LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article ; Online: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues

    Lohana, Parkash / Suryaprawira, Albert / Woods, Emma L. / Dally, Jordanna / Gait-Carr, Edward / Alaidaroos, Nadia Y. A. / Heard, Charles M. / Lee, Kwok Y. / Ruge, Fiona / Farrier, Jeremy N. / Enoch, Stuart / Caley, Matthew P. / Peake, Matthew A. / Davies, Lindsay C. / Giles, Peter J. / Thomas, D. W. / Stephens, Phil / Moseley, Ryan

    Antioxidants. 2023 June 30, v. 12, no. 7

    2023  

    Abstract: Unlike skin, oral mucosal wounds are characterized by rapid healing and minimal scarring, attributable to the “enhanced” healing properties of oral mucosal fibroblasts (OMFs). As oxidative stress is increasingly implicated in regulating wound healing ... ...

    Abstract Unlike skin, oral mucosal wounds are characterized by rapid healing and minimal scarring, attributable to the “enhanced” healing properties of oral mucosal fibroblasts (OMFs). As oxidative stress is increasingly implicated in regulating wound healing outcomes, this study compared oxidative stress biomarker and enzymic antioxidant profiles between patient-matched oral mucosal/skin tissues and OMFs/skin fibroblasts (SFs) to determine whether superior oral mucosal antioxidant capabilities and reduced oxidative stress contributed to these preferential healing properties. Oral mucosa and skin exhibited similar patterns of oxidative protein damage and lipid peroxidation, localized within the lamina propria/dermis and oral/skin epithelia, respectively. SOD1, SOD2, SOD3 and catalase were primarily localized within epithelial tissues overall. However, SOD3 was also widespread within the lamina propria localized to OMFs, vasculature and the extracellular matrix. OMFs were further identified as being more resistant to reactive oxygen species (ROS) generation and oxidative DNA/protein damage than SFs. Despite histological evaluation suggesting that oral mucosa possessed higher SOD3 expression, this was not fully substantiated for all OMFs examined due to inter-patient donor variability. Such findings suggest that enzymic antioxidants have limited roles in mediating privileged wound healing responses in OMFs, implying that other non-enzymic antioxidants could be involved in protecting OMFs from oxidative stress overall.
    Keywords DNA ; biomarkers ; catalase ; dermis ; extracellular matrix ; fibroblasts ; histology ; lipid peroxidation ; mucosa ; oxidative stress ; reactive oxygen species
    Language English
    Dates of publication 2023-0630
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox12071374
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Role of Enzymic Antioxidants in Mediating Oxidative Stress and Contrasting Wound Healing Capabilities in Oral Mucosal/Skin Fibroblasts and Tissues.

    Lohana, Parkash / Suryaprawira, Albert / Woods, Emma L / Dally, Jordanna / Gait-Carr, Edward / Alaidaroos, Nadia Y A / Heard, Charles M / Lee, Kwok Y / Ruge, Fiona / Farrier, Jeremy N / Enoch, Stuart / Caley, Matthew P / Peake, Matthew A / Davies, Lindsay C / Giles, Peter J / Thomas, David W / Stephens, Phil / Moseley, Ryan

    Antioxidants (Basel, Switzerland)

    2023  Volume 12, Issue 7

    Abstract: Unlike skin, oral mucosal wounds are characterized by rapid healing and minimal scarring, attributable to the "enhanced" healing properties of oral mucosal fibroblasts (OMFs). As oxidative stress is increasingly implicated in regulating wound healing ... ...

    Abstract Unlike skin, oral mucosal wounds are characterized by rapid healing and minimal scarring, attributable to the "enhanced" healing properties of oral mucosal fibroblasts (OMFs). As oxidative stress is increasingly implicated in regulating wound healing outcomes, this study compared oxidative stress biomarker and enzymic antioxidant profiles between patient-matched oral mucosal/skin tissues and OMFs/skin fibroblasts (SFs) to determine whether superior oral mucosal antioxidant capabilities and reduced oxidative stress contributed to these preferential healing properties. Oral mucosa and skin exhibited similar patterns of oxidative protein damage and lipid peroxidation, localized within the lamina propria/dermis and oral/skin epithelia, respectively. SOD1, SOD2, SOD3 and catalase were primarily localized within epithelial tissues overall. However, SOD3 was also widespread within the lamina propria localized to OMFs, vasculature and the extracellular matrix. OMFs were further identified as being more resistant to reactive oxygen species (ROS) generation and oxidative DNA/protein damage than SFs. Despite histological evaluation suggesting that oral mucosa possessed higher SOD3 expression, this was not fully substantiated for all OMFs examined due to inter-patient donor variability. Such findings suggest that enzymic antioxidants have limited roles in mediating privileged wound healing responses in OMFs, implying that other non-enzymic antioxidants could be involved in protecting OMFs from oxidative stress overall.
    Language English
    Publishing date 2023-06-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704216-9
    ISSN 2076-3921
    ISSN 2076-3921
    DOI 10.3390/antiox12071374
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Identification of a transcriptional signature for the wound healing continuum.

