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Article ; Online: Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome.

Apostolovic, Danijela / Grundström, Jeanette / Kiewiet, Mensiena B Gea / Perusko, Marija / Hamsten, Carl / Starkhammar, Maria / Paulie, Staffan / van Hage, Marianne

The Journal of clinical investigation

2023 Volume 133, Issue 6

Abstract: Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated ... ...

Abstract	Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cytokine profiles in response to tick protein extract (TE) and α-Gal-free TE in patients with AGS and in healthy controls. T and B cells from both patients and controls proliferated in response to TE, but significantly more in patients with AGS. B cell proliferation, but not T cell proliferation, in patients with AGS was reduced by removing α-Gal from the TE. In addition, TE induced a clear Th2 cytokine profile in patients with AGS. Expression of CD23 by B cells correlated only to T cell proliferation. However, both B cell proliferation and CD23 expression were reduced when CD40L and IL-4 were blocked. A large portion of the IgG1 and IgE antibodies binding TE in patients with AGS were directed against the α-Gal epitope. We have, for what we believe to be the first time, investigated T and B cell responses to α-Gal carrying tick proteins in patients with AGS, which will be essential for the understanding of the immune response against an allergenic carbohydrate transmitted by ticks.
MeSH term(s)	Animals ; Humans ; Ticks ; Galactose ; Immunoglobulin E ; Food Hypersensitivity ; Allergens ; Cytokines
Chemical Substances	Galactose (X2RN3Q8DNE) ; Immunoglobulin E (37341-29-0) ; Allergens ; Cytokines
Language	English
Publishing date	2023-03-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3067-3
ISSN	1558-8238 ; 0021-9738
ISSN (online)	1558-8238
ISSN	0021-9738
DOI	10.1172/JCI158357
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Article ; Online: A novel adjuvant G3 induces both Th1 and Th2 related immune responses in mice after immunization with a trivalent inactivated split-virion influenza vaccine.

Hjertner, Bernt / Bengtsson, Theresa / Morein, Bror / Paulie, Staffan / Fossum, Caroline

Vaccine

2018 Volume 36, Issue 23, Page(s) 3340–3344

Abstract: A preferred adjuvant should promote both Th1 and Th2 responses. However, most adjuvants in common use are biased towards a Th2-driven response. Therefore, the ability of a novel saponin-based adjuvant G3 to inducing balanced Th1 and Th2 responses in BALB/ ...

Abstract	A preferred adjuvant should promote both Th1 and Th2 responses. However, most adjuvants in common use are biased towards a Th2-driven response. Therefore, the ability of a novel saponin-based adjuvant G3 to inducing balanced Th1 and Th2 responses in BALB/c mice immunized with a split trivalent seasonal influenza vaccine was evaluated in comparison to that of the adjuvant Al(OH)
MeSH term(s)	Adjuvants, Immunologic/pharmacology ; Aluminum Hydroxide/immunology ; Aluminum Hydroxide/pharmacology ; Animals ; Cytokines/immunology ; Cytokines/metabolism ; Immunoglobulin G/blood ; Influenza Vaccines/immunology ; Mice, Inbred BALB C ; Spleen/cytology ; Spleen/immunology ; Th1 Cells/drug effects ; Th1 Cells/immunology ; Th2 Cells/drug effects ; Th2 Cells/immunology ; Virion/immunology
Chemical Substances	Adjuvants, Immunologic ; Cytokines ; Immunoglobulin G ; Influenza Vaccines ; Aluminum Hydroxide (5QB0T2IUN0)
Language	English
Publishing date	2018-04-26
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2018.04.054
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Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: A novel adjuvant G3 induces both Th1 and Th2 related immune responses in mice after immunization with a trivalent inactivated split-virion influenza vaccine

Hjertner, Bernt / Bengtsson, Theresa / Fossum, Caroline / Morein, Bror / Paulie, Staffan

