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Article ; Online: Antioxidant effects of silver nanoparticles obtained by green synthesis from the aqueous extract of Eryngium carlinae on the brain mitochondria of streptozotocin-induced diabetic rats.

Lemus-de la Cruz, Jenaro / Trejo-Hurtado, Mitchell / Landa-Moreno, Cinthia / Peña-Montes, Donovan / Landeros-Páramo, José Luis / Cortés-Rojo, Christian / Montoya-Pérez, Rocío / Rosas, Gerardo / Saavedra-Molina, Alfredo

Journal of bioenergetics and biomembranes

2023 Volume 55, Issue 2, Page(s) 123–135

Abstract: Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia that affects practically all tissues and organs, being the brain one of most susceptible, due to overproduction of reactive oxygen species induced by diabetes. Eryngium ... ...

Abstract	Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia that affects practically all tissues and organs, being the brain one of most susceptible, due to overproduction of reactive oxygen species induced by diabetes. Eryngium carlinae is a plant used in traditional Mexican medicine to treat diabetes, which has already been experimentally shown have hypoglycemic, antioxidant and hypolipidemic properties. The green synthesis of nanoparticles is a technique that combines plant extracts with metallic nanoparticles, so that the nanoparticles reduce the absorption and distribution time of drugs or compounds, increasing their effectiveness. In this work, the antioxidant effects and mitochondrial function in the brain were evaluated, as well as the hypoglycemic and hypolipidemic effect in serum of both the aqueous extract of the aerial part of E. carlinae, as well as its combination with silver nanoparticles of green synthesis. Administration with both, extract and the combination significantly decreased the production of reactive oxygen species, lipid peroxidation, and restored the activity of superoxide dismutase 2, glutathione peroxidase, and electron transport chain complexes in brain, while that the extract-nanoparticle combination decreased blood glucose and triglyceride levels. The results obtained suggest that both treatments have oxidative activity and restore mitochondrial function in the brain of diabetic rats.
MeSH term(s)	Rats ; Animals ; Antioxidants/pharmacology ; Antioxidants/metabolism ; Streptozocin/metabolism ; Streptozocin/pharmacology ; Streptozocin/therapeutic use ; Eryngium/metabolism ; Silver/pharmacology ; Silver/metabolism ; Silver/therapeutic use ; Reactive Oxygen Species/metabolism ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/metabolism ; Metal Nanoparticles ; Oxidative Stress ; Rats, Wistar ; Hypoglycemic Agents/pharmacology ; Hypoglycemic Agents/therapeutic use ; Hypoglycemic Agents/metabolism ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Lipid Peroxidation ; Brain/metabolism ; Mitochondria/metabolism
Chemical Substances	Antioxidants ; Streptozocin (5W494URQ81) ; Silver (3M4G523W1G) ; Reactive Oxygen Species ; Hypoglycemic Agents ; Plant Extracts
Language	English
Publishing date	2023-03-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	198499-8
ISSN	1573-6881 ; 0145-479X ; 0449-5705
ISSN (online)	1573-6881
ISSN	0145-479X ; 0449-5705
DOI	10.1007/s10863-023-09963-w
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Article ; Online: Diazoxide improves muscle function in association with improved dyslipidemia and decreased muscle oxidative stress in streptozotocin-induced diabetic rats.

Vargas-Vargas, Manuel Alejandro / Saavedra-Molina, Alfredo / Gómez-Barroso, Mariana / Peña-Montes, Donovan / Cortés-Rojo, Christian / Rodríguez-Orozco, Alain R / Rocío, Montoya-Pérez

Journal of bioenergetics and biomembranes

2023 Volume 55, Issue 1, Page(s) 71–78

Abstract: Aim/introduction: Diabetes Mellitus is a chronic degenerative disease, and its main biochemical characteristic is hyperglycemia due to impaired insulin secretion, resistance to peripheral actions of insulin, or both. Hyperglycemia causes dyslipidemia ... ...

