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Book ; Online ; E-Book: Handbook of neurophotonics

Pavone, Francesco S. / Shoham, Shy

(Series in cellular and clinical imaging)

2020

Author's details	edited by Francesco S. Pavone, Shy Shoham
Series title	Series in cellular and clinical imaging
Keywords	Electronic books
Language	English
Size	1 Online-Ressource (xviii, 559 Seiten), Illustrationen, Diagramme
Edition	First edition
Publisher	CRC Press
Publishing place	Boca Raton
Publishing country	United States
Document type	Book ; Online ; E-Book
Remark	Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
HBZ-ID	HT020469862
ISBN	978-1-4987-1876-9 ; 978-0-429-19470-2 ; 9781498718752 ; 1-4987-1876-0 ; 0-429-19470-6 ; 1498718752
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: In Vivo Imaging of the Structural Plasticity of Cortical Neurons After Stroke.

Conti, Emilia / Pavone, Francesco Saverio / Allegra Mascaro, Anna Letizia

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2616, Page(s) 69–81

Abstract: The comprehension of the finest mechanisms underlying experience-dependent plasticity requires the investigation of neurons and synaptic terminals in the intact brain over prolonged periods of time. Longitudinal two-photon imaging together with the ... ...

Abstract	The comprehension of the finest mechanisms underlying experience-dependent plasticity requires the investigation of neurons and synaptic terminals in the intact brain over prolonged periods of time. Longitudinal two-photon imaging together with the expression of fluorescent proteins enables high-resolution imaging of dendritic spines and axonal varicosities of cortical neurons in vivo. Importantly, the study of the mechanisms of structural reorganization is relevant for a deeper understanding of the pathophysiological mechanisms of neurological diseases such as stroke and for the development of new therapeutic approaches. This protocol describes the principal steps for in vivo investigation of neuronal plasticity both in healthy conditions and after an ischemic lesion. First, we give a description of the surgery to perform a stable cranial window that allows optical access to the mouse brain cortex. Then we explain how to perform longitudinal two-photon imaging of dendrites, axonal branches, and synaptic terminals in the mouse brain cortex in vivo, in order to investigate the plasticity of synaptic terminals and orientation of neuronal processes. Finally, we describe how to induce an ischemic lesion in a target region of the mouse brain cortex through a cranial window by applying the photothrombotic stroke model.
MeSH term(s)	Mice ; Animals ; Neurons/metabolism ; Stroke/metabolism ; Axons/pathology ; Microscopy, Fluorescence, Multiphoton/methods ; Presynaptic Terminals ; Neuronal Plasticity/physiology ; Dendritic Spines/physiology
Language	English
Publishing date	2023-01-30
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2926-0_8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Conference proceedings: Novel biophotonic techniques and applications II

Pavone, Francesco

Novel biophotonic techniques and applications II, 12 - 14 May 2013, Munich, Germany ; [at the European Conference on Biomedical Optics (ECBO), part of World of Photonics Congress in Europe], Vitkin, Alex. - Bellingham, Wash. : SPIE

(Proceedings of OSA Biomedical Optics-SPIE ; 8804 ; Progess in biomedical optics and imaging ; Vol. 14, No. 54)

2013

Title variant	ECBO conference
Event/congress	European Conference on Biomedical Optics (ECBO) (2013.05.12-16, Munich) ; Neurophotonics (2013.05.12, Munich) ; Neurophotonics (2013.05.14, Munich) ; SPIE/OSA European Conferences on Biomedical Optics (ECBO) (2013.05.12-16, Munich)
Series title	Proceedings of OSA Biomedical Optics-SPIE ; 8804 Progess in biomedical optics and imaging ; Vol. 14, No. 54
Document type	Article ; Conference proceedings
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article ; Online: Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy.

Sorelli, Michele / Costantini, Irene / Bocchi, Leonardo / Axer, Markus / Pavone, Francesco Saverio / Mazzamuto, Giacomo

Scientific reports

2023 Volume 13, Issue 1, Page(s) 4160

Abstract: Fluorescence microscopy can be exploited for evaluating the brain's fiber architecture with unsurpassed spatial resolution in combination with different tissue preparation and staining protocols. Differently from state-of-the-art polarimetry-based ... ...