Vaccine. 2018 May 31, v. 36, no. 23

2018

Abstract	A preferred adjuvant should promote both Th1 and Th2 responses. However, most adjuvants in common use are biased towards a Th2-driven response. Therefore, the ability of a novel saponin-based adjuvant G3 to inducing balanced Th1 and Th2 responses in BALB/c mice immunized with a split trivalent seasonal influenza vaccine was evaluated in comparison to that of the adjuvant Al(OH)3. Clear differences in the IgG profiles induced by G3, Al(OH)3 or non-adjuvanted vaccine were recorded. Both adjuvants enhanced high and similar levels of the Th2 associated IgG1 subtype compared to mice given vaccine alone. Only G3 enhanced the IgG2a subclass reflecting a Th1 response, whereas Al(OH)3 even abrogated the IgG2a production. Accordingly, G3 enhanced the production of IL-2 and IFN-γ and also of IL-2/IFN-γ double secreting cells, emphasizing the strong Th1 driving effect of G3. Only Al(OH)3 increased splenocyte production of IL-17. Taken together, the results indicate a strong propensity for G3 to induce both Th1 and Th2 driven immune responses.
Keywords	adjuvants ; aluminum hydroxide ; immune response ; immunization ; immunoglobulin G ; influenza vaccines ; interferon-gamma ; interleukin-17 ; interleukin-2 ; mice ; splenocytes
Language	English
Dates of publication	2018-0531
Size	p. 3340-3344.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2018.04.054
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Article ; Online: Intensive weight gain therapy in patients with anorexia nervosa results in improved serum tartrate-resistant acid phosphatase (TRAP) 5a and 5b isoform protein levels.

Patlaka, Christina / Tubic, Bojan / Lång, Pernilla / Paulie, Staffan / Swolin-Eide, Diana / Magnusson, Per / Andersson, Göran

Eating and weight disorders : EWD

2019 Volume 25, Issue 5, Page(s) 1387–1397

Abstract: Aim: Tartrate-resistant acid phosphatase (TRAP) exists as isoforms 5a and 5b. TRAP 5a is a biomarker of chronic inflammation and influences adipose tissue and 5b associates with bone metabolism/pathologies. The aim was to investigate the association of ... ...

Abstract	Aim: Tartrate-resistant acid phosphatase (TRAP) exists as isoforms 5a and 5b. TRAP 5a is a biomarker of chronic inflammation and influences adipose tissue and 5b associates with bone metabolism/pathologies. The aim was to investigate the association of serum TRAP 5a/5b isoforms with fat and bone markers and anthropometric parameters in patients with anorexia nervosa (AN) during weight gain therapy. Methods: Twenty-five Swedish female AN patients, age 16-24 years, were treated for 12 weeks with a high-energy diet with six meals daily. Serum TRAP 5a/5b, markers of fat/glucose metabolism, markers of bone resorption and formation were measured. Parameters of bone and body composition were assessed by dual-energy X-ray absorptiometry and peripheral quantitative computed tomography. Results: BMI increased from median 15.4 kg/m Conclusions: This clinical interventional study resulted in increased BMI in patients with AN. The decreased TRAP 5b protein levels confirm a role for TRAP 5b as a marker of bone resorption, whereas increased TRAP 5a seemed to derive from systemic changes in bone as well as metabolic changes. The combined detection of TRAP 5a and TRAP 5b in serum could be an indicator of improved bone metabolism. Level of evidence: Level III, prospective interventional cohort study.
MeSH term(s)	Adolescent ; Adult ; Anorexia Nervosa/therapy ; Biomarkers ; Cohort Studies ; Female ; Humans ; Isoenzymes ; Prospective Studies ; Tartrate-Resistant Acid Phosphatase/blood ; Weight Gain ; Young Adult
Chemical Substances	Biomarkers ; Isoenzymes ; ACP5 protein, human (EC 3.1.3.2) ; Tartrate-Resistant Acid Phosphatase (EC 3.1.3.2)
Language	English
Publishing date	2019-09-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2038625-4
ISSN	1590-1262 ; 1124-4909
ISSN (online)	1590-1262
ISSN	1124-4909
DOI	10.1007/s40519-019-00776-8
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Zs.A 5566: Show issues

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Article ; Online: ELISpot and ELISA analyses of human IL-21-secreting cells: Impact of blocking IL-21 interaction with cellular receptors.

