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Article ; Online: Process optimization and kinetic study of biodegradation of dimethyl phthalate by KS2, a novel strain of Micrococcus sp.

Patil, Sangram Shamrao / Jena, Hara Mohan

2023 Volume 13, Issue 1, Page(s) 3900

Abstract: The present study elucidates identification and characterization of dimethyl phthalate (DMP) degrading novel bacterial strain, Micrococcus sp. KS2, isolated from soil contaminated with municipal wastewater. Statistical designs were exercised to achieve ... ...

Abstract	The present study elucidates identification and characterization of dimethyl phthalate (DMP) degrading novel bacterial strain, Micrococcus sp. KS2, isolated from soil contaminated with municipal wastewater. Statistical designs were exercised to achieve optimum values of process parameters for DMP degradation by Micrococcus sp. KS2. The screening of the ten important parameters was performed by applying Plackett-Burman design, and it delivered three significant factors (pH, temperature, and DMP concentration). Further, response surface methodology involving central composite design (CCD) was implemented to examine mutual interactions among variables and achieve their optimal response. The predicted response indicated that maximum DMP degradation (99.67%) could be attained at pH 7.05, temperature 31.5 °C and DMP 289.19 mg/l. The strain KS2 was capable of degrading up to 1250 mg/l of DMP in batch mode and it was observed that oxygen was limiting factor in the DMP degradation. Kinetic modeling of DMP biodegradation indicated that Haldane model fitted well with the experimental data. During DMP degradation, monomethyl phthalate (MMP) and phthalic acid (PA) were identified as degradation metabolites. This study provides insight into DMP biodegradation process and proposes that Micrococcus sp. KS2 is a potential bacterial candidate to treat effluent containing DMP.
MeSH term(s)	Micrococcus/metabolism ; Phthalic Acids/metabolism ; Biodegradation, Environmental ; Kinetics ; Sewage/microbiology
Chemical Substances	dimethyl phthalate (08X7F5UDJM) ; Phthalic Acids ; Sewage
Language	English
Publishing date	2023-03-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-29256-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Biodegradation of diethyl phthalate from synthetic wastewater in a batch operated internal loop airlift bioreactor

Patil, Sangram Shamrao / Jena, Hara Mohan

International biodeterioration & biodegradation. 2019 Sept., v. 143

2019

Abstract: In the present study, biodegradation of diethyl phthalate (DEP) was carried out with a mixed culture of aerobic bacterial strains isolated from soil contaminated with municipal wastewater. They were identified as Bacillus sp. and Micrococcus sp. based on ...

Abstract	In the present study, biodegradation of diethyl phthalate (DEP) was carried out with a mixed culture of aerobic bacterial strains isolated from soil contaminated with municipal wastewater. They were identified as Bacillus sp. and Micrococcus sp. based on morphological, biochemical and molecular characteristics. For the treatment of synthetic wastewater containing DEP, an internal loop airlift bioreactor was designed and fabricated. Experiments were carried out for various initial concentrations (100-1500 mg/l) in a batch mode using a mixed culture of isolates. Mass balance and stoichiometric investigation suggested efficient degradation of DEP with a yield of 0.81 g/g if two-thirds of carbon from substrate converted to biomass. Gas chromatography-Mass spectroscopy analysis detected monoethyl phthalate (MEP) and phthalic acid (PA) sequentially to confirm hydrolysis of DEP. Enzymatic assay of the esterase production (929.5 U/l for 1000 mg/l of DEP) was responsible for hydrolysis of DEP to MEP. Growth kinetic study delivered the best fit of experimental data with the Haldane model with the correlation coefficient (r2) 0.95. Biokinetic constants indicated that the high concentrations of DEP inhibited the growth rate of bacteria while dissolved oxygen estimation suggested that the DEP biodegradation was oxygen limited.
Keywords	Bacillus (bacteria) ; bacteria ; biodegradation ; biomass ; bioreactors ; carbon ; diethyl phthalate ; dissolved oxygen ; esterases ; gas chromatography-mass spectrometry ; hydrolysis ; Micrococcus ; mixed culture ; models ; municipal wastewater ; oxygen ; phthalic acid ; polluted soils ; stoichiometry
Language	English
Dates of publication	2019-09
Publishing place	Elsevier Ltd
Document type	Article
ISSN	0964-8305
DOI	10.1016/j.ibiod.2019.104728
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effectiveness of mindfulness meditation (Vipassana) in the management of chronic low back pain.