Abstract	Fluorescence microscopy can be exploited for evaluating the brain's fiber architecture with unsurpassed spatial resolution in combination with different tissue preparation and staining protocols. Differently from state-of-the-art polarimetry-based neuroimaging modalities, the quantification of fiber tract orientations from fluorescence microscopy volume images entails the application of specific image processing techniques, such as Fourier or structure tensor analysis. These, however, may lead to unreliable outcomes as they do not isolate myelinated fibers from the surrounding tissue. In this work, we describe a novel image processing pipeline that enables the computation of accurate 3D fiber orientation maps from both grey and white matter regions, exploiting the selective multiscale enhancement of tubular structures of varying diameters provided by a 3D implementation of the Frangi filter. The developed software tool can efficiently generate orientation distribution function maps at arbitrary spatial scales which may support the histological validation of modern diffusion-weighted magnetic resonance imaging tractography. Despite being tested here on two-photon scanning fluorescence microscopy images, acquired from tissue samples treated with a label-free technique enhancing the autofluorescence of myelinated fibers, the presented pipeline was developed to be employed on all types of 3D fluorescence images and fiber staining.
MeSH term(s)	Brain/diagnostic imaging ; Algorithms ; Image Processing, Computer-Assisted/methods ; Diffusion Magnetic Resonance Imaging/methods ; Microscopy, Fluorescence
Language	English
Publishing date	2023-03-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-30953-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Imaging Approaches to Investigate Pathophysiological Mechanisms of Brain Disease in Zebrafish.

Turrini, Lapo / Roschi, Lorenzo / de Vito, Giuseppe / Pavone, Francesco Saverio / Vanzi, Francesco

International journal of molecular sciences

2023 Volume 24, Issue 12

Abstract: Zebrafish has become an essential model organism in modern biomedical research. Owing to its distinctive features and high grade of genomic homology with humans, it is increasingly employed to model diverse neurological disorders, both through genetic ... ...

Abstract	Zebrafish has become an essential model organism in modern biomedical research. Owing to its distinctive features and high grade of genomic homology with humans, it is increasingly employed to model diverse neurological disorders, both through genetic and pharmacological intervention. The use of this vertebrate model has recently enhanced research efforts, both in the optical technology and in the bioengineering fields, aiming at developing novel tools for high spatiotemporal resolution imaging. Indeed, the ever-increasing use of imaging methods, often combined with fluorescent reporters or tags, enable a unique chance for translational neuroscience research at different levels, ranging from behavior (whole-organism) to functional aspects (whole-brain) and down to structural features (cellular and subcellular). In this work, we present a review of the imaging approaches employed to investigate pathophysiological mechanisms underlying functional, structural, and behavioral alterations of human neurological diseases modeled in zebrafish.
MeSH term(s)	Animals ; Humans ; Zebrafish/genetics ; Disease Models, Animal ; Brain Diseases/diagnostic imaging ; Brain/diagnostic imaging ; Brain/physiology ; Nervous System Diseases/diagnostic imaging
Language	English
Publishing date	2023-06-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24129833
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Article: Tracking the Effect of Therapy With Single-Trial Based Classification After Stroke.

Scaglione, Alessandro / Conti, Emilia / Allegra Mascaro, Anna Letizia / Pavone, Francesco Saverio

Frontiers in systems neuroscience

2022 Volume 16, Page(s) 840922

Abstract: Stroke is a debilitating disease that leads, in the 50% of cases, to permanent motor or cognitive impairments. The effectiveness of therapies that promote recovery after stroke depends on indicators of the disease state that can measure the degree of ... ...