Huang, Jenny / Ehrnfelt, Cecilia / Paulie, Staffan / Zuber, Bartek / Ahlborg, Niklas

Journal of immunological methods

2015 Volume 417, Page(s) 60–66

Abstract: Interleukin (IL)-21 is crucial for the regulation of lymphocytes and is implicated in autoimmune and other diseases. The relevance of being able to measure human IL-21 prompted us to develop ELISA and ELISpot assays for analysis of IL-21 levels and IL-21- ...

Abstract	Interleukin (IL)-21 is crucial for the regulation of lymphocytes and is implicated in autoimmune and other diseases. The relevance of being able to measure human IL-21 prompted us to develop ELISA and ELISpot assays for analysis of IL-21 levels and IL-21-producing cells, respectively. Monoclonal antibodies (mAbs) to IL-21 were made and ELISA and ELISpot assays were developed. The selected detection mAb also neutralized IL-21-mediated activation of human cells. Peripheral blood mononuclear cells (PBMCs) from healthy donors (n=24) were stimulated polyclonally (phytohemagglutinin; PHA) or with antigen (Candida albicans extract and tetanus toxoid). Using ELISpot, high numbers of IL-21-producing cells were detected after PHA activation; lower but positive responses to antigen were seen in approximately 50% of the donors. In contrast, the ELISA detected IL-21 in supernatants from PHA-activated cells but not from antigen-stimulated cells. When analyzing IL-17A in parallel, PHA and antigens induced detectable responses in ELISpot as well as in ELISA. Hypothesizing that the lack of detectable IL-21 levels after antigenic stimulation was due to a combination of low frequencies of IL-21-secreting cells and consumption of IL-21 by cellular receptors during cell culture, PBMCs (n=18) were stimulated in the presence of the neutralizing detection mAb. When preventing IL-21 from interacting with its receptor, increased IL-21 levels were found by ELISA after PHA activation and IL-21 could also be measured after antigen stimulation. ELISpot results were unaffected by the addition of the neutralizing mAb. In conclusion, IL-21 secreted by low frequencies of antigen-specific ex vivo-stimulated PBMC can be difficult to detect by ELISA but prevention of IL-21 interaction with its receptor leads to detectable IL-21 levels. In ELISpot, where the cytokine is captured by mAbs on a solid phase immediately upon secretion, blocking the receptor interaction does not affect the detection of IL-21-secreting cells.
MeSH term(s)	Antibodies, Monoclonal/immunology ; Candida albicans/immunology ; Cell Line ; Enzyme-Linked Immunosorbent Assay ; Enzyme-Linked Immunospot Assay ; HEK293 Cells ; Humans ; Interleukin-17/immunology ; Interleukin-17/secretion ; Interleukins/analysis ; Interleukins/immunology ; Leukocytes, Mononuclear/immunology ; Phytohemagglutinins/immunology ; Receptors, Interleukin-21/antagonists & inhibitors ; T-Lymphocytes, Helper-Inducer/immunology ; Tetanus Toxoid/immunology
Chemical Substances	Antibodies, Monoclonal ; IL17A protein, human ; Interleukin-17 ; Interleukins ; Phytohemagglutinins ; Receptors, Interleukin-21 ; Tetanus Toxoid ; interleukin-21
Language	English
Publishing date	2015-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	120142-6
ISSN	1872-7905 ; 0022-1759
ISSN (online)	1872-7905
ISSN	0022-1759
DOI	10.1016/j.jim.2014.12.007
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Zs.A 795: Show issues

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Article ; Online: A Novel Sandwich ELISA for Tartrate-Resistant Acid Phosphatase 5a and 5b Protein Reveals that Both Isoforms are Secreted by Differentiating Osteoclasts and Correlate to the Type I Collagen Degradation Marker CTX-I In Vivo and In Vitro.

