LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article: Assessing and benchmarking multiphoton microscopes for biologists.

    Corbin, Kaitlin / Pinkard, Henry / Peck, Sebastian / Beemiller, Peter / Krummel, Matthew F

    Methods in cell biology

    2014  Volume 123, Page(s) 135–151

    Abstract: Multiphoton microscopy has become staple tool for tracking cells within tissues and organs due to superior depth of penetration, low excitation volumes, and reduced phototoxicity. Many factors, ranging from laser pulse width to relay optics to detectors ... ...

    Abstract Multiphoton microscopy has become staple tool for tracking cells within tissues and organs due to superior depth of penetration, low excitation volumes, and reduced phototoxicity. Many factors, ranging from laser pulse width to relay optics to detectors and electronics, contribute to the overall ability of these microscopes to excite and detect fluorescence deep within tissues. However, we have found that there are few standard ways already described in the literature to distinguish between microscopes or to benchmark existing microscopes to measure the overall quality and efficiency of these instruments. Here, we discuss some simple parameters and methods that can either be used within a multiphoton facility or by a prospective purchaser to benchmark performance. This can both assist in identifying decay in microscope performance and in choosing features of a scope that are suited to experimental needs.
    MeSH term(s) Benchmarking ; Cells, Cultured ; Green Fluorescent Proteins/biosynthesis ; Green Fluorescent Proteins/chemistry ; Humans ; Microscopy, Fluorescence, Multiphoton/instrumentation ; Microscopy, Fluorescence, Multiphoton/standards ; Signal-To-Noise Ratio ; Single-Cell Analysis/instrumentation ; Single-Cell Analysis/standards
    Chemical Substances Green Fluorescent Proteins (147336-22-9)
    Language English
    Publishing date 2014-06-25
    Publishing country United States
    Document type Journal Article
    ISSN 0091-679X
    ISSN 0091-679X
    DOI 10.1016/B978-0-12-420138-5.00008-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: The septin cytoskeleton facilitates membrane retraction during motility and blebbing.

    Gilden, Julia K / Peck, Sebastian / Chen, Yi-Chun M / Krummel, Matthew F

    The Journal of cell biology

    2011  Volume 196, Issue 1, Page(s) 103–114

    Abstract: Increasing evidence supports a critical role for the septin cytoskeleton at the plasma membrane during physiological processes including motility, formation of dendritic spines or cilia, and phagocytosis. We sought to determine how septins regulate the ... ...

    Abstract Increasing evidence supports a critical role for the septin cytoskeleton at the plasma membrane during physiological processes including motility, formation of dendritic spines or cilia, and phagocytosis. We sought to determine how septins regulate the plasma membrane, focusing on this cytoskeletal element's role during effective amoeboid motility. Surprisingly, septins play a reactive rather than proactive role, as demonstrated during the response to increasing hydrostatic pressure and subsequent regulatory volume decrease. In these settings, septins were required for rapid cortical contraction, and SEPT6-GFP was recruited into filaments and circular patches during global cortical contraction and also specifically during actin filament depletion. Recruitment of septins was also evident during excessive blebbing initiated by blocking membrane trafficking with a dynamin inhibitor, providing further evidence that septins are recruited to facilitate retraction of membranes during dynamic shape change. This function of septins in assembling on an unstable cortex and retracting aberrantly protruding membranes explains the excessive blebbing and protrusion observed in septin-deficient T cells.
    MeSH term(s) Actomyosin/metabolism ; Actomyosin/physiology ; Animals ; Biological Transport ; Cell Line ; Cell Membrane/physiology ; Cell Membrane/ultrastructure ; Cell Movement ; Cytoskeleton/metabolism ; Cytoskeleton/physiology ; Cytoskeleton/ultrastructure ; Green Fluorescent Proteins/analysis ; Mice ; Recombinant Fusion Proteins/analysis ; Septins/metabolism ; Septins/physiology
    Chemical Substances Recombinant Fusion Proteins ; Green Fluorescent Proteins (147336-22-9) ; Actomyosin (9013-26-7) ; Sept6 protein, mouse (EC 3.6.1.-) ; Septins (EC 3.6.1.-)
    Language English
    Publishing date 2011-12-15
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 218154-x
    ISSN 1540-8140 ; 0021-9525
    ISSN (online) 1540-8140
    ISSN 0021-9525
    DOI 10.1083/jcb.201105127
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ub I Zs.190: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Lack of the GTPase RHO-4 in Neurospora crassa causes a reduction in numbers and aberrant stabilization of microtubules at hyphal tips.