Abstract	Aim/introduction: Diabetes Mellitus is a chronic degenerative disease, and its main biochemical characteristic is hyperglycemia due to impaired insulin secretion, resistance to peripheral actions of insulin, or both. Hyperglycemia causes dyslipidemia and stimulates oxidative damage, leading to the main symptoms, such as fatigue and culminates in diabetic complications. Previous studies have shown that ATP-sensitive potassium channels counteract muscle fatigue and metabolic stress in healthy mouse models. To determine the effect of diazoxide on muscle strength development during diabetes, we tested the effect of diazoxide in streptozotocin-diabetic rats in muscle function, lipid profile and oxidative stress biomarkers. Materials and methods: Wistar rats were divided into 4 groups of six animals each: (1) Control group, (2) diabetes group, (3) Control group + diazoxide, and (4) Diabetic + diazoxide (DB + DZX). 4 weeks after rats were sacrificed, soleus and extensor digitorum longus muscles (EDL) were extracted to prepare homogenates and serum was obtained for biochemical measurements. Oxidative damage was evaluated by the thiobarbituric acid method and the fluorescent for reactive oxygen species (ROS) probe 2,4-H Results: Diabetic rats with diazoxide administration showed an increase in the development of muscle strength in both muscles; in turn, the onset of fatigue was longer compared to the group of diabetic rats without treatment. Regarding the lipid profile, diazoxide decreased total cholesterol levels in the group of diabetic rats treated with diazoxide (x̅46.2 mg/dL) compared to the untreated diabetic group (x̅=104.4 mg/dL); secondly, diazoxide decreased triglyceride concentrations (x̅=105.3 mg/dL) compared to the untreated diabetic rats (x̅=412.2 mg/dL) as well as the levels of very low-density lipoproteins (x̅=20.4 mg/dL vs. x̅=82.44 mg/dL). Regarding the various markers of oxidative stress, the diabetic group treated with diazoxide was able to reduce the concentrations of TBARS and total reactive oxygen species as well as preserve the concentrations of reduced glutathione. Conclusion: Diazoxide administration in diabetic rats increases muscle strength development in EDL and soleus muscle, decreases fatigue, reduces cholesterol and triglyceride concentrations and improves oxidative stress parameters such as TBARS, ROS, and glutathione status.
MeSH term(s)	Mice ; Rats ; Animals ; Diazoxide/adverse effects ; Diazoxide/metabolism ; Streptozocin/adverse effects ; Streptozocin/metabolism ; Rats, Wistar ; Reactive Oxygen Species/metabolism ; Diabetes Mellitus, Experimental/complications ; Thiobarbituric Acid Reactive Substances/adverse effects ; Thiobarbituric Acid Reactive Substances/metabolism ; Oxidative Stress ; Hyperglycemia/complications ; Muscle, Skeletal/metabolism ; Lipids ; Triglycerides/adverse effects ; Triglycerides/metabolism ; Cholesterol/metabolism
Chemical Substances	Diazoxide (O5CB12L4FN) ; Streptozocin (5W494URQ81) ; Reactive Oxygen Species ; Thiobarbituric Acid Reactive Substances ; Lipids ; Triglycerides ; Cholesterol (97C5T2UQ7J)
Language	English
Publishing date	2023-02-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	198499-8
ISSN	1573-6881 ; 0145-479X ; 0449-5705
ISSN (online)	1573-6881
ISSN	0145-479X ; 0449-5705
DOI	10.1007/s10863-023-09958-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 1005: Show issues

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Jg. 1995 - 2021: Lesesall (1.OG)
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Article: Effects of Apocynin on Heart Muscle Oxidative Stress of Rats with Experimental Diabetes: Implications for Mitochondria.

Bravo-Sánchez, Estefanía / Peña-Montes, Donovan / Sánchez-Duarte, Sarai / Saavedra-Molina, Alfredo / Sánchez-Duarte, Elizabeth / Montoya-Pérez, Rocío

Antioxidants (Basel, Switzerland)

2021 Volume 10, Issue 3

Abstract: Diabetes mellitus (DM) constitutes one of the public health problems today. It is characterized by hyperglycemia through a defect in the β-cells function and/or decreased insulin sensitivity. Apocynin has been tasted acting directly as an NADPH oxidase ... ...

