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  1. Article ; Online: BiFC Method Based on Intraorganellar Protein Crowding Detects Oleate-Dependent Peroxisomal Targeting of Pichia pastoris Malate Dehydrogenase

    Jean-Claude Farré / Paul Li / Suresh Subramani

    International Journal of Molecular Sciences, Vol 22, Iss 4890, p

    2021  Volume 4890

    Abstract: The maintenance of intracellular NAD + /NADH homeostasis across multiple, subcellular compartments requires the presence of NADH-shuttling proteins, which circumvent the lack of permeability of organelle membranes to these cofactors. Very little is known ...

    Abstract The maintenance of intracellular NAD + /NADH homeostasis across multiple, subcellular compartments requires the presence of NADH-shuttling proteins, which circumvent the lack of permeability of organelle membranes to these cofactors. Very little is known regarding these proteins in the methylotrophic yeast, Pichia pastoris . During the study of the subcellular locations of these shuttling proteins, which often have dual subcellular locations, it became necessary to develop new ways to detect the weak peroxisomal locations of some of these proteins. We have developed a novel variation of the traditional Bimolecular Fluorescence Complementation (BiFC), called divergent BiFC, to detect intraorganellar colocalization of two noninteracting proteins based on their proximity-based protein crowding within a small subcellular compartment, rather than on the traditional protein–protein interactions expected for BiFC. This method is used to demonstrate the partially peroxisomal location of one such P. pastoris NADH-shuttling protein, malate dehydrogenase B, only when cells are grown in oleate, but not when grown in methanol or glucose. We discuss the mode of NADH shuttling in P. pastoris and the physiological basis of the medium-dependent compartmentalization of Pp MdhB.
    Keywords redox balance ; NADH shuttle ; peroxisomal malate dehydrogenase ; environment-dependent peroxisomal targeting ; intraorganellar protein crowding ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 612
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Mitigating the impacts of the COVID-19 pandemic on vulnerable populations: Lessons for improving health and social equity.

    Tan, Si Ying / Foo, Chuan De / Verma, Monica / Hanvoravongchai, Piya / Cheh, Paul Li Jen / Pholpark, Aungsumalee / Marthias, Tiara / Hafidz, Firdaus / Prawidya Putri, Likke / Mahendradhata, Yodi / Giang, Kim Bao / Nachuk, Stefan / Wang, Hong / Lim, Jeremy / Legido-Quigley, Helena

    Social science & medicine (1982)

    2023  Volume 328, Page(s) 116007

    Abstract: The COVID-19 pandemic had an inequitable and disproportionate impact on vulnerable populations, reversing decades of progress toward healthy populations and poverty alleviation. This study examines various programmatic tools and policy measures used by ... ...

    Abstract The COVID-19 pandemic had an inequitable and disproportionate impact on vulnerable populations, reversing decades of progress toward healthy populations and poverty alleviation. This study examines various programmatic tools and policy measures used by governments to support vulnerable populations during the pandemic. A comparative case study of 15 countries representing all World Health Organization's regions offers a comprehensive picture of countries with varying income statuses, health system arrangements and COVID-19 public health measures. Through a systematic desk review and key informant interviews, we report a spectrum of mitigation strategies deployed in these countries to address five major types of vulnerabilities (health, economic, social, institutional and communicative). We found a multitude of strategies that supported vulnerable populations such as migrant workers, sex workers, prisoners, older persons and school-going children. Prioritising vulnerable populations during the early phase of COVID-19 vaccination campaigns, direct financial subsidies and food assistance programmes were the most common measures reported. Additionally, framing public health information and implementing culturally sensitive health promotion interventions helped bridge the communication barriers in certain instances. However, these measures remain insufficient to protect vulnerable populations comprehensively. Our findings point to the need to expand fiscal space for health, enlarge healthcare coverage, incorporate equity principles in all policies, leverage technology, multi-stakeholder co-production of policies and tailored community engagement mechanisms.
    MeSH term(s) Child ; Humans ; Aged ; Aged, 80 and over ; COVID-19/epidemiology ; COVID-19/prevention & control ; Pandemics/prevention & control ; COVID-19 Vaccines ; Poverty ; Public Health ; Vulnerable Populations ; Health Equity
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2023-06-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 4766-1
    ISSN 1873-5347 ; 0037-7856 ; 0277-9536
    ISSN (online) 1873-5347
    ISSN 0037-7856 ; 0277-9536
    DOI 10.1016/j.socscimed.2023.116007
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  3. Article ; Online: Economic Evaluation of Exercise-Based Fall Prevention Programs for People with Parkinson's Disease: A Systematic Review.