Mira-Pascual, Laia / Patlaka, Christina / Desai, Suchita / Paulie, Staffan / Näreoja, Tuomas / Lång, Pernilla / Andersson, Göran

Calcified tissue international

2019 Volume 106, Issue 2, Page(s) 194–207

Abstract: Tartrate-resistant acid phosphatase type 5 (TRAP) exists as two isoforms, 5a and 5b. 5b is a marker of osteoclast number and 5a of chronic inflammation; however, its association with bone resorption is unknown. In this study, a double-TRAP 5a/5b sandwich ...

Abstract	Tartrate-resistant acid phosphatase type 5 (TRAP) exists as two isoforms, 5a and 5b. 5b is a marker of osteoclast number and 5a of chronic inflammation; however, its association with bone resorption is unknown. In this study, a double-TRAP 5a/5b sandwich ELISA measuring 5a and 5b protein in the same sample was developed. TRAP 5a and 5b protein levels were evaluated as osteoclast differentiation/activity markers in serum and in culture, and their correlation to the resorption marker CTX-I was examined. Serum TRAP 5a and 5b concentrations in healthy men were 4.4 ± 0.6 ng/ml and 1.3 ± 0.2 ng/ml, respectively, and they correlated moderately to each other suggesting that their secretion is coupled under healthy conditions. A correlation was also observed between serum TRAP 5a and 5b with CTX-I, suggesting that both TRAP isoforms associate with osteoclast number. During osteoclast differentiation on plastic/bone, predominantly 5b increased in media/lysate from M-CSF/RANKL-stimulated CD14+ PBMCs. However, substantial levels of 5a were detected at later stages suggesting that both isoforms are secreted from differentiating OCs. More TRAP 5b was released on bone indicating a connection to osteoclast resorptive activity, and a peak in TRAP 5b/5a-ratio coincided with rapid CTX-I release. At the end of the culture period of M-CSF + RANKL-stimulated CD14+ PBMCs, there was a correlation between the secretion of TRAP 5a and 5b proteins with CTX-I. The correlation of not only 5b but also 5a with collagen degradation, both in serum and osteoclast cultures indicates that a considerable proportion of the TRAP 5a originates from osteoclasts and may reflect a hitherto undisclosed regulatory mechanism during bone resorption and bone remodeling.
MeSH term(s)	Adult ; Aged ; Biomarkers/analysis ; Biomarkers/metabolism ; Cells, Cultured ; Collagen Type I/metabolism ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Isoenzymes/analysis ; Isoenzymes/metabolism ; Male ; Middle Aged ; Osteoclasts/metabolism ; Peptides/metabolism ; Proteolysis ; Secretory Pathway ; Tartrate-Resistant Acid Phosphatase/analysis ; Tartrate-Resistant Acid Phosphatase/metabolism
Chemical Substances	Biomarkers ; Collagen Type I ; Isoenzymes ; Peptides ; collagen type I trimeric cross-linked peptide ; ACP5 protein, human (EC 3.1.3.2) ; Tartrate-Resistant Acid Phosphatase (EC 3.1.3.2)
Language	English
Publishing date	2019-10-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	304266-2
ISSN	1432-0827 ; 0944-0747 ; 0008-0594 ; 0171-967X
ISSN (online)	1432-0827
ISSN	0944-0747 ; 0008-0594 ; 0171-967X
DOI	10.1007/s00223-019-00618-w
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Article ; Online: ApoD mediates binding of HDL to LDL and to growing T24 carcinoma.