Abstract	Stroke is a debilitating disease that leads, in the 50% of cases, to permanent motor or cognitive impairments. The effectiveness of therapies that promote recovery after stroke depends on indicators of the disease state that can measure the degree of recovery or predict treatment response or both. Here, we propose to use single-trial classification of task dependent neural activity to assess the disease state and track recovery after stroke. We tested this idea on calcium imaging data of the dorsal cortex of healthy, spontaneously recovered and rehabilitated mice while performing a forelimb retraction task. Results show that, at a single-trial level for the three experimental groups, neural activation during the reward pull can be detected with high accuracy with respect to the background activity in all cortical areas of the field of view and this activation is quite stable across trials and subjects of the same group. Moreover, single-trial responses during the reward pull can be used to discriminate between healthy and stroke subjects with areas closer to the injury site displaying higher discrimination capability than areas closer to this site. Finally, a classifier built to discriminate between controls and stroke at the single-trial level can be used to generate an index of the disease state, the therapeutic score, which is validated on the group of rehabilitated mice. In conclusion, task-related neural activity can be used as an indicator of disease state and track recovery without selecting a peculiar feature of the neural responses. This novel method can be used in both the development and assessment of different therapeutic strategies.
Language	English
Publishing date	2022-05-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2453005-0
ISSN	1662-5137
ISSN	1662-5137
DOI	10.3389/fnsys.2022.840922
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Article ; Online: Optimization of highly inclined illumination for diffraction-limited and super-resolution microscopy.

Gardini, Lucia / Vignolini, Tiziano / Curcio, Valentina / Pavone, Francesco Saverio / Capitanio, Marco

Optics express

2023 Volume 31, Issue 16, Page(s) 26208–26225

Abstract: In HILO microscopy, a highly inclined and laminated light sheet is used to illuminate the sample, thus drastically reducing background fluorescence in wide-field microscopy, but maintaining the simplicity of the use of a single objective for both ... ...

Abstract	In HILO microscopy, a highly inclined and laminated light sheet is used to illuminate the sample, thus drastically reducing background fluorescence in wide-field microscopy, but maintaining the simplicity of the use of a single objective for both illumination and detection. Although the technique has become widely popular, particularly in single molecule and super-resolution microscopy, a limited understanding of how to finely shape the illumination beam and of how this impacts on the image quality complicates the setting of HILO to fit the experimental needs. In this work, we build up a simple and comprehensive guide to optimize the beam shape and alignment in HILO and to predict its performance in conventional fluorescence and super-resolution microscopy. We model the beam propagation through Gaussian optics and validate the model through far- and near-field experiments, thus characterizing the main geometrical features of the beam. Further, we fully quantify the effects of a progressive reduction of the inclined beam thickness on the image quality of both diffraction-limited and super-resolution images and we show that the most relevant impact is obtained by reducing the beam thickness to sub-cellular dimensions (< 3 µm). Based on this, we present a simple optical solution that exploits a rectangular slit to reduce the inclined beam thickness down to 2.6 µm while keeping a field-of-view dimension suited for cell imaging and allowing an increase in the number of localizations in super-resolution imaging of up to 2.6 folds.
Language	English
Publishing date	2023-09-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	1491859-6
ISSN	1094-4087 ; 1094-4087
ISSN (online)	1094-4087
ISSN	1094-4087
DOI	10.1364/OE.492152
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Article ; Online: Photothrombotic Middle Cerebral Artery Occlusion in Mice: A Novel Model of Ischemic Stroke.

Conti, Emilia / Carlini, Noemi / Piccardi, Benedetta / Allegra Mascaro, Anna Letizia / Pavone, Francesco Saverio

eNeuro

2023 Volume 10, Issue 2

Abstract: Stroke is one of the main causes of death and disability worldwide. Over the past decades, several animal models of focal cerebral ischemia have been developed allowing to investigate pathophysiological mechanisms underlying stroke progression. Despite ... ...