Abstract	Diabetes mellitus (DM) constitutes one of the public health problems today. It is characterized by hyperglycemia through a defect in the β-cells function and/or decreased insulin sensitivity. Apocynin has been tasted acting directly as an NADPH oxidase inhibitor and reactive oxygen species (ROS) scavenger, exhibiting beneficial effects against diabetic complications. Hence, the present study's goal was to dissect the possible mechanisms by which apocynin could mediate its cardioprotective effect against DM-induced oxidative stress. Male Wistar rats were assigned into 4 groups: Control (C), control + apocynin (C+A), diabetes (D), diabetes + apocynin (D+A). DM was induced with streptozotocin. Apocynin treatment (3 mg/kg/day) was applied for 5 weeks. Treatment significantly decreased blood glucose levels and insulin resistance in diabetic rats. In cardiac tissue, ROS levels were higher, and catalase enzyme activity was reduced in the D group compared to the C group; the apocynin treatment significantly attenuated these responses. In heart mitochondria, Complexes I and II of the electron transport chain (ETC) were significantly enhanced in the D+A group. Total glutathione, the level of reduced glutathione (GSH) and the GSH/ oxidized glutathione (GSSG) ratio were increased in the D+A group. Superoxide dismutase (SOD) and the glutathione peroxidase (GSH-Px) activities were without change. Apocynin enhances glucose uptake and insulin sensitivity, preserving the antioxidant defense and mitochondrial function.
Language	English
Publishing date	2021-02-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox10030335
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Article: Hypolipidemic and Antioxidant Effects of

Esquivel-Gutiérrez, Edgar R / Manzo-Avalos, Salvador / Peña-Montes, Donovan J / Saavedra-Molina, Alfredo / Morreeuw, Zoé P / Reyes, Ana G

Plants (Basel, Switzerland)

2021 Volume 10, Issue 11

Abstract: In the present study, we used a by-product ... ...

Abstract	In the present study, we used a by-product from
Language	English
Publishing date	2021-11-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants10112492
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Article: Hypolipidemic and Antioxidant Effects of Guishe Extract from Agave lechuguilla, a Mexican Plant with Biotechnological Potential, on Streptozotocin-Induced Diabetic Male Rats

Esquivel-Gutiérrez, Edgar R. / Manzo-Avalos, Salvador / Peña-Montes, Donovan J. / Saavedra-Molina, Alfredo / Morreeuw, Zoé P. / Reyes, Ana G.

Plants. 2021 Nov. 18, v. 10, no. 11

2021

Abstract: In the present study, we used a by-product from Agave lechuguilla (guishe) to test its antidiabetic effect, hypolipidemic activity, and capacity to mitigate the oxidative stress in kidney mitochondria from streptozotocin-induced diabetic rats. Orally, a ... ...

Abstract	In the present study, we used a by-product from Agave lechuguilla (guishe) to test its antidiabetic effect, hypolipidemic activity, and capacity to mitigate the oxidative stress in kidney mitochondria from streptozotocin-induced diabetic rats. Orally, a crude aqueous extract from lyophilized guishe was administered over 5 weeks at different doses. Blood glucose and body weight were monitored. Also, blood chemistry, bilirubin, and alanine aminotransferase were assayed. Furthermore, the activity of catalase, thiobarbituric acid reactive species, mitochondrial superoxide dismutase, glutathione and glutathione peroxidase were determined in isolated kidney mitochondria. Our results show that guishe extracts have no antidiabetic properties at any dose. Nevertheless, it was able to diminish serum triglyceride levels and regulate the oxidative stress observed in isolated kidney mitochondria. These observations indicate that the aqueous extract from guishe can be used to treat abnormalities in serum lipids, as a hypolipidemic, and mitigate the oxidative stress, as an antioxidant, occurring during diabetes.
Keywords	Agave lechuguilla ; alanine transaminase ; antioxidants ; bilirubin ; blood glucose ; blood serum ; body weight ; byproducts ; catalase ; diabetes ; freeze drying ; glutathione ; glutathione peroxidase ; glycemic effect ; kidneys ; males ; mitochondria ; oxidative stress ; superoxide dismutase ; thiobarbituric acid-reactive substances ; triacylglycerols
Language	English
Dates of publication	2021-1118
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants10112492
Database	NAL-Catalogue (AGRICOLA)

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Article: Effects of Apocynin on Heart Muscle Oxidative Stress of Rats with Experimental Diabetes: Implications for Mitochondria

Bravo-Sánchez, Estefanía / Peña-Montes, Donovan / Sánchez-Duarte, Sarai / Saavedra-Molina, Alfredo / Sánchez-Duarte, Elizabeth / Montoya-Pérez, Rocío