    Peake, Matthew A / Caley, Mathew / Giles, Peter J / Wall, Ivan / Enoch, Stuart / Davies, Lindsay C / Kipling, David / Thomas, David W / Stephens, Phil

    Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

    2014  Volume 22, Issue 3, Page(s) 399–405

    Abstract: There is a spectrum/continuum of adult human wound healing outcomes ranging from the enhanced (nearly scarless) healing observed in oral mucosa to scarring within skin and the nonhealing of chronic skin wounds. Central to these outcomes is the role of ... ...

    Abstract There is a spectrum/continuum of adult human wound healing outcomes ranging from the enhanced (nearly scarless) healing observed in oral mucosa to scarring within skin and the nonhealing of chronic skin wounds. Central to these outcomes is the role of the fibroblast. Global gene expression profiling utilizing microarrays is starting to give insight into the role of such cells during the healing process, but no studies to date have produced a gene signature for this wound healing continuum. Microarray analysis of adult oral mucosal fibroblast (OMF), normal skin fibroblast (NF), and chronic wound fibroblast (CWF) at 0 and 6 hours post-serum stimulation was performed. Genes whose expression increases following serum exposure in the order OMF < NF < CWF are candidates for a negative/impaired healing phenotype (the dysfunctional healing group), whereas genes with the converse pattern are potentially associated with a positive/preferential healing phenotype (the enhanced healing group). Sixty-six genes in the enhanced healing group and 38 genes in the dysfunctional healing group were identified. Overrepresentation analysis revealed pathways directly and indirectly associated with wound healing and aging and additional categories associated with differentiation, development, and morphogenesis. Knowledge of this wound healing continuum gene signature may in turn assist in the therapeutic assessment/treatment of a patient's wounds.
    MeSH term(s) Adult ; Cell Proliferation ; Chronic Disease ; Cicatrix/genetics ; Cicatrix/pathology ; Female ; Fibroblasts/pathology ; Gene Expression ; Gene Expression Profiling ; Humans ; Leg Ulcer/pathology ; Male ; Microarray Analysis ; Mouth Mucosa/pathology ; Skin/pathology ; Wound Healing/genetics
    Language English
    Publishing date 2014-05-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1174873-4
    ISSN 1524-475X ; 1067-1927
    ISSN (online) 1524-475X
    ISSN 1067-1927
    DOI 10.1111/wrr.12170
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3890: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Preliminary characterisation of mechanoresponsive regions of the c-fos promoter in bone cells.

    Peake, Matthew A / El Haj, Alicia J

    FEBS letters

    2002  Volume 537, Issue 1-3, Page(s) 117–120

    Abstract: Preliminary analysis of the mechanisms involved in induction of stretch-mediated transcriptional activity in the c-fos promoter of bone has been undertaken using a series of c-fos promoter-reporter constructs. UMR-106 osteoblastic cells transfected with ... ...

    Abstract Preliminary analysis of the mechanisms involved in induction of stretch-mediated transcriptional activity in the c-fos promoter of bone has been undertaken using a series of c-fos promoter-reporter constructs. UMR-106 osteoblastic cells transfected with reporter constructs were subjected to cyclical physiological loading. The major determinants in the resulting transcriptional mechanoactivation are within the sequence between -356 and -151 which contains the serum response element and a consensus shear stress response element. Elements beyond this region also play a role as deletion of this region does not eliminate mechanoinduction. These results suggest that the mechanical induction of c-fos in osteoblastic bone cells is mediated by multiple response elements.
    MeSH term(s) Animals ; Binding Sites ; Bone Neoplasms ; Cell Line ; Chloramphenicol O-Acetyltransferase/genetics ; Genes, Reporter ; Genes, fos ; Osteoblasts/physiology ; Osteosarcoma ; Plasmids ; Promoter Regions, Genetic ; Proto-Oncogene Proteins c-fos/chemistry ; Proto-Oncogene Proteins c-fos/genetics ; Rats ; Recombinant Fusion Proteins/metabolism ; Stress, Mechanical ; Transcription, Genetic ; Transfection ; Tumor Cells, Cultured
    Chemical Substances Proto-Oncogene Proteins c-fos ; Recombinant Fusion Proteins ; Chloramphenicol O-Acetyltransferase (EC 2.3.1.28)
    Language English
    Publishing date 2002-07-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1016/s0014-5793(03)00108-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 282: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top