessment task was repeated after two weeks. Videos of pre- and post-assessments were rated by two masked ophthalmologists. Technique scores and time to complete microsurgical tasks were analyzed to determine improvement in skills. Results: A total of 27 microsurgically naïve participants were recruited and randomized (14 Intervention / 13 Control). Comparing pre- and post-assessments, the intervention group showed significant decrease in time required to pass the needle (P = 0.018) and significant improvement in technical scores. (P = 0.001). In the control group, there was no significant decrease in time or improvement in technical scores. Conclusions: The portable inexpensive micro-stellated icosahedron skills acquisition model is an effective practice model to acquire skills necessary to perform a microsurgical tie. The similarity in dimensions between the model and the eye suggests translatability to ophthalmic surgery.
MeSH term(s)	Clinical Competence ; Humans ; Microsurgery/education ; Models, Educational ; Ophthalmology/education
Language	English
Publishing date	2022-01-29
Publishing country	Netherlands
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	800087-6
ISSN	1573-2630 ; 0165-5701
ISSN (online)	1573-2630
ISSN	0165-5701
DOI	10.1007/s10792-022-02229-1
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Zs.A 1479: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: Influence of eyelid pigmentation on the diagnosis of meibomian gland dysfunction.

Blumberg, Max J / Millen, Amy E / Patel, Sangita P

Clinical ophthalmology (Auckland, N.Z.)

2019 Volume 13, Page(s) 1815–1821

Abstract: Purpose: To determine whether reliance on eyelid margin vascularization as a diagnostic criterion for meibomian gland dysfunction (MGD) results in underdiagnosis of MGD in individuals with dark skin pigmentation.: Patients and methods: This cross- ... ...

Abstract	Purpose: To determine whether reliance on eyelid margin vascularization as a diagnostic criterion for meibomian gland dysfunction (MGD) results in underdiagnosis of MGD in individuals with dark skin pigmentation. Patients and methods: This cross-sectional study enrolled consecutive cornea clinic patients in Buffalo, New York. Eyelid margin vascularization was graded qualitatively from slit-lamp photos. Skin pigmentation was quantified from digital photos using red/green/blue (RGB) pixel analysis and dichotomized using the RGB median. MGD was defined as abnormal quantity or quality of meibum or increased pressure required to express meibum. Additional testing included infrared meibography, Schirmer's testing, and a dry eye questionnaire. Sensitivity of MGD diagnosis by visualization of vascularization, compared to diagnosis by expression of meibum, was estimated with and without stratification by skin pigmentation. Results: Among 47 participants, 15-79 years old, meibomian gland truncation/dropout, abnormal tear production, and dry eye symptoms affected individuals of all skin pigmentations. Eyelid margin vascularization was less common in subjects with dark (n=21%) compared to light pigmentation (65%; Conclusion: Our findings highlight the importance of gland expression and suggest limiting reliance on eyelid margin vascularization for MGD diagnosis, especially in those with dark eyelid skin pigmentation.
Language	English
Publishing date	2019-09-19
Publishing country	New Zealand
Document type	Journal Article
ISSN	1177-5467
ISSN	1177-5467
DOI	10.2147/OPTH.S222451
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Article ; Online: Bilateral ocular injuries with globe perforation and retinitis sclopetaria from birdshot shotgun: A case report and review of the literature.

Ku, Cristy A / Jeng, Franklin / Reynolds, Andrew L / Patel, Sangita P

Trauma case reports

2020 Volume 28, Page(s) 100329

Language	English
Publishing date	2020-06-28
Publishing country	Netherlands
Document type	Case Reports
ZDB-ID	2835433-3
ISSN	2352-6440 ; 2352-6440
ISSN (online)	2352-6440
ISSN	2352-6440
DOI	10.1016/j.tcr.2020.100329
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Article ; Online: Effect of Physiological Oxygen on Primary Human Corneal Endothelial Cell Cultures.

Patel, Sangita P / Calle Gonzalez, Brayan / Paone, Nataliia / Mueller, Christian / Floss, Jamie C / Sousa, Maria E / Shi, Michael Y

Translational vision science & technology

2022 Volume 11, Issue 2, Page(s) 33

Abstract: Purpose: Primary human corneal endothelial cells (HCEnCs) cultured in room air are exposed to significantly higher O2 concentrations [O2] than what is normally present in the eye. We evaluated the growth and metabolism of HCEnCs cultured under ... ...