Antioxidants. 2021 Feb. 24, v. 10, no. 3

2021

Abstract	Diabetes mellitus (DM) constitutes one of the public health problems today. It is characterized by hyperglycemia through a defect in the β-cells function and/or decreased insulin sensitivity. Apocynin has been tasted acting directly as an NADPH oxidase inhibitor and reactive oxygen species (ROS) scavenger, exhibiting beneficial effects against diabetic complications. Hence, the present study’s goal was to dissect the possible mechanisms by which apocynin could mediate its cardioprotective effect against DM-induced oxidative stress. Male Wistar rats were assigned into 4 groups: Control (C), control + apocynin (C+A), diabetes (D), diabetes + apocynin (D+A). DM was induced with streptozotocin. Apocynin treatment (3 mg/kg/day) was applied for 5 weeks. Treatment significantly decreased blood glucose levels and insulin resistance in diabetic rats. In cardiac tissue, ROS levels were higher, and catalase enzyme activity was reduced in the D group compared to the C group; the apocynin treatment significantly attenuated these responses. In heart mitochondria, Complexes I and II of the electron transport chain (ETC) were significantly enhanced in the D+A group. Total glutathione, the level of reduced glutathione (GSH) and the GSH/ oxidized glutathione (GSSG) ratio were increased in the D+A group. Superoxide dismutase (SOD) and the glutathione peroxidase (GSH-Px) activities were without change. Apocynin enhances glucose uptake and insulin sensitivity, preserving the antioxidant defense and mitochondrial function.
Keywords	NAD(P)H oxidase (H2O2-forming) ; antioxidant activity ; blood glucose ; cardioprotective effect ; catalase ; electron transport chain ; enzyme activity ; glucose ; glutathione ; glutathione peroxidase ; hyperglycemia ; insulin resistance ; males ; mitochondria ; myocardium ; oxidative stress ; public health ; reactive oxygen species ; streptozotocin ; superoxide dismutase
Language	English
Dates of publication	2021-0224
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox10030335
Database	NAL-Catalogue (AGRICOLA)

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Article: An Ethyl Acetate Extract of

Trejo-Hurtado, Cristian M / Landa-Moreno, Cinthia I / la Cruz, Jenaro Lemus-de / Peña-Montes, Donovan J / Montoya-Pérez, Rocío / Salgado-Garciglia, Rafael / Manzo-Avalos, Salvador / Cortés-Rojo, Christian / Monribot-Villanueva, Juan Luis / Guerrero-Analco, José Antonio / Saavedra-Molina, Alfredo

Antioxidants (Basel, Switzerland)

2023 Volume 12, Issue 6

Abstract: Secondary metabolites such as flavonoids are promising in the treatment of non-alcoholic fatty liver disease (NAFLD), which is one of the complications of diabetes due to oxidative stress and inflammation. Some plants, such ... ...

Abstract	Secondary metabolites such as flavonoids are promising in the treatment of non-alcoholic fatty liver disease (NAFLD), which is one of the complications of diabetes due to oxidative stress and inflammation. Some plants, such as
Language	English
Publishing date	2023-06-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox12061235
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Article: Dietary Iron Restriction Improves Muscle Function, Dyslipidemia, and Decreased Muscle Oxidative Stress in Streptozotocin-Induced Diabetic Rats.

Vargas-Vargas, Manuel Alejandro / Saavedra-Molina, Alfredo / Gómez-Barroso, Mariana / Peña-Montes, Donovan / Cortés-Rojo, Christian / Miguel, Huerta / Trujillo, Xochitl / Montoya-Pérez, Rocío

Antioxidants (Basel, Switzerland)

2022 Volume 11, Issue 4

Abstract: Diabetes mellitus is a chronic degenerative disease characterized by hyperglycemia and oxidative stress. Iron catalyzes free radical overproduction. High iron concentrations have previously been reported to promote an increase in oxidative stress; ... ...

