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  1. Book ; Thesis: Identification and mapping of molecular markers associated with QTLs for kernel traits in pearl millet

    Pedraza-Garcia, Fabio

    2005  

    Author's details by Fabio Pedraza-Garcia
    Language English
    Size vi, 67 leaves :, ill. ;, 28 cm.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Thesis (M.S.)--University of Nebraska--Lincoln, 2005
    Database NAL-Catalogue (AGRICOLA)

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  2. Conference proceedings ; Online: Molecular marker for the apomixis gene in Brachiaria

    Miles, John W. / Pedraza García, Fabio / Palacios, N / Tohme, Joseph M.

    2015  

    Keywords brachiaria ; genetic markers ; apomixis ; marcadores genéticos
    Language English
    Publishing date 2015-05-04T09:17:21Z
    Publisher Town and Country Convention Center
    Publishing country fr
    Document type Conference proceedings ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Conference proceedings ; Online: Marcadores SCAR y RAPD para la resistencia a la bacteriosis común (CBB) del frijol

    Pedraza García, Fabio / Gallego Sánchez, Gerardo J. / Beebe, Stephen E. / Tohme, Joseph M.

    2017  

    Abstract: Para marcar los loci que determinan la resistencia al ataque de la bacteriosis común en frijol, se emplearon los polimorfismos en el ADN aleatoriamente amplificado (RAPD), junto con tres diferente metodológicas de análisis génico: análisis de los ... ...

    Abstract Para marcar los loci que determinan la resistencia al ataque de la bacteriosis común en frijol, se emplearon los polimorfismos en el ADN aleatoriamente amplificado (RAPD), junto con tres diferente metodológicas de análisis génico: análisis de los segregantes diferencialmente agrupados (BSA), selección marcadores mediante genotipos contrastantes y selección con base en los genotipos donadores de la característica génica de interés. Mediante estas t6cnicas y usando una población de cien líneas F8, recombinantes homocigotas originadas de plantas individuales F, provenientes del cruzamiento de DOR 476 x SEL 1309, se identificaron 33 decanucleótidos cebadores de la ADN-polimerasa (RAPD primers), que en conjunto amplificaron 84 fragmentos de ADN polimórficos para los parentales. Estos marcadores RAPD, fueron mapeados sobre 17 grupos de ligamiento (512,3 cM), uno de ellos (83,2 cM) conformado por seis marcadores RAPD, presentó el mayor efecto sobre las diferencias fenotípicas observadas en la reacción foliar al ataque de CBB. Del anterior conjunto se seleccionó el marcador con una fuerte influencia sobre la resistencia a bacteriosis, para diseñar un par de oligonucleótidos cebadores de 20 mer, que permitiesen la amplificación específica de dicho locus, bajo un condiciones de astringencia mayores a las usadas con el cebador RAPD, determinando de esta forma un marcador SCAR para resistencia a esta enfermedad. Se observó que este par de cebadores amplifican sólo un locus en el parental resistente (SEL 1309) y ninguno en el susceptible (DOR 476), este locus es también inequívocamente amplificado a partir de los genomas de XAN 159 y de la accesión G 40020 de P. acutifolius usada como fuente primaria de resistencia, confirmando los resultados obtenidos mediante la técnica RAPD. (RA)
    Keywords phaseolus vulgaris ; breeding ; genetic markers ; fitomejoramiento ; marcadores genéticos
    Language Spanish
    Publishing date 2017-03-14T08:46:02Z
    Publisher International Center for Tropical Agriculture
    Publishing country fr
    Document type Conference proceedings ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Comparison of genetic diversity between random samples from core and reserve collections of Phaseolus vulgaris L

    Nienhuis, James / Skroch, Paul W. / Beebe, Stephen E. / Tohme, Joseph M. / Pedraza García, Fabio

    2017  

    Keywords phaseolus vulgaris ; genetic variation ; germplasm ; variación genética ; germoplasma
    Language English
    Publishing date 2017-10-12T08:04:14Z
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: A genetic map of common bean combining RFLP, RAPD, SCAR and AFLP markers

    Beebe, Stephen E. / Pedraza García, Fabio / Rojas, Marcela / Gutiérrez A., Janeth Patricia / Tohme, Joseph M.

    2017  

    Keywords phaseolus vulgaris ; genetic maps ; genetic markers ; mapas genéticos ; marcadores genéticos
    Language English
    Publishing date 2017-10-12T08:04:24Z
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Legume microsatellites tested in common bean

    Blair, Matthew W. / Pantoja Delgado, W / Pedraza García, Fabio / Cregan, P.B. / Fatokun, C.A.