    Winser, Stanley John / Paul, Li Francis / Magnus, Leung Ka Long / Yan, Szeto / Shenug, Tsui Pui / Sing, Yuen Man / Cheing, Gladys

    Journal of alternative and complementary medicine (New York, N.Y.)

    2019  Volume 25, Issue 12, Page(s) 1225–1237

    Abstract: Objectives: ...

    Abstract Objectives:
    MeSH term(s) Accidental Falls/prevention & control ; Aged ; Cost-Benefit Analysis ; Exercise Therapy/economics ; Exercise Therapy/methods ; Exercise Therapy/statistics & numerical data ; Female ; Humans ; Male ; Parkinson Disease/therapy ; Tai Ji
    Language English
    Publishing date 2019-09-26
    Publishing country United States
    Document type Journal Article ; Systematic Review
    ZDB-ID 1237383-7
    ISSN 1557-7708 ; 1075-5535
    ISSN (online) 1557-7708
    ISSN 1075-5535
    DOI 10.1089/acm.2019.0148
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  4. Article ; Online: Re: antibiotic prophylaxis for shock wave lithotripsy in patients with sterile urine before treatment may be unnecessary: a systematic review and meta-analysis: Y. Lu, F. Tianyong, H. Ping, L. Liangren, Y. Haichao and W. Qiang J Urol 2012;188:441-448.

    Mosli, Hisham A / Aaronson, David Seth / Forouzeh, Zahra / Paul, Li

    The Journal of urology

    2013  Volume 189, Issue 2, Page(s) 775–777

    MeSH term(s) Antibiotic Prophylaxis ; Humans ; Lithotripsy ; Unnecessary Procedures ; Urine/microbiology
    Language English
    Publishing date 2013-02
    Publishing country United States
    Document type Comment ; Letter
    ZDB-ID 3176-8
    ISSN 1527-3792 ; 0022-5347
    ISSN (online) 1527-3792
    ISSN 0022-5347
    DOI 10.1016/j.juro.2012.08.212
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  5. Article ; Online: Health financing policies during the COVID-19 pandemic and implications for universal health care: a case study of 15 countries.

    De Foo, Chuan / Verma, Monica / Tan, Si Ying / Hamer, Jess / van der Mark, Nina / Pholpark, Aungsumalee / Hanvoravongchai, Piya / Cheh, Paul Li Jen / Marthias, Tiara / Mahendradhata, Yodi / Putri, Likke Prawidya / Hafidz, Firdaus / Giang, Kim Bao / Khuc, Thi Hong Hanh / Van Minh, Hoang / Wu, Shishi / Caamal-Olvera, Cinthya G / Orive, Gorka / Wang, Hong /
    Nachuk, Stefan / Lim, Jeremy / de Oliveira Cruz, Valeria / Yates, Rob / Legido-Quigley, Helena

    The Lancet. Global health

    2023  Volume 11, Issue 12, Page(s) e1964–e1977

    Abstract: Background: The COVID-19 pandemic was a health emergency requiring rapid fiscal resource mobilisation to support national responses. The use of effective health financing mechanisms and policies, or lack thereof, affected the impact of the pandemic on ... ...