Abstract	Stroke is one of the main causes of death and disability worldwide. Over the past decades, several animal models of focal cerebral ischemia have been developed allowing to investigate pathophysiological mechanisms underlying stroke progression. Despite intense preclinical research efforts, the need for noninvasive mouse models of vascular occlusion targeting the middle cerebral artery yet avoiding mechanical intervention is still pressing. Here, by applying the photothrombotic stroke model to the distal branch of the middle cerebral artery, we developed a novel strategy to induce a targeted occlusion of a large blood vessel in mice. This approach induces unilateral damage encompassing most of the dorsal cortex from the motor up to the visual regions 1 week after stroke. Pronounced limb dystonia one day after the damage is partially recovered after one week. Furthermore, we observe the insurgence of blood vessel leakage and edema formation in the peri-infarct area. Finally, this model elicits a notable inflammatory response revealed as a strong increase in astrocyte density and morphologic complexity in the perilesional region of the cortex compared with both other regions of the ipsilesional and contralesional hemispheres, and in sham-operated mice. To conclude, the stroke model we developed induces in mice the light-mediated occlusion of one of the main targets of human ischemic stroke, the middle cerebral artery, free from the limitations of commonly used preclinical models.
MeSH term(s)	Mice ; Humans ; Animals ; Infarction, Middle Cerebral Artery/complications ; Ischemic Stroke/complications ; Stroke/complications ; Brain Ischemia/complications ; Middle Cerebral Artery/surgery ; Disease Models, Animal
Language	English
Publishing date	2023-02-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2800598-3
ISSN	2373-2822 ; 2373-2822
ISSN (online)	2373-2822
ISSN	2373-2822
DOI	10.1523/ENEURO.0244-22.2022
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Microplastics reach the brain and interfere with honey bee cognition.

Pasquini, Elisa / Ferrante, Federico / Passaponti, Leonardo / Pavone, Francesco Saverio / Costantini, Irene / Baracchi, David

The Science of the total environment

2023 Volume 912, Page(s) 169362

Abstract: Scientific research on the impact of microplastics (MPs) in terrestrial systems is still emerging, but it has confirmed adverse health effects in organisms exposed to plastics. Although recent studies have shown the toxicological effects of individual ... ...

Abstract	Scientific research on the impact of microplastics (MPs) in terrestrial systems is still emerging, but it has confirmed adverse health effects in organisms exposed to plastics. Although recent studies have shown the toxicological effects of individual MPs polymers on honey bees, the effects of different polymer combinations on cognitive and behavioural performance remain unknown. To fill this knowledge gap, we investigated the effects of oral exposure to spherical MPs on cognitive performance and brain accumulation in the honey bee Apis mellifera. We evaluated the acute toxicity, after a two-day exposure, of polystyrene (PS - 4.8-5.8 μm) and plexiglass (Poly(methyl methacrylate), or PMMA - 1-40 μm) MPs, and a combination of the two (MIX), at two environmentally relevant and one higher concentration (0.5, 5 and 50 mg L
MeSH term(s)	Bees ; Animals ; Microplastics/toxicity ; Plastics/toxicity ; Polymethyl Methacrylate ; Polystyrenes ; Brain ; Cognition ; Sucrose
Chemical Substances	Microplastics ; Plastics ; Polymethyl Methacrylate (9011-14-7) ; Polystyrenes ; Sucrose (57-50-1)
Language	English
Publishing date	2023-12-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2023.169362
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Article ; Online: Mapping brain state-dependent sensory responses across the mouse cortex.

Montagni, Elena / Resta, Francesco / Tort-Colet, Núria / Scaglione, Alessandro / Mazzamuto, Giacomo / Destexhe, Alain / Pavone, Francesco Saverio / Allegra Mascaro, Anna Letizia

iScience

2024 Volume 27, Issue 5, Page(s) 109692

Abstract: Sensory information must be integrated across a distributed brain network for stimulus processing and perception. Recent studies have revealed specific spatiotemporal patterns of cortical activation for the early and late components of sensory-evoked ... ...

Abstract	Sensory information must be integrated across a distributed brain network for stimulus processing and perception. Recent studies have revealed specific spatiotemporal patterns of cortical activation for the early and late components of sensory-evoked responses, which are associated with stimulus features and perception, respectively. Here, we investigated how the brain state influences the sensory-evoked activation across the mouse cortex. We utilized isoflurane to modulate the brain state and conducted wide-field calcium imaging of Thy1-GCaMP6f mice to monitor distributed activation evoked by multi-whisker stimulation. Our findings reveal that the level of anesthesia strongly shapes the spatiotemporal features and the functional connectivity of the sensory-activated network. As anesthesia levels decrease, we observe increasingly complex responses, accompanied by the emergence of the late component within the sensory-evoked response. The persistence of the late component under anesthesia raises new questions regarding the potential existence of perception during unconscious states.
Language	English
Publishing date	2024-04-09
Publishing country	United States
Document type	Journal Article
ISSN	2589-0042
ISSN (online)	2589-0042
DOI	10.1016/j.isci.2024.109692
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