    2015  

    Keywords phaseolus vulgaris ; glycine max ; soybeans ; cajanus cajan ; genomes ; microsatellites ; leguminosae ; genetic distance ; soya ; genomas ; microsatélites ; distancia genética
    Language English
    Publishing date 2015-05-08T07:55:23Z
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Development of SCAR markers linked to common bacterial blight resistance genes (QTL) in common bean

    Jung, Geunhwua / Beebe, Stephen E. / Nienhuis, James / Park, Sung-Jo / Coyne, Dermot P. / Marita, J. / Pedraza García, Fabio / Tohme, Joseph M.

    2017  

    Keywords phaseolus vulgaris ; xanthomonas campestris ; genetic markers ; genetic resistance ; inbred lines ; marcadores genéticos ; resistencia genética ; líneas consanguíneas
    Language English
    Publishing date 2017-10-12T08:04:25Z
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Genetic mapping of the bean golden yellow mosaic geminivirus resistance gene bgm-1 and linkage with potyvirus resistance in common bean (Phaseolus vulgaris L.)

    Blair, Matthew W. / Rodríguez, L.M. / Pedraza García, Fabio / Morales, Francisco José / Beebe, Stephen E.

    Theoretical and Applied Genetics

    2014  

    Abstract: Bean golden yellow mosaic virus (BGYMV) is a whitefly-transmitted geminivirus of the Begomovirus family that causes important yield losses to common beans grown in tropical and sub-tropical countries of Latin America and the Caribbean. A major resistance ...

    Abstract Bean golden yellow mosaic virus (BGYMV) is a whitefly-transmitted geminivirus of the Begomovirus family that causes important yield losses to common beans grown in tropical and sub-tropical countries of Latin America and the Caribbean. A major resistance gene that has been widely deployed in this region is the recessive locus bgm-1 that prevents the development of severe yellowing typical of the disease. In this study, we developed a co-dominant sequence-characterized amplified region (SCAR) marker, SR2, based on a previously identified random amplified polymorphic DNA (RAPD) marker that is tightly linked to the bgm-1 resistance gene and identified the position of the locus in the common bean genome through comparative mapping using two genetic maps for the species. The SR2 marker was mapped relative to bgm-1 in a segregating population of recombinant inbred lines developed from the resistant × susceptible cross of DOR476 × SEL1309. Polymorphism was shown to be based on a 37 bp insertion event in the SR2 allele associated with susceptibility compared to the allele associated with resistance and the marker mapped at a distance of 7.8 cM from the resistance gene. The SR2 marker was significantly associated with overall disease symptoms and with three of the four symptoms associated with the disease (yellowing or chlorosis, flower abortion, foliar deformation) in a greenhouse trial in Colombia with the mechanically transmissible BGYMV–Guatemala strain. In both the DOR364 × G19833 and BAT93 × Jalo EEP558 mapping populations, SR2 was located near the end of linkage group b03 (chromosome 5) suggesting a sub-telomeric position. The position of the bgm-1 resistance gene was estimated to be close to that of bc-1, a strain-specific resistance gene for Bean common mosaic virus (BCMV), based on linkage of SR2 with the SCAR marker SBD5 in the DOR364 × G19833 mapping population. The implications of linkage between these two recessive resistance genes are discussed, as this is the first association between resistance genes ...
    Keywords phaseolus vulgaris ; geminiviruses ; potyviruses ; genetic maps ; genetic resistance ; genetic markers ; geminivirus ; potyvirus ; mapas genéticos ; resistencia genética ; marcadores genéticos ; genetics ; biotechnology
    Language English
    Publishing date 2014-09-24T07:58:44Z
    Publisher Springer
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Development and genetic mapping of a scar marker for the bean golden yellow mosaic geminivirus resistant gene BGM-1

    Blair, Matthew W. / Rodríguez, L.M. / Pedraza García, Fabio / Morales, Francisco José / Beebe, Stephen E. / Miklas, PN / Beaver, James S.

    2015  

    Keywords phaseolus vulgaris ; genetic maps ; genetic markers ; viruses ; genetic resistance ; mapas genéticos ; marcadores genéticos ; virus ; resistencia genética
    Language English
    Publishing date 2015-05-08T07:55:41Z
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Studies in Phaseolus germplasm diversity

    Beebe, Stephen E. / Tohme, Joseph M. / Nienhuis, James / Pedraza García, Fabio / Rengifo, J / Tovar Luque, E. / Islam, A.

    a review of work at CIAT

    2015  

    Keywords phaseolus vulgaris ; wild plants ; germplasm ; genetic markers ; rapd ; aflp ; gene pools ; genetic variation ; plantas silvestres ; germoplasma ; marcadores genéticos ; reservas genéticas ; variación genética
    Language English
    Publishing date 2015-05-08T07:55:28Z
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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