Patil, Sangram G

Indian journal of anaesthesia

2010 Volume 53, Issue 2, Page(s) 158–163

Abstract: Summary: Chronic low back pain (CLBP) is challenging to treat with its significant psychological and cognitive behavioural element involved. Mindfulness meditation helps alter the behavioural response in chronic pain situations. Significant body of ... ...

Abstract	Summary: Chronic low back pain (CLBP) is challenging to treat with its significant psychological and cognitive behavioural element involved. Mindfulness meditation helps alter the behavioural response in chronic pain situations. Significant body of research in the filed of mindfulness meditation comes from the work of Dr Kabat-Zinn. The current evidence in the field, though not grade one, shows that there is a place for mindfulness meditation in managing chronic pain conditions including CLBP. Further research to test the usefulness of mindfulness in CLBP should involve good quality randomized controlled trials of pure mindfulness based technique in matched subjects.
Language	English
Publishing date	2010-02-09
Publishing country	India
Document type	Journal Article
ZDB-ID	412570-8
ISSN	0976-2817 ; 0019-5049
ISSN (online)	0976-2817
ISSN	0019-5049
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Article ; Online: Microbial biosurfactants for oil spill remediation: pitfalls and potentials.

Patel, Seema / Homaei, Ahmad / Patil, Sangram / Daverey, Achlesh

Applied microbiology and biotechnology

2018 Volume 103, Issue 1, Page(s) 27–37

Abstract: Spillage of fossil-based oils during their conveyance through water conduits are sporadic, but significant environmental disasters. As the viscous hydrocarbons of the crude oils spread on water surface and choke aquatic life to death, their effective ... ...

Abstract	Spillage of fossil-based oils during their conveyance through water conduits are sporadic, but significant environmental disasters. As the viscous hydrocarbons of the crude oils spread on water surface and choke aquatic life to death, their effective degradation is crucial for ecological balance. Though chemical and mechanical means are conventional ways to tackle the issues, they are riddled with limitations. In this scenario, coercing the biosurfactant-producing bacteria and fungi are promising avenues. Biosurfactants, the amphiphilic compounds, are capable of reducing interfacial tension, dispersing the oil particles, and degrading them into non-toxic debris. Among the vast array of biosurfactants, the trio of rhamnolipid, sophorolipid, and surfactin have been characterized well. Among the microbes, only Pseudomonas, Bacillus, and Candida have been evaluated, while there can be other exploitable candidates. In this regard, this review discusses the scopes and hurdles in utilization of the microbial surface-active compounds for oil spill management.
MeSH term(s)	Bacteria/chemistry ; Bacteria/metabolism ; Environmental Restoration and Remediation/methods ; Fungi/chemistry ; Fungi/metabolism ; Glycolipids/chemistry ; Lipopeptides/chemistry ; Oleic Acids/chemistry ; Petroleum Pollution ; Surface-Active Agents/chemistry
Chemical Substances	Glycolipids ; Lipopeptides ; Oleic Acids ; Surface-Active Agents ; rhamnolipid ; sophorolipid
Language	English
Publishing date	2018-10-21
Publishing country	Germany
Document type	Journal Article ; Review
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-018-9434-2
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Article: Assessment of bioavailability of gold bhasma in human participants - A pilot study.

Patil-Bhole, Trupti / Patil, Sangram / Wele, Asmita A

Journal of Ayurveda and integrative medicine

2018 Volume 9, Issue 4, Page(s) 294–297

Abstract: Bioavailability of the well-known Ayurvedic drug Swarnabhasma (gold bhasma or calcined gold) is unknown. It is orally administered either sublingually or directly with various Anupanas like black pepper powder (Piper nigrum Linn.) and cow ghee in the ... ...