Braesch-Andersen, Sten / Beckman, Lena / Paulie, Staffan / Kumagai-Braesch, Makiko

PloS one

2014 Volume 9, Issue 12, Page(s) e115180

Abstract: Apolipoprotein (Apo) D is an important protein produced in many parts of the body. It is necessary for the development and repair of the brain and protection from oxidative stress. The purpose of this study was to investigate the extent to which apoD ... ...

Abstract	Apolipoprotein (Apo) D is an important protein produced in many parts of the body. It is necessary for the development and repair of the brain and protection from oxidative stress. The purpose of this study was to investigate the extent to which apoD interacts with lipoproteins in human plasma. By using detergent-free ELISA, we show that immobilized monoclonal antibodies against apoD very efficiently bind to low density lipoprotein (LDL) from plasma; this binding is as equally efficient as binding to an anti-apoB monoclonal antibody. Adding detergent to the plasma inhibited the binding, suggesting that the binding is dependent on the presence of intact lipoprotein particles. Reversing the system by using immobilized anti-apoB revealed that the affinity of apoD for LDL is rather low, suggesting that multiple bindings are needed for a durable connection. Biosensor experiments using purified lipoproteins also showed that purified apoD and high density lipoprotein 3 (HDL3), a lipoprotein fraction rich in apoD, were both able to bind LDL very efficiently, indicating that the HDL3-LDL interaction may be a physiological consequence of the affinity of apoD for LDL. Furthermore, we found that apoD increases the binding of HDL to actively growing T24 bladder carcinoma cells but not to quiescent, contact-inhibited, confluent T24 cells. This result is especially intriguing given that the T24 supernatant only contained detectable levels of apoD after growth inhibition, raising the possibility that alternating the expression of apoD and a putative apoD-receptor could give direction to the flow of lipids. In the current paper, we conclude that apoD mediates binding of HDL to LDL and to growing T24 carcinomas, thereby highlighting the importance of apoD in lipid metabolism.
MeSH term(s)	Antibodies, Monoclonal/immunology ; Apolipoproteins D/blood ; Apolipoproteins D/immunology ; Apolipoproteins D/metabolism ; Carcinoma/metabolism ; Cell Line, Tumor ; Enzyme-Linked Immunosorbent Assay ; Humans ; Lipoproteins, HDL/blood ; Lipoproteins, HDL/metabolism ; Lipoproteins, LDL/blood ; Lipoproteins, LDL/metabolism ; Protein Binding ; Urinary Bladder Neoplasms/metabolism
Chemical Substances	APOD protein, human ; Antibodies, Monoclonal ; Apolipoproteins D ; Lipoproteins, HDL ; Lipoproteins, LDL
Keywords	covid19
Language	English
Publishing date	2014
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0115180
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Article ; Online: The adipokine tartrate-resistant acid phosphatase 5a in serum correlates to adipose tissue expansion in obesity.

Patlaka, Christina / Mira Pascual, Laia / Paulie, Staffan / Henriksson, Anni-Frid / Arner, Peter / Lång, Pernilla / Andersson, Göran

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals

2017 Volume 22, Issue 8, Page(s) 764–774

Abstract: Purpose: Tartrate-resistant acid phosphatase (TRAP) exists as two isoforms, 5a and 5b. TRAP 5a is elevated in adipose tissue of obese women, interacts with pre-adipocytes and is linked to insulin-sensitive hyperplastic obesity when overexpressed in mice. ...