    Abstract Background: The COVID-19 pandemic was a health emergency requiring rapid fiscal resource mobilisation to support national responses. The use of effective health financing mechanisms and policies, or lack thereof, affected the impact of the pandemic on the population, particularly vulnerable groups and individuals. We provide an overview and illustrative examples of health financing policies adopted in 15 countries during the pandemic, develop a framework for resilient health financing, and use this pandemic to argue a case to move towards universal health coverage (UHC).
    Methods: In this case study, we examined the national health financing policy responses of 15 countries, which were purposefully selected countries to represent all WHO regions and have a range of income levels, UHC index scores, and health system typologies. We did a systematic literature review of peer-reviewed articles, policy documents, technical reports, and publicly available data on policy measures undertaken in response to the pandemic and complemented the data obtained with 61 in-depth interviews with health systems and health financing experts. We did a thematic analysis of our data and organised key themes into a conceptual framework for resilient health financing.
    Findings: Resilient health financing for health emergencies is characterised by two main phases: (1) absorb and recover, where health systems are required to absorb the initial and subsequent shocks brought about by the pandemic and restabilise from them; and (2) sustain, where health systems need to expand and maintain fiscal space for health to move towards UHC while building on resilient health financing structures that can better prepare health systems for future health emergencies. We observed that five key financing policies were implemented across the countries-namely, use of extra-budgetary funds for a swift initial response, repurposing of existing funds, efficient fund disbursement mechanisms to ensure rapid channelisation to the intended personnel and general population, mobilisation of the private sector to mitigate the gaps in public settings, and expansion of service coverage to enhance the protection of vulnerable groups. Accountability and monitoring are needed at every stage to ensure efficient and accountable movement and use of funds, which can be achieved through strong governance and coordination, information technology, and community engagement.
    Interpretation: Our findings suggest that health systems need to leverage the COVID-19 pandemic as a window of opportunity to make health financing policies robust and need to politically commit to public financing mechanisms that work to prepare for future emergencies and as a lever for UHC.
    Funding: Bill & Melinda Gates Foundation.
    MeSH term(s) Humans ; Pandemics ; Healthcare Financing ; Universal Health Care ; Emergencies ; COVID-19/epidemiology ; Health Policy
    Language English
    Publishing date 2023-11-14
    Publishing country England
    Document type Systematic Review ; Journal Article
    ZDB-ID 2723488-5
    ISSN 2214-109X ; 2214-109X
    ISSN (online) 2214-109X
    ISSN 2214-109X
    DOI 10.1016/S2214-109X(23)00448-5
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  6. Article: The price of sin in the Pacific-Basin

    Durand, Robert B / Koh, SzeKee / Tan, Paul LiJian

    Pacific-Basin finance journal Vol. 21, No. 1 , p. 899-913

    2013  Volume 21, Issue 1, Page(s) 899–913

    Author's details Robert B. Durand; SzeKee Koh; Paul LiJian Tan
    Language Undetermined
    Size graph. Darst.
    Publisher Elsevier
    Publishing place Amsterdam [u.a.]
    Document type Article
    ZDB-ID 1343420-2
    ISSN 0927-538X
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  7. Book ; Online ; Thesis: Analyse von Gen- und Proteinexpressionsprofilen an Zellen des Unterhautfettgewebes in Assoziation mit postoperativen Wundheilungsstörungen

    Paul, Laura Isabelle [Verfasser] / Gillen, Sonja [Akademischer Betreuer] / Kleeff, Jörg H. [Akademischer Betreuer] / Friess, Helmut [Akademischer Betreuer]

    2015  

    Author's details Laura Isabelle Paul. Betreuer: Sonja Gillen. Gutachter: Jörg H. Kleeff ; Helmut Friess
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Universitätsbibliothek der TU München
    Publishing place München
    Document type Book ; Online ; Thesis
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  8. Article: Carbohydrate fatty acid monosulphate esters are safe and effective adjuvants for humoral responses

    Hilgers, Luuk A.Th / Geert van Amerongen / Jeffrey Bajramovic / Jennifer Veth / Johannes F. van den Bosch / Koert J. Stittelaar / Marie Pohl / Peter Paul L.I. Platenburg / Robert Sauerwein / Will Roeffen

    Vaccine. 2017 May 31, v. 35, no. 24

    2017  

    Abstract: Carbohydrate fatty acid sulphate esters (CFASEs) formulated in a squalane-in-water emulsion are effective adjuvants for humoral responses to a wide range of antigens in various animal species but rise in body temperature and local reactions albeit mild ... ...