Abstract	Bioavailability of the well-known Ayurvedic drug Swarnabhasma (gold bhasma or calcined gold) is unknown. It is orally administered either sublingually or directly with various Anupanas like black pepper powder (Piper nigrum Linn.) and cow ghee in the dose range of 15-240 mg by Ayurvedic physicians. Study of bioavailability of Swarnabhasma is necessary as this metal-derived drug is administered for long duration for rejuvenation. The pilot study was carried out in healthy human male participants to assess bioavailability of Swarnabhasma in three doses, viz. 30 mg plain sublingual, 30 mg oral dose mixed with black pepper powder (250 mg) and cow ghee (2.5 gm); and 240 mg oral dose mixed with black pepper powder (250 mg) and cow ghee (2.5 gm). Blood samples were withdrawn at 0, 1, 2 and 4 h after administration of dose. Estimation of gold levels in blood was carried out by inductively coupled plasma mass spectrometry (ICP-MS). Results show that gold is absorbed in traces from single dose of Swarnabhasma. Maximum concentration of gold was bioavailable from 30 mg sublingual dose with C
Language	English
Publishing date	2018-11-17
Publishing country	United States
Document type	Journal Article
ISSN	0975-9476
ISSN	0975-9476
DOI	10.1016/j.jaim.2018.04.002
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Article: Appendicular Neuroendocrine Neoplasm is Associated with Acute Appendicitis - Don't Miss the Boat.

Khan, Khalid / Patil, Sangram / Roomi, Shahid / Shiwani, Muhammad Hanif

Chirurgia (Bucharest, Romania : 1990)

2019 Volume 114, Issue 4, Page(s) 461–466

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Acute Disease ; Adult ; Appendectomy ; Appendiceal Neoplasms/complications ; Appendiceal Neoplasms/diagnosis ; Appendiceal Neoplasms/surgery ; Appendicitis/complications ; Appendicitis/diagnosis ; Appendicitis/surgery ; Appendix/surgery ; Diagnostic Errors ; Female ; Humans ; Laparoscopy ; Male ; Neoplasm Recurrence, Local ; Neuroendocrine Tumors/complications ; Neuroendocrine Tumors/diagnosis ; Neuroendocrine Tumors/surgery ; Retrospective Studies
Language	English
Publishing date	2019-09-12
Publishing country	Romania
Document type	Journal Article
ZDB-ID	419244-8
ISSN	1842-368X ; 1221-9118 ; 0009-4730 ; 0377-5003
ISSN (online)	1842-368X
ISSN	1221-9118 ; 0009-4730 ; 0377-5003
DOI	10.21614/chirurgia.114.4.461
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Zs.MO 620: Show issues

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Article ; Online: Migrated Biliary Stent Causing Entero-enteric Fistula.

Patil, Sangram / Roomi, Shahid / Shiwani, M H

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract

2016 Volume 21, Issue 1, Page(s) 205–206

Language	English
Publishing date	2016-08-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	2012365-6
ISSN	1873-4626 ; 1934-3213 ; 1091-255X
ISSN (online)	1873-4626 ; 1934-3213
ISSN	1091-255X
DOI	10.1007/s11605-016-3213-0
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Article: Assessment of bioavailability of gold bhasma in human participants – A pilot study

Patil-Bhole, Trupti / Patil, Sangram / Wele, Asmita A.

Transdisciplinary University, Bangalore and World Ayurveda Foundation Journal of Ayurveda and integrative medicine. 2018 Oct., Dec., v. 9, no. 4

2018

Abstract	Bioavailability of the well-known Ayurvedic drug Swarnabhasma (gold bhasma or calcined gold) is unknown. It is orally administered either sublingually or directly with various Anupanas like black pepper powder (Piper nigrum Linn.) and cow ghee in the dose range of 15–240 mg by Ayurvedic physicians. Study of bioavailability of Swarnabhasma is necessary as this metal-derived drug is administered for long duration for rejuvenation. The pilot study was carried out in healthy human male participants to assess bioavailability of Swarnabhasma in three doses, viz. 30 mg plain sublingual, 30 mg oral dose mixed with black pepper powder (250 mg) and cow ghee (2.5 gm); and 240 mg oral dose mixed with black pepper powder (250 mg) and cow ghee (2.5 gm). Blood samples were withdrawn at 0, 1, 2 and 4 h after administration of dose. Estimation of gold levels in blood was carried out by inductively coupled plasma mass spectrometry (ICP-MS). Results show that gold is absorbed in traces from single dose of Swarnabhasma. Maximum concentration of gold was bioavailable from 30 mg sublingual dose with Cₘₐₓ 0.983 μg/L at 2 h (Tₘₐₓ). Oral dose of 30 mg Swarnabhasma mixed with black pepper powder and ghee showed faster absorption with Tₘₐₓ at 1 h and Cₘₐₓ 0.867 μg/L, and 240 mg dose with black pepper and ghee showed Cₘₐₓ 0.668 μg/L and Tₘₐₓ at 2 h.
Keywords	Ayurvedic medicine ; Piper nigrum ; absorption ; atomic absorption spectrometry ; bioavailability ; black pepper ; cows ; drugs ; ghee ; gold ; humans ; males ; sublingual administration
Language	English
Dates of publication	2018-10
Size	p. 294-297.
Publishing place	Elsevier B.V.
Document type	Article
ISSN	0975-9476
DOI	10.1016/j.jaim.2018.04.002
Database	NAL-Catalogue (AGRICOLA)