Abstract	Diabetes mellitus is a chronic degenerative disease characterized by hyperglycemia and oxidative stress. Iron catalyzes free radical overproduction. High iron concentrations have previously been reported to promote an increase in oxidative stress; however, the effect of iron restriction in diabetes has not yet been explored, so we tested to see if iron restriction in diabetic rats reduces oxidative damage and improved muscle function. Wistar rats were assigned to 4 groups: Control; Diabetic; Diabetic rats with a high iron diet, and Diabetic with dietary iron restriction. After 8 weeks the rats were sacrificed, the muscles were extracted to prepare homogenates, and serum was obtained for biochemical measurements. Low iron diabetic rats showed an increase in the development of muscle strength in both muscles. Dietary iron restriction decreased triglyceride concentrations compared to the untreated diabetic rats and the levels of extremely low-density lipoproteins. Aggravation of lipid peroxidation was observed in the diabetic group with a high iron diet, while these levels remained low with iron restriction. Iron restriction improved muscle strength development and reduced fatigue times; this was related to better lipid profile control and decreased oxidant stress markers.
Language	English
Publishing date	2022-04-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox11040731
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Article: Comparative Effect of Three Different Exercise Intensities in Combination with Diazoxide on Contraction Capacity and Oxidative Stress of Skeletal Muscle in Obese Rats.

Gómez-Barroso, Mariana / Vargas-Vargas, Manuel A / Peña-Montes, Donovan J / Cortés-Rojo, Christian / Saavedra-Molina, Alfredo / Sánchez-Duarte, Elizabeth / Rodríguez-Orozco, Alain R / Montoya-Pérez, Rocío

Biology

2022 Volume 11, Issue 9

Abstract: Obesity is a chronic disease that impairs skeletal muscle function, affects the ability to contract, and promotes the development of fatigue. For this reason, the study of treatments that seek to reduce the harmful effects of obesity on muscle tissue has ...

Abstract	Obesity is a chronic disease that impairs skeletal muscle function, affects the ability to contract, and promotes the development of fatigue. For this reason, the study of treatments that seek to reduce the harmful effects of obesity on muscle tissue has been deepened. Diazoxide treatment and various exercise protocols have been proposed to protect skeletal muscle against oxidative stress and its effects. However, the intensity and duration of exercise combined with diazoxide that would obtain the best results for improving skeletal muscle function in obese rats is unknown. To this end, this study evaluated the effects of three different exercise intensities combined with diazoxide on contraction capacity, resistance to fatigue, markers of oxidative stress, lipid peroxidation, ROS, and glutathione redox status of skeletal muscle. The results showed that treatments with diazoxide and exercise at different intensities improved muscle contraction capacity by reducing oxidative stress during obesity, with the best results being obtained with low-intensity exercise in combination with diazoxide. Therefore, these results suggest that diazoxide and low-intensity exercise improve muscle function during obesity by decreasing oxidative stress with the same efficiency as a moderate-intensity exercise protocol.
Language	English
Publishing date	2022-09-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology11091367
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Article ; Online: Antilipidemic and Hepatoprotective Effects of Ethanol Extract of

Murillo-Villicaña, Marina / Noriega-Cisneros, Ruth / Peña-Montes, Donovan J / Huerta-Cervantes, Maribel / Aguilera-Méndez, Asdrubal / Cortés-Rojo, Christian / Salgado-Garciglia, Rafael / Montoya-Pérez, Rocío / Riveros-Rosas, Héctor / Saavedra-Molina, Alfredo

Nutrients

2022 Volume 14, Issue 9

Abstract: Oxidative stress is a factor that contributes to the development of complications in diabetes; however, its effects can be counteracted using exogenous antioxidants that are found in some plants, which is why people turn to traditional medicines in the ... ...

Abstract	Oxidative stress is a factor that contributes to the development of complications in diabetes; however, its effects can be counteracted using exogenous antioxidants that are found in some plants, which is why people turn to traditional medicines in the search for therapeutic treatment.
MeSH term(s)	Animals ; Antioxidants/pharmacology ; Antioxidants/therapeutic use ; Blood Glucose ; Diabetes Mellitus, Experimental/drug therapy ; Ethanol/pharmacology ; Humans ; Hypoglycemic Agents/pharmacology ; Hypoglycemic Agents/therapeutic use ; Justicia ; Male ; Oxidative Stress ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Rats ; Rats, Wistar ; Streptozocin
Chemical Substances	Antioxidants ; Blood Glucose ; Hypoglycemic Agents ; Plant Extracts ; Ethanol (3K9958V90M) ; Streptozocin (5W494URQ81)
Language	English
Publishing date	2022-05-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu14091946
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