Abstract	Purpose: Tartrate-resistant acid phosphatase (TRAP) exists as two isoforms, 5a and 5b. TRAP 5a is elevated in adipose tissue of obese women, interacts with pre-adipocytes and is linked to insulin-sensitive hyperplastic obesity when overexpressed in mice. The aim of this study was to investigate the relation between serum TRAP 5a, adiposity indices and metabolic syndrome risk markers in lean and obese women, using a newly developed TRAP 5a-specific ELISA. Materials and methods: A TRAP 5a sandwich ELISA was optimized using TRAP 5a-specific monoclonal antibodies and tested in sera of healthy males. TRAP 5a levels were quantitated in sera from healthy males and lean and obese women. Results: Serum TRAP 5a protein levels were lower in obese women in comparison with lean. In obese, but not in lean women, serum TRAP 5a correlated positively to % fat mass, BMI, waist- and hip circumference, waist-to-hip ratio and PAI, while no correlations to serum leptin, HOMA, glucose, insulin, FFA, HDL, TG, APO-A1 and APO-B were observed. Conclusions: TRAP 5a serum levels correlated positively to anthropometric obesity parameters but not to metabolic syndrome risk factors, indicating that serum TRAP 5a is associated with pathological adipose tissue expansion.
MeSH term(s)	Adipokines/metabolism ; Adipose Tissue/metabolism ; Adiposity ; Adult ; Biomarkers/blood ; Biomarkers/metabolism ; Body Mass Index ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Male ; Obesity/blood ; Obesity/metabolism ; Tartrate-Resistant Acid Phosphatase/blood ; Tartrate-Resistant Acid Phosphatase/metabolism
Chemical Substances	Adipokines ; Biomarkers ; ACP5 protein, human (EC 3.1.3.2) ; Tartrate-Resistant Acid Phosphatase (EC 3.1.3.2)
Language	English
Publishing date	2017-12
Publishing country	England
Document type	Journal Article
ZDB-ID	1324372-x
ISSN	1366-5804 ; 1354-750X
ISSN (online)	1366-5804
ISSN	1354-750X
DOI	10.1080/1354750X.2017.1334155
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Zs.A 4864: Show issues

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Article: Caveolae-mediated endocytosis of the glucosaminoglycan-interacting adipokine tartrate resistant acid phosphatase 5a in adipocyte progenitor lineage cells.

Patlaka, Christina / Norgård, Maria / Paulie, Staffan / Nordvall-Bodell, Annica / Lång, Pernilla / Andersson, Göran

Biochimica et biophysica acta

2014 Volume 1843, Issue 3, Page(s) 495–507

Abstract: Adipogenesis depends on growth factors controlling proliferation/differentiation of mesenchymal stem cells (MSCs). Membrane binding and endocytosis of growth factors are often coupled to receptor activation and downstream signaling leading to specific ... ...

Abstract	Adipogenesis depends on growth factors controlling proliferation/differentiation of mesenchymal stem cells (MSCs). Membrane binding and endocytosis of growth factors are often coupled to receptor activation and downstream signaling leading to specific cellular responses. The novel adipokine tartrate-resistant acid phosphatase (TRAP) 5a exhibits a growth factor-like effect on MSCs and pre-adipocytes and induces hyperplastic obesity in vivo. However its molecular interaction with pre-adipocytes remains unknown. Therefore, this study aimed to investigate membrane interaction of TRAP and its endocytosis routes in pre-adipocytes. Confocal and/or electron microscopy were used to detect TRAP in untreated or TRAP 5a/b treated pre-adipocytes under conditions that allow or inhibit endocytosis in combination with co-staining of endocytotic vesicles. TRAP interaction with heparin/heparan sulfate was verified by gel filtration. It could be shown that TRAP 5a, but not 5b, binds to the membrane of pre-adipocytes where it co-localizes with heparin-sulfate proteoglycan glypican-4. Also in vitro, TRAP 5a exhibited affinity for both heparin and heparan sulfate with heparin inhibiting its enzyme activity. Upon caveolae-mediated endocytosis of saturating levels of TRAP 5a, TRAP 5a co-localized intracellularly with glypican-4 and late endosomal marker Rab-7 positive vesicles. The protein was also located in multivesicular bodies (MVBs) but did not co-localize with lysosomal marker LAMP-1. TRAP 5a endocytosis was also detectable in pre-osteoblasts, but not fibroblasts, embryonic MSCs or mature adipocytes. These results indicate that TRAP 5a exhibits binding to cell surface, endocytosis and affinity to glucosaminoglycans (GAGs) in pre-adipocyte and pre-osteoblast lineage cells in a manner similar to other heparin-binding growth factors.
MeSH term(s)	3T3 Cells ; Adipocytes/cytology ; Adipocytes/metabolism ; Animals ; Biological Transport ; Caveolae/metabolism ; Cell Line ; Cell Lineage ; Endocytosis/physiology ; Glypicans/metabolism ; Heparin/metabolism ; Heparitin Sulfate/metabolism ; Humans ; Lysosomal-Associated Membrane Protein 1/metabolism ; Membrane Proteins/metabolism ; Mice ; Osteoblasts/metabolism ; Protein Binding/physiology ; Receptors, Thrombin/metabolism ; Stem Cells/cytology ; Stem Cells/metabolism ; rab GTP-Binding Proteins/metabolism
Chemical Substances	Glypicans ; Lysosomal-Associated Membrane Protein 1 ; Membrane Proteins ; Receptors, Thrombin ; rab7 protein (152989-05-4) ; Heparin (9005-49-6) ; Heparitin Sulfate (9050-30-0) ; rab GTP-Binding Proteins (EC 3.6.5.2)
Language	English
Publishing date	2014-03
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbamcr.2013.11.020
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Article ; Online: Activation of Neutrophils via IP3 Pathway Following Exposure to Demodex-Associated Bacterial Proteins.