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Article: Kinematic analysis of motor performance in robot-assisted surgery: a preliminary study.

Nisky, Ilana / Patil, Sangram / Hsieh, Michael H / Okamura, Allison M

Studies in health technology and informatics

2013 Volume 184, Page(s) 302–308

Abstract: The inherent dynamics of the master manipulator of a teleoperated robot-assisted surgery (RAS) system can affect the movements of a human operator, in comparison with free-space movements. To measure the effects of these dynamics on operators with ... ...

Abstract	The inherent dynamics of the master manipulator of a teleoperated robot-assisted surgery (RAS) system can affect the movements of a human operator, in comparison with free-space movements. To measure the effects of these dynamics on operators with differing levels of surgical expertise, a da Vinci Si system was instrumented with a custom surgeon grip fixture and magnetic pose trackers. We compared users' performance of canonical motor control movements during teleoperation with the manipulator and freehand cursor control, and found significant differences in several aspects of motion, including target acquisition error, movement speed, and acceleration. In addition, there was preliminary evidence for differences between experts and novices. These findings could impact robot design, control, and training methods for RAS.
MeSH term(s)	Arm/physiology ; Computer Simulation ; Humans ; Man-Machine Systems ; Models, Biological ; Movement/physiology ; Pilot Projects ; Professional Competence ; Psychomotor Performance/physiology ; Range of Motion, Articular ; Robotics/instrumentation ; Surgery, Computer-Assisted/instrumentation ; User-Computer Interface
Language	English
Publishing date	2013
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	0926-9630
ISSN	0926-9630
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Article ; Online: Ultrasound-assisted emulsification microextraction for determination of 2,4,6-trichloroanisole in wine samples by gas chromatography tandem mass spectrometry.

Fontana, Ariel R / Patil, Sangram H / Banerjee, Kaushik / Altamirano, Jorgelina C

Journal of agricultural and food chemistry

2010 Volume 58, Issue 8, Page(s) 4576–4581

Abstract: A fast and effective microextraction technique is proposed for preconcentration of 2,4,6-trichloroanisole (2,4,6-TCA) from wine samples prior gas chromatography tandem mass spectrometric (GC-MS/MS) analysis. The proposed technique is based on ... ...

Abstract	A fast and effective microextraction technique is proposed for preconcentration of 2,4,6-trichloroanisole (2,4,6-TCA) from wine samples prior gas chromatography tandem mass spectrometric (GC-MS/MS) analysis. The proposed technique is based on ultrasonication (US) for favoring the emulsification phenomenon during the extraction stage. Several variables influencing the relative response of the target analyte were studied and optimized. Under optimal experimental conditions, 2,4,6-TCA was quantitatively extracted achieving enhancement factors (EF) > or = 400 and limits of detection (LODs) 0.6-0.7 ng L(-1) with relative standard deviations (RSDs) < or = 11.3%, when 10 ng L(-1) 2,4,6-TCA standard-wine sample blend was analyzed. The calibration graphs for white and red wine were linear within the range of 5-1000 ng L(-1), and estimation coefficients (r(2)) were > or = 0.9995. Validation of the methodology was carried out by standard addition method at two concentrations (10 and 50 ng L(-1)) achieving recoveries >80% indicating satisfactory robustness of the method. The methodology was successfully applied for determination of 2,4,6-TCA in different wine samples.
MeSH term(s)	Anisoles/analysis ; Emulsions ; Gas Chromatography-Mass Spectrometry/methods ; Solvents ; Tandem Mass Spectrometry/methods ; Temperature ; Ultrasonics ; Wine/analysis
Chemical Substances	Anisoles ; Emulsions ; Solvents ; 2,4,6-trichloroanisole (31O3X41254)
Language	English
Publishing date	2010-04-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/jf904396g
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