    Rasmussen, Carolyn G / Morgenstein, Randy M / Peck, Sebastian / Glass, N Louise

    Fungal genetics and biology : FG & B

    2008  Volume 45, Issue 6, Page(s) 1027–1039

    Abstract: The multinucleate hyphae of the filamentous ascomycete fungus Neurospora crassa grow by polarized hyphal tip extension. Both the actin and microtubule cytoskeleton are required for maximum hyphal extension, in addition to other vital processes. ... ...

    Abstract The multinucleate hyphae of the filamentous ascomycete fungus Neurospora crassa grow by polarized hyphal tip extension. Both the actin and microtubule cytoskeleton are required for maximum hyphal extension, in addition to other vital processes. Previously, we have shown that the monomeric GTPase encoded by the N. crassa rho-4 locus is required for actin ring formation during the process of septation; rho-4 mutants lack septa. However, other phenotypic aspects of the rho-4 mutant, such as slow growth and cytoplasmic bleeding, led us to examine the hypothesis that the microtubule (MT) cytoskeleton of the rho-4 mutant was affected in morphology and dynamics. Unlike a wild-type strain, the rho-4 mutant had few MTs and these few MTs originated from nuclear spindle pole bodies. rho-4 mutants and rho-4 strains containing a GTP-locked (activated) rho-4 allele showed a reduction in numbers of cytoplasmic MTs and microtubule stabilization at hyphal tips. Strains containing a GDP-biased (negative) allele of rho-4 showed normal numbers of MTs and minor effects on microtubule stabilization. An examination of nuclear dynamics revealed that rho-4 mutants have large, and often, stretched or broken nuclei. These observations indicate that RHO-4 plays important roles in regulating both the actin and MT cytoskeleton, which are essential for optimal hyphal tip growth and in nuclear distribution and morphology.
    MeSH term(s) Cell Nucleus/chemistry ; Cell Nucleus/metabolism ; Cytoplasm/chemistry ; Cytoplasm/metabolism ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; GTP Phosphohydrolases/genetics ; GTP Phosphohydrolases/metabolism ; Hyphae/chemistry ; Hyphae/cytology ; Hyphae/growth & development ; Hyphae/metabolism ; Microtubules/chemistry ; Microtubules/metabolism ; Neurospora crassa/chemistry ; Neurospora crassa/cytology ; Neurospora crassa/enzymology ; Neurospora crassa/genetics ; Ploidies
    Chemical Substances Fungal Proteins ; GTP Phosphohydrolases (EC 3.6.1.-)
    Language English
    Publishing date 2008-06
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1319820-8
    ISSN 1096-0937 ; 1087-1845 ; 0147-5975
    ISSN (online) 1096-0937
    ISSN 1087-1845 ; 0147-5975
    DOI 10.1016/j.fgb.2008.02.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2431: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Lack of the GTPase RHO-4 in Neurospora crassa causes a reduction in numbers and aberrant stabilization of microtubules at hyphal tips

    Rasmussen, Carolyn G / Morgenstein, Randy M / Peck, Sebastian / Glass, N. Louise

    Fungal genetics and biology : FG & B. 2008 June, v. 45, issue 6

    2008  

    Keywords Neurospora crassa ; guanosinetriphosphatase ; mutants ; phenotype ; microtubules ; cell biology ; hyphae ; cell nucleus
    Language English
    Dates of publication 2008-06
    Size p. 1027-1039.
    Document type Article
    ZDB-ID 1319820-8
    ISSN 1096-0937 ; 1087-1845 ; 0147-5975
    ISSN (online) 1096-0937
    ISSN 1087-1845 ; 0147-5975
    DOI 10.1016/j.fgb.2008.02.006
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 79/730: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top