    Abstract Carbohydrate fatty acid sulphate esters (CFASEs) formulated in a squalane-in-water emulsion are effective adjuvants for humoral responses to a wide range of antigens in various animal species but rise in body temperature and local reactions albeit mild or minimal hampers application in humans. In rabbits, body temperature increased 1°C one day after intramuscular (IM) injection, which returned to normal during the next day. The effect increased with increasing dose of CFASE but not with the number of injections (up to 5). Antigen enhanced the rise in body temperature after booster immunization (P<0.01) but not after priming. Synthetic CFASEs are mixtures of derivatives containing no sulphate, one or multiple sulphate groups and the monosulphate derivatives (CMS) were isolated, incorporated in a squalane in-water emulsion and investigated. In contrast to CFASE, CMS adjuvant did not generate rise in body temperature or local reactions in rabbits immunized with a purified, recombinant malaria chimeric antigen R0.10C. In comparison to alum, CMS adjuvant revealed approximately 30-fold higher antibody titres after the first and >100-fold after the second immunization. In ferrets immunized with 7.5μg of inactivated influenza virus A/H7N9, CMS adjuvant gave 100-fold increase in HAI antibody titres after the first and 25-fold after the second immunisation, which were 10–20-fold higher than with the MF59-like AddaVax adjuvant. In both models, a single immunisation with CMS adjuvant revealed similar or higher titres than two immunisations with either benchmark, without detectable systemic and local adverse effects. Despite striking chemical similarities with monophospholipid A (MPL), CMS adjuvant did not activate human TLR4 expressed on HEK cells. We concluded that the synthetic CMS adjuvant is a promising candidate for poor immunogens and single-shot vaccines and that rise in body temperature, local reactions or activation of TLR4 is not a pre-requisite for high adjuvanticity.
    Keywords adjuvants ; adverse effects ; alum ; antibodies ; antigens ; body temperature ; chimerism ; emulsions ; esters ; fatty acids ; ferrets ; humans ; humoral immunity ; malaria ; models ; Orthomyxoviridae ; rabbits ; secondary immunization ; Toll-like receptor 4 ; vaccines
    Language English
    Dates of publication 2017-0531
    Size p. 3249-3255.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2017.04.055
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  9. Article ; Online: Genome Sequence of Anoxybacillus geothermalis Strain GSsed3, a Novel Thermophilic Endospore-Forming Species

    Sevasti Filippidou / Marion Jaussi / Thomas Junier / Tina Wunderlin / Ludovic Roussel-Delif / Nicole Jeanneret / A. Vieth-Hillebrand / A. Vetter / Simona Regenspurg / Shannon L. Johnson / Kim McMurry / Cheryl D. Gleasner / Chien-Chi Lo / Paul Li / Momchilo Vuyisich / Patrick S. Chain / Pilar Junier

    Genome Announcements

    2015  

    Publishing country de
    Document type Article ; Online
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  10. Article: Paraneoplastic Cerebellar Degeneration: Neurological Symptoms Pointing to Occult Ovarian Cancer

    Geomini, Peggy M.A.J. / Dellemijn, Paul L.I. / Bremer, Gérard L.

    Gynecologic and Obstetric Investigation

    2001  Volume 52, Issue 2, Page(s) 145–146

    Abstract: Paraneoplastic cerebellar degeneration with anti-Yo antibodies is a rare but disabling neurodegenerative disease that may point to an occult ovarian cancer. Symptoms usually accompanying paraneoplastic cerebellar degeneration include truncal and limb ... ...

    Institution Departments of Obstetrics and Gynecology and Neurology, Saint Joseph Hospital, Veldhoven, The Netherlands
    Abstract Paraneoplastic cerebellar degeneration with anti-Yo antibodies is a rare but disabling neurodegenerative disease that may point to an occult ovarian cancer. Symptoms usually accompanying paraneoplastic cerebellar degeneration include truncal and limb ataxia, dysarthria, dysphagia, nystagmus, vertigo, and diplopia. The pathogenesis of paraneoplastic neurological syndromes is unknown. Treatment results of the neurological symptoms are disappointing. The present case illustrates how neurological symptoms pointed to an occult ovarian cancer.
    Keywords Paraneoplastic cerebellar syndrome ; Ovarian cancer ; Antineuronal antibodies
    Language English
    Publishing date 2001-09-28
    Publisher S. Karger AG
    Publishing place Basel, Switzerland
    Document type Article
    Note Case Report
    ZDB-ID 800003-7
    ISSN 1423-002X ; 0378-7346
    ISSN (online) 1423-002X
    ISSN 0378-7346
    DOI 10.1159/000052961
    Database Karger publisher's database

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