McMahon, Fred / Banville, Nessa / Bergin, David A / Smedman, Christian / Paulie, Staffan / Reeves, Emer / Kavanagh, Kevin

Inflammation

2016 Volume 39, Issue 1, Page(s) 425–433

Abstract: Rosacea is a chronic inflammatory condition that predominantly affects the skin of the face. Sera from rosacea patients display elevated reactivity to proteins from a bacterium (Bacillus oleronius) originally isolated from a Demodex mite from a rosacea ... ...

Abstract	Rosacea is a chronic inflammatory condition that predominantly affects the skin of the face. Sera from rosacea patients display elevated reactivity to proteins from a bacterium (Bacillus oleronius) originally isolated from a Demodex mite from a rosacea patient suggesting a possible role for bacteria in the induction and persistence of this condition. This work investigated the ability of B. oleronius proteins to activate neutrophils and demonstrated activation via the IP3 pathway. Activated neutrophils displayed increased levels of IP1 production, F-actin formation, chemotaxis, and production of the pro-inflammatory cytokines IL-1β and IL-6 following stimulation by pure and crude B. oleronius protein preparations (2 μg/ml), respectively. In addition, neutrophils exposed to pure and crude B. oleronius proteins (2 μg/ml) demonstrated increased release of internally stored calcium (Ca(2+)), a hallmark of the IP3 pathway of neutrophil activation. Neutrophils play a significant role in the inflammation associated with rosacea, and this work demonstrates how B. oleronius proteins can induce neutrophil recruitment and activation.
MeSH term(s)	Animals ; Bacillus/immunology ; Bacterial Proteins/immunology ; Calcium/metabolism ; Humans ; Inositol 1,4,5-Trisphosphate/metabolism ; Inositol Phosphates/metabolism ; Interleukin-1beta/immunology ; Interleukin-6/immunology ; Mites/microbiology ; Neutrophil Activation/immunology ; Neutrophil Infiltration/immunology ; Neutrophils/immunology ; Rosacea/immunology ; Rosacea/microbiology ; Skin/microbiology ; Skin/pathology
Chemical Substances	Bacterial Proteins ; IL1B protein, human ; IL6 protein, human ; Inositol Phosphates ; Interleukin-1beta ; Interleukin-6 ; inositol 1-phosphate (15421-51-9) ; Inositol 1,4,5-Trisphosphate (85166-31-0) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2016-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	434408-x
ISSN	1573-2576 ; 0360-3997
ISSN (online)	1573-2576
ISSN	0360-3997
DOI	10.1007/s10753-015-0264-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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