Abstract	Purpose: Primary human corneal endothelial cells (HCEnCs) cultured in room air are exposed to significantly higher O2 concentrations [O2] than what is normally present in the eye. We evaluated the growth and metabolism of HCEnCs cultured under physiological [O2] (2.5%; [O2]2.5) and room air ([O2]A). Methods: Primary cultures of HCEnCs from normal donors and donors with Fuchs dystrophy were grown at [O2]2.5 and [O2]A. Growth and morphology were compared using phase-contrast microscopy, zonula occludens (ZO-1) localization, cell density measurements, and senescence marker staining. CD44 (cell quality) and HIF-1α (hypoxia-inducible factor-1α) levels were evaluated by Western blotting. Cell adaptability to a reversal of [O2] growth conditions was measured with cell viability assays, and cell metabolism was assessed via oxygen consumption and extracellular acidification rates. Results: HCEnCs grown at [O2]A and [O2]2.5 displayed similar morphologies, ZO-1 localization, CD44 expression, and senescence. Cells from donors with Fuchs dystrophy grew better at [O2]2.5 than at [O2]A. HIF-1α was undetectable. Cells displayed greater viability at [O2]2.5 than at [O2]A. HCEnCs showed significantly greater proton leak (P < 0.01), nonmitochondrial oxygen consumption (P < 0.01), and spare capacity (P < 0.05) for oxygen consumption rates, and greater basal glycolysis (P < 0.05) with a decreased glycolytic reserve capacity (P < 0.05) for extracellular acidification rates. Conclusions: Primary HCEnCs show unique metabolic characteristics at physiologic [O2]. The effect of [O2] for optimization of HCEnC culture conditions should be considered. Translational relevance: With the advance of cell-based therapeutics for corneal endothelial diseases, [O2] should be considered an important variable in the optimization of HCEnC culture conditions.
MeSH term(s)	Cell Count ; Endothelial Cells ; Fuchs' Endothelial Dystrophy ; Humans ; Oxygen/pharmacology
Chemical Substances	Oxygen (S88TT14065)
Language	English
Publishing date	2022-03-17
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2674602-5
ISSN	2164-2591 ; 2164-2591
ISSN (online)	2164-2591
ISSN	2164-2591
DOI	10.1167/tvst.11.2.33
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Article ; Online: SLC4A11 and the Pathophysiology of Congenital Hereditary Endothelial Dystrophy.

Patel, Sangita P / Parker, Mark D

BioMed research international

2015 Volume 2015, Page(s) 475392

Abstract: Congenital hereditary endothelial dystrophy (CHED) is a rare autosomal recessive disorder of the corneal endothelium characterized by nonprogressive bilateral corneal edema and opacification present at birth. Here we review the current knowledge on the ... ...

Abstract	Congenital hereditary endothelial dystrophy (CHED) is a rare autosomal recessive disorder of the corneal endothelium characterized by nonprogressive bilateral corneal edema and opacification present at birth. Here we review the current knowledge on the role of the SLC4A11 gene, protein, and its mutations in the pathophysiology and clinical presentation of CHED. Individuals with CHED have mutations in SLC4A11 which encodes a transmembrane protein in the SLC4 family of bicarbonate transporters. The expression of SLC4A11 in the corneal endothelium and inner ear patterns the deficits seen in CHED with corneal edema and hearing loss (Harboyan syndrome). slc4a11-null-mouse models recapitulate the CHED disease phenotype, thus establishing a functional role for SLC4A11 in CHED. However, the transport function of SLC4A11 remains unsettled. Some of the roles that have been attributed to SLC4A11 include H(+) and NH4 (+) permeation, electrogenic Na(+)-H(+) exchange, and water transport. Future studies of the consequences of SLC4A11 dysfunction as well as further understanding of corneal endothelial ion transport will help clarify the involvement of SLC4A11 in the pathophysiology of CHED.
MeSH term(s)	Animals ; Anion Transport Proteins/genetics ; Anion Transport Proteins/metabolism ; Antiporters/genetics ; Antiporters/metabolism ; Corneal Dystrophies, Hereditary/physiopathology ; Epithelium, Corneal/metabolism ; Genetic Markers/genetics ; Genetic Predisposition to Disease/genetics ; Humans ; Incidence ; Ion Channel Gating ; Mice ; Models, Biological ; Risk Factors
Chemical Substances	Anion Transport Proteins ; Antiporters ; Genetic Markers ; SLC4A11 protein, human
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